• Title/Summary/Keyword: Culture Collection

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Isolation of Lactobacillus plantarum from Kimchi and Its Inhibitory Activity on the Adherence and Growth of Helicobacter pylori

  • Lee, Hak-Mee;Lee, Yeon-Hee
    • Journal of Microbiology and Biotechnology
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    • v.16 no.10
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    • pp.1513-1517
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    • 2006
  • One single lactic acid producing bacterium, isolated from kimchi, inhibited the growth and adherence of Helicobacter pylori to the human gastric epithelial cell line MKN-45. This isolate was identified as Lactobacillus plantarum and termed L. plantarum strain PL9011. The adherence of H pylori, in the presence of live or nonviable L. plantarum strain PL9011 (10-fold CFU), decreased to 14-20%. The spent culture supernatant of L. plantarum strain PL9011 resulted in the eradication of H pylori. This activity remained stable following neutralization and heat treatment, but not following pepsin treatment, thereby suggesting small peptides as the inhibitory factor. L. plantarum strain PL9011 did not produce any harmful metabolites or enzymes. The results obtained in this study suggest that the L. plantarum strain PL9011 may be a potential novel probiotic for the stomach.

Enhanced ${\varepsilon}$-Poly-$_L$-lysine Production from Streptomyces ahygroscopicus by a Combination of Cell Immobilization and In Situ Adsorption

  • Liu, Shengrong;Wu, Qingping;Zhang, Jumei;Mo, Shuping;Yang, Xiaojuan;Xiao, Chun
    • Journal of Microbiology and Biotechnology
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    • v.22 no.9
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    • pp.1218-1223
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    • 2012
  • ${\varepsilon}$-Poly-$_L$-lysine (${\varepsilon}$-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of $_L$-lysine, which is used as a safe food preservative. The present study investigates the combined use of cell immobilization and in situ adsorption (ISA) to produce ${\varepsilon}$-PL in shaken flasks. Loofah sponge-immobilized Streptomyces ahygroscopicus GIM8 produced slightly more ${\varepsilon}$-PL than those immobilized on synthetic sponge, and sugarcane bagasse. Moreover, loofah sponge supported the maximum biomass. Hence, loofah sponge was chosen for cell immobilization. Meanwhile, the ion-exchange resin D152 was employed for ISA. The loofah sponge-immobilized cells produced $0.54{\pm}0.1g/l$ ${\varepsilon}$-PL, which significantly increased to $3.64{\pm}0.32g/l$ after combining with ISA through the addition of resin bags. The free cells with ISA using the dispersed resin yielded $2.73{\pm}0.26g/l$ of ${\varepsilon}$-PL, an increase from $0.82{\pm}0.08g/l$. These data illustrate that the proposed combination method improved production most significantly compared with either immobilization or ISA only. Moreover, the immobilized cells could be repeatedly used and an ${\varepsilon}$-PL total amount of $8.05{\pm}0.84g/l$ was obtained. The proposed combination method offers promising perspectives for ${\varepsilon}$-PL production.

The Types and Patterns of Nursing Organizational Culture (간호조직문화 유형 및 패턴에 관한 연구)

  • Kim, Min-Jung
    • Journal of Korean Academy of Nursing Administration
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    • v.12 no.2
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    • pp.265-275
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    • 2006
  • Purpose: The purpose of this study is to identify and to investigate the nursing organizational culture. Method: A descriptive correlational study was conducted with convenience sample of 448 staff nurses who were working for six general hospitals located in Seoul, Province of Gyeonggi and Province of Chung-cheong. The period of data collection took over 5 months between August and December. Organization Culture Questionnaire developed by Lee (2001) were used to measure nursing organizational culture. Collected data were analysed on SPSS Win 12.0. Result: The meta culture of the nursing organizations was the conservative culture. The cultural pattern of nursing organizations was classified into three patterns which were conservative-dominant culture, symmetric culture and innovative-task culture. Conclusions: The meta culture of the nursing organizations was the conservative culture. But conservative culture decreased nurses' outcomes. Therefore the nurse manager should develop a symmetric organizational culture including four types of culture than dominant culture of one type.

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The avant-garde expressive characteristics of Rei Kawakubo's Collection - A focus on Rei Kawakubo/Comme des Garcons: Art of the in-between - (Rei Kawakubo Collection에 나타난 아방가르드 표현 특성 - Rei Kawakubo/Comme des Garcons: Art of the in-between를 중심으로 -)

  • Shin, Haram;Yum, Misun
    • The Research Journal of the Costume Culture
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    • v.25 no.6
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    • pp.787-803
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    • 2017
  • The purpose of this study is to reestablish the innovative and experimental designs of Rei Kawakubo by considering and analyzing the flow on the avant-garde nature of her design collection from 1981 to 2017. Design trends such as the art trend, silhouette, color, and expressive technique showcased in Rei Kawakubo's collection from 1981 spring/summer to 2017 spring/summer were examined through precedent studies, books, internet materials, and the avant-garde expressive nature of her designs appearing in modern fashion. Additionally, this study considers the definition of avant- garde and analyzes the expressive nature of Rei Kawakubo's 137 works from the Comme des Garcons collection, exhibited in New York's Metropolitan Museum of Art. As a result of this examination, the avant-garde expressive nature seen in modern fashion was classified into the following themes: historicity, de-structure, exaggeration, intermixture, and surrealism. As a result of reclassifying these characteristics according to historical flow, the historicty, intermixture, and de-structure appeared in the 1980s, while intermixture, exaggeration, and de-structure appeared in the 1990s. More recently, historicity, intermixture, and de-structure appeared in the 2000s, and intermixture, de-structure, exaggeration, and surrealism appeared in the 2010s. The present study is significant in providing theoretical material for the more innovative and various design development in diverse domains while helping to define and understand the avant-garde expression through Rei Kawakubo's collection.

Research of Data Collection for AI Education Using Physical Computing Tools (피지컬 교구를 이용한 인공지능 교육용 데이터 수집 연구)

  • Lee, Jaeho;Jun, Doyeon
    • Journal of Creative Information Culture
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    • v.7 no.4
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    • pp.265-277
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    • 2021
  • Data is the core of AI technology. With the development of technology, AI technology is also accelerating as the amount of data increases explosively than before. However, compared to the interest in AI education, research on data education with AI is still insufficient. According to the case analysis of exsisting AI data education, there were cases of educating the process and part of data science, but it was hard to find studies related to data collection. Cause physical computing tools have a positive effect on AI education for elementary school students, data collection cases using tools were studied, but researches related to data collection were rare. Therefore, in this study, an efficient data collection method using physical tools was designed. A structural diagram of a data collection program was created using COBL S, a modular physical computing teaching tool, and examples of program screens from the service side and the user side were configured. This study has limitations in that the establishment of an AI education platform that can be used in conjunction with future program production and programs should be prioritized as a proposal in terms of design.

The Latin American Culture in Fashion Designs in 2000's (2000년 이후 패션 디자인에 나타난 라틴 아메리카 문화)

  • Choi, Ho-Jeong
    • Journal of the Korean Society of Costume
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    • v.57 no.4 s.113
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    • pp.159-172
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    • 2007
  • For the study on the Latin American culture reflected in fashion designs since 2000, the applications of Latin American culture shown in the four major collections from 2000 S/S to 2005 F/W (110 pieces) and some African designers' collections (157 pieces) have been analyzed and compared in three categories - forms, patterns and accessories. First, in the formal application of traditional clothes, the traditional elements of Indio culture are utilized in both the four major collections (76.8%) and the Latin American designers' collection (77.1%). Quechquemitl, the traditional Indio clothes are utilized in various forms in the four major collections, while the Latin American designers adopt various forms of traditional clothes, such as quechquemitl, camisa and pollera. Second, in the textile design, the patterns from Indio's traditional textile design are utilized in both the four major collections (68.7%) and the Latin American designers's collections (5.6%). The remarkable difference between the Latin American designers and the western designers is that the former like to mix the simple and primitive Indio culture with the colorful Iberian culture, and to utilize various patterns of feather, which is an important symbol in the traditional culture, expressing tradition in the modern touch. On the other hand, the western designers change the primitive and handcraft feel of Indio patterns into colorful ones, or mix the colorful Spanish-style flower patterns with primitive and passionate feel. Third, simple and handcraft feel of Indio accessories are utilized in modern fashion in both the four major collections and the Latin American designers' collections. The most remarkable difference between the two group of designers' collections is that various feather patterns are used in Latin American designers' collection, while the accessories reminding of relics of Maya and Inca are widely used in the four major collections.

현대 남성 패션에 표현된 문화 꼴라쥬 현상

  • 이민선
    • Proceedings of the Costume Culture Conference
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    • 2004.04a
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    • pp.92-94
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    • 2004
  • 본 연구에서는 이상 제기한 연구 문제를 해결하기 위해서 미학 및 사회학 등에서 논의되어 온 문화 꼴라쥬 현상에 대한 개념과 사회적 등장 배경 등에 대한 이해를 바탕으로 문화 꼴라쥬 현상에 대한 내적 가치를 파악하고자 한다. 남성 패션에 표현된 문화 꼴라쥬 현상의 외적 조형성을 분석하기 위해서는 시각적 자료로 남성 패션 스타일 선도자로서의 역할을 해온 The Face, i-D, view와 해외 collection 지 등의 잡지를 분석한다. (중략)

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The Plan for Activating Collection Services of Public Library in Seoul Metropolitan (서울시 공공도서관 자료서비스 활성화 방안)

  • Yoon, Hee-Yoon
    • Journal of Korean Library and Information Science Society
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    • v.45 no.1
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    • pp.5-25
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    • 2014
  • The goal of this study is to propose the plan for activating collection services of public library in Seoul. For this goal, author analyzed and compared the core infrastructure, collection services, and their correlation of public libraries in 16 local governments and evaluated environment and the current situation of collection service of public libraries in Seoul. Based on the these results, author suggested five plans or strategies for vitalizing the collection services of public libraries. All public libraries in Seoul must maximize the visibility of the new collections, supply the culture(life-long learning) programs based on library collections, expand the breadth of the collection service through library cooperation system, strengthen interlibrary loan by establishment of the collaborative repository, and raise the awareness of the citizens about the library and collections.