• 한국작물학회지
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• 제52권3호
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• pp.281-288
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• 2007

Photoperiod sensitive genetic male sterile (PGMS) rice is sterile mutant controlled by photoperiod. A PGMS mutant 920S was sterile grown under long-day (LD) photoperiod (14 h light/10 h dark) but fertile grown under short-day (SD) photoperiod (10 h light/14 h dark). Proteome analysis revealed that 12 protein spots were differentially expressed in the spikelets of 920S plants either treated with LD or SD photoperiod. Among these proteins, three proteins including chlorophyll a/b binding protein, vacuolar ATPase ${\beta}-subunit,\;{\alpha}-tubulin$ and an unknown protein were more than three-fold abundant in the spikelet of the SD-treated plants than those of the LD-treated plants. On the other hand, eight proteins including acetyl transferase, 2, 3- biphosphoglycerate, aminopeptidase N, pyruvate decarboxylase, 60S acidic ribosomal protein and three unknown protein spots were more abundant in the spikelets of the LD-treated plants than those of the SD-treated plants. The results suggest that the observed proteins may be involved in sterile or fertile pollen development under LD or SD photoperiod respectively in the PGMS mutant rice.

• 한국수산과학회지
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• 제34권1호
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• pp.13-16
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• 2001

수온과 광주기 조절에 따른 볼락, Sebastes inermis의 혈장내 성steroid hormone 변화를 조사하였다. 월별 GSI의 변화에서 암컷은 대조구의 경우 11월부터 증가하기 시작하여 1월에 최고값이 되었다. 수온과 광주기를 조절한 실험구중 조절에 반응한 개체들 (Tr-r)에서는 대조구 보다 1개월 늦은 2월에 최고값을 나타내었다 그러나 수온과 광주기의 조절에 반응하지 않은 개체들 (Tr-n)은 GSI의 발달이 중단되어 전 실험기간동안 1.2 이하의 낮은 값을 유지하였다. 수컷의 월별 GSI 변화는 대조구와 조절구 사이에서 뚜렷한 차이를 나타내지 않았다. 암컷에서 혈장 E2와 T의 변화는 GSI의 변화와 같이 수온과 광주기를 조절한 실험구가 대조구에 비해 2개월 늦게 최고값에 도달하였다. GSI의 월별 변화에서 차이가 없었던 수컷에서 혈장 11-KT와 T의 월별 변화도 대조구와 조절구에서 큰 차이를 보이지 않았다. 수온과 광주기를 자연상태보다 2개월 늦추어 사육한 결과, 자연상태의 대조구에 비해 암컷의 성숙이 지연되었다. 이것은 수온과 광주기의 자극이 시상하부-뇌하수체-생식소 축을 따라 전달되면서 성 steroid의 분비가 영향을 미쳤기 때문에 난소의 성숙이 지연되었다고 생각된다.

• Reproductive and Developmental Biology
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• 제34권2호
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• pp.89-94
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• 2010

Melatonin is induced by light information through the retina and leads to growth factor activation. Thus, we investigated the effects of melatonin by controlling the photoperiod of growing young rats. Male Sprague-Dawley rats (n=6; 4 weeks old) were divided into two experimental groups: the L/D group (normal photoperiod; light/dark: 12/12 h; lights on at 9:00 a.m.) and the L/L group (light/light: 24 h). Rat body weight and food consumption were measured daily for 8 weeks. After 8 weeks, the rats were anesthetized with a mixture of ketamine (50 mg/kg) and xylazine (10 mg/kg) and sacrificed. Tissue was then collected for RNA isolation (from brain, heart, liver, kidney, adrenal gland, testis, tibia, hind limb muscles). Also, serum was isolated from blood using a centrifugal separation. The L/L group had significantly lower body weight than the L/D group from 4 to 6 weeks (p<0.05). The L/D group had increased tissue mass, compared with the L/L group, but the difference was not statistically significant. The L/D group had a significantly higher melatonin concentration than the L/L group between the hours of midnight and 2:00 a.m (p<0.01). These results indicate that photoperiod length may affect the secretion of melatonin from the pineal gland. Also, the reduction of nocturnal melatonin secretion may retard the development of growing young rats. In future studies, we plan to compare exogenous melatonin administration with endogenous melatonin concentration induced by photoperiod control. Moreover, we will confirm whether the effects seen in pathological animal models can be reversed by controlling the photoperiod.

• 생명과학회지
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• 제31권3호
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• pp.314-320
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• 2021

본 연구는 환경요인이 성숙기 발달단계의 양식산 수컷 참조기(Larimichthys polyactis)의 성성숙에 미치는 영향을 알아보고자 광주기와 수온에 따른 GSI, 정소 발달 및 혈중 성호르몬 변화를 조사하였다. 실험 1 : 광주기를 자연광주기(Natural Photoperiod, NP, 10L:14D~11L:13D), 장일주기(Long Photoperiod, LP, 14L:10D), 단일주기(Short Photoperiod, SP, 10L:14D)로 설정하였고, 수온은 17℃로 설정하여 90일간 실험하였다. 그 결과, GSI는 실험 30일째와 60일째, 혈중 11-KT 농도는 실험 30일째에 LP 실험구에서 다른 실험구보다 유의하게 높게 나타났다. 실험 2 : 수온을 자연수온(Natural temperature, NT, 19.1~15.0℃), 17℃, 21℃, 25℃로 설정하였고, 광주기는 14L:10D로 설정하여 60일간 실험하였다. 그 결과, GSI는 실험 30일째와 60일째, 혈중 11-KT 농도는 실험 60일째에 25℃ 실험구에서 다른 실험구보다 유의하게 낮게 나타났다. 따라서 수컷 참조기는 단일주기(10L:14D)보다 장일주기(14L: 10D)에서 성성숙이 촉진되고, 25℃ 이상의 수온에서는 성성숙이 억제되는 것으로 생각된다. 이러한 결과는 참조기 번식생리학 연구를 위한 기초자료로 유용하게 이용될 것이라 기대된다.

• 한국양식학회지
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• 제3권1호
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• pp.1-11
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• 1990

참돔의 산란조절은 광주기 l1L: 13D, 수온 $12.2^{\circ}C$에서 시작되어 장일조건 14L:10D, 고수온으로 처리한 결과 $16.5^{\circ}C$에서 산란이 일어났다. 사육수온 $17.0^{\circ}C$ 전후로 유지시켰을 때 산란은 56일간 계속되었고, 자연상태보다 약 1개월 조기채난할 수 있었다. 총산란양은 908만 개이었고 1일 산란양은 15만 개이었다. 명주기와 암주기의 시각별 산란은 18$\~$24시에 많았으나 비교적 분산되어 일어났다. 산란중기에 산란된 난들은 부상수정률이 $71.5\%$였고, 난경은 0.95$\~$0.98mm이었으며, 산란양과는 낮은 상관(r=0.49)을 가졌다. 유구수에 따른 부화율은 1개 $85.3\%$, 2개 $76.9\%$, 3개 $74.3\%$, 4개 $76.6\%$, 5개 이상이 $63.9\%$ 로서 유구수가 많을수록 부화율은 감소하였다. 산란된 전체 난의 산란부터 부화까지의 생존율은 $65\%$이었다.

• Fisheries and Aquatic Sciences
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• 제7권1호
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• pp.16-22
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• 2004

Plasma steroid hormone levels in the viviparous rockfish (Sebastes schlegeli) were examined in relation to gonadal histology under controlled photoperiods and water temperatures. To investigate those effects in S. schlegeli the photoperiod was maintained at 15L:9D in June and then it was gradually decreased to 9L: 15D in October. It was then gradually increased to 12L:12D in January, followed by 14L:I0D in February. The water temperature was $19-20^{\circ}C$ in July. From August to October, it was from $18^{\circ}C\;to\;12^{\circ}C$. Then, it was dropped to a low of $19-11^{\circ}C$ in November to December and then gradually increased to $14-15^{\circ}C$ in February. In females, both plasma $estradiol-l7\beta\;$ (E2) and testosterone (T) levels from August to February showed a similar pattern in both the treatment and the control groups. In the treatment group, the peaks of plasma E2 and T were observed in November, and the peaks were closely correlated to histological observations. Oocytes contained many yolk globules (final vitellogenic oocytes), and oocytes at the migratory nucleus stage increased in size. Plasma levels of progesterone did not change much throughout the experimental period. However, in the control group, the peaks of E2, T, and progesterone were observed in February. These results indicate that the controlled photoperiod and water temperature accelerated sexual maturity, corresponding to the advancement of plasma E2 and T peaks by approximately 3 months. In males, plasma T levels showed a similar pattern from August to October in the treatment and control groups, though levels in the treatment group were higher than those in the control group. From histological observations, the treatment group copulated one month earlier.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.215-221
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• 2017

Bitter melon (Momordica charantia, MC) has been used in traditional Korean medicine in treating diabetes. In addition, some reports were emerged, showing the antifertility activities of MC in mammals. We investigated the effects of ethanolic MC extract on the reproductive activity of golden hamsters whose spermatogenetic capacity is controlled by their photoperiods. The animals were divided into 4 groups: long photoperiod (LP) control, short photoperiod (SP) control, and LP animals treated with MC. The animals were orally ingested with low (0.03 g/kg) or high (0.15 g/kg) concentrations of the ethanolic extracts for 8 weeks on the daily basis. The control animals received the vehicle. The animals were then mated with age-matched females, experienced pregnancy. As results, the LP control animals showed active large testes but SP control animals displayed remarkably reduced testes. The animals treated with both concentrations of MC extracts demonstrated large testes, indicating fertile activity as animals in LP. LP control animals had litters as expected, but SP controls had no litters at all. MC extract showed the same results as LP animals in generating offsprings. These results suggest that the MC extract does not change the photoperiodic influence on reproductive activity of male golden hamsters.

• 한국연초학회지
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• 제18권2호
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• pp.132-137
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• 1996

This experiment was conducted to investigate varietal responses to cool temperature (18℃) and short photoperiod (8 hours a day) in a controlled room of phytotron. Five burley tobacco varieties (Nicotiana tabacum L.), Awelius (very early flowering type), Burley 21 and KB 103 (medium), Banket Al and MB 1 (slightly late) and TC 681 (late) were compared in number of leaves per plant and day to flower in different treatment periods (10, 15, 20, 25 and 30 days). The days to flower decreased significantly than the control (in the phytoron with 14hr photoperiods and a minimum temperature 20'c) when the treatment periods were over 15 days for the varieties Aurelius, Burley 21 and KB 103, and 20 days for TC 681, and 30 days for Banket Al, respectively. And also the number of leaves decreased significantly than that of the control when the periods of treatment were over 10 days for Burley 21 and Aurelius, 15 days form 103, and 20 days for TC 681 and Banket Al, and 30 days for MB 1, respectively. The decreasing ratio in the number of leaves per plant under cool temperature and short photoperiod treatments was the lowest in Banket Al and MB 1. Key words : Nicotiana tabacum, cool temperature, short photoperiod.

• 한국연초학회지
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• 제7권1호
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• pp.25-31
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• 1985

For shortening the tobacco breeding cycle, seedlings with 6, 8 and II leaves of 2 flue.cured tobacco varieties, day. neutral type and photoperiod-sensitive type, were grown in controlled-environment room (CER), programmed for temperature of $18^{\circ}C$ and 8.hour day period of 30 klux, for 20, 30 and 40 days. All of plants of day.neutral type variety treated in CER, regardless of seedling stage and duration, flowered earlier than the untreated plants. In the 6.leaf seedlings stage of photoperiod-sensitive type variety, plants treated for 20 and 30 days in CER did not accelerate the flowering. the tobacco plants, treated with low temperature for 20 days at 8.leaf seedling stage, flowered earlier in comparison with. the other treatment.

• 생물환경조절학회지
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• 제17권1호
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• pp.26-31
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• 2008

환경이 조절되는 폐쇄형 식물생장시스템에서 플러그 셀 크기(105공, 162공, 288공)와 일장(8/16, 12/12, 16/8hr, 낮/밤) 처리를 각각 달리하여 20일간 생산된 감자 플러그 묘 '수미'와 대지'의 생육과 수량에 미치는 영향을 알아보고자 실험을 수행하였다. 정식 7주째 '수미'의 플러그 셀 크기와 일장처리는 생육과 상대생장률에 차이를 주었다. '수미'의 주당 괴경 중은 셀 크기와 일장이 증가함에 따라 증가하였다. '대지'의 괴경 중은 105공 셀 크기에서 높았으며, 일장 처리에 차이를 보였다. 90일 재배 후, '수미'의 괴경 중($258.9{\sim}471.9g$/주)은 105공, 162공 처리와 16/8 hr 일장 처리에서 괴경 중이 높았다. '대지'의 괴경중 ($278.2{\sim}428.0g$/주)은 105공 처리에서 높았으나 일장처리 간에는 차이가 없었다. 주당 괴경 수는 '수미'가 $2.9{\sim}6.9개, '대지' $2.2{\sim}3.6개로 플러그 셀 크기와 일장 처리에 따른 유의성은 없었다. 80g 이상의 괴경 크기 비율이 '수미'는 $32{\sim}50.9%, '대지'는 $41.0{\sim}56.7%로, 두 품종 모두 288공 셀 크기에서는 감소하였고, 일장 처리 간에는 품종에 따른 차이를 보여 '수미'는 일장이 증가함에 따라 괴경 수가 증가하는 경향을 보였으나, '대지'는 일장 처리에 따른 차이를 보이지 않았다. 이상의 결과, 초기 묘 소질이 감자 생육 및 괴경 중에 영향을 줌으로써 플러그 묘를 이용한 감자 생산에 적합한 플러그 셀 크기는 162공 이하, 12시간 이상의 일장이 필요하리라 판단되었다.