• 제목/요약/키워드: Cultivation Analysis

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Mcl-PHAs Produced by Pseudomonas sp. Gl01 Using Fed-Batch Cultivation with Waste Rapeseed Oil as Carbon Source

  • Mozejko, Justyna;Wilke, Andreas;Przybylek, Grzegorz;Ciesielski, Slawomir
    • Journal of Microbiology and Biotechnology
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    • 제22권3호
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    • pp.371-377
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    • 2012
  • The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fed-batch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source. The biomass value reached 3.0 g/l of CDW with 20% of PHAs content within 48 h of cultivation. The polymer was purified from lyophilized cells and analyzed by gas chromatography (GC). The results revealed that the monomeric composition of the obtained polyesters depended on the available substrate. When glucose was used in the growth phase, 3-hydroxyundecanoate and 3-hydroxydodecanoate were found in the polymer composition, whereas in the PHAs-accumulating stage, the Pseudomonas Gl01 strain synthesized mcl-PHAs consisting mainly of 3-hydroxyoctanoate and 3-hydroxydecanoate. The transcriptional analysis using reverse-transcription real-time PCR reaction revealed that the phaC1 gene could be transcribed simultaneously to the phaZ gene.

친환경(親環境) 쌀농업체계(農業體系)로의 전환(轉換)과 식량수급정책(食糧需給政策)의 조정문제(調整問題) (Adoption of Environment-Friendly Rice Farming System and Adjustment of Food Self-Sufficiency Policy)

  • 권용대
    • 농업과학연구
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    • 제28권1호
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    • pp.48-58
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    • 2001
  • This study was conducted to investigate the effects of environmental sound rice farming method on the productivity of rice industry which may result in impacts on the staple food securities. Recently fanners have been concerned about adopting new rice cultivation method such as organic and low input farming system in which fertilizer and pesticide can be substantially reduced so as to alleviate the burden of agro-ecosystem. However, It has been argued about whether or not there are negative impacts on the self sufficiency rate of food, income of farm household and technological adaptability. Therefore this study examined the productivity trend of environmental rice farming system and predicted the long term rice self-sufficiency rate when environmental rice farming system are adopted by assuming various scenarios. It was estimated that rice self-sufficiency rate can be decreased up to 52.2% by 2010. Based on the analysis of results, policy recommendations for environmentally sound rice farming were suggested as follows: 1) gradual adopting and transferring of environmental rice cultivation method, 2) increasing profitability of rice growing fanners 3) developing the farm level technology specific to Korean farming condition, 4) institutionalization of direct payments for encouraging environmental rice fanning.

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Yield, Nutritional Content, and Antioxidant Activity of Pleurotus ostreatus on Corncobs Supplemented with Herb Residues

  • Jin, Zhiqiang;Li, Yunling;Ren, Jiahong;Qin, Nan
    • Mycobiology
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    • 제46권1호
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    • pp.24-32
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    • 2018
  • Improper disposal of herb residues in China has caused severe problems to the surrounding environment and human safety. Three herb residues, i.e., compound Kushen injection residues (CKI) and part one and part two of Qizhitongluo Capsule residues (QC1 and QC2, respectively), were used for the cultivation of Pleurotus ostreatus. The effect of the supplementation of corncobs (CC) with different herb residues on yield, nutritional composition, and antioxidant activity of P. ostreatus was investigated. Compared to the control, the higher mycelial growth rate was observed on substrates CC +30% CKI and CC +30% QC1, while the higher yield was obtained from substrates CC +30% QC2 and CC +30% CKI. Moreover, chemical analysis of fruit bodies revealed that the addition of herb residues to CC significantly increased proteins, amino acids, ashes, minerals (Na and Ca), and total phenolic contents but significantly reduced carbohydrates and $IC_{50}$ values of DPPH radicals. In addition, no heavy metals (Pb, Cd, and As) were detected in the fruiting bodies harvested from different substrate combinations. These results demonstrated that mixtures of CC with herb residues might be utilized as a novel, practical, and easily available substrate for the cultivation of P. ostreatus, which is beneficial for the effective management of herb residues.

Antagonistic Evaluation of Chromobacterium sp. JH7 for Biological Control of Ginseng Root Rot Caused by Cylindrocarpon destructans

  • Han, Joon-Hee;Park, Gi-Chang;Kim, Kyoung Su
    • Mycobiology
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    • 제45권4호
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    • pp.370-378
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    • 2017
  • Cylindrocarpon destructans is an ascomycete soil-borne pathogen that causes ginseng root rot. To identify effective biocontrol agents, we isolated several bacteria from ginseng cultivation soil and evaluated their antifungal activity. Among the isolated bacteria, one isolate (named JH7) was selected for its high antibiotic activity and was further examined for antagonism against fungal pathogens. Strain JH7 was identified as a Chromobacterium sp. using phylogenetic analysis based on 16S rRNA gene sequences. This strain was shown to produce antimicrobial molecules, including chitinases and proteases, but not cellulases. Additionally, the ability of JH7 to produce siderophore and solubilize insoluble phosphate supports its antagonistic and beneficial traits for plant growth. The JH7 strain suppressed the conidiation, conidial germination, and chlamydospore formation of C. destructans. Furthermore, the JH7 strain inhibited other plant pathogenic fungi. Thus, it provides a basis for developing a biocontrol agent for ginseng cultivation.

원격탐사자료와 GIS를 이용한 라오스 남칸유역분지의 토지이용평가 및 미작적지분석 (Land Use Evaluation and Suitablility Analysis for Paddy Cropping of Nam Khane Watershed, Laos, Using Remotely Sensed Data and Geographic Information Systems)

  • 조명희
    • 대한원격탐사학회지
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    • 제11권1호
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    • pp.1-17
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    • 1995
  • 원격탐사자료(MOS-1)를 이용하여 라오스 북부 남칸유역분지의 토지이용형태를 분류하 고, 항공사진과 지형도 및 현지조사에 의한 자료를 기초로 하여 Arc/Info 시스템에서 10종류의 주 제도를작성하였다. 이가운데 취락, 소유역분지, 지형고도, 사면경사를 기준으로하여 토지이용분포 의 특성을 각 주제도면을 중첩 분석하여 규명하였다. 더욱이 화전지역의 분포특성을 파악하고, 휴 경지와 화전지역에 대하여 미작이 가능한 영구농지로 전환할 수 있는 적지를 추출하여 유역관리 를 위한 기초자료를 산출하였다.

양파농가의 공동체의식이 참여만족에 미치는 영향 분석 (The Effect of Sense of Community on Participation Satisfaction in Onion Farms)

  • 이정세;마지영;이남수;백경문;장동헌
    • 농촌계획
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    • 제27권1호
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    • pp.95-102
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    • 2021
  • This study analyzed the effect of the farm's sense of community on participation satisfaction by participating in the contract cultivation of onions. To this end, we investigated the participation satisfaction with the sense of community's emotional connection, fulfillment of need, membership, and influence. According to the analysis of the sense of community of onion farmers, the definition of need, membership, and influenced participation satisfaction and were statistically significant. It was also shown to affect sense of community in the order of membership, Influence, fulfillment of need, and emotional connection. Therefore, it is necessary to form a continuous relationship with the leader and Nonghyup, to lead to improved farm income, induce pride as a member of participating farmers, and form a positive perception among onion farmers.

산초나무 자생지의 입지환경과 생태적 특성 (Site Environment and Ecological Characteristics of the Zanthoxylum schinifolium Habitats)

  • 김소진;김태운;문현식
    • 한국환경과학회지
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    • 제31권7호
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    • pp.665-675
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    • 2022
  • The purpose of this study was to provide basic information for judging cultivation suitability by analyzing the growing environment of Zanthoxylum schinifolium habitats in Gyeongnam-do. The site environment, soil environment and community structure were analyzed in habitats of 7 cities and counties. The habitats were distributed in slopes at 87~764 m above sea level. It was found habitats was distributed in the well-drained soil with high content of sand and soil texture of habitats was mainly loamy sand and sandy loam. Bulk density and particle density were 0.89 g/cm3 and 2.65 g/cm3 on average, respectively. The soil had 5.10 of pH, 6.41% of OM content, 0.29% of TN content, 3.84 ppm of available P content, and CEC of 12.3 cmol+/kg on average. The habitats were classified into four communities (Z. schinifolium-Lindera erythrocarpa, Castanea crenata-L. erythrocarpa, Pinus densiflora-Z. schinifolium, P. thunbergii-Z. schinifolium by clustering analysis. Ecological niche breadth was highest of 0.885 in site (T VI ongyeong) and lowest of 0.608 in site (Goseong).

Cavitation optimization of single-orifice plate using CFD method and neighborhood cultivation genetic algorithm

  • Zhang, Yu;Lai, Jiang;He, Chao;Yang, Shihao
    • Nuclear Engineering and Technology
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    • 제54권5호
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    • pp.1835-1844
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    • 2022
  • Single-orifice plate is wildly utilized in the piping system of the nuclear power plant to throttle and depressurize the fluid of the pipeline. The cavitation induced by the single-orifice plate may cause some serious vibration of the pipeline. This study aims to find the optimal designs of the single-orifice plates that may have weak cavitation possibilities. For this purpose, a new single-orifice plate with a convergent-flat-divergent hole was modeled, a multi-objective optimization method was proposed to optimize the shape of a single-orifice plate, while computational fluid dynamics method was adopted to obtain the fluid physical quantities. The reciprocal cavitation number and the developmental integral were treated as cavitation indexes (e.g., objectives for the optimization algorithm). Two non-dominant designs ultimately achieved illustrated obvious reduction in the cavitation indexes at a Reynolds number Re = 1 ×105 defined based on fluid velocity. Besides, the sensitivity analysis and temperature effects were also performed. The results indicated that the convergent angle of the single-orifice plate dominants the cavitation behavior globally. The optimal designs of single-orifice plates result in lower downstream jet areas and lower upstream pressure. For a constant Reynolds number, the higher temperature of liquid water, the easier it is to undergo cavitation. Whereas there is a diametric phenomenon for a constant fluid velocity. Moreover, the regression models were carried out to establish the mathematical relation between temperature and cavitation indexes.

Application of Internet of Things Based Monitoring System for indoor Ganoderma Lucidum Cultivation

  • Quoc Cuong Nguyen;Hoang Tan Huynh;Tuong So Dao;HyukDong Kwon
    • International journal of advanced smart convergence
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    • 제12권2호
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    • pp.153-158
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    • 2023
  • Most agriculture plantings are based on traditional farming and demand a lot of human work processes. In order to improve the efficiency as well as the productivity of their farms, modern agricultural technology was proven to be better than traditional practices. Internet of Things (IoT) is usually related in modern agriculture which provides the farmer with a real-time monitoring condition of their farm from anywhere and anytime. Therefore, the application of IoT with a sensor to measure and monitors the humidity and the temperature in the mushroom farm that can overcome this problem. This paper proposes an IoT based monitoring system forindoor Ganoderma lucidum cultivation at a minimal cost in terms of hardware resources and practicality. The results show that the data of temperature and humidity are changing depending on the weather and the preliminary experimental results demonstrated that all parameters of the system were optimized and successful to achieve the objective. In addition, the analysis results show that the quality of Ganoderma lucidum produced on the research method conforms to regulations in Vietnam.

Analysis of the International Flowers & Plants Expo Tokyo in 2017

  • Choi, So Young;Park, Hye Min;Lee, Ja Hee;Lee, Ae Kyung
    • 인간식물환경학회지
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    • 제22권1호
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    • pp.91-98
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    • 2019
  • This study aimed to provide basic data on current trends in floriculture through a survey. The exhibitors' purposes and items displayed at the Tokyo International Flowers & Plants Expo in 2017 were surveyed. The survey was conducted among 238 exhibitors during the exhibition period from October 11 to 13, 2017. As a result, participants came from 14 countries including Belgium, China, Colombia, Japan, Korea and France. The purposes of the majority of the surveyed exhibitors were to display cultivars (86 exhibitors, 36.3%), followed by to display merchandise, subsidiary materials and cultivation techniques. Among cultivars, pot plants accounted for the majority of the entire cultivars (54 exhibitors, 55.1%), followed by cut flowers (35 exhibitors, 35.7%), sapling, and seeds. The most of the displayed pot plants were ornamental plants (27 exhibitors, 43.5%), followed by garden trees, ground cover plants, bedding plants and succulent plants. The most of the displayed cut flowers were rose (8 exhibitors, 21.1%), followed by lisianthus, chrysanthemum and dahila. In terms of cultivation techniques, those using moss were displayed by 7 exhibitors (50%) and green-wall techniques were displayed by 6 exhibitors (42.9%). In terms of merchandise, processed products were displayed by 21 exhibitors (41.2%), followed by floral products. Therefore, the IFEX can be used as a place to understand current trends in the floriculture market.