• Asian-Australasian Journal of Animal Sciences
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• v.17 no.6
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• pp.877-884
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• 2004

Although gut microbial functions have been analyzed through cultivation of isolated microbes, molecular analysis without cultivation is becoming a popular approach in recent years. Gene cloning studies have partially revealed the mechanisms involved in fiber digestion of individual microbe. The molecular approach finally made it possible to analyze full genomes of the representative rumen cellulolytic bacteria Fibrobacter and Ruminococcus. The coming database may contain useful information such as regulation of gene expression relating to fiber digestion. Meanwhile, unculturable bacteria are still poorly characterized, even though they are main constituents of gut microbial ecosystem. The molecular analysis is essential to initiating the studies on these unculturable bacteria. The studies dealing with rumen and large intestine are revealing considerable complexity of the microbial ecosystems with many undescribed bacteria. These bacteria are being highlighted as possibly functional members contributing to feed digestion. Manipulation of gut bacteria and gut ecology for improving animal production is still at challenging stage. Bacteria newly introduced in the rumen, whether they are genetically modified or not, suffer from poor survival. In one of these attempts, Butyrivibrio fibrisolvens expressing a foreign dehalogenase was successfully established in sheep rumen to prevent fluoroacetate poisoning. This expands choice of forages in tropics, since many tropic plants are known to contain the toxic fluoroacetate. This example may promise the possible application of molecular breeding of gut bacteria to the host animals with significance in their health and nutrition. When inoculation strategies for such foreign bacteria are considered, it is obvious that we should have more detailed information of the gut microbial ecology.

• KSBB Journal
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• v.31 no.3
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• pp.158-164
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• 2016

Plant growth promoting (PGP) hormones, which are produced in a small quantity by bacteria, affect in plant growth and development. PGPs play an important role on the crop productivity in agricultural field. In this study, a photosynthetic bacterial strain producing the PGP was isolated from paddy soil. Bacterial isolate was gram negative, rod-shaped and motility positive. From the 16s rRNA gene sequence analysis, the isolate was identified as Rhodobacter capsulatus PS-2. The mass cultivation of R. capsulatus PS-2 was optimized by considering of the carbon, nitrogen and inorganic salt sources. Optimal medium composition was determined as Na-succinate 4.5 g, yeast extract 5 g, $K_2HPO_4$ 1 g, $MgSO_4$ 5 g, per liter. From the result of 500 L fermentation for 2 days using the optimal medium, the viable cells were $8.7{\times}10^9cfu/mL$. R. capsulatus PS-2 strain produced the carotenoid and indole-3-acetic acid (IAA). The carotenoid extraction and quantitative analysis were performed by HCl-assisting method. Total carotenoid contents from R. capsulatus PS-2 culture broth were measured as $7.02{\pm}0.04$ and $6.93{\pm}0.05mg/L$ under photoheterotrophic and chemoheterotrophic conditions, respectively. To measure the productivity of IAA, colorimetric method was employed using Salkowski reagent at optical density 535 nm. The results showed that the highest content of IAA was $197.44{\pm}5.92mg/L$ in the optimal medium supplemented with 0.3% tryptophan.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.274-281
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• 2006

In this study, I aimed to suggest political data on the plantation of chestnut trees given the local features by conducting an analysis on the economic efficiency of the plantation of chestnut trees aimed at chestnut tree plantation farmhouses (total 67 farmhouses) in Chungbuk, Chungnam, Jeonbuk, Jeonnam and Gyeongnam, which are the chief producing place in Korea. As a result, the central area (Chungbuk, Chungnam) was proven to post 2.7 times as many as the average total income, 3.9 times as many as income, and 12.1 times as many as net income per ha when compared with those in the southern area (Jeonnam, Gyeongnam). That is, the southern area demonstrated a very low economic efficiency on the plantation of chestnut trees compared with that of the central area, and this was caused by the reduction of chestnut production and the degradation of chestnut quality due to the aging of chestnut trees. Therefore, it is imperative to consistently renovate aged chestnut orchards and manage them in the southern area.

• Smart Media Journal
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• v.11 no.4
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• pp.56-61
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• 2022

The purpose of this study is to analyze affecting factors on the income, farming scale, and farming implementation of graduates of the Korea National College of Agriculture and Fisheries using panel model. For this, we used a generalized estimation equation among the panel analysis methods. The factors that have a positive (+) effect on income were men, married people, and successive farmers. In the case of parents' cooperative farming, dairy farming or poultry farming, matching the major at the time of graduation with the main items, the income was also high. Factors that have a positive (+) effect on farming scale were unmarried people, parents' cooperative farming, aquaculture cultivation, and poultry farming. The factors that implemented the mandatory farming implementation well were men, married people, parents' cooperative farming, aquaculture cultivation, and pig farming. Through the results of this study, it will be possible to help manage and support graduates and enrolled students.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.6
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• pp.430-438
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• 2009

An attempt was made to establish the suitability classes of lands for the cultivation of ginseng(Panax ginseng C. A. Meyer). For this study, the relationships between various soil characteristics and ginseng yields were investigated on altogether 450 ginseng fields (150 sites in paddy and 300 sites in upland), across Kangwon, Kyunggi, Chungbug, Chungnam, Jonbug and Kyungbug Provinces, where ginseng is widely cultivated. In the paddy fields, most influential properties of soil on the ginseng yields was found to be the drainage class. Texture of surface soil and available soil depths affected the ginseng yields to some extents. However, the topography, slope, and the gravel content were found not to affect the ginseng yields. In the uplands, the texture of surface soil was most influential and the topography, slope, and occurrence depth of hard-pan were least influential on the performance of the crop. Making use of multiple regression, by SAS, the contribution of soil morphological and physical properties such as, topography, surface soil texture, drainage class, slope, available soil depth, gravel content, and appearance depth of hard-pan, for the suitability of land for ginseng cultivation was analyzed. Based on the results of above analysis, adding up all of the suitability indices, land suitability classes for ginseng cultivation were proposed. On top of this, taking the weather conditions into consideration, suitability of land for ginseng cultivation was established in paddy field and in uplands. As an example, maps showing the distribution of suitable land for ginseng cultivation were drawn, adopting the land suitability classes obtained through current study, soil map, climate map, and GIS information, for Eumsung County, Chungbug Province. Making use of the information on the land suitability for ginseng cultivation obtained from current study, the suitability of lands currently under cultivation of ginseng was investigated. The results indicate that 74.0% of them in paddy field and 88.3% in upland are "highly suitable" and "suitable".

• Research in Plant Disease
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• v.22 no.4
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• pp.264-268
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• 2016

The nursery test against rice blast in Korea from 2014 to 2015 was analyzed. The average of disease severity of leaf blast in 12 sites showed $3.7{\pm}2.1$ in 2014 and $4.4{\pm}2.1$ in 2015. Disease severity of leaf blast in Icheon and Cheolwon was increased ranging from $2.8{\pm}2.2$ in 2014 to $6.3{\pm}1.8$ in 2015. Using a designation system, a total of 588 isolates collected those years were categorized into 34 races in 2014 and 51 races in 2015 based on the reaction pattern of Korean differential varieties. The blast isolates of 2015 were more diverse than those in 2014. The ratios of KI race to KJ race were 54:46 in 2014 and 70:30 in 2015; however, the predominant race population was KJ-301 as 16%, and KI-101 as 15% in 2014 and 2015, respectively. These results indicate that the distribution of the blast races is getting more diverse in Korea, therefore, this research would provide the possibility to predict race distribution and change to prevent the outbreak of rice blast and will also serve as a useful information for breeding of resistant rice cultivar against blast.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.125-137
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• 2006

Pleurotus eryngii is one of the most promising mushrooms produced on the domestic farms. The quality as well as quantity of Eryngii is sensitively affected by micro climate factors such as temperature, relative humidity, $CO_2$ concentration, and light intensity. To safely produce high-quality Eryngii all the yew round, it is required that the environmental factors be carefully controlled by well designed structures equipped with various facilities and control systems. At the commercial mushroom cultivation houses of permanent frame type (A, B), this study was carried out to find out reasonable range of each environmental factor and yield together with economic and safe structures influencing on the optimal productivity of Eryngii. This experiment was conducted for about two-year ken Nov. 2003 to Dec. 2005 in cultivation house. Ambient temperature during the experiment period was not predominantly different from that of a normal year. The capacity of the hot water boiler and the piping systems were not enough. Because the capacity of electric heater and air circulation were not enough, air temperatures in cultivation house before improvement of system were maintained somewhat lower than setting temperature, and maximum air temperature difference between the upper and lower growth stage during a heating time period was about 5.1. But the air temperatures after system improvement were maintained within the limits range of setting temperature without happening stagnant of air. Air temperature distribution was generally distributed uniform. Relative humidity in cultivation house before , improvement was widely ranged about $44{\sim}100%$. But as the relative humidity after improvement was ranged approximately $80{\sim}100%$, it was maintained within the range of relative humidity recommended. And $CO_2$ concentration was maintained about $400{\sim}3,300mg{\cdot}L^{-1}$ range. The illuminance in cultivation house was widely distributed in accordance with position, and it was maintained lower than the recommended illuminance range $100{\sim}200lx$. The acidity of midium was some lower range than the recommend acidity range of pH $5.5{\sim}6.5$. The yield was relatively ununiform. In case of bottle capacity of 1,300cc, the mushroom of the lowest grade was less than 3%. The consumption electric energy was quite different according to the cultivation season. The electric energy consumed during heating season was much more than that of cooling season.

• The Journal of Korean Philosophical History
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• no.31
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• pp.33-52
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• 2011

This study intends to understand Kobong's thought, especially the problem of Self-cultivation. Kobong, along with Toegye(退溪), is a major figure to understand Confucian ideas of Chosun in the 16th century. There has been a lot of research centered on Kobong's Four-beginnings(四端) and Seven-emotions(七情), but not much on the Self-cultivation of Kobong. Confucianism is basically to seek after actualization of Perfect Virtue(仁) and the way to be a sage, through the pursuit of self-discipline(修己 明明德) and social practice(安人 新民). The problems of Confucianism might be summarized as follows: interest and appreciation for the source of existence(知天/事天); harmony in relationships and practices(愛人/愛物); both of the above together. Therefore, Self-cultivation is to change the self, the subject of one's life, through the relationship between man and heaven. Kobong and Toegye had debated for about eight years(1559-1566) over the problem of human nature, especially emotion(情), and virtue and vice(善惡) fundamental position of Toegye is that the difference between Four-beginnings(四端) and Seven-emotions(七情) can be understood as emotion with qualitative distinction. By contrast, Kobong sees the relationship between Four-beginnings(四端) and Seven-emotions(七情) as that of total and partial. Discussion on the Four-beginnings(四端) and Seven-emotions(七情) is not restricted within the problem of logical analysis of concepts or theoretical validity, but come to a conclusion with the problem of Self-cultivation(修養). In this sense, Kobong tried to follow Neo-Confucian theory of human nature and self-cultivation, on the assumption of Confucian self-discipline and social practice.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.476-484
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• 2022

This study was conducted to investigate the effect of closed cultivation and open cultivation method and substrate type on the nutrient ion change pattern and growth of sweet pepper (Capsicum annuum L.) 'Scirocco' according to the reuse of drainage in hydroponics. The sowing, transplanting, and application of the closed and open cultivation method were carried out on August 19 and September 16, and October 21, 2021, respectively. As a result of the analysis of nutrients in the drainage, Na⁺ and Cl^- are representative ions that crops do not absorb properly, and as the growth progresses, they are accumulated in the closed method. In addition, since the content of NH₄-N in the drainage is significantly lower than that of NO₃-N, it is thought that NH₄-N is preferentially absorbed rather than NO₃-N due to the ion selectivity of sweet pepper. The growth and fruit characteristics of sweet pepper did not differ significantly between treatments according to the drainage reuse and the type of substrate. In conclusion, if you take care of poor fruiting due to the weakening of power after the middle period in hydroponic cultivation of sweet pepper according to the cultivation method of closed and open, and the substrate type of coir and rock wool, the difference between treatments is not large, so the sweet pepper can be produced by selecting the cultivation methods and substrate types suitable for the conditions of grower. However, as interest in environmental pollution has recently increased, it is judged that there is no need to worry about a decrease in quantity or quality, even if a closed cultivation method is adopted under the assumption that pathogen infection due to drainage reuse is well managed. It is expected that if coir is applied instead of rock wool, which causes a problem of disposal, it will further contribute to the reduction of environmental pollution.

• The Korean Journal of Mycology
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• v.46 no.3
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• pp.281-294
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• 2018

Molecular analysis using the internal transcribed spacer region sequences revealed that the strains used in this study, which were formerly identified as Panellus serotinus, are Panellus edullis. After Universal Fungal PCR Fingerprinting (UFPF) analysis, eight strains of P. edulis were divided into two groups. We conducted fundamental research on mycelial growth and sawdust cultivation to understand the cultural characteristics of eight wild P. edulis strains collected from Korean forests. All strains showed faster and denser mycelial growth on potato dextrose agar (PDA) than on other media (malt extract agar, Sabouraud dextrose agar). Optimal conditions for mycelial growth were: $20^{\circ}C$ on PDA, $25^{\circ}C$ on potato dextrose broth (PDB), and pH 5~8 on PDB at $25^{\circ}C$. Two strains (NIFoS 2407, 3993) were selected as excellent strains based on mycelial growth and density on PDA. NIFoS 2792 showed high cellulase activities on carboxymethyl cellulose (CMC) agar, and NIFoS 2387 and 2804 exhibited high laccase activities on ABTS-containing agar media. The mycelial growth of P. edulis was the fastest on Quercus acutissima and Q. mongolica sawdust media, and mycelial density was the highest on Quercus spp. sawdust-containing media. Sawdust cultivation of P. edulis was successful. The conditions were 80~85 days of cultivation period after spawn inoculation, 10~11 days for primordial formation at $17{\sim}18^{\circ}C$, and 15~20 days for fruiting growth. NIFoS 2804 and 3993 were selected as good strains in terms of cultivation period and mushroom production. These results could be useful for the artificial cultivation of P. edulis.