• Journal of Korean Public Health Nursing
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• v.25 no.2
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• pp.266-275
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• 2011

Purpose: The study investigated the relationship of health behavior with bone mineral density (BMD) for college students. Methods: A descriptive study was done using a convenient sampling of college students (n=116) enrolled in a health-related department. Height, weight, body mass index (BMI), health behaviors recognition of subjective health, smoking, drinking, exercise, food habits, intake of calcium, and family history of fracture were measured. BMD was measured at the right forearm using Hologic lunar duel-energy X-ray absorptiometry. Data were analyzed using descriptive analysis, t-test, ANOVA and multiple linear regression. Results: The prevalence of osteopenia and osteoporosis was 41.4% and 22.4%, respectively. Significant relationships were observed between BMD of right forearm and gender, BMI, subjective health recognition, drinking, and exercise (p<.05). Results of linear regression after adjusted BMD were increased concerning subjective health recognition and regular exercise (p<.05). Conclusions: Subjective health recognition and exercise carries positive effects on BMD. We recommend for college students that healthy behaviors like proper weight, smoking cessation, regular exercise, regular food habits, and health awareness are helpful to BMD.

• Biomolecules & Therapeutics
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• v.7 no.3
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• pp.216-220
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• 1999

A chemosensitivity assay with small replicate Mm5mt/cl C3H mammary tumor cell cultures was developed to determine whether changes in viral antigen expression and release into culture fluids could be utilized as an in vitro measure of modulating drug effect. The 52,000 MW viral envelope glycoprotein (gp52) of the mouse mammary tumor virus (MMTV) was measured in culture fluids of control and drug-treated cultures while cell density was simultaneously determined by cell staining and OD 664 nm determination. While extra-cellular gp52 levels and cell density progressively increased over 72 hours for control cultures, declines in both parameters provided dual measures of effect for combination [N(phophonacetyl-L-aspartic acid)+5-fluorouracil], combination 〔N(phophonacetyl-L-aspartic acid )+5-fluoro-5'-deoxyuridine〕and single component treatment of this combination. At each treated time point, thesecombinations begin to produce a greater decline in both cell density and gp52 levels as compared to single drug treatments. These results indicate that N(phopho-nacetyl-L-aspartic acid) in combination can enhance the effectiveness of single drug.

• Journal of Forest and Environmental Science
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• v.35 no.1
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• pp.54-60
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• 2019

Plant tissue culture techniques offer quick methods of regeneration of plants of medicinal importance but the survival chances of such plants are always questionable when shifted to the in vivo conditions. The present study enumerates the micromorphological developments in the leaves of in vitro regenerated and field transferred plantlets of Oldenlandia umbellata. The leaves developed in vitro after $4^{th}$ subcultures of multiplication phase and after 6 weeks of field transferred plants were used. Statistically significant differences in the number of stomata, veins, raphides, crystals and trichome density per square mm were observed. The improvements in stomatal apparatus and density (decreased from 41.85 to 32.20), developments in leaf architectural parameters and emergence of defense mechanism through increased numbers of raphides (8 to 15), crystals and trichomes (13.5 to 18.2) proved acclimation of tissue culture raised plantlets from in vitro to the in vivo environments lead to 100 % success in field establishment of the plantlets. The in vitro induced foliar abnormalities (changes in stomata, venation pattern, vein density, trichomes, crystals etc.) were repaired while hardening of plantlets in the greenhouse and finally in the field. The observed micromorphological response of leaves under altered environmental conditions could help in determination of proper stage of field transfer and prediction of survival percentage of in vitro regenerated O. umbellata plantlets.

• Journal of the Korean Society of Food Culture
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• v.22 no.6
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• pp.841-847
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• 2007

This study was conducted to investigate on bone density and nutrient intake of university students in Seoul area. Nutrient intake data were obtained by using the 24-hour recall method to evaluate the usual diet of the subjects. BQI(bone quality index) of the subjects was measured by an Quantitative Ultrasound (QUS). The results are summarized as follows: The average height, weight, BMI of the male and female student were 173.3 cm, 68.5 kg, 22.7; 161.4 cm, 54.2 kg, 20.8, respectively. The BQI and Z-score of the subjects were 99.50, -0.69 in male student group, and 82.6, -1.15 in female student group, respectively. Normal, osteopenia and osteoporosis percentage by bone status were 73.8%, 24.9%, 1.3% in male student group, and 39.8%, 57.6%, 2.6% in female student group, respectively. Energy intake of male and female group were 71.7%, 79.1% of EER(estimated energy requirement) respectively. Fiber, Ca, Vit $B_2$, niacin, folic acid, Vit C intake were less than RI(recommended intake) and protein, phosphorus intake were higher than RI in subjects. Nutrient intake were not significantly related with BQI in male and female groups generally.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.379-385
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• 2006

This experiment was carried out to investigate the optimum planting density in low fertilizing cultivation of machine transplanting in rice field of Honam Agricultural Research Institute, NICS for $2004{\sim}2005$. Sobibyeo which belongs to medium maturing variety and Nampyeongbyeo which belongs to medium-late maturing variety were transplanted on May 30. In this experiment, there was no significant difference in heading date between planting density and nitrogen fertilization rate, and heading dates were August 8 in Sobibyeo, and August 14 in Nampyeongbyeo respectively. In relation to lodging character, lodging Index was high where the nitrogen fertilization rate and planting density were high. As planting density increases, panicle number per $m^{2}$ increased irrespective of nitrogen fertilization rate. When nitrogen was 6 kg/10a, rice yield of Sobibyeo was more where planting density was 90 hill per $3.3m^{2}$, and that of Nampyeongbyeo was more where planting density was 80 hill per $3.3m^{2}$. When nitrogen was 9 kg/10a, rice yield of Sobibyeo was more where planting density was 100 hill per $3.3m^{2}$, and that of Nampyeongbyeo was more where planting density was 110 hill per $3.3m^{2}$. Head rice rate of brown rice was higher when planting density increased, and was higher at 6 kg/10a nitrogen rate than 9 kg/10a nitrogen rate in all varieties.

• The Korean Journal of Physiology
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• v.2 no.1
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• pp.31-38
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• 1968

Total body fat measurement by means of skinfold thickness was performed in 94 secondary school boys. Hydrostatic weighing was made on the same subject and corrected for residual volume in lung. Skinfold thicknesses at four sites, namely, back, waist, arm and abdomen, were compared with total body fat calculated from the equation given by Keys and Brozek and regression equation were computated. In 48 middle school boys aged between 13 and 16 years and 46 high school boys aged between 16 and 19 years, skinfold thicknesses increased at all 4 sites as age increased. The body density, however, showed different pattern. In middle school ages, body density showed little variation. Density in 13-14 years was 1.0608, in 14-15 years 1.0578 and in 15-16 years 1.0546. In high school ages density in-creased abruptly to 1.0703 (16 yr.), 1.0730 (17 yr.) and 1.0740 (18 yr.). Subsequently 15 years was a boundary for density change and total body fat change. In middle school boys as a group of 48 the observed values were: density: 1.0562, total body fat: 15.9%, mean skinfold thickness at four sites: 6.02 mm. The coefficient of correlation between mean skinfold thickness and density were r=-0.759 and r=0.781 with %fat. The regression equations were : Body $density=-0.00527{\times}Mean$ skinfold thickness (mm)+1.0879, %Fat=$1.933{\times}Mean$ skinfold thickness (mm)+4.26 In high school boys the values were: density: 1.0723, body fat: 10.4%, and mean skinfold thickness: 7.89 mm. Coefficients of correlation between mean skinfold thickness and density were r=-0.868 and r=0.855 with % fat. Thus, Body density=$-0.00365{\times}Mean$ skinfold thickness (mm)+1.1008, and %Fat=$1.326{\times}Mean$ skinfold thickness (mm)-0.064, were obtained. Although skinfold thicknesses at 4 sites showed a continuous increase in absolute value as age increased, relative growth of skinfold at specific site differed. On arm the growth of skinfold showed a decrease and on waist it showed an increase as compared to the mean thickness.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.215-219
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• 2003

This study was conducted to find optimum bacterial density for improving the efficiency of transformation mediated by Agrobacterium tumefaciens in apples. Regeneration(15％) and transformation frequency(10％) were increased in resuspension-culture density $A_{600}$ 1.3 from preculture density $A_{600}$ 0.7 of Agrobacterium tumefaciens in ′Fuji′. In ′Gala′, 20% regeneration and 16% transformation frequency were observed at optimum bacterial density $A_{600}$ 0.7 form preculture density $A_{600}$ 1.3. ′Mclntosh as well as "Gala" were 25％regeneration and 10％ transformation frequency. Hence a frequency optimum condition of bacterial density for the efficient transformation of apple could be depend on apple genotypes.

• Journal of Microbiology and Biotechnology
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• v.10 no.6
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• pp.784-788
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• 2000

Hypolipidemic effect of exo-polymers(EPs) form submerged mycelial culture of Cordyceps militaris was investigated in male Sprague-Dawley rats. For a dose-dependent study, EPs were administered at the level of 50-100 mg/kg body weight (BW) and the data was compared with the saline administered control group. A significant reduction of both the plasma total cholesterol (TC) and triglyceride (TG) was registered under the influence of EPs. It reduced the lowe density lipoprotein (LDL) cholesterol level as much as 40.5%. Levels of high density lipoprotein (HDL) choloesterol did not vary significantly within the different experimental groups, but the HDL: TC ratio showed consistently a high value with the increasing dose. The effects of cultural conditions (pH and temperature) in mycelial growth and EPs production were studied. Both the biomass and EPs were produced in a wide range of pH and temperature.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.972-977
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• 2004

The hypoglycemic effect of an exo-biopolymer (EXO) and endo-biopolymer (ENDO) produced from submerged mycelial culture of Ganoderma lucidum was investigated in streptozotocin (STZ)-induced diabetic rats. Both the EXO and ENDO showed hypoglycemic potential, however, the former proved to be more potent than the latter. The administration of the EXO at the dose of 100 mg/kg body weight (BW) significantly reduced the plasma glucose level (23.5%) and increased the plasma insulin level (2.2 fold) in the diabetic animals. The EXO also lowered the plasma total cholesterol, triglyceride, low-density lipoprotein (LDL) cholesterol, and athrogenic index by 14.7, 31.4, 24.1, and 45.4%, respectively, and reduced the liver total cholesterol and triglyceride levels by 6.7 and 25.8%, respectively. It increased the plasma high-density lipoprotein (HDL) cholesterol (37.7%), compared to the control group. Furthermore, the alanine transaminase (ALT) and aspartate transaminase (AST) showed lower activities in the EXO administered groups than the other experimental groups. Taken together, these results suggest that the exo-biopolymer may alleviate the blood glucose level by increased insulin secretion.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.406-410
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• 2002

A new spin filter consisting of $50{\mu}m$ (nominal pore size) depth fitters rolled on a stainless steel grid was developed, using Saccharomyces cerevisiae as a model suspension cell to evaluate the spin filter performance. In a 1.8-1 fermentor with a rotation speed of 300 rpm and perfusion rate of 4 ml/min, a cell concentration of 49 g/l and ethanol concentration of 45 g/l from 100 g/l glucose could be obtained in a perfusion culture. The major mechanisms for cell separation used by the large-pore spin filter appeared to be centrifugal force and pivotal movement of the cells in the spin filter.