• Title/Summary/Keyword: Cross-culture

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Clinical And Microbiological Study On The Effect Of Magnoliae cortex And Ginkgo Biloba Extracts Containing Dentifrice In Gingivitis (후박 및 은행물 추출물을 함유한 치약의 임상 및 미생물학적 효과에 관한 연구)

  • Kim, Tae-Il;Yeom, Hye-Ri;Ryu, In-Chul;Bae, Ki-Hwan;Chung, Chong-Pyoung
    • Journal of Periodontal and Implant Science
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    • v.26 no.2
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    • pp.542-556
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    • 1996
  • Previous studies have shown that Magnoliae cortex and Ginkgo biloba extracts were showed on the antimicrobial and anti-inflammatory action, in vitro. The purpose of this study was to evaluate on the effect of antimicrobial and anti-inflammatory activity of Magnoliae cortex and Ginkgo biloba extracts containing dentifrice in gingivitis. 70 subjects with gingivitis were divided into an experimental group which performed normal oral hygiene procedure with Magnoliae cortex and Ginkgo biloba extracts containing dentifrice and a control group which also performed normal oral hygiene procedure with the same dentifrice without the natural extracts and completed a doubleblind, cross-over study. At baseline and 3 weeks, subjects were assayed for clinical study by plaque index, gingival index, pocket depth, GCF rate, and microbiological study by subgingival dental plaque bacterial morphotypes by phase contrast microscopy, total anaerobes, total aerobes, Black pigmented bacteroides, A.actionomycetemcomitans, A.viscosus, C.rectus, Ssenguis; P.gingivalis, P.intennedia by bacterial culture and immunofluorescence microscopy. After 3 weeks using their respective dentifrices, reductions in the clinical indices of subjects were similar between the experimental dentifrice group and a control dentifrice group except for statistically significant much reductions in PI, GI, and GCF rate in the experimental dentifrice group as compared to control dentifrice group. Also statistically significant reductions in the motile rods and Spirochetes were found in both experimental group to compare with control group, however statistically much reduction in total anaerobes, Black pigmented bacteroides, and P.gingivalis, P.intennedia were found in the experimental dentifrice group as compared to control dentifrice group. This results indicates that Magnoliae cortex and Ginkgo biloba extracts containing dentifrice might be useful for elimination of gingival inflammation.

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The Feminism expressed in the Modem Fashion -'The Logic of Sex and Gender' on Feminism- (현대 복식에 나타난 페미니즘 -'성논리'의 연계성을 중심으로-)

  • Shin, Su-Ok;Yang, Sook-Hi
    • Fashion & Textile Research Journal
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    • v.1 no.3
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    • pp.218-230
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    • 1999
  • The purpose of this study is -under the post-modem situation- to emphasize the role of women which has various several logic on feminism, to look forward to division of men's consciousness of women, and to reflect a trend to accept the role of sex which is occurring in the system of sex, that is, 'The Third Gender and Homosexuality'. The category of biological and sociocultural on feminism discussed, meantime expressed the sexual logic under post-modern situation, and probed the sexual logic on feminism which was expressed in modem dress and its ornaments by redefining some characters as follows; Imitation-it was to attain masculinity by imitating the body of the opposite sex. Interaction such situations like...... masculinity in women, feminity in men was demonstrated as transvestism of cross-dressing and the symbolic meaning of homosexuality was demonstrated in the shoes of sociocultural on Feminism. Dispersion appeared as a moderation charm and a resistance against the men-ruling culture. Destruction-expressed the fetishism of punk of porny image and maternal affectional image of biological trend as playful satire. Therefore, It is conceived that Feminism would be created true human-phase come true in the men's world as long as the realization of women themselves make the phase of women higher; and further not only propose new image of women but the system of gender.

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Identification of two cytopathogenic agents, Mycoplasma hyorhinis and mammalian orthoreovirus 3 based on modified particle associated nucleic acids PCR

  • Kim, Hye Kwon;Moon, Hyoung Joon;Park, Seong Jun;Rho, Se Mi;Han, Jae Yeon;Nguyen, Van Giap;Park, Bong Kyun
    • Korean Journal of Veterinary Research
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    • v.51 no.2
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    • pp.129-137
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    • 2011
  • Swine diseases could be caused by unrecognized or minor pathogens. In this study, two unknown cytopathogenic agents were isolated from swine, through cell culture. In order to identify these two cytopathogenic agent (designated CP129 and #2045-7), a particle associated nucleic acids PCR (PANPCR) from previous paper was used with simple modification. The cloning procedure was more specified in this study by adding cell control system. According to the modified PAN-PCR, two and four agentsspecific DNA sequences were obtained from CP129 and #2045-7, respectively, and they were identified as Mycoplasma (M.) hyorhinis and Mammalian orthoreovirus by nucleotide BLAST. Since M. hyorhinis (CP129) was filterable and non-visible by microscope, this unusual virus-like nature of M. hyorhinis (CP129) was discussed. Especially, the reovirus (#2045-7) was a serotype 3 and a triple reassortant among three serotypes of reoviruses. It was grouped with recently reported reoviruses from disease cases (swine, human and feline), based on the genetic analysis of L1 and S1 partial sequences. In conclusion, two unknown cytopathogenic agents were successfully identified using modified PAN-PCR with cell control system and they were characterized in this study.

Factors Associated with Vancomycin-Resistant Enterococcus Colonization in Patients Transferred to Emergency Departments in Korea

  • Kim, Hyun Soon;Kim, Dae Hee;Yoon, Hai-jeon;Lee, Woon Jeong;Woo, Seon Hee;Choi, Seung Pill
    • Journal of Korean Medical Science
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    • v.33 no.48
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    • pp.295.1-295.7
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    • 2018
  • Background: Vancomycin-resistant enterococci (VRE) infections have become a major healthcare-associated pathogen problem worldwide. Nosocomial VRE infections could be effectively controlled by screening patients at high risk of harboring VRE and thereby lowering the influx of VRE into healthcare centers. In this study, we evaluated factors associated with VRE colonization in patients transferred to emergency departments, to detect patients at risk for VRE carriage. Methods: This study was conducted in the emergency department of a medical college-affiliated hospital in Korea. Every patient transferred to the emergency department and admitted to the hospital from January to December 2016 was screened for VRE using rectal cultures. In this cross-sectional study, the dependent variable was VRE colonization and the independent variables were demographic and clinical factors of the patients and factors related to the transferring hospital. Patients were divided into two groups, VRE and non-VRE, and previously collected patient data were analyzed. Then we performed logistic regression analyses of characteristics that differed significantly between groups. Results: Out of 650 patients, 106 (16.3%) had positive VRE culture results. Significant variables in the logistic analysis were transfer from geriatric long-term care hospital (adjusted odds ration [aOR]: 8.017; 95% confidence interval [CI]: 1.378-46.651), hospital days (4-7 days; aOR: 7.246; 95% CI: 3.229-16.261), duration of antimicrobial exposure (1-3 days; aOR: 1.976; 95% CI: 1.137-3.436), and age (aOR: 1.025; 95% CI: 1.007-1.043). Conclusion: VRE colonization in patients transferred to the emergency department is associated primarily with factors related to the transferred hospitals rather than demographic and clinical characteristics.

A New Spray Chrysanthemum Cultivar 'Yes Arang' with Single Type and Yellow Color (황색 홑꽃형 스프레이국화 'Yes Arang' 육성)

  • Pak, Ha-Seung;Choi, Taeg-Yong;Won, Mi-Kyoung;Choi, Won-Chun;An, Jong-Beom;In, Min-Sik
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.1
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    • pp.48-51
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    • 2009
  • A new spray chrysanthemum(Dendranthema grandiflorum Ramat.) cultivar 'Yes Arang' was originated from a cross between 'NOA' and 'SP03-05-04'. This cultivar was tested on the characteristics from 2005 to 2007 for the evaluation and selection including shading and retarding culture. The natural flowering time of 'Yes Arang' is late October. This cultivar was yellow in color, single in flower type for the spray type cut-flower. The diameters of flower and flower center were 4.3 cm and 1.2 cm, respectively. Year round production was possible by shading or lighting treatment. Its number of flowers was 15.1 per plant. In natural and forcing cultivation, the days to flowering was 7 weeks after short day treatment. It has resistance to white rust and the vase life was about 17.2 days in autumn season.

Breeding of a New Asiatic Lily 'Onnuri' for Cut and Pot Flower (절화 및 분화용 아시아틱 나리 '온누리' 육성)

  • Jin, Sung-Yong;Kim, Hee-Jun;Choi, So-Ra;Lee, Jin-Jae;Cho, Hong-Ki;Jang, Ik;Kim, Jeong-Man;Jeong, Jong-Seong
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.3
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    • pp.207-210
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    • 2010
  • An Asiatic lily (Lilium spp.) cultivar 'Onnuri' for cut and pot flower was developed in 2006 at Jeollabukdo Agricultural Research & Extension Services, Iksan, Korea. The cross was made in 1999 between 'Avignon', orange colored, and 'Nove Cento', yellow colored. A line was preliminarily selected as 'A99-6-48' by reason of stable flower color and shape among germinated seedlings. It had been multiplicated through in vitro culture and then bulbed on field during 2002~2003. After characteristics were investigated from 2004 to 2005, finally it was named as an 'Onnuri' in 2006. Flower of 'Onnuri' belongs to orange-red group with its center shown orange group. Plant height is 92.8 cm, flower size is medium as 13.8 cm. Stigma color is red-brown and blooming direction is upward-facing. Flowering time is mid-June.

Effect of low intensity pulsed ultrasound in activating the mitogen-activated protein kinase signaling pathway and inhibition inflammation cytokine synthesis in chondrocytes

  • Kim, Eun-Jung;Kim, Gye-Yeop
    • Physical Therapy Rehabilitation Science
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    • v.3 no.1
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    • pp.33-37
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    • 2014
  • Objective: Low intensity pulsed ultrasound (LIPUS) has been shown to accelerate cell proliferation and tissue healing in both animal models and clinical trials. However, details of the clinical effects of LIPUS have not been well characterized. The aim of this study was to investigate the effect of LIPUS on mitogen-activated protein kinase (MAPK) activation in rat articular chondrocytes. Design: Cross-sectional study. Methods: Chondrocyte were cultured in six well cell culture plates for 72 hours at $37^{\circ}C$ with 5% $CO_2$, and then exposed to LIPUS at 1.5 MHz frequency and $30-mW/cm^2$ power. Changes in chondrocyte activities were evaluated in response to oxydative stress in dose-dependent (0 and 300 uM) and time-dependent (0-24 hr) manner. The cell viability were analyzed using MTT [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide]. The expression of p38 MAPK was measured using western blotting. Results: Oxidative stress was induced in rat chondrocytes using hydrogen peroxide ($H_2O_2$). The cell viability was decreased in chondrocytes after the $H_2O_2$ dose and time-dependent treatment. The p38 MAPK phosphorylation occurred at a significantly increased rate after $H_2O_2$ treated (p<0.05). Expression of p38 MAPK was decreased in the p38 inhibitor groups compared with the oxidative stress-induced chondrocyte damage via the p38 MAPK signaling pathways (p<0.05). Conclusions: It could be concluded that LIPUS can inhibit oxidative stress-induced chondrocyte damage via the p38 MAPK signaling pathways.

3D-culture models as drug-testing platforms in canine lymphoma and their cross talk with lymph node-derived stromal cells

  • An, Ju-Hyun;Song, Woo-Jin;Li, Qiang;Bhang, Dong-Ha;Youn, Hwa-Young
    • Journal of Veterinary Science
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    • v.22 no.3
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    • pp.25.1-25.16
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    • 2021
  • Background: Malignant lymphoma is the most common hematopoietic malignancy in dogs, and relapse is frequently seen despite aggressive initial treatment. In order for the treatment of these recurrent lymphomas in dogs to be effective, it is important to choose a personalized and sensitive anticancer agent. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key characteristics of the original tumor. Objectives: In this study, we established a model of hybrid tumor/stromal spheroids and investigated the association between canine lymphoma cell line (GL-1) and canine lymph node (LN)-derived stromal cells (SCs). Methods: A hybrid spheroid model consisting of GL-1 cells and LN-derived SC was created using ultra low attachment plate. The relationship between SCs and tumor cells (TCs) was investigated using a coculture system. Results: TCs cocultured with SCs were found to have significantly upregulated multidrug resistance genes, such as P-qp, MRP1, and BCRP, compared with TC monocultures. Additionally, it was revealed that coculture with SCs reduced doxorubicin-induced apoptosis and G2/M cell cycle arrest of GL-1 cells. Conclusions: SCs upregulated multidrug resistance genes in TCs and influenced apoptosis and the cell cycle of TCs in the presence of anticancer drugs. This study revealed that understanding the interaction between the tumor microenvironment and TCs is essential in designing experimental approaches to personalized medicine and to predict the effect of drugs.

The Relationship between the Work Environment and Person-centered Critical Care Nursing for Intensive Care Nurses (중환자실 간호사의 근무환경과 인간중심 간호의 관계)

  • Kang, Jiyeon;Lim, Yun Mi
    • Journal of Korean Critical Care Nursing
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    • v.12 no.2
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    • pp.73-84
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    • 2019
  • Purpose : The purpose of this study was to investigate the effect of the perceived nursing work environment (NWE) on person-centered critical care nursing (PCCN) practices among nurses in intensive care units (ICUs). Methods : A cross-sectional research design was adopted for this study; participants were 126 ICU nurses working in five hospitals in B city. From December 2018 to February 2019, a survey questionnaire on NWE and PCCN were conducted. The collected data were analyzed with a multiple hierarchical regression analysis. Results : The mean score for NWE was $2.98{\pm}0.39$. Among the factors, basic work system was the highest ($3.39{\pm}0.41$), followed by leadership of the head nurse ($3.31{\pm}0.55$), interpersonal relationships ($2.77{\pm}0.62$), and institutional support ($2.58{\pm}0.52$). The mean score for PCCN was $3.52{\pm}0.46$. Among the factors, comfort was the highest ($3.77{\pm}0.62$), followed by respect ($3.55{\pm}0.56$), compassion ($3.43{\pm}0.60$), and individuality ($3.38{\pm}0.56$). The interpersonal relationship (${\beta}=.31$, p=.001) and the number of patients per shift (${\beta}=-.23$, p=.005) had a significant effect on PCCN, accounting for 25.9% of the total variance. Conclusion : These results suggest that it is necessary to enhance the relationship-oriented culture and reduce the workload of nurses in order to improve the quality of nursing care in ICUs.

A Cross-sectional Study of Cancer Knowledge and Beliefs Among Faith-based Chinese in the USA Versus Taiwan

  • Hou, Su-I;Cao, Xian
    • Journal of Preventive Medicine and Public Health
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    • v.52 no.1
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    • pp.60-65
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    • 2019
  • Objectives: This study examined cancer knowledge, beliefs, and practice among faith-based Chinese in the USA versus Taiwan to gain better understandings on how environment and culture might play a role for tailored cancer education programs. Methods: A self-administered survey included a validated 10-item Cancer Screening Belief Scale (CSBS), an 8-item Cancer Screening Knowledge Test (CSKT), and a 14-item cancer Warning Signs Test (CWST) was administered. Participants were recruited from 9 Chinese churches (5 in the USA and 4 in Taiwan). Results: A total of 372 Chinese participated, 50% lived in the USA and 50% in Taiwan. Mean age was 44.31 (standard deviation, 14.74), 60% males, and majority had college education (85%). Taiwan participants scored higher on both CSKT (6.13 vs. 5.52; p<0.001) and CWST (6.80 vs. 5.38; p<0.001). Although perceived screening benefits and barriers were similar, Taiwan participants endorsed higher on screening norms (11.67 vs. 10.82; p<0.001). Taiwan participants also indicated more doctor recommending cancer screenings (42.1% vs. 29.6%; p=0.015), USA participants were more likely to have had annual health exams (65.4% vs. 48.9%; p=0.002). Regression results showed that those resided in the USA were 2.38 times more likely to report annual health exams. Married status (odds ratio [OR], 2.85), college education (OR, 2.38), doctor's recommendation (OR, 2.87), no family cancer history (OR, 2.47), and those with lower barriers were significant factors on annual health exams. Conclusions: Taiwan participants scored higher on cancer knowledge and screening norms, while more USA participants reported annual health exams. Taiwan's universal healthcare might play a role on the different healthcare seeking patterns.