• Research in Plant Disease
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• v.28 no.1
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• pp.32-38
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• 2022

A survey of Barley yellow dwarf virus (BYDV) was conducted in major oat-growing areas of Korea in 2020. BYDV is an economically important pathogen of cereal crops that can be transmitted by aphids. The present study evaluated the genetic composition of BYDV in oat from eight geographical areas in Korea. Multiplex reverse transcription-polymerase chain reaction was used to screen 322 oat leaf samples for six BYDV strains (PAV, MAV, SGV, PAS, RPV, and RMV). The 125 samples (~39%) tested positive for BYDV. BYDV-PAV, BYDV-SGV, BYDV-PAS, and BYDV-RPV were detected from oat in different areas. Most of the BYDV-infected samples were assigned to subgroup I (n=112). The results indicate that BYDV-PAV could be dominant throughout Korea. Also, the phylogenetic analysis of coat protein sequences indicated that 23 BYDV isolates from Korea could be separated into two clades, which exhibited high nucleotide sequence similarity. In conclusion, the present survey provides a BYDV infection assessment for domestic oat varieties in Korea and basic information for the development of BYDV control measures in Korea's oat industry.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.276-276
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• 2022

Wheat is one of the most widely grown food crops worldwide. Extreme precipitation and wind disturbances increased due to the abnormal climate, which resulted in increased lodging. Introduction of dwarf genes in wheat significantly increased lodging resistance and productivity in wheat breeding. In this study, we performed the genotyping of dwarfing genes between 'Keumkang' and 'Komac 5' ('Keumkang' mutant). In addition, we investigated the relationship between plant height and several phenotypic characters using F₂ segregation populations derived from crosses between the two varieties. There was no significant difference in phenotypic characters between the two varieties except for plant height. In the genotyping analysis using dwarfing genes, mutations of two dwarfing gene were found to be induced between the two varieties. The four genotypes of the F₂ populations from a crossing between 'Keumkang' and 'Komac 5' were used to compare and evaluate the effects of two dwarfing genes. Plants with two single mutant dwarfing gene and double mutant dwarfing gene revealed reduced plant heights than control plants by 4.5%, 6.9%, and 33.2%, respectively. The phenotype analysis showed that double mutant dwarfing gene affected wheat stem growth as the length decreases from the second node, resulting in decreased plant height. However, there were no significant differences in the agronomic traits between mutant plants and control plant. These results may provide novel information about the effect of double mutant dwarfing gene on plant height, and may help improve lodging tolerance and wheat yield.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.47-47
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• 2020

In this study, we aimed to study carnation italian ringspot virus (CIRV) in Erigeron annuus (L.) Pers. in Bonghwa County, Korea. The collected samples showed mosaic and malformation symptoms. To identify the virus species, we performed high-throughput sequencing, reverse transcription polymerase chain reaction, and cloning. The virus was confirmed to be an unreported species, and therefore we performed genome sequencing of the samples. The complete genome was 4,746 nucleotides in length. The CIRV contained five open reading frames (ORFs), and it showed the typical features of members of the genus Tombusvirus. Phylogenetic analyses revealed that ClRV isolates had the highest nucleotide identities with the CZ isolate (95.89%) from Korea. In recent years, these viruses have sporadically been reported in floral scent and medicinal plants. This research found the first natural host infected with CIRV, and provides baseline information to determine the correlation between weeds and crops.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.6_2
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• pp.1073-1082
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• 2023

Thermal sensors, also called thermal infrared wavelength sensors, measure temperature based on the intensity of infrared signals that reach the sensor. The infrared signals recognized by the sensor include infrared wavelength(0.7~3.0㎛) and radiant infrared wavelength(3.0~100㎛). Infrared(IR) wavelengths are divided into five bands: near infrared(NIR), shortwave infrared(SWIR), midwave infrared(MWIR), longwave infrared(LWIR), and far infrared(FIR). Most thermal sensors use the LWIR to capture images. Thermal sensors measure the temperature of the target in a non-contact manner, and the data can be affected by the sensor's viewing angle between the target and the sensor, the amount of atmospheric water vapor (humidity), air temperature, and ground conditions. In this study, the characteristics of three thermal imaging sensor models that are widely used for observation using unmanned aerial vehicles were evaluated, and the optimal application field was determined.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.205-217
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• 2024

Brown rot disease, caused by Monilinia spp., poses a significant threat to pome and stone fruit crops globally, resulting in substantial economic losses during pre- and post-harvest stages. Monilinia fructigena, M. laxa, and M. fructicola are identified as the key agents responsible for brown rot disease. In this study, we employed the amplified fragment length polymorphism (AFLP) method to assess the genetic diversity of 86 strains of Monilinia spp. isolated from major stone fruit cultivation regions in South Korea. Specifically, strains were collected from Chungcheong, Gangwon, Gyeonggi, Gyeongsang, and Jeolla provinces (-do). A comparative analysis of strain characteristics, such as isolation locations, host plants, and responses to chemical fungicides, was conducted. AFLP phylogenetic classification using 20 primer pairs revealed the presence of three distinct groups, with strains from Jeolla province consistently forming a separate group at a high frequency. Furthermore, M. fructicola was divided into three groups by the AFLP pattern. Principal coordinate analysis and PERMANOVA were applied to compare strain information, such as origin, host, and fungicide sensitivity, revealing significant partition patterns for AFLP according to geographic origin and host plants. This study represents the utilization of AFLP methodology to investigate the genetic variability among M. fructicola isolates, highlighting the importance of continuous monitoring and management of variations in the brown rot pathogen.

• Journal of Veterinary Science
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• v.25 no.2
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• pp.33.1-33.11
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• 2024

Agricultural production is a major driver of the Philippine economy. Mass production of animal products, such as livestock and poultry farming, is one of the most prominent players in the field. Filipino farmers use veterinary medicinal products (VMPs) when raising agricultural animals to improve animal growth and prevent diseases. Unfortunately, the extensive use of VMPs, particularly antibiotics, has been linked to drug resistance in animals, particularly antibiotics. Antimicrobial gene products produced in animals due to the prolonged use of VMPs can passed on to humans when they consume animal products. This paper reviews information on the use of VMPs in the Philippines, including the regulations, their impact, challenges, and potential recommendations. The Philippines has existing legislation regulating VMP use. Several agencies were tasked to regulate the use of VMPs, such as the Department of Agriculture, the Department of Health, and the Philippine National Action Plan. Unfortunately, there is a challenge to implementing these regulations, which affects consumers. The unregulated use of VMPs influences the transmission of antibiotic residues from animals to crops to humans. This challenge should be addressed, with more focus on stricter regulation.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.115-124
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• 2024

Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jejudo (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry.

• Korean Journal of Organic Agriculture
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• v.32 no.2
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• pp.137-159
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• 2024

Major crop prices have been raised significantly in recent years by COVID-19, the war in Ukraine, and weather-induced reductions in South American soybean production by unfavoured weather in 2022. Rising international crop prices are likely to destabilize food security in South Korea, which is highly dependent on foreign crops. This study analyzed the impact of soybean import price changes on the domestic soybean market and soybean food self-sufficiency rate from 2024 to 2029 using a dynamic partial equilibrium model. According to the scenario analysis results, if the import prices rise by 10% compared to the baseline, the soybean food self-sufficiency rate would increase by 1.33% in 2024, but it is expected to decrease to -0.58% in 2029 due to the continuous decrease in production. The results of this study are expected to be used as valuable information for policy authorities in establishing policies related to improving food self-sufficiency.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.971-978
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• 2022

Fertigation, which has been introduced in agricultural fields since 1990, has been widely practiced in upland fields as well as in plastic film houses as part of the crop production system. In accordance with demands in the agricultural sector, a huge number of scientific studies on fertigation have been conducted worldwide. Moreover, with a combination of advanced technologies such as big-data, machine learning, etc., fertigation is positioned as an indispensable tool to achieve sustainable crop production and to enhance nutrient and water use efficiency. In this review, we focused on providing valuable information in terms of crop production and nutrient/water use efficiency. A variety of fertigation studies have described that enhancement of crop production did not differ relative to conventional method or slightly increased. In contrast, fertigation significantly improved nutrient/water use efficiency, with a reduction in use ranging from 20 to 50%. Water-soluble organic resources such as livestock manure and agricultural byproducts also have been identified as useful resources like chemical fertilizers. Furthermore, the initial irrigation point was generally recommended in a range of -10 - -40 kPa, although the point differed according to the crop and crop growth stage. From this review, we suggest that fertigation, which is closely integrated with advanced technology, could be a leading technology to attain not only food security but also carbon neutrality via improvement of nutrient/water use efficiency.

• Journal of Ecology and Environment
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• v.48 no.3
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• pp.207-213
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• 2024

Spiders play a vital role in agricultural ecosystems by capturing and preying on small insects, thereby controlling the pests around crops. However, without directly collecting the specimen, it is challenging to accurately determine the species of the spider that formed the web and its diet. Spiders dissolve their prey with digestive fluids while consuming; thus, leaving very little residue in their digestive system. This study aimed to identify the spider that formed the web and the prey caught in the web using environmental DNA (eDNA) present in the spider web. For this purpose, eDNA using the mitochondrial cytochrome C oxidase subunit I (COI) gene was extracted from five adjacent spider webs collected from residences near agricultural environments. Based on the genes extracted from spider webs, it was confirmed that the most commonly found gene in all five spider webs was COI of Parasteatoda tepidariorum, and no other spider genes were detected. Among the five spider webs, prey was found in only one web, and in that web, genes of arthropods other than spiders were detected. The genes of the prey found in the spider web were identified to be those of Orthocladius tamarutilus, Tanytarsus tamagotoi, and Yemma exilis. Thus, without directly collecting arthropod specimens from the spider web, it was possible to identify the spider and its prey. This provides crucial information that can help in clearly understanding the predatory activities of spiders in agricultural ecosystems in the future.