• Genomics & Informatics
• /
• 제19권4호
• /
• pp.47.1-47.12
• /
• 2021

Kaposi's sarcoma-associated herpesvirus (KSHV) is one of the few human oncogenic viruses, which causes a variety of malignancies, including Kaposi's sarcoma, multicentric Castleman disease, and primary effusion lymphoma, particularly in human immunodeficiency virus patients. The currently available treatment options cannot always prevent the invasion and dissemination of this virus. In recent times, siRNA-based therapeutics are gaining prominence over conventional medications as siRNA can be designed to target almost any gene of interest. The ORF57 is a crucial regulatory protein for lytic gene expression of KSHV. Disruption of this gene translation will inevitably inhibit the replication of the virus in the host cell. Therefore, the ORF57 of KSHV could be a potential target for designing siRNA-based therapeutics. Considering both sequence preferences and target site accessibility, several online tools (i-SCORE Designer, Sfold web server) had been utilized to predict the siRNA guide strand against the ORF57. Subsequently, off-target filtration (BLAST), conservancy test (fuzznuc), and thermodynamics analysis (RNAcofold, RNAalifold, and RNA Structure web server) were also performed to select the most suitable siRNA sequences. Finally, two siRNAs were identified that passed all of the filtration phases and fulfilled the thermodynamic criteria. We hope that the siRNAs predicted in this study would be helpful for the development of new effective therapeutics against KSHV.

• Journal of Animal Science and Technology
• /
• 제64권1호
• /
• pp.110-122
• /
• 2022

The reduction of milk yield caused by heat stress in summer is the main condition restricting the economic benefits of dairy farms. To examine the impact of hyperthermia on bovine mammary epithelial (MAC-T) cells, we incubated the MAC-T cells at thermal-neutral (37℃, CON group) and hyperthermic (42℃, HS group) temperatures for 6 h. Subsequently, the cell viability and apoptotic rate of MAC-T cells, apoptosis-related genes expression, casein and amino acid transporter genes, and the expression of the apoptosis-related proteins were examined. Compared with the CON group, hyperthermia significantly decreased the cell viability (p < 0.05) and elevated the apoptotic rate (p < 0.05) of MAC-T cells. Moreover, the expression of heat shock protein (HSP)70, HSP90B1, Bcl-2-associated X protein (BAX), Caspase-9, and Caspase-3 genes was upregulated (p < 0.05). The expression of HSP70 and BAX (pro-apoptotic) proteins was upregulated (p < 0.05) while that of B-cell lymphoma (BCL)2 (antiapoptotic) protein was downregulated (p < 0.05) by hyperthermia. Decreased mRNA expression of mechanistic target of rapamycin (mTOR) signaling pathway-related genes, amino acid transporter genes (SLC7A5, SLC38A3, SLC38A2, and SLC38A9), and casein genes (CSNS1, CSN2, and CSN3) was found in the heat stress (HS) group (p < 0.05) in contrast with the CON group. These findings illustrated that hyperthermia promoted cell apoptosis and reduced the transport of amino acids into cells, which inhibited the milk proteins synthesis in MAC-T cells.

• Advances in concrete construction
• /
• 제13권 2호
• /
• pp.163-181
• /
• 2022

To study the mechanical properties of steel reinforced recycled concrete (SRRC) filled circular steel tube columns under eccentric compression loads, this study presents a finite element model which can simulate the eccentrically compressed columns using ABAQUS software. The analytical model was established by selecting the reasonable nonlinear analysis theory and the constitutive relationship of materials in the columns. The influences of design parameters on the eccentric compressive performance of columns were also considered in detail, such as the diameter-thickness ratio of circular steel tube, replacement percentage of recycled coarse aggregate (RCA), slenderness ratio, eccentricity, recycled aggregate concrete (RAC) strength and steel strength and so on. The deformation diagram, stress nephogram and load-displacement curves of the eccentrically compressed columns were obtained and compared with the test results of specimens. The results show that although there is a certain error between the calculation results and the test results, the error is small, which shows the rationality on the numerical model of eccentrically compressed columns. The failure of the columns is mainly due to the symmetrical bending of the columns towards the middle compression zone, which is a typical compression bending failure. The eccentric bearing capacity and deformation capacity of columns increase with the increase of the strength of steel tube and profile steel respectively. Compared with profile steel, the strength of steel tube has a greater influence on the eccentric compressive performance of columns. Improving the strength of RAC is beneficial to the eccentric bearing capacity of columns. In addition, the eccentric bearing capacity and deformation capacity of columns decrease with the increase of replacement percentage of RCA. The section form of profile steel has little influence on the eccentric compression performance of columns. On this basis, the calculation formulas on the nominal eccentric bearing capacity of columns were also put forward and the results calculated by the proposed formulas are in good agreement with the test values.

• Smart Structures and Systems
• /
• 제32권1호
• /
• pp.9-21
• /
• 2023

The parameters of civil engineering structures have time-variant characteristics during their service. When extremely large external excitations, such as earthquake excitation to buildings or overweight vehicles to bridges, apply to structures, sudden or gradual damage may be caused. It is crucially necessary to detect the occurrence time and severity of the damage. The unscented Kalman filter (UKF), as one efficient estimator, is usually used to conduct the recursive identification of parameters. However, the conventional UKF algorithm has a weak tracking ability for time-variant structural parameters. To improve the identification ability of time-variant parameters, an adaptive UKF with forgetting factor (AUKF-FF) algorithm, in which the state covariance, innovation covariance and cross covariance are updated simultaneously with the help of the forgetting factor, is proposed. To verify the effectiveness of the method, this paper conducted two case studies as follows: the identification of time-variant parameters of a simply supported bridge when the vehicle passing, and the model updating of a six-story concrete frame structure with field test during the Yangbi earthquake excitation in Yunnan Province, China. The comparison results of the numerical studies show that the proposed method is superior to the conventional UKF algorithm for the time-variant parameter identification in convergence speed, accuracy and adaptability to the sampling frequency. The field test studies demonstrate that the proposed method can provide suggestions for solving practical problems.

• Steel and Composite Structures
• /
• 제49권4호
• /
• pp.441-456
• /
• 2023

Aiming at the development trend of light weight and high strength of engineering structures, this paper experimentally investigated the seismic performance of circular-in-square high-strength concrete-filled double skin steel tubular (HCFDST) stub columns with out-of-code width-to-thickness (B/t) ratios. Typical failure mode of HCFDST stub columns appeared with the infill material crushing, steel fracture and local buckling of outer tubes as well as the inner buckling of inner tubes. Subsequently, the detailed analysis on hysteretic curves, skeleton curves and ductility, energy dissipation, stiffness degradation and lateral force reduction was conducted to reflect the influences of hollow ratios, axial compression ratios and infill types, e.g., increasing hollow ratio from 0.54 to 0.68 and 0.82 made a slight effect on bearing capacity compared to the ductility coefficients; the higher axial compression ratio (e.g., 0.3 versus 0.1) significantly reduced the average bearing capacity and ductility; the HCFDST column SCFST-6 filled with concrete obviously displayed the larger initial secant stiffness with a percentage 34.20% than the column SCFST-2 using engineered cementitious composite (ECC); increasing hollow ratios, axial compression ratios could accelerate the drop speed of stiffness degradation. The out-of-code HCFDST stub columns with reasonable design could behave favorable hysteretic performance. A theoretical model considering the tensile strength effect of ECC was thereafter established and verified to predict the moment-resisting capacity of HCFDST columns using ECC. The reported research on circular-in-square HCFDST stub columns can provide significant references to the structural application and design.

• Earthquakes and Structures
• /
• 제26권1호
• /
• pp.77-86
• /
• 2024

To investigate the seismic performance of steel pipe-aeolian sand recycled concrete columns, this study designed and produced five specimens. Low-cycle repeated load tests were conducted while maintaining a constant axial compression ratio. The experiment aimed to examine the impact of different aeolian sand replacement rates on the seismic performance of these columns. The test results revealed that the mechanical failure modes of the steel pipe-recycled concrete column and the steel pipe-aeolian sand recycled concrete column were similar. Plastic hinges formed and developed at the column foot, and severe local buckling occurred at the bottom of the steel pipe. Interestingly, the bulging height of the damaged steel pipe was reduced for the specimen mixed with an appropriate amount of wind-deposited sand under the same lateral displacement. The hysteresis curves of all five specimens tested were relatively full, with no significant pinching phenomenon observed. Moreover, compared to steel tube-recycled concrete columns, the steel tube-aeolian sand recycled concrete columns exhibited improved seismic energy dissipation capacity and ductility. However, it was noted that as the aeolian sand replacement rate increased, the bearing capacity of the specimen increased first and then decreased. The seismic performance of the specimen was relatively optimal when the aeolian sand replacement rate was 30%. Upon analysis and comparison, the damage analysis model based on stiffness and energy consumption showed good agreement with the test results and proved suitable for evaluating the damage degree of steel pipe-wind-sand recycled concrete structures.

• Advances in nano research
• /
• 제16권3호
• /
• pp.231-249
• /
• 2024

When the amplitude of the vibrations is equivalent to that clearance, the vibrations for small amplitudes will really be significantly nonlinear. Nonlinearities will not be significant for amplitudes that are rather modest. Finally, nonlinearities will become crucial once again for big amplitudes. Therefore, the concrete panel system may experience a big amplitude in this work as a result of the high temperature. Based on the 3D modeling of the shell theory, the current work shows the influences of the von Kármán strain-displacement kinematic nonlinearity on the constitutive laws of the structure. The system's governing Equations in the nonlinear form are solved using Kronecker and Hadamard products, the discretization of Equations on the space domain, and Duffing-type Equations. Thermo-elasticity Equations. are used to represent the system's temperature. The harmonic solution technique for the displacement domain and the multiple-scale approach for the time domain are both covered in the section on solution procedures for solving nonlinear Equations. An effective data-driven solution is often utilized to predict how different systems would behave. The number of hidden layers and the learning rate are two hyperparameters for the network that are often chosen manually when required. Additionally, the data-driven method is offered for addressing the nonlinear vibration issue in order to reduce the computing cost of the current study. The conclusions of the present study may be validated by contrasting them with those of data-driven solutions and other published articles. The findings show that certain physical and geometrical characteristics have a significant effect on the existing concrete panel structure's susceptibility to temperature change and GPL weight fraction. For building construction industries, several useful recommendations for improving the thermo-mechanics' behavior of structural concrete panels are presented.

• 한국콘텐츠학회논문지
• /
• 제12권10호
• /
• pp.195-204
• /
• 2012

부산 도시공원의 시설과 서비스 콘텐츠에 대한 경험적 검증을 토대로 공원복지성의 수준을 파악하고 각 개별 도시공원의 전략적 발전방안 제시가 본연구의 목적이다. 본 연구는 전문가의 평판법에 의해 추천된 부산시 7개 도시공원 이용자 1500명을 대상으로 설문조사로 진행되었다. 도시공원 이용형태와 시설 및 콘텐츠의 공원복지수준의 IPA 분석결과는 다음과 같다. 첫째, 부산시 도시공원 이용자의 공원복지성을 구성하고 있는 이용도와 만족도는 모든 항목에서 유의미한 차이가 있는 것으로 나타났다. 또한 도시공원 선행연구들과 유사하게 공원복지 시설 중 편의시설이 집중관리 항목으로 나타났다. 둘째, 부산이라는 동일한 지역에 동일한 도시공원이라는 유형이라 할지라도 주 이용자와 소재지의 지역적 특성에 따라서 집중영역의 차이가 있는 것으로 나타났다. 본 연구에서 상정된 두 가지 연구문제에서 제기된 유의미한 차이가 모두 인정되었다. 결론적으로 부산의 도시공원 운영에 있어서 이용객들의 공원복지에 대한 기대를 충족시키지 못한다고 해석 할 수 있다. 본 연구는 동일한 유형의 도시공원이라 할지라도 집중관리영역의 차이를 공원복지적 관점에서 경험적인 검증을 했다는데 연구의 의의를 둘 수 있다. 시민참여와 지역네트워크의 새로운 공원운영 매커니즘인 컨저번시가 공원복지 성과의 가속화를 위한 앞으로의 과제가 될 수 있을 것이다.

• 한국조경학회지
• /
• 제39권3호
• /
• pp.74-82
• /
• 2011

본 연구는 공원관리 참여조직간의 사회적 연결망을 분석하고, 참여조직의 활동을 활성화하는 방향을 모색하기 위한 것이다. 이를 위해서 공원관리 참여조직 간의 신뢰, 참여, 정보교류의 실태를 사회 연결망 분석을 통해 살펴보았다. 구체적으로 서울숲을 대상으로 하였으며, 행정기관, 시민단체, 주민단체, 기업으로 구성된 15개 참여조직과 핵심인물 30명을 조사대상으로 선정하였다. 설문조사는 조직간 신뢰, 참여, 정보교류 및 연구 통계적 속성에 관한 내용으로 구성되었으며, 분석은 사회 연결망 분석 프로그램인 UCINET을 활용하였다. 분석결과, 1) 신뢰, 참여, 정보교류의 연결구조에서 다른 조직에 비해 많은 관계를 유지하고 있는 중추조직(HUB)는 서울숲 사랑모임과 서울숲 관리사무소로 나타났다. 2) 함께 일하고 싶은 조직과 향후 활발한 참여를 바라는 조직에 대한 연결망 분석에서는 환경 예술분야의 시민단체에 대한 요구가 높게 나타났다. 3) 부녀회, 통장단 등 지역 주민단체 연결망을 살펴보면 중심성이 매우 낮으며 서로간의 연결관계도 거의 없는 것으로 나타났다. 본 연구결과와 함께 다양한 도시공원과 지역을 대상으로 공원관리 참여조직간의 사회 연결망 실태를 파악함으로써 주민참여와 협력 이 활발한 공원관리 방안을 마련하는데 활용할 수 있다.

• 한국환경생태학회지
• /
• 제7권1호
• /
• pp.22-28
• /
• 1993

근래 들어 급속한 산업화와 그에 따른 무분별한 개발 등으로 생태계의 파괴가 빠른 속도로 진행되고 이와 같은 생태계의 파괴는 많은 생물종의 서식지 박탈로 이어져 수많은 종이 멸종되거나 멸종위기에 처해 있다. 민주지산지역 역시 예외가 아니어서 1992년 영동군은 상기 자연생태지역에 종합관광휴양지 건설(동신레저 주식회사)을 추진하고 있다. 이를 위해 6,000,000$m^2$에 이르는 산림보존지역을 스키장과 골프장을 위한 개발촉진지역 및 관광휴양지역으로 용도변경한 바 있다. 상기의 종합관광휴양지 개발로 인한 자연생태계 파괴가 조류 분포에 미치는 영향을 조사하기 위하여 개발 이전의 민주지산의 조류상을 선행 조사하였다. 민주지산 일대에서 관찰된 조류는 총 9목 23과 41종 229개체로 민주지산에서 9목 21과 39종 158개체, 석기봉에서 5목 16과 27종 71개체가 각각 조사되었다. 동고비가 우점종이었으며 다음으로 진박새, 쇠솔새, 박새, 쇠박새, 횐배지빠귀의 순으로 우세하였다. 천연기념물 조류로는 새매, 붉은배새매 및 소쩍새가 관찰되었고 희귀종 청호반새도 출현하였다. 종다양성 지수가 3.381로 이 값은 지리산(3.400) 이외의 타 지역보다 높았다. 민주지산, 석기봉, 삼도봉 일대는 인적이 드물고 환경오염이 적어 타 지역에 비하여 조류의 서식이 많은 지역이다. 또한 태백산맥과 소백산맥이 연결되는 생물지지 학적으로 매우 중요한 곳으로 조류를 비롯한 많은 동물의 이동로이다. 상기 지역과 연결되어 있는 국립공원 덕유산에 이미 건설되었거나 건설 예정인 각종 스포츠 레저시설로 생태계 훼손이 심각하게 우려되는 이 때 민주지산 일대의 대규모 개발은 자제되어야 할 것이다.