• Journal of Marine Life Science
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• v.6 no.2
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• pp.66-72
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• 2021

We investigated the antioxidant and hepatoprotective effects of extracts from the Undaria pinnatifida and Costaria costata against ethanol-induced oxidative damage. The total polyphenol and flavonoid contents were highest in the 70% ethanol extract from Undaria pinnatifida and Costaria costata. Also, the radical scavenging activity of DPPH (IC₅₀ 0.33± 0.21, 0.48±0.47 mg/ml) and ABTS (IC₅₀ 0.34±0.30, 0.47±0.17 mg/ml) in the 70% ethanol extract was higher than that of the hot water and 10% ethanol extracts. To determine the hepatoprotective effects of extracts in ethanol-induced oxidative damage, cell viability was measured using an MTT assay. In the pre-treatment of Undaria pinnatifida and Costaria costata hot water extracts, the concentration-dependent increased the cell viability compared with the ethanol treated cells (73.95%) by 89.91~97.63% and 84.99~90.54%, respectively. The data suggests that 70% ethanol extracts have antioxidant activity and hot water extracts exhibit hepatoprotective effects. Therefore, Undaria pinnatifida and Costaria costata may be considered potential agents for control ethanol-induced liver damage.

• Journal of Life Science
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• v.16 no.6
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• pp.1044-1051
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• 2006

The barren ground is an abnormal phenomenon of coastal ecosystem in which seaweeds, are destroyed and mostly replaced by the coralline algae containing the calcium carbonate components. To restore the seaweed forest, We have exerted an effort in the local areas, Samchuck, Korea, where barren phenomena are profound. Two methods of seaweed forest construction developed in the present study are underwater longline and seed transplantation for the brown seaweed Costaria costata, a fast growing edible seaweed. The sizes of C. costata attached on the underwater longline were $96.7{\pm}2.2mm$ of blade length and $83.6{\pm}7.7g$ of blade weight in April. Thereafter the sizes declined from May. Similar pattern was obtained from in the transplantation method with maxima of $90.4{\pm}15.8mm$ and $70.1{\pm}31.7g$ for blade length and weight, respectively in April. It appeared totality maturation from two methods in May. This maturation time is the same like that of wild C. costata.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.1
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• pp.71-77
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• 2011

The effects of temperature, light, and salinity on the growth of Costaria costata and Undaria pinnatifida juveniles were examined in laboratory cultures. In a cultivation farm, the monthly yield and density were also investigated between December and April for C. costata and between December and March in 2007 and 2008 for U. pinnatifida. The relative growth rates (RGRs) were greater at $20{\sim}60\;{\mu}mol$ photons $m^{-2}s^{-1}$ than at low ($0{\sim}10\;{\mu}mol$ photons $m^{-2}s^{-1}$) and high ($100{\sim}180\;{\mu}mol$ photons $m^{-2}s^{-1}$) irradiance levels. The optimal growth conditions for the two species were $17^{\circ}C$, 35 psu, $60\;{\mu}mol$ photons $m^{-2}s^{-1}$, and a daylength of 12 h, indicating that C. costata and U. pinnatifida have very similar growth responses to temperature, light, and salinity. However, the growth responses of the two species to various environmental factors were different; C. costata grew faster than U. pinnatifida but the latter species grew well at low salinity. The monthly yield of C. costata and U. pinnatifida increased steadily over the study period, and it was maximal in March for both species, but the yield of U. pinnatifida was greater than that of C. costata.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.2
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• pp.190-197
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• 2016

We examined for differences in the growth and fertility of microscopic stages between Costaria costata and Undaria pinnatifida in crossed temperature×irradiance (12, 17, 22℃×20, 60 μmol photons m^–2s^–1) and daylength×irradiance (8, 12, 16, 24 h×20, 60 μmol photons m^–2s^–1) experimental designs. After 13 days in culture, maximum gametophyte growths of the two species were obtained under the following combinations of factors: 17℃×60 μmol photons m^–2s^–1 and 24 h daylength×60 μmol photons m^–2s^–1. C. costata gametophytes produced sporophytes about 6 days earlier than those of U. pinnatifida. Sporophyte densities were highest in the 12 h daylength×60 μmol photons m^–2s^–1 combination after 20 days in C. costata (9.7 plants mm^–2) and 26 days in U. pinnatifida (9.1 plants mm^–2). However, optimal growth conditions for the microscopic sporophytes differed between species: 17℃×60 μmol photons m^–2 s^–1 for C. costata, 12℃×60 μmol photons m^–2 s^–1 for U. pinnatifida. Thus, C. costata gametophytes grew faster and produced sporophytes earlier than those of U. pinnatifida, even though the haploid gametophytes of the two species responded similarly to environmental conditions. Optimal growth temperatures for the microscopic sporophytes also differed between species.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.149-150
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• 2003

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.8
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• pp.1124-1129
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• 2005

Functional activities of low molecular weight substances purified from pepsin hydrolysates of four different seaweeds; Costaria costata, Enteromorpha prolifera, Grateloupia filicina and Porphyra tenera, were inves-tigated. Each pepsin hydrolysate of Costaria costata, Enteromorpha prolifera, and Grateloupia filicina resulted in three peptide peaks on Bio-Rad P2 gel chromatography pattern, while that of Porphyra tenera showed 2 peaks. Peak 1 of Porphyra tenera showed the highest antioxidative activity followed by peak 2 of Porphyra tenera and peak 2 of Costaria costata in order Peak 1 of Porphyra tenera showed the highest ACE inhibitory activity followed by peak 3 and peak 2 of Enteromorpha prolifera in order. Peak 1 and peak 2 of Porphyra tenera, and peak 2 of Enteromorpha prolifera showed the highest antityrosinase activity followed by peak 3 of Enteromorpha prolifera. Peak 1 of Enteromorpha prolifera showed the highest antitumor activity followed by peak 2 of Costaria costata, peak 3 of Enteromorpha prolifera, and peak 3 of Grateloupia filicina in order. Porphyra tenera showed the highest functional activities, which is thought to be due to its high protein content. Structure and amino acid sequence of low molecular weight peptide of Porphyra tenera should be analyzed in the further study.

• Journal of Plant Biology
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• v.26 no.4
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• pp.181-190
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• 1983

Seasonal occurrence of benthic algae and changes of subtidal vegetation were studied for their species composition, diversity and biomass during 1982 and 1983 at several selected sites at Juckdo Island (38$^{\circ}$12'N, 128$^{\circ}$32'E), eastern coast of Korea. Three large brown algae which played a role in change of algal vegetation through their great biomass were investigated with regard to their seasonal growth. Large brown algae such as Undaria pinnatifida, Costaria costata, Laminaria japonica, Agarum cribrosum, Sargassum confusum and S. hornerii constitute the major portion of vegetation in this area throughout the year. Algal vegetation in spring time is characterized by dominance of species U. pinnatifida and C. costata, whereas the summer vegetation by S. confusum and S. hornerii. In autumn large brown algae are shedded and only small algae, such as Chondrus ocellatus and Grateloupia filicina, remain. The vegetation in winter is dominated by the growth of U. pinnatifida and C. costata. Monthly changes in mean length and weight of randomly collected U. pinnatifida, C. costata and S. confusum are as follows; U. pinnatifida occurs from December to June and shows their maximum growth during March (120 cm in length, 201 g/individual in wet weight), its maximum growth rate is 1.4 cm/day, 3.3 g/day in this month. The growth season of C. costata is very similar to U.pinnatifida, but their average maximum length(110 cm) and weight (106 g/ind.) are lower than U. pinnatifida. The greatest growth rate is during March (1.8 cm/day, 2.0g/day). S. confusum is present throughout the year and reaches the maximum growth (102 cm, 63g/ind.) in July. Maximum growth rate (1.5 cm/day, 1.2 g/day) occurs also during this month. U. pinnatifida and C. costata show different months of maximum growth evidently during the two year. This seems to be caused by a considerable damage to the local vegetation followed by heavy storm in February 1983.

• Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.157-162
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• 2012

To analyze nucleotide sequence encoding internal transcribed spacer (ITS) regions specific to the Laminariaceae family, genomic DNA was isolated from six brown algae species distributed along the east coast of Korea. These included three species from the Laminariaceae family (Agarum clathratum Dumortier, Costaria costata [C. Agardh] Saunders, and Saccharina japonica Areschoug) and two species from the Alariaceae family (Undaria pinnatifida [Harvey] Suringer and Ecklonia cava Kjellman), both in the order Laminariales, and one species from the family Sargassaceae in the order Fucales (Sargassum serratifolium). Based on a sequence analysis of ITS-1 and ITS-2 for A. clathratum, C. costata, and E. cava, oligonucleotides were designed from the regions that showed sequence conservation in Laminariaceae. Following polymerase chain reaction using three sets of primers, amplification of ITS-1 and ITS-2 was detected in reactions using genomic DNA isolated from the species belonging to Laminariaceae, but not from the species belonging to the other families. The results indicate that this method can be used for the detection and identification of Laminariaceae species.

• Journal of Plant Biology
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• v.26 no.3
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• pp.119-130
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• 1983

Algal vegetation in the subtidal zone between a small rock output and the Juckdo Island, eastern coast of Korea was investigated at several selected sites. The objectives of the survey were to descirbe the poorly known macroalgae community in this area and correlate the pattern of distribution as well as the change of zonatin with the environmental conditions. The water movement, light intensity and theinclinatin of substrate are considered as the environmental parameters. The upper and mid subtidal zones in sheltered area with less steep rocky surface are dominated by large brown algae Srgassum confusum; in the exposed area are characterised by species of Costaria costata. In the lower subtidal zone, the difference of vegetaton between the sheltered and the exposed areas is not recognized. At this depth the light intensity is an important ecological factor. Six narrow algal zones occur in the sheltered area, whereas two broad belts occur in the exposed area. the biomass value is 4 times greater in the sheltered area than in the exposed area.

• Korean Journal of Plant Resources
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• v.36 no.2
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• pp.107-121
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• 2023

This study analyzed the polyphenol, flavonoid contents, antioxidant activity, anti-aging activity and phenol component contents of Saccharina japonica (SJ), Costaria costata (CC) extracts with hot water, 95% methanol, 95% prethanol for investigating possible utilization of SJ and CC extracts. The result revealed that the SJ and CC methanol extracts showed the highest polyphenol and flavonoid contents, 4.63 mg TAN/g, and 4.19 mg QUE/g respectively. Also, the SJ and CC methanol extracts showed higher antioxidant activity than prethanol and hot water extracts, whereas the ABTS radical scavenging activities were the highest in prethanol extracts (IC₅₀ = 15.4, 10.3 ㎍/µL). In anti-aging activity for evaluating the anti-wrinkle activity and skin whitening activity, the CC methanol extracts had high collagenase inhibitory activity (88.3%), and the SJ prethanol extracts showed higher elastase inhibitory activity (19.0%) compared to other extracts. Then the tyrosinase inhibitory activity was significantly higher in the SJ and CC methanol extracts (41.8, 30.3%, respectively), whereas prethanol extracts were the lowest. To identify the phenol component contents of SJ and CC extracts, 4-hydroxybenzoic acid, naringenin, naringin and nicotinic acid were measured using LC-MS/MS. As a result, the phenol contents were the highest in SJ methanol extract (4-hydroxybenzoic acid), SJ and CC prethanol extract (naringin and naringenin) and CC prethanol extract (nicotinic acid). Lastly, the antioxidant activity of SJ and CC showed high correlations with polyphenol and flavonoid contents (R = -0.946~0.883). These results suggest that prethanol or methanol extracts of SJ and CC have higher antioxidant activities, anti-aging activity and the potential to be used as material for health functional food and cosmetics.