• Title/Summary/Keyword: Cornus walteri Wanger

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Effects of Stem Bark Extracts of Cornus walteri Wanger on the Lipid Lowering, Anti-oxidative Activity and Concentration of Proinflammatory Cytokines in Rat Fed High Fat Diet (모래지엽(毛棶枝葉)(Cornus walteri Wanger) 추출물이 비만 쥐의 지질강하, 항산화효과 및 전염증성 cytokines 농도에 미치는 영향)

  • Park, Won-Hyung;Cha, Yun-Yeop
    • Journal of Korean Medicine Rehabilitation
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    • v.19 no.4
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    • pp.59-78
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    • 2009
  • Objective : This study was designed to examine the effects of stem bark extracts of Cornus walteri Wanger on the lipid lowering, anti-oxidative activity and concentration of proinflammatory cytokines in hyperlipidemic rat. Methods : Male rats weighing $195.21{\pm}5.85g$ fed high fat diet for 8 weeks and 40 rats(above 400 g) were divided into 4 groups. Each groups were divided into a control group and 3 experimental groups. We fed a control group of rats a basal diet and administered normal saline(100 mg/kg, 1 time/1 day) for 4 weeks. And we fed each experimental group of rats Basal diet and administered an extract of Cornus walteri Wanger(100 mg/kg, 200 mg/kg, 300 mg/kg, 1 time/1 day) for 4 weeks. At the end of the experiment, the rats were sacrificed to determine their chemical composition. We measured lipid of plasma and liver, concentration of proinflammatory cytokines, anti-oxidative activity and gene expression. Results : 1. Concentration of plasma free fatty acid, LDL-cholesterol showed a tendency to decrease in Cornus walteri Wanger ext. groups. Concentration of plasma triglyceride, total cholesterol showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group. HDL-cholesterol showed a significantly increment in the 300 mg/kg Cornus walteri Wanger ext. group. 2. Concentration of liver total cholesterol showed a tendence to decrease in Cornus walteri Wanger ext. groups. Concentration of triglyceride liver showed a significantly decrement in all Cornus walteri Wanger ext. group than that of control group. 3. Concentration of plasma and liver TBARS showed a tendence to decrease in Cornus walteri Wanger ext. groups. The values of GSH-Px, SOD and CAT activity showed a significantly increment in the 300 mg/kg Cornus walteri Wanger ext. group than that of control group. 4. The values of plasma AST and ALT activity showed no significantly different in all treatment groups. 5. Concentration of plasma $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ showed a tendency to decrease in the Cornus walteri Wanger ext. groups. However the concentration of IL-10 in the 200 and 300 mg/kg Cornus walteri Wanger ext. groups showed a significantly increment than that of control group. Concentration of liver $IL-1{\beta}$, $TNF-{\alpha}$ and IL-10 showed no significantly difference in all treatment groups. However concentration of IL-6 in the 200 and 300 mg/kg Cornus walteri Wanger ext. groups showed a significantly decrement than that of control group. 6. In the analysis of RT-PCR, gene expression of $TNF-{\alpha}$, Apo-B, Apo-E and leptin in the Cornus walteri Wanger ext. groups showed a lower expression than that of control group. 7. The ratio of $TNF-{\alpha}$, Apo-E and leptin expression per $\beta$-actin expression in the 200 and 300 mg/kg Cornus walteri Wanger ext. showed a significantly decrement than that of control group. The ratio of Apo-B expression per $\beta$-actin expression in the 300 mg/kg Cornus walteri Wanger ext. showed a significantly decrement than that of control group. Conclusions : According to above results, in lowering lipid effect, antioxidative activity and antiinflammatory effect, the Cornus walteri Wanger ext. gives positive effect.

The Protective Effects of Cornus walteri Wanger Leaves against UV Induced Cellular Damage in Human Fibroblast (자외선에 의한 세포손상에 대한 말채나무잎의 보호효과)

  • Park, Hyun-Chul;Jung, Taek Kyu;Yoon, Kyung-Sup
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.1
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    • pp.1-7
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    • 2015
  • Cornus walteri Wanger has been used in folk medicine in Korea. Ultraviolet (UV) irradiation has been known as a major cause of photo damage in skin. In the present study, research on how to cure damaged cells by UVB was conducted using an extract of Cornus walteri Wanger leaves (CWE), which was treated with an enzyme. CWE was applied to human dermal fibroblasts (HDFs) affected by UVB. UVB-irradiated HS68 cells showed increased caspase-3 activity, phosphorylation of p53, ${\gamma}H2AX$, cyclobutane pyrimidine dimers (CPDs) formation, and DNA fragmentation compared with non-irradiated cells. However, all these effects were inhibited by treatment with CWE for 12 h after UVB irradiation. Furthermore, CWE has proved not to cause primary skin irritation through the human patch test. Collectively, these results suggest that CWE could be a new potential candidate as photoprotective agent against UVB-induced cellular damage in HDFs.

Antioxidative Activity of Extract of Cornus walteri Wanger Leaves in Human Dermal Fibroblast Irradiated by UVB (자외선이 조사된 섬유아세포에서 말채나무 잎추출물의 항산화작용)

  • Park, Hyun-Chul;Jung, Taek Kyu;Yoon, Kyung-Sup
    • KSBB Journal
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    • v.29 no.6
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    • pp.432-436
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    • 2014
  • We investigated antioxidative activity of the ethanol extracts of leaves of Cornus walteri Wanger (CWE) by treated enzyme in human dermal fibroblast (HDFs) irradiated by UVB. We examined the in vitro chemical and cellular antioxidant activities of CWE in HDFs. We employed scavenging assay for the 1,1-diphenyl-2,5-picrylhydrazyl (DPPH) radicals and cellular antioxidative activity of CWE, and we was investigated in $H_2O_2$-treated or UVB-irradiated HDFs. The CWE effectively scavenged DPPH radicals ($IC_{50}$ $7.03{\pm}0.4{\mu}g/mL$) when compared to the scavenging activities of L-ascorbic acid ($IC_{50}$ $4.69{\pm}0.3{\mu}g/mL$). CWE reduced UVB-induced cellular damage in HS68 cells by MTT assay and inhibited intracellular ROS generation in dose-dependent manner. In addition, CWE also attenuated the elevated levels of 8-isoprostane resulting from UVB-mediated oxidative stress. Collectively, these results suggest that CWE could be a new potential candidate as antioxidant against UVB-induced oxidative stress in HDFs.

Phenolic Compounds from the Stem Bark of Cornus walteri Wanger (말채나무 수피의 페놀성 화합물)

  • Choi, Woo-Hoi;Park, Woong-Yang;Hwang, Bang-Yeon;Oh, Gap-Jin;Kang, Shin-Jung;Lee, Kyong-Soon;Ro, Jai-Seup
    • Korean Journal of Pharmacognosy
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    • v.29 no.3
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    • pp.217-224
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    • 1998
  • Eight compounds were isolated from the stem bark of Cornus walteri. On the basis of chemical and spectroscopic evidences, the structures of these compounds were identified as gallic acid, (+)-gallocatechin, (+)-catechin, quercetin, quercitrin, rutin, isoquercitrin and hyperoside respectively.

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Studies on Optimum Shading for Seedling Cultivation of Cornus controversa and C. walteri (층층나무와 말채나무 양묘(養苗)시 적정(適正) 차광율(遮光率)에 관한 연구(硏究))

  • Kim, Jong Jin
    • Journal of Korean Society of Forest Science
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    • v.89 no.5
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    • pp.591-597
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    • 2000
  • This studies were carried out to investigate the optimum shading for seedling cultivation of Cornus controversa and C. walteri. The experiment was performed under five different shading scheme such as 100%, 50%, 30%, 10%, and 2% of the natural full sun light intensity for 7 months in the field condition. Two species showed the highest height growth under 50% of relative light intensity, and the highest diameter growth at root collar was observed both under 100% and 50% of relative light intensity. C. controversa seedlings grown under 50% of relative light intensity produced more total biomass than control seedlings, but root biomass was less in the seedlings under 50% of relative light intensity. In case of C. walteri, total biomass production of the seedlings under 50% of relative light intensity was lower than that of the seedlings under full sun light intensity, but leaves and shoot biomass was slightly high under 50% of relative light intensity. Great reductions of height growth and diameter growth at root collar, and biomass production were observed below 30% of relative light intensity in the both species. T/R ratio of the two species was highest under 30% of relative light intensity, and the chlorophyll content of the seedlings tended to be increased by reduction of relative light intensity.

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Antioxidant Activities of Hot Water Extract from Cornus walteri Wanger against Oxidative Stress Induced by tert-Butyl Hydroperoxide in HepG2 Cells (tert-Butyl Hydroperoxide로 산화 스트레스가 유도된 HepG2 세포에서 말채나무 열수추출물의 항산화 활성)

  • Yeon, Seong Ho;Ham, Hyeonmi;Sung, Jeehye;Kim, Younghwa;Namkoong, Seulgi;Jeong, Heon-Sang;Lee, Junsoo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.10
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    • pp.1525-1532
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    • 2013
  • The objective of this study was to investigate the effect of hot water extract from Cornus walteri Wanger (CWE) on tert-butyl hydroperoxide (TBHP)-induced oxidative stress in HepG2 cells. Generation of reactive oxygen species (ROS), concentrations of cellular lipid peroxidation products and reduced glutathione, and antioxidant enzyme activity were used as biomakers of cellular oxidative status. Cells pretreated with CWE (25~200 ${\mu}g/mL$) showed an increased resistance to oxidative stress in a dose-dependent manner, as revealed by a higher percentage of surviving cells compared to control cells. ROS generation induced by TBHP was significantly reduced when cells were pretreated with 200 ${\mu}g/mL$ CWE for 4 h. Pretreatment with CWE (5~50 ${\mu}g/mL$) prevented the decrease in reduced glutathione and the increase in malondialdehyde and ROS evoked by TBHP in HepG2 cells. Finally, CWE pretreatments prevented the significant increase of glutathione peroxidase, catalase, glutathione reductase, and superoxide dismutase activities induced by TBHP. These results show that CWE has significant protective ability against a TBHP-induced oxidative insult and that the modulation of antioxidant enzymes by CWE may have an important antioxidant effect on TBHP-induced oxidative stress in HepG2 cells.

Studies on the Investigation into Original Form of Natural Monument No.404 Orijangnim in Jacheon-ri, Yeongcheon (천연기념물 제404호 영천 자천리 오리장림의 원형 규명)

  • Lim, Won-Hyeon
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.31 no.3
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    • pp.83-94
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    • 2013
  • The purpose of this study is to offer basic data about prototype conservation of the Orijangnim, natural monument No. 404. For this study, March to August 2010, was a literature surveys and field research, and based on this, the Delphi survey of local residents and experts meeting were diagnosed with forest back ground, spatial extent, in the form of forests, flora and vegetation of the surrounding area, so examined closely the original form. Range of the Orijangnim was presumed from Jacheon middle school entrance($N36^{\circ}05^{\prime}59.9^{{\prime}{\prime}}/E128^{\circ}54^{\prime}32.9^{{\prime}{\prime}}$) to Odong of Hwabukmyeon village entrance($N36^{\circ}05^{\prime}31.0^{{\prime}{\prime}}/E128^{\circ}54^{\prime}32.5^{{\prime}{\prime}}$). The Orijangnim in the original form of the existing forest surrounding the village along gohyeon river going back a long S-shaped about 2km is considered. The investigated vascular plants consist of total 198 taxa : 2 forms, 20 varieties, 179 species, 152 Genera, 72 Families. Most of the 60 kinds of large land plants lots of whole plants accounted for 33.3%. A main vegetation of the Orijangnim have been distributed 473 objects of 21 species, and evergreen conifers is 19% to 92 objects, on the other hand, deciduous broadleaf tree is 79% to 373 objects, so the Orijangnim can be maxed forest that deciduous broadleaf tree is dominant. When consider the composition purpose of forest, environment of location, tree size, surrounding flora and vegetation, because an original vegetation of Orijangnim is suggested Zelkova serrata, Salix glandulosa, Celtis sinensis Pers., Quercus variabilis, Quercus acutissima , Sophora japonica L., Cornus walteri Wanger. we must restore around such species of tree. Cedrus deodara (Roxb.) Loudon, Chaenomeles sinensis, Prunus yedoensis Matsum., Ginkgo biloba , Pinus thunbergii Parl., Populus tomentiglandulosa T. Lee is considered to have been planted in the last.