• BMB Reports
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• v.32 no.4
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• pp.385-392
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• 1999

The $NAD^+$-specific activity of a dual coenzyme-specific isocitrate dehydrogenase (IDH; EC 1.1.1.41) from the primitive fungus Pythium ultimum was investigated to elucidate the regulatory factors that may influence the intracellular distribution of carbon and the availability of intermediates, e.g. citrate, for fatty acid synthesis. Inhibition of $NAD^+$-IDH activity by diphospho- and triphosphonucleotides (ATP, ADP, and GTP) reflected the sensitivity of this enzyme to cellular energy charge even though monophosphonucleotides (AMP and GMP) had little effect on activity. NADPH, but not NADH, substantially inhibited $NAD^+$-IDH activity, showing noncompetitive inhibition with isocitrate. Oxalacetate and ${\alpha}$-ketoglutarate showed competitive inhibition with isocitrate, while citrate and cis-aconitate showed mixed-noncompetitive inhibition with isocitrate. Inhibition by these substances ranged from 29 to 46% at 10 mM. The inhibitory effect of oxalacetate was increased synergistically by glyoxylate, which alone caused 31% uncompetitive inhibition at 10 mM, and a mixture of the two substances at 1 mM each showed 98% inhibition of $NAD^+$-IDH activity. The regulation of $NAD^+$-IDH in Pythium ultimum seems to be a complex process involving mitochondrial metabolites. The addition of glyoxylate (3 mM) and oxalacetate (3 mM) to the culture medium resulted in the production of 49% more lipid by P. ultimum.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.11a
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• pp.216-220
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• 2007

By virtue of recent economic development and cultural progress, the desires willing to enjoy a plentiful leisure life among people are on enlarging, then according to this, the demand for a grand performance hall where various cultural events are achievable is also on increasing. However, since the grand performance hall has its characteristics that must accommodate various forms of performances likely from lecture, meeting activity to recital, and because the clearness of sound is on emphasizing, and also it requires the repletion or abundance of sound simultaneously, it could be said that from the designing stage, a sufficient examination and plan with regard to the architectural acoustics design is indispensable. On such view point, for the object of the grand performance hall under actually planning to build, this Study is attempting to compare analyze on Room Acoustics Characteristics using computer simulation from the stage of design. It is considered that the material analyzed in such way could be reflected in maximum basis to the design of the performance hall that minimizes the acoustic defect at the designing step, and also it could be utilized as the useful material at the time of design for a similar form of performance hall in the future.

• Journal of Soil and Groundwater Environment
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• v.15 no.4
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• pp.13-20
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• 2010

The purpose of this study was to develope the remediation method of contaminated soils with metals and petroleum. The diesel degrading strain was isolated and identified from the soil contaminated by petroleum at industrial sites. Diesel biodegradation experiment was performed by diesel degrading bacteria in both solution and soil slurry. Contaminated soils by Zn or As and diesel were treated consecutively by steam-vapor extraction, biodegradation, and acid washing. The strain was identified as Pseudomonas aeruginosa, and named as Pseudomonas aeruginosa TPH1. The optimal culture conditions of TPH1 were $20^{\circ}C$ and pH 7.0, 3% of diesel concentration. Biodegradation of diesel was performed using the separated strain in liquid medium, and 63% of diesel was degraded in 72 hours. And 52% of diesel was removed in the tested soils. In the treatment of contaminated soils with diesel and Zn or As, 29% ~ 44% of diesel was reduced by steamvapor extraction, 60% ~ 71% of diesel was removed after biodegradation. 47% of Zn and 96% of As were removed after acid(mixture of sulfuric and oxalic acids) washing. It is recommended that consecutive treatment method of steam-vapor extraction, biodegradation and acid washing is effective for remediation of complex contaminated soils with metals and petroleum.

• Journal of the Korean Society of Costume
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• v.26
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• pp.123-143
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• 1995

Clothing as one of elements of culture has been interwoven with cultural diffusion, and accompanied the most visible change. In this paper, it was focused that the process and the characteristics of western clothing adop-tion of Korea from 1876 to 1945 corelating with cultural diffusion theory. They were analyzed through the change of clothing reformation system by government, school uniform, and social phenomenon. The finding of this paper were as followings; 1. The process of western clothing adoption was forcibly demanded by Japan, therefore influenced by Japan. 2. The clothing reformation which was forced to accept western style was confronted by complex of cultural, psychological and economical resistance. 3. The fashion leaders of this period were Korean students studying abroad, diplomatic officials, members of the armed forces, government officials, students of western educational systemed school, and lady of evangelist. 4. Man adopted western clothing earlier than woman. 5. Western clothing adoption was took precedence in case of formal wear, diplomatic official's attire, military uniform, and school uniform. 6. In this process, we can find 'transculturation' by Malinowski and 'reinter-pretation' by Herskovits. 7. This process was a kind of 'reorientaion'. 8. The change of clothing which was affected by the tradition, for example, robe for the ancestral rites was evolutionary than others. 9. Clothing elements based on mental or internal characteristics like which clothing was hardly changed by compulsion or extortion. 10. The external trends of clothing change during this period were simplicity, utility, and decrease of status symbols.

• Korean Institute of Interior Design Journal
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• v.24 no.6
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• pp.137-144
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• 2015

Light was the symbol of the city's prosperity and culture. People can walk around the city at the night time with safe. Now light is used with beauty and function at the same time. This study is the example how to draw the lighting design concept in specific city and district based on the citizen-minded survey. The citizen-minded survey makes a new chance taking the goodness and characteristics from local city specially and select the site for applying the specific opinion and requirement in the city. The expression of design concept on night landscape design gives the fresh image to the city during the night time. Because people feel the free and dynamic mood in the night landscape lighting, it is very important to set and express the design concept on night landscape design. The result of this study was applied in the Daegu city. In particular, the distribution complex's night landscape lighting in the Daegu was designed with specific concept expression from the citizen-minded survey. Making and expressing the design concept on night landscape design is basic for unity and diversity in the city.

• Microbiology and Biotechnology Letters
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• v.36 no.4
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• pp.353-359
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• 2008

Hepatitis C virus (HCV) is known as the causative agent of blood transmitted hepatitis. Two viral proteins, E2 and NS5A, are known to exert interferon resistance of HCV via PKR pathway. Here, we report a third protein, the RNA-dependent RNA polymerase (NS5B) of HCV, induced interferon resistance inhibiting p56 pathway. p56 was shown to interact with p48 subunit of eukaryotic initiation factor 3 (eIF3). This interaction inhibited formation of ternary complex in translation initiation. Using dual reporter assay system, we observed that the translation decreased when interferon alpha was added to the culture. But, in the presence of HCV NS5B, the translation partly recovered. NS5B and p48 subunit of eIF3 were shown to interact. This interaction seems to inhibit the interaction between p48 and p56. This is the first report that a virus exerts interferon resistance via p56 pathway.

• Microbiology and Biotechnology Letters
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• v.46 no.1
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• pp.45-50
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• 2018

The chitosanase gene (btbchito) of Bacillus thuringiensis BMB171 was cloned and heterologously expressed in the yeast Pichia pastoris. After purification, about 300 mg of recombinant chitosanase was obtained from the 1-1 culture medium with a specific activity of 240 units/mg. Results determined by the combined use of thin layer chromatography (TLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) showed that the chitooligosaccharides (COSs) obtained by chitosan (N-deacetylated by 70%, 80%, and 90%) hydrolysis by rBTBCHITO were comprised of oligomers, with degrees of polymerization (DP) mainly ranging from trimers to heptamers; high molecular weight chitopentaose, chitohexaose, and chitoheptaose were also produced. Hydrolysis products was also deduced using MS since the COSs (n) are complex oligosaccharides with various acetyl groups from one to two, so the non-acetyl COSs (GlcN)n and COSs with more acetyls (> 2) were not detected. The employment of this method in the production of high molecular weight COSs may be useful for various industrial and biological applications, and the activity of chitosanase has great significance in research and other applications.

• Journal of Biomedical Engineering Research
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• v.28 no.3
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• pp.377-386
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• 2007

Mechanical loading to bone cells using simple sine wave or constant wave fluid flow has been widely used for in vitro experiments. Human gait is characterized by a complex loading to bones of lower extremities which results from a series of events consisting of heel strike, foot flat and push-off during the stance phase of the gait cycle. Telemetric force analyses have shown that human femora are subject to multiphasic loading. Therefore, it would be ideal if the physiologic loading conditions during human walking can be used for in vitro mechanotransduction studies. Here, for a mechanotransduction study, we develop it fluid flow system (FFS) in order to simulate human physiologic mechanicalloading on bone cells. The development methods of the FFS including the COR (Center for Orthopedic Research), monitor program are presented. The FFS could generate various multiphasic loading conditions of human gaits with output flow. Wall shear distribution was very uniform, with 81 % of the effective loading area of the culture on a glass slide. Our results demonstrated that the FFS, provide a new translational approach for unveiling molecular mechanotransduction pathways in bone cells.

• Korean Institute of Interior Design Journal
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• v.17 no.6
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• pp.92-101
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• 2008

In the current age, as the importance of image is increased, brand images are strengthened, and various ways of expressing space are suggested considering the life styles of consumers. Recently, flagship stores are attempting various ways of expressing interior deigns and the flow of life culture, which can maximize the characteristics of image in a large, complex space. Centering on this trend, this study is conducted to explore an objective standard for the stylistic image characteristics reflecting the formative expression trend; to investigate various expression methods of images that are suggested by flagship stores; and to suggest the indicators of design. Based on this background, the formative trend is classified into the expression trends of minimalism and deconstructionism. An image evaluation test was conducted on the selected brand cases by applying the classification, and the following results are obtained from a comparative analysis. In this study, a stylistic analysis of expression methods is suggested from the stylistic image characteristics of each brand as well as from the reliability and validity of the expression trend. It can be concluded that such stylistic image characteristics are important factors in relation with fashion and architecture, and they can be used for differentiating design expression methods.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.442-449
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• 2004

Improving the light utilization efficiency of photosynthetic cells in photobioreactors (PBRs) is a major topic in algal biotechnology. Accordingly, in the current study we investigated the effect and suitability of photosynthetic pigment reduction for improving light utilization efficiency. The light-harvesting complex II (LH-II) genes of Rhodobacter sphaeroides were removed to construct a mutant strain with less pigment content. The mutant strain exhibited a slower growth rate than the wild-type under a low light intensity, while the mutant grew faster under a high light intensity. In addition, the specific absorption coefficient was lower in the mutant due to its reduced pigment content, thus it seemed that light penetrated deeper into its culture broth. However, the distance (light penetration depth) from the surface of the PBR to the compensation point did not increase, due to an increase in the compensation irradiance of the mutant strain. Experimental data showed that a reduced photosynthetic pigment content, which lessened the photoinhibition under high-intensity light, helped the volumetric productivity of photosynthetic microorganisms.