• Journal of Microbiology
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• v.44 no.1
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• pp.106-112
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• 2006

In this study, the genetic diversities of multi-resistant Salmonella typhimurium (ST) isolates were analyzed via the application of both pulsed field gel electrophoresis (PFGE) and Polymerase chain reaction (PCR) analysis methods, using 6 kinds of primers (REP, ERIC, SERE, BOX, P-1254 and OPB-17). And their discriminative abilities (DA) were also compared in order to determine the most effective and reliable analysis method. 118 S. typhimurium isolates, cultured from diverse animals and human patients in Korea beginning in 1993, were analyzed and subjected to a comparison of Simpson's index of diversity (SID), using both PFGE and PCR methods. PFGE by XbaI enzyme digestion allowed for discrimination into 9 pulsotypes, with high SID values (0.991) on the genomic DNA level. This shows that PFGE is a very discriminative genotypic tool, and also that multiple clones of S. typhimurium isolates had existed in domestic animals and humans in Korea since 1993. However, we could ultimately not to trace the definitive sources or animal reservoirs of specific S. typhimurium isolates examined in this study. Depending on the SID values, the combined method (7 kinds of method) was found to be the most discriminative method, followed by (in order) SERE-PCR, REP-PCR, ERIC-PCR, PFGE & OPB-17 (RAPD), P-1254 (RAPD), and BOX-PCR at the $80\%$ clone cut-off value. This finding suggests that the REP-PCR method (which utilizes 4 primer types) may be an alternative tool to PFGE for the genotyping of S. typhimurium isolates, with comparable cost, time, and labor requirement. The establishment of a highly reliable and discriminatory method for epidemiologic analysis is considered necessary in order for researchers to trace the sources of specific pathogens and, consequently, to control and prevent the spread of epidemic S. typhimurium isolates to humans.

• Journal of Biomedical Engineering Research
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• v.37 no.5
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• pp.186-194
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• 2016

In this study, a co-axial flow induced microfluidic chip to fabricate pure collagen type I microfiber via the control of collagen type I and Na-alginate gelation process. The pure collagen type I microfiber was generated by selective degradation of Ca-alginate from 'Core-Shell' structured hydrogel microfiber. To make 'Core-Shell' structure, collagen type I solution was introduced into core channel and 1.5% Na-alginate solution was injected into side channel in microfluidic chip. To evaluatethe 'Core-Shell' structure, the red and green fluorescence substances were mixed into collagen type I and Na-alginate solution, respectively. The fluorescence substances were uniformly loaded into each fiber, and the different fluorescence images were dependent on their location. By immoblizing EpH4-Ras and C6 cells within collagen type I and Na-alginate solution, we sucessfully demonstrated the co-culture of EpH4-Ras and C6 cells with 'Core-Shell' like hydrogel microfiber for 5 days. Only to produce pure collagen type I hydrogel fiber, tri-sodium citrate solution was used to dissolve the shell-like Ca-alginate hydrogel fiber from 'Core-Shell' structured hydrogel microfiber, which is an excellent advantage when the fiber is employed in three-dimensional scaffold. This novel method could apply various application in tissue engineering and biomedical engineering.

• Geomechanics and Engineering
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• v.22 no.1
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• pp.1-9
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• 2020

This paper presents an investigation of the ground response of a gob-side gateroad suffering mining stress induced by a 21 m-thick coal seam extraction. A field observation, including entry convergence and stress changes monitoring, was first conducted in the tailgate 8209. The observation results of entry convergence showed that, during the adjacent panel 8210 retreating period, the deformation of the gob-side gateroad experienced a continuous increase stage, subsequently, an accelerating increase stage, and finally, a slow increase stage. However, strong ground response, including roof bending deflection, rib extrusion and floor heave, occurred during the current panel 8209 retreating period, and the maximum floor heave reached 1530 mm. The stress changes within coal mass of the two ribs demonstrated that the gateroad was always located in the stress concentrated area, which responsible for the strong response of the tailgate 8209. Subsequently, a hydraulic fracture technique was proposed to pre-fracture the two hard roofs above the tailgate 8209, thus decreasing the induced disturbance on the tailgate. The validity of the above roof treatment was verified via field application. The finding of this study could be a reference for understanding the stability control of the gob-side gateroad in extra thick coal seams mining.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.1445-1449
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• 2005

The ram assembly is important equipment in fueling machine of PHWR(Pressurized Heavy Water Reactor) plant where fuel replacement is possible while the plant is in service. Troubles in the ram assembly can cause lots of difficulties in power plant operation. The ram assembly is typically composed of the B-ram, the L-Ram and the C-Ram. The B-ram is focused in this paper because it plays the most important role in the ram assembly. Among the ram fault phenomena, ram stuck phenomena are the most frequent cases in the B-ram, which has a ball screw mechanism driven by a hydraulic motor. Ram stuck phenomena are due to ball wear and damage in ball nut that increase in proportion to the number of fuel replacement. It is required to predict ram stuck phenomena before they occur. In this paper, a method is proposed for predicting ram stuck phenomena using a discrete wavelet transform. The discrete wavelet transform provides information on both the time and frequency characteristics of the input signals. The proposed method uses the frequency bandwidths of coefficients of discrete wavelet decompositions and detail coefficients of discrete wavelet transform to predict ram stuck phenomena. The signal used in this paper is a torque-related signal such as a hydraulic service outlet pressure signal in a hydraulic driving system or a current signal in a DC motor driving system. Finally, the validity of the proposed method is shown via experiment using ball nut characteristic test equipment that simulates ram stuck phenomena due to increased ball friction in ball nut.

• Journal of the Korea Society of Computer and Information
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• v.25 no.7
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• pp.225-237
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• 2020

This study verified the effects that a programming-based TPACK education program, through design-based research on TPACK, had on pre-service teachers. The research subjects were pre-service teachers attending teacher training at a university in Korea, and 129 control groups and 126 experimental groups were recruited through lectures. The treatment used a TPACK educational program developed in a design-based study (Kim & Lee, 2019). A TPACK test tool developed by Park and Kang (2014) was used. The test results were analyzed via independent sample t-test and paired sample t-test. The results show that the programming-based TPACK education program (first cycle) was only effective in improving TPK and TPACK, but an effective educational program was developed to improve PCK, TCK, TK (second cycle), PK (third cycle), and CK (final cycle). This design-based research therefore confirmed the development of a programming-based TPACK education program that was effective for improving the TPACK of pre-service teachers.

• Molecules and Cells
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• v.40 no.6
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• pp.401-409
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• 2017

The primary cilium is a non-motile microtubule-based organelle that protrudes from the surface of most human cells and works as a cellular antenna to accept extracellular signals. Primary cilia assemble from the basal body during the resting stage ($G_0$ phase) and simultaneously disassemble with cell cycle re-entry. Defective control of assembly or disassembly causes diverse human diseases including ciliopathy and cancer. To identify the effective compounds for studying primary cilium disassembly, we have screened 297 natural compounds and identified 18 and 17 primary cilium assembly and disassembly inhibitors, respectively. Among them, the application of KY-0120, identified as Brefeldin A, disturbed Dvl2-Plk1-mediated cilium disassembly via repression of the interaction of $CK1{\varepsilon}-Dvl2$ and the expression of Plk1 mRNA. Therefore, our study may suggest useful compounds for studying the cellular mechanism of primary cilium disassembly to prevent ciliopathy and cancer.

• Journal of IKEEE
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• v.19 no.3
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• pp.357-365
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• 2015

Recently, embedded systems such as aircraft and automobilie, are developed as modular architecture instead of federated architecture because of SWaP(Size, Weight and Power) issues. In addition, partition operating system that support multiple logical node based on partition concept were recently appeared. Distributed recovery block is fault tolerance design scheme that applicable to mission critical real-time system to support real-time take over via real-time synchronization between participated nodes. Because of real-time synchronization, single-core based computer is not suitable for partition based distributed recovery block design scheme. Multi-core and AMP(Asymmetric Multi-Processing) based partition architecture is required to apply distributed recovery block design scheme. In this paper, we proposed design scheme of distributed recovery block on the multi-core based supervised-AMP architecture partition operating system. This paper implements flight control simulator for avionics to check feasibility of our design scheme.

• Journal of KIISE
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• v.44 no.11
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• pp.1194-1208
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• 2017

A traditional average smoothing method is designed for smoothing out noise, which, however, unintentionally results in smooth corner points on the curvature accompanied with a shrinkage of curves. In this paper, we propose a novel curve smoothing method via polygonal approximation of the input curve. The proposed method determines the smoothing weight for each point of the input curve based on the angle and approximation error between the approximated polygon and the input curve. The weight constrains a displacement of the point after smoothing not to significantly exceed the average noise error of the region. In the experiment, we observed that the resulting smoothed curve is close to the original curve since the point moves toward the average position of the noise after smoothing. As an application to digital cartography, for the same amount of smoothing, the proposed method yields a less area reduction even on small curve segments than the existing smoothing methods.

• The Korean Journal of Applied Statistics
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• v.30 no.5
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• pp.615-632
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• 2017

Unlike randomized trial, statistical strategies for inferring the unbiased causal relationship are required in the observational studies. The matching with the propensity score is one of the most popular methods to control the confounders in order to evaluate the effect of the treatment on the outcome variable. Recently, new methods for the causal inference in latent class analysis (LCA) have been proposed to estimate the average causal effect (ACE) of the treatment on the latent discrete variable. They have focused on the application study for the real dataset to estimate the ACE in LCA. In practice, however, the true values of the ACE are not known, and it is difficult to evaluate the performance of the estimated the ACE. In this study, we propose a method to generate a synthetic data using the propensity score in the framework of LCA, where treatment and outcome variables are latent. We then propose a new method for estimating the ACE in LCA and evaluate its performance via simulation studies. Furthermore we present an empirical analysis based on data form the 'National Longitudinal Study of Adolescents Health,' where puberty as a latent treatment and substance use as a latent outcome variable.

• Molecules and Cells
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• v.19 no.1
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• pp.67-73
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• 2005

Isoflavones are synthesized by isoflavone synthases via the phenylpropanoid pathway in legumes. We have cloned two isoflavone synthase genes, IFS1 and IFS2, from a total of 18 soybean cultivars. The amino acid residues of the proteins that differed between cultivars were dispersed over the entire coding region. However, amino acid sequence variation did not occur in conserved domains such as the ERR triad region, except that one conserved amino acid was changed in the IFS2 protein of the GS12 cultivar ($R_{374}G$) and the IFS1 proteins of the 99M06 and Soja99s65 cultivars ($A_{109}T$, $F_{105}I$). In three cultivars (99M06, 99M116, and Simheukpi), most of amino acid changes were such that the difference between the amino acid sequences of IFS1 and IFS2 was reduced. The expression profiles of three enzymes that convert naringenin to the isoflavone, genistein, chalcone isomerase (CHI), isoflavone synthase (IFS) and flavanone 3-hydroxylase (F3H) were examined. In general, IFS mRNA was more abundant in etiolated seedlings than mature plants whereas the levels of CHI and F3H mRNAs were similar in the two stages. During seed development, IFS was expressed a little later than CHI and F3H but expression of these three genes was barely detectable, if at all, during later seed hardening. In addition, we found that the levels of CHI, F3H, and IFS mRNAs were under circadian control. We also showed that IFS was induced by wounding and by application of methyl jasmonate to etiolated soybean seedlings.