• Archives of Pharmacal Research
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• 제20권5호
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• pp.404-409
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• 1997

Trout CYP1A-CAT expression construct was generated by cloning -3.5 Kb $5^I$ flanking DNA of trout liver CYP1A gene in front of CAT gene at pCAT-basic vector. Hepa 1 cells, which are known to contain a functional arylhydrbcarbon $receptor^I$ were transfected with trout CYP1A-CAT using lipofectin. 3-Methylcholanthrene (1 nM) was added into hepa 1 cells in culture in order to examine if $5^I$ flanking DNA of trout CYP1A gene could interact with mouse transactivating factors to bring about transcription of the chloramphenicol acetyltransferase(CAT) reporter gene. The level of CAT protein was measured by CAT ELISA and the level of CAT mRNA was determined by RTPCR. The treatment of 1 nM 3-methylcholanthrene resulted in two fold increases in CAT protein as well as CAT mRNA compared to untreated control hepa 1 cells. These data indicate that arylhydrocarbon receptors of mouse hepa 1 cells are functional to activate exogenously transfected trout CYP1A-CAT construct in terms of both transcription and translation of CAT. We also examined the effect of 3-methylcholanthrene on endogenous cyplal activity in hepa 1 cell. 3-Methylcholanthrene (1 nM) treatment to hepa 1 cells trahsfected with trout CYP1A-CAT construct stimulated the level of cyp1a1 mRNA by two folds and the activity of ethoxyresorufin-O-deethylase by two fold compared to that of control cells. In this study we reported that trout CYP1A-CAT reporter gene expression construct could be expressed by 3-methylcholanthrene treatment in mouse hepa 1 cells. Thus trout CYP1A-CAT could serve as a good model to study the mechanism of regulation of CYP1A1 gene expression.

• 산학경영연구
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• 제20권1호
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• pp.73-103
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• 2007

본 연구의 목적은 인적자원훈련기관에서의 변혁적리더십 특성이 직무만족과 조직몰입에 미치는 영향에 관한 연구를 한 것이다. 본 연구의 발견사항은 변혁적 리더십과 직무만족간의 통계적으로 유의한 관계가 있는 것으로 나타났다. 특히, 변혁적리더십의 하위개념인 카리스마요인과 직무만족 개념과도 유의적인 관계가 있는 것으로 나타났다. 둘째, 변혁적 리더십과 조직몰입간에도 유의적인 정(+)의 관계가 있는 것으로 나타났다. 특히 변혁적인 리더십의 하위개념인 지적자극요인과 영감적동기요인은 조직몰입과 유의적인 정(+)의 관계가 있는 것으로 나타났다. 이러한 연구결과는 변혁적인 리더십이 직무만족과 조직몰입에 영향을 미치는 것으로 나타났다.

• 대한가정학회지
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• 제41권5호
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• pp.179-191
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• 2003

The study is to suggest easier making method of skirts and slacks as to compare production process between manufacturers and references of clothing construct in universities. Skirts and slacks have simple production process and they are basic subjects in clothing construct class. But most references have just contents which are to make basic styles of skirts and slacks made basic materials. Continuous development of new material and variety of style request more efficient and easer making method in the reference of clothing construct. So the making methods of references and manufacturers are compared and analyzed in this study In references, there are many hand work and sub-work because they are for a person who make own clothes. In contrast, manufacturers have to product many variety clothes of good quality for many people and think the production cost of clothes. They use more simple and efficient method that include their know-how to meet changing fashion situation. In making methods of skirts and slacks, differences between references and manufacturer are in sewing of waist band and zippers and location of interlining. Manufacturers use variedly sewing method of waist band according to material and their experience. Especially handling for wrong side inseam of waist band is distinct between references and manufacturers. References have basic and detail work and manufacturers know processing order having even grade work to keep continuous production. The making method of clothes have to change and improve according to the situation. The more useful making method get to include their merits and references need to add more practical method and knowledge.

• 대한기계학회논문집B
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• 제35권1호
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• pp.9-15
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• 2011

본 연구에서는 3 차원 전산 열유동해석을 통하여 형상법칙에 근거하여 개발된 냉각판의 열수력학적인 특성을 분석하였다. 서펜타인 형상을 포함하여 최적화, 최적화되지 않은 1, 2 차 형상 총 5 개 형상을 대상으로 동일한 구속조건을 부여함으로써 유동저항, 열저항 및 분지유로에서의 유동균일도를 상호 비교함으로써 냉각판의 성능평가를 수행하였다. 그 결과, 최적화된 1, 2 차 형상 구조가 최적화되지 않은 경우와 비교하여 훨씬 적은 압력손실을 나타내었으며, 압력손실을 기준으로 최적화된 2 차 형상 구조가 가장 우수한 유동 구조를 나타내었다. 또한, 최적화된 1, 2 차 형상구조의 열저항 및 유동 분배성능도 기존에 사용되는 유로형상과 비교하여 모두 우수한 성능을 보였다.

• Journal of Korean Neurosurgical Society
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• 제46권5호
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• pp.431-436
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• 2009

Objective : This study examines whether functional motion is present at one or more years after Bioflex System placement. BioFlex System is a flexible rod system which has been used to preserve motion at the area of implantation. There has not been a scientific study showing how much motion is preserved after implantation. Methods : A total of 12 consecutive patients underwent posterior dynamic stabilization using the BioFlex System. Six patients were treated using a L3-4-5 construct and other six patients using a L4-5-S1 construct. Follow-up ranged from 12 to 33 months and standing neutral lateral, extension, flexion and posteroanterior (PA) radiographs were obtained at 3, 6, 9, and 12 months and at more than 12 months postoperatively. Range of motion (ROM), whole lumbar lordosis, and ROMs of motion segments from L2 to S1 were determined. Results : Patients with a L3-4-5 construct demonstrated a decrease in mean ROM for whole lumbar decreased from 40.08 to 30.77. Mean ROM for L3-4 (6.12 to 2.20) and L4-5 (6.55 to 1.67) also decreased after one year. Patients with a L4-5-S1 construct demonstrated L4-5 (8.75 to 2.70) and L5-S1 (9.97 to 3.25) decrease of mean ROM at one year postoperatively. Lumbar lordosis was preservep at both L3-4-5 and L4-5-S1 constructs. Clinical results showed significant improvements in both study groups. Conclusion : The present study provides preliminary information regarding the BioFlex motion preservation system. We conclude that the BioFlex System preserves functional motion to some degree at instrumented levels. However, although total lumbar lordosis was preserved, ROMs at implantation segments were lower than preoperative values.

• Journal of Nutrition and Health
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• 제45권4호
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• pp.390-399
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• 2012

The objective of this study was to develop and evaluate the construct validity of a Nutrition Quotient (NQ) for children. In a previous report (Kang, et al., 2012), the food behavior checklist for children's NQ, consisting of 19 items, was grouped into a 5-factor structure according to the exploratory factor analysis: balance, diversity, moderation, regularity, and practice. In this study, the construct validity of the NQ was assessed using a confirmatory factor analysis. Elementary school students (n = 1,393) from six large cities completed the NQ test. Indicator tests suggested an adequate model fit (goodness of fit index = 0.9613; adjusted GFI = 0.95; standardized root mean square residual = 0.0464; chi-square test statistics of < 0.001 p-value, 82.1), and item loadings were significant for all subscales (p < 0.05). The standardized path coefficients were used as the weights of the items. The NQ and the 5 factor scores of the student were calculated by the obtained weights of the questionnaire items. Logistic regression was applied to find the significant factors in order to affect a specific nutrient status. The receiver operation characteristic curve analyses were performed in order to find diagnostic cut-off points of the five factors. The food behavior checklist for children's NQ would be a handy and suitable instrument for evaluating dietary behaviors of Korean children.

• 한국전문물리치료학회지
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• 제20권3호
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• pp.89-99
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• 2013

The purposes of this study were: 1) to show the item difficulty hierarchy of walking/moving construct of the International Classification of Functioning, Disability and Health-Activity Measure (ICF-AM), 2) to evaluate the item-level psychometrics for model fit, 3) to describe the relevant physical activity defined by level of activity intensity expressed as Metabolic Equivalent of Tasks (MET), and 4) to explore what extent the empirical activity hierarchy of the ICF-AM is linked to the conceptual model based on the level of energy expenditure described as MET. One hundred and eight participants with lower extremity impairments were examined for the present study. A newly created activity measure, the ICF-AM using an item response theory (IRT) model and computer adaptive testing (CAT) method, has a construct on walking/moving construct. Based on the ICF category of walking and moving, the instrument comprised items corresponding to: walking short distances, walking long distances, walking on different surfaces, walking around objects, climbing, and running. The item difficulty hierarchy was created using Winstep software for 20 items. The Rasch analyses (1-parameter IRT model) were performed on participants with lower extremity injuries who completed the paper and pencil version of walking/moving construct of the ICF-AM. The classification of physical activity can also be performed by the use of METs that is often preferred to determine the level of physical activity. The empirical item hierarchy of walking, climbing, running activities of the ICF-AM instrument was similar to the conceptual activity hierarchy based on the METs. The empirically derived item difficulty hierarchy of the ICF-AM may be useful in developing MET-based activity measure questionnaires. In addition to convenience of applying items to questionnaires, implications of the finding could lead to the use of CAT method without sacrificing the objectivity of physiologic measures.

• The Plant Pathology Journal
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• 제25권2호
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• pp.136-141
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• 2009

Marking biocontrol bacteria is an essential step to monitor bacterial behavior in natural environments before application in agricultural ecosystem. In this study, we presented the simple green fluorescent protein (GFP) reporter system driven by the promoter active in Bacillus species for tagging of the biocontrol bacteria. A constitutive promoter P43 from Bacillus subtilis was fused to an enhanced promoterless gfp gene by overlap extension PCR. The GFP expression was demonstrated by the high fluorescence intensity detected in B. subtilis and Escherichia coli transformed with the P43-gfp fusion construct, respectively. The GFP reporter system was further investigated in two bacterial biocontrol strains B. licheniformis and Pseudomonas fluorescens. When the reconstructed plasmid pWH34G was introduced into B. licheniformis, GFP level measured with the fluorescence intensity in B. licheniformis was almost equivalent to that in B. subtilis. However, GFP expression level was extremely low in other biocontrol bacteria P. fluorescens by transposon based stable insertion of the P43-gfp construct into the bacterial chromosome. This study provides information regarding to the efficient biomarker P43-gfp fusion construct for bio-control Bacillus species.

• 정보처리학회논문지D
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• 제16D권3호
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• pp.417-422
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• 2009

학술적 중요성 이외에 실무적으로도 정보시스템 효과성 측정을 위한 측정지표의 타당성 검증은 중요하다고 할 수 있다. 최근 정보시스템 또는 정보기술에 대한 투자가 급증함에 따라 민간기업에서도 IT 투자의 비즈니스 가치에 대한 관심이 높아지고 있다. 한편 민간부문뿐만 아니라 공공부문에서도 한정된 국가예산을 가지고 투입되는 정보화 부문에 대한 투자효과 평가의 필요성이 부각되고 있다. 최근 여러 정부부처별로 진행되고 있는 공공 정보화사업의 체계적이고 비교가 가능한 성과평가를 위해서 공공정보시스템 효과성의 개념을 정의하고, 이를 측정하기 위한 측정지표의 개발 및 타당성 검증이 요구되고 있다. 이에 따라 행정정보DB구축사업을 대상으로 공공정보시스템 효과성 측정지표의 타당성을 검증해 보았다.

• Steel and Composite Structures
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• 제43권4호
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• pp.447-456
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• 2022

Recent experimental studies showed that deep steel I-shaped profiles classified as high ductility class sections in seismic design international codes exhibit low deformation capacity when subjected to cyclic loading. This paper presents an innovative retrofit solution to increase the rotation capacity of beams using bonded carbon fiber reinforced polymers (CFRP) patches validated with advanced finite element analysis. This investigation focuses on the flexural cyclic behaviour of I-shaped hot rolled steel deep section used as beams in moment-resisting frames (MRF) retrofitted with CFRP patches on the web. The main goal of this CFRP reinforcement is to increase the rotation capacity of the member without increasing the overstrength in order to avoid compromising the strong column-weak beam condition in MRF. A finite element model that simulates the cyclic plasticity behavior of the steel and the damage in the adhesive layer is developed. The damage is modelled using the cohesive zone modelling (CZM) technique that is able to capture the crack initiation and propagation. Details on the modelling techniques including the mesh sensitivity near the fracture zone are presented. The effectiveness of the retrofit solution depends strongly on the selection of the appropriate adhesive. Different adhesive types are investigated where the CZM parameters are calibrated from high fidelity fracture mechanics tests that are thoroughly validated in the literature. This includes a rigid adhesive commonly found in the construction industry and two tough adhesives used in the automotive industry. The results revealed that the CFRP patch can increase the rotation capacity of a steel member considerably when using tough adhesives.