• Title/Summary/Keyword: Culture Complex

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Enhancement of Cultivation Efficiency of Bifidobacterium longum Using Calcium Carbonate Buffer System (Calcium Carbonate Buffer System을 이용한 Bifidobacterium longum의 배양 효율 증대에 관한 연구)

  • Lee, Ki-Yong;Hwang, In-Bum;Heo, Tae-Ryeon
    • Korean Journal of Food Science and Technology
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    • v.29 no.1
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    • pp.126-132
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    • 1997
  • Calcium carbonate ($CaCO_3$) immobilized with alginate was studied as buffer system to enhance the cultivation efficiency of Bifidobacterium longum (ATCC 15707) which is inhibited at low pH. To test the bufferring effect of the immobilized $CaCO_3$ beads, pH value in each modified trypticase-proteose peptone-yeast (TPY) broth which is adjusted to pH 4.0 with acetic acid, lactic acid and complex solution of acetic and lactic acid, 3:2 (M:M) was tested by concentration of $CaCO_3$ bead and reaction time. The bufferring effect of $CaCO_3$ bead became higher with increasing the amount of $CaCO_3$ bead in the acidic solution. The growth rate of bifidobacteria and bufferring effect were examined in relation to the amount of $CaCO_3$ bead and concentration of glucose in the modified TPY media. The growth rate of bifidobacteria and bufferring effect were increased with increasing the amount of $CaCO_3$ bead and concentration of glucose. Also, the exponential time of bifidobacteria became longer with increasing the amount of $CaCO_3$ bead and concentration of glucose in the modified TPY media. When we observed the growth rate of bifidobacteria by the method of pH-controlled culture and $CaCO_3$ buffer system, the $CaCO_3$ buffer system was more effective than that of pH-controlled culture. Therefore, this $CaCO_3$ buffer system may be useful as a method to enhance of the cultivation efficiency of bifidobacteria.

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A Study on food Ecology According to Obesity Index of Elementary School Children in a High Socioeconomic Apartment Complex in Seoul (서울시내 고소득층 아파트단지 국민학교 어린이의 체격과 식생태에 관한 조사연구)

  • Kim, Ju-Hye;Kim, Bok-Hee;Kim, Hee-Kyung;Son, Sook-Mee;Mo, Su-Mi;Choi, Hay-Mie
    • Journal of the Korean Society of Food Culture
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    • v.8 no.3
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    • pp.275-287
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    • 1993
  • An ecological survey of food and nutrition was conducted in April and May, among 278 children, 6 to 12 years old, of the Youido elementary school, which offers no school lunch program, and is situated in Youido delta of Han River side of Seoul, known as the location of one of the socioeconomically high groups; Eighteen percent and 18.3% of subjects were proven to be overweight and obese, respectively. With regard to frequency of skipping breakfast, 28.4% of the subjects skipped breakfast often and 2.5% of subjects skipped breakfast every morning. Obese children showed good appetite and the time consumed for eating was shorter than those of normal or overweight children. Most serious anxiety among the mothers for those children was food habit of `eating rapidly' and `overeating'. Another striking observation was that 14.4% of the subjects ate only one side-dish with boiled rice, and such a pattern was more often found in the normal or slim group. Most of the subjects had their snacks after being back in home from school. Thirty-one percent of the children had snacks when they felt hungry, 69% had snacks for various reasons such as `habitually', `for unwinding', and `mother gives me snacks'. Thirty-eight percent of the boys and 19% of the girls had physical exercise everyday and overweight and obese children got exercise more frequently. But the time consumed for exercise was much more shorter than that of normal subjects. Most of the children liked fruits, meats, and biscuit, in contrast, lowest preferency was for vegetables, fermented fish products, and boiled rice mixed with other grains. Obese children experienced more kinds of food than normal or slim group. The study found that habits of overeating due to good appetite and eating rapidly were recognized as one of dominant reasons causing obesity among children. On the other hand, serious dietary problem of children of normal or slim group was eating less variety of food. Active nutrition education for both children and mothers will be recommended with joint participation of teachers.

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Natural herbal extract complex induces the degradation of alcohol and acetaldehyde and reduces the breath alcohol concentration (천연소재복합물의 알코올 및 아세트알데히드 분해활성 및 호기중 알코올 농도 감소)

  • Hwang, Ji Hong;Kim, Mi-Yeon
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.3
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    • pp.381-392
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    • 2020
  • This study is purposed to check up the natural 12 kinds of herbal extracts suitable for hangover and based on the results of contents of phenolic compounds, ABTS radical scavenging activity, alcohol dehydrogenase (ADH), and acetaldehyde dehydrogenase (ALDH). Selected 8 kinds of herbal extracts are blended according to the efficacy and the pearson's correlation between each content and activity. C. sinensis var. sinensis, P. densiflora Gnarl and P. lobata Ohwi showed excellent ADH activity, P. lobata Ohwi had a strong correlation between the content and efficacy, and C. sinensis var. sinensis, P. densiflora Gnarl had a negative correlation. Through the ADH and ALDH activity test of F.1 to F.7, the F.7 showed the highest synergic effect and selected as an optimal formulation. F.7 intake-group, the breath alcohol concentration was significantly reduced to 58% after 30 minutes and 27% after 120 minutes, compared to right after alcohol consumption. After alcohol consumption, there was a significant improvement effect (p<0.05) in tired and thirst in the intake group compared to the non-intake group.

Establishment and Characterization of Canine Mammary Gland Cancer Cell Lines (개 자연발생 유선종양 2종의 세포주 확립 및 특성분석)

  • Lee, Sun-Tae;Kweon, Oh-Kyeong;Kim, Wan-Hee
    • Journal of Veterinary Clinics
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    • v.27 no.3
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    • pp.232-239
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    • 2010
  • Two cell lines derived from spontaneous canine mammary gland tumors were established and characterized. Mammary gland tumors from 9 years old pug and 9 years old toy-poodle dogs were collected by aseptic surgical resection and primary culture was performed. The histopathologic examination of tumors revealed adenocarcinoma and complex carcinoma and two dogs died from metastasis of the tumors. The tumor cells were subcultured over 60 times for more than 1 year and morphological consistency maintained. Light microscopic examination, growth curve, doubling time calculation, xenotransplantation to female nude mice, immunohistochemistry for wide spectrum keratin, vimentin, $\alpha$-smooth muscle actin and cytokeratin 8 was performed for characterization. The cell lines exhibited polygonal, elongated cell shape and cytoplasmic bridge and doubling time of 47.1 hrs and 18.6 hrs, respectively. Subcutaneous xenotransplantation to nude mice of the cells produced localized palpable mass within 4 weeks in 4 of 5 and 5 of 5 nude mice, respectively. In immunohistochemical examination one cell line showed strong positive against wide spectrum keratin and cytokeratin 8 and the other cell line showed strong positive against smooth muscle actin and cytokeratin 8. Additional characterization would be possible by investigator's needs and the cell lines may be useful for in vivo and in vitro studies of canine mammary tumor and adjuvant therapies.

Survey on the Patterns of Fortified Food Consumption and Intake of Vitamins and Minerals in Fortified Foods by Elementary School and Middle-School Students in Korea (우리나라 일부 초등학생과 중학생의 영양강화식품 섭취 실태 및 영양강화식품을 통한 비타민과 무기질 섭취량 조사)

  • Kim, Sun-Hyo
    • Journal of the Korean Society of Food Culture
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    • v.26 no.3
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    • pp.295-306
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    • 2011
  • This study was performed to investigate patterns of fortified food (FF) consumption and intake of vitamins and minerals from FFs among 577 Korean children (12.4 years of age) who attended elementary or middle school. FFs eaten by children as a snack were surveyed using the food record method during 3 days, including 2 week days and one weekend. As a result, 114 FF items were eaten by the children, and several kinds of nutrients such as vitamin A, D, E, B complex, C, calcium (Ca), iron (Fe), and zinc (Zn) were fortified in these foods. Ca-FFs (65.8%) were most frequently consumed, followed by vitamin C-FFs (33.4%) and vitamin D-FFs (33.3%). The number of FF items in each food group was the most in the milk group (n=24, 21.0%), followed by the beverage group (n=19, 16.7%), and the cookie/bread/cake group (n=17, 14.9%). Fortified nutrients in FFs were in various combinations, but the major combination patterns were Ca, Ca plus vitamins, Ca plus vitamins plus other minerals, and Ca plus other minerals. Daily mean intakes of vitamins and minerals from the FFs were 66-300% more than those of the recommended nutrient intake (RNI ) or adequate intake (AI) for most vitamins and minerals. Daily maximum intakes (95th percentile) of vitamins and minerals from FFs were 1-15 times the RNI or AI for most vitamins and minerals. Vitamin and mineral consumption ratios from each FF group were different according to the kind of fortified nutrient. For example, vitamin C was mostly eaten in fortified beverages (46-54%), and Fe was mostly eaten in fortified cookie/breads/cakes (87%). The above results show that FF consumption varied widely among the children, and that most of the children's foods were fortified with several vitamins and minerals without a common rule; thus, subjects risked over consuming vitamins and minerals by eating FFs. Therefore, practical guideline on FF use for children's optimal nutrition and health should be provided through nutrition education.

Interface of Interactive Contents using Vision-based Body Gesture Recognition (비전 기반 신체 제스처 인식을 이용한 상호작용 콘텐츠 인터페이스)

  • Park, Jae Wan;Song, Dae Hyun;Lee, Chil Woo
    • Smart Media Journal
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    • v.1 no.2
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    • pp.40-46
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    • 2012
  • In this paper, we describe interactive contents which is used the result of the inputted interface recognizing vision-based body gesture. Because the content uses the imp which is the common culture as the subject in Asia, we can enjoy it with culture familiarity. And also since the player can use their own gesture to fight with the imp in the game, they are naturally absorbed in the game. And the users can choose the multiple endings of the contents in the end of the scenario. In the part of the gesture recognition, KINECT is used to obtain the three-dimensional coordinates of each joint of the limb to capture the static pose of the actions. The vision-based 3D human pose recognition technology is used to method for convey human gesture in HCI(Human-Computer Interaction). 2D pose model based recognition method recognizes simple 2D human pose in particular environment On the other hand, 3D pose model which describes 3D human body skeletal structure can recognize more complex 3D pose than 2D pose model in because it can use joint angle and shape information of body part Because gestures can be presented through sequential static poses, we recognize the gestures which are configured poses by using HMM In this paper, we describe the interactive content which is used as input interface by using gesture recognition result. So, we can control the contents using only user's gestures naturally. And we intended to improve the immersion and the interest by using the imp who is used real-time interaction with user.

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Perchlorate Removal by River Microorganisms in Industrial Complexes (산업단지지역 하천 미생물에 의한 퍼클로레이트 제거)

  • Jo, Kang-Ick;Ahn, Yeonghee
    • Korean Chemical Engineering Research
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    • v.52 no.1
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    • pp.92-97
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    • 2014
  • Perchlorate ($ClO_4^-$) is an emerging contaminant of soil/groundwater and surface water. $ClO_4^-$ has been shown to inhibit iodide uptake into the thyroid gland and cause a reduction in thyroid hormone production. $ClO_4^-$ is highly soluble and very stable in water. Biodegradation by $ClO_4^-$-reducing bacteria (PRB) is considered the most important factor in natural attenuation of $ClO_4^-$. Rivers in an industrial complex have potential to be contaminated with $ClO_4^-$ discharged from point or non-point sources. In this study, water samples were taken from the rivers running through the Gumi industrial complexes and used for batch test to analyze $ClO_4^-$-degradation potential of river microorganisms. The results of 83-h batch culture showed that $ClO_4^-$-removal efficiency of all samples was 0.77% or less without addition of an external electron ($e^-$) donor. However $ClO_4^-$-removal efficiency was higher when an $e^-$ donor (acetate, thiosulfate, $S^0$, or $F^0$) was added into the batch culture, showing up to 100% removal efficiency. The removal efficiency was various depending on type of $e^-$ donor and site of sampling. When acetate was used as an $e^-$ donor, the highest $ClO_4^-$-removal efficiency was observed among the $e^-$ donors used in this study, suggesting that activity of heterotrophic PRB was dominant. The results of this study provide basic information on natural attenuation of $ClO_4^-$ by river microorganisms. The information can be useful to prepare a strategy to enhance efficiency of $ClO_4^-$ biodegradation for in situ bioremediation.

The Emerging security initiatives and forecasting future social and natural environment changes (신흥안보 창발과 미래 사회 및 자연환경 변화예측)

  • Jung, Min-Sub;NamKung, Seung-Pil;Park, Sang-Hyuk
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.2
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    • pp.327-331
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    • 2020
  • In that this study is a subject and character of risk, emerging security covers non-military areas in addition to traditional military security: environmental security, human security, resource security, and cyber security. The rise of these risks is not only changing the phenomenon of the new expansion of security areas, but also the expansion of the number and scope of security entities and the aspect of security world politics. These risks are transnational security issues at the global level in terms of their nature and extent of the damage, as well as multi-layered ones that affect local and personal security issues at the regional and national levels. In addition to national actors, non-state actors such as international organizations, multinational corporations, and global civil society, and furthermore, technology and social systems themselves are causing risks. Therefore, to solve the new security problem, it is necessary to establish a middle-level and complex governance mechanism that is sought at the regional and global levels beyond the fragmented dimension of the occurrence of new security issues that have been overlooked in the existing frame of perception, and to predict and find ways to respond to new security paradigms that have been identified in a broader sense.

A Study on the Androgynous Phenomena in Contemporary Fashion from the Feminist's Viewpoint (페미니즘적 시각에서 본 현대복식의 앤드로지너스 현상에 관한 연구)

  • 김혜연
    • Journal of the Korean Society of Costume
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    • v.43
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    • pp.203-224
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    • 1999
  • As androgny presented by feminism still appear in contemporary fashion since mid 1980's feminism as a social phenomenon can be considered to habe influenced fashion history. This paper surveys the sex images of androgynous look that appeared in contemporary fashion into bisexual image and neutral images based on the feminist aspects of changes in contemporary fashion. As stated above bisexual images and neutral images of androgynous look shown in clothing are an attempt to reach one single integrated complete being by overacoming 'femininity' or 'masculinity' and combining the element of the two sexes. Consequently androgynous look creates new attraction by harmoniously coordinating characteristics of the two sexes instead of totally differentiating between masculinity and femininity that are represented in the form of clothing that has conceptual visual characteristics. Following are the conclusions of the reseach of this paper. First 'androgynous' as a feminist sex concept reflects the spirit of the times. Since the sex roles are divided and diversified and the concept of femininity is changing the concept of androgyny requiring both masculine and feminine characteristics in an essential sex concept for sucessful conduct of roles in a complex modern society. Second the integrated human image of androgynous look has led to a new culture with fashion trends that has been ahead of times by curing the functions of fashion to differentiate between men and women. Third androgynous look at the turn-of-the-centuy will not cease to exist but be a lasting fashion phenomenon while the sex concept presented by feminism has become the core to destrov the dichotomous fashion style of the 20th century. Fourth androgynous look is expressed in various manners in contemporary fashion since it shown how human beings accipt the adaptation method desperately required by the contemporary times. Androgynous look can be viewd as the desire for ultimate freedom that can be acquired by destroying the dichotomous sex concept and as the will to become a subject of the world as integrated human being. As we look into fashion culture creation of fashion is not the result of an accidental cause or an individual's work but the reflection of the spirit of the times. In this sense androgynous look introduced by a new sex concept to contemporary fashion has reflected the spirit of the times and led the cultural atmosphere and moreover it is an important fashion style that can characterize the contemporary times while lasting in the future.

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The enjoyment of way on Si-jo and Ga-sa in Joseon Dynasty (시조와 가사의 향유방식과 그 관련양상)

  • Ryoo, Hae-Choon
    • Sijohaknonchong
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    • v.44
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    • pp.165-195
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    • 2016
  • This paper is compare to the enjoyment of way on Si-jo and Ga-sa in Lyric poetry. Si-jo and Ga-sa were created in Joseon Dynasty. Si-jo and Ga-sa is the Lyric poetry in traditional genre, representing Korea. These forms include the lyric genre but have a difference between the fixed verse and the long-poem in Joseon Dynasty. However, the two genres are the two genres had opened the possibility of communication from the very beginning in terms of gentry layer is the main writers. Joseon Dynasty those who enjoy the fixed verse and the long-poem, the literature of singing songs and reciting literature, in terms of the principle of metaphor and metonymy rhetoric will reflect the era of awareness and enjoyment of contemporary perception of reality characters. It can be said that performing Si-jo and Ga-sa of enjoyment fashion and work to compare with realistic about the versification principles but check ahead a lot of data, and add to the task of finding a logic that can explain clearly remain. This paper will have to complement the many points made in an attempt to unravel the complex issues in an integrated and comprehensive on-pronged approach and enjoyment of the fixed verse and long-poem Si-jo and Ga-sa and similar expressions are writing and testing the various parties of the enjoyment of culture in today's era of change in the 21st century can be the compass to navigate a new world culture.

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