• Title/Summary/Keyword: Conducted Susceptibility

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Protein Patterns of Rice(Oryza sativa L.) Cultivars as Affected by Herbicide Thiobencarb (제초제 Thiobencarb 처리에 의한 수도품종간 단백질 유형의 변화)

  • Kim, H.Y.;Kim, K.U.;Shin, D.H.;Kim, K.W.
    • Korean Journal of Weed Science
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    • v.16 no.4
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    • pp.354-361
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    • 1996
  • This study was conducted to evaluate the susceptibility of rice(Oryza sativa L.) cultivars to herbicide thiobencarb through the determination of protein patterns using SDS-PAGE. In both greenhouse and laboratory screening tests, IR 10198-66-2 and IR 9660-50-3-1 showed relatively tolerant response to thiobencarb, while IR 22, IR 31802-48-2-2-2 and IR 20656-R-R-R-6-1 were susceptible to it. The total protein content of susceptible cultivars markedly decreased as the thiobencarb concentrations increased, but tolerant cultivars such as IR 10198-66-2 and IR 9660-50-3-1 showed very slight changes suggesting that protein synthesis of susceptible cultivars may be inhibited by thiobencarb. The protein profiles of the tolerant cultivars were not much affected by thiobencarb treatment. However, the protein spots with molecular weights of 14.4 kD and 55 kD in the susceptible cultivar of IR 22 disappeared with the treatment of 3 ppm thiobencarb, indicating that differential susceptibilities of rice cultivars against thiobencarb can be attributed to their difference in protein metabolism affected by thiobencarb.

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A Case-Control Study of Primary Liver Cancer and Liver Disease History (간 질환력과 원발성 간암에 관한 환자-대조군 연구)

  • Kim, Dong-Hyun;Park, Byung-Joo;Yoo, Keun-Young;Ahn, Yoon-Ok;Lee, Hyo-Suk;Kim, Chung-Yong;Lee, Sang-Il;Lee, Moo-Song;Ahn, Hyung-Sik;Kim, Heon;Park, Tae-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.27 no.2 s.46
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    • pp.217-225
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    • 1994
  • The relationship between past liver disease history and the risk of primary liver cancer was analyzed in a hospital-based case-control study conducted in Seoul on 165 patients with histologically or serologically confirmed hepatocellular carcinoma and individually age- and sex-matched 165 controls in hospital for ophthalmologic, otologic, or nasopharyngeal problems. Significant association were observed for liver deseases occurring 5 or more years before liver cancer diagnosis [OR,4.9;95% confidence interval (CI), $1.6{\sim}14.0$) and family history of liver disease(OR, 9.0;95% CI, $2.1{\sim}38.8$). These associations were not appreciably modified by allowance for major identified potential confounding factors. From these results, it is possible to speculate that liver cell injuries caused by various factors might be a common pathway to developing primary liver carcinoma. Considering the significant effect of family history of liver diseases on PLCA risk after adjusting past liver disease history, there might be genetic susceptibility in the carcinogenic mechanism of liver cancer. Further investigations are needed to clarify the effect of family history of liver disease on PLCA risk.

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Prevalence of the antimicrobial resistance and resistance associated gene in Salmonella spp. isolated from pigs and cattle in slaughterhouse (도축장의 소와 돼지 분변에서 분리한 살모넬라속의 약제내성 및 약제내성 유전자의 보유율)

  • Hah, Do-Yun;Ji, Dae-Hae;Jo, Sang-Rae;Park, Ae-Ra;Jung, Eun-Hee;Park, Dong-Yeop;Lee, Kuk-Cheon;Yang, Jung-Wung;Kim, Jong-Shu;Kim, Hye-Jung;Jung, Jong-Hwa;Song, Ick-Hyun;Kim, Ae-Ran;Lee, Ji-Youn;Kim, Young-Hwan
    • Korean Journal of Veterinary Service
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    • v.34 no.1
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    • pp.45-54
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    • 2011
  • This study was conducted to investigate the distribution of Salmonella spp. from pigs and cattle in slaughterhouse, the antimicrobial resistance pattern and the prevalence of resistance genes of isolates. A total of 640 fecal samples from pigs and cattle in slaughterhouse were collected for isolation of Salmonella spp.. Isolation rate was revealed as 15% in pigs and 1.6% in cattle. As result of serotyping, group B (56.6%) were identified as most common in pigs and cattle isolates, in order of group C (24.5%) and group E (15.1%). S. Typhimurium (50.9%) was most common serotype. The major serotypes were in order of S. Rissen and S. London (11.3%) and S. Riggil (7.6%). In antimicrobial test, all isolates were demonstrates susceptibility to nitrofurantoin. But isolates were revealed resistance other antibiotics in order of tetracycline (64.6%), streptomycin (68.3%), ampicillin and amoxicillin (56.3%) and spectinomycin (47.9%). With polymerase chain reaction, antimicrobial resistance gene strA (75.0%) and aadA1 (3.1%) were detected in streptomycin resistance isolates and tetA (94.3%) and tetB (11.3%) gene were detected in tetracycline resistant isolates, but tetG was not detected. Class 1 integron gene was detected in all Salmonella isolates.

In vivo characterization and transmission of Korean foot-and-mouth disease virus(FMDV) (국내 발생 구제역 바이러스(foot-and-mouth disease virus)의 특성과 전파력에 관한 연구)

  • Sur, Jung-hyang;Shin, Jin-ho;Loubroth, Juan;Yeh, Max;Ku, Bok-kyung;Choi, Kang-seuk;Kweon, Byung-joon;Sohn, Hyun-joo;Ko, Young-joon;Choi, Cheong-up;Kwon, Chang-hee;Kim, Jong-yeom;An, Soo-hwan;Kim, Ki-seuk;Moon, Oun-kyung;Kim, Jae-hoon;Choi, Sang-ho;Lee, Hong-gil;Hwang, Eui-kyung;Kim, Soon-bok;Kang, Shin-seuk;Kim, Ok-kyung
    • Korean Journal of Veterinary Research
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    • v.40 no.4
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    • pp.719-727
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    • 2000
  • A study was conducted to determine the susceptibility of swine to Korean foot-and-mouth disease virus (FMDV; subtype O, isolated from Chungju province) in April, 2ooo. One holstein cow was inoculated intradermolingually with suspension of homogenized tissue from a Korean native cow naturally infected with Korean FMDY. Infected cow was housed with one susceptible cow and one susceptible pig (contact sentinels). Four additional susceptible pigs were housed in the same room but caged separately (non-contacted sentinels). The contacted pig and cow as well as non-contact pigs developed typical clinical signs after 2, 3, and 7 days post exposure, respectively. We compared neutralizing antibody from the animals to FMDV $O_1$ Lombardy, O Taiwan, $O_1$ Campos, and $O_1$ Manisa after 0, 4, 7, 10, 14, 21, 28 days post challenge and post-exposure. The highest viral neutralization titer could be interpreted that serotype O Korea (Chungju isolate) is antigenically more related to $O_1$ Manisa. In addition, immunohistochemistry was used to further characterize Korean FMDV from tissues of infected pigs. Korean FMDV antigen was observed in the tongue, hoof, esophagus, and tonsil tissues of sentinel pigs. These findings suggest that Korean FMD virus isolated from cattle can be rapidly transmitted to pigs both directly and indirectly contrast field observation in which only cattle were clinically ill.

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Screening of saline tolerant plants and development of biological monitoring technique for saline stress . 1. Survey of vegetation in saline region and determination of saline tolerance of the plant species of the region. (내염성 식물의 탐색 및 생물학적 염해 모니터링 기술의 개발 1. 염해지 식생분석 및 식물종의 내염성 평가)

  • Kang, Byeung-Hoa;Shim, Sang-In
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.26-33
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    • 1998
  • This experiment was conducted to classify the plant species occurring in the saline reclaimed land by saline tolerance. The vegetation of reclaimed land was composed of various plant species from halophyte to glycophyte showing different saline tolerances. In the investigated saline region, reclaimed land of Youngjong island, Inchun city, 175 species belonging to 32 families were found. Our survey was carried out in two region, having different salinity due to different desalinization. The electricalconductivity (EC) of more saline region showed 48.0mS/cm nd the other region showed 13.0mS/cm. It is assumed that intensity of precipitation and runoff of rainfall may cause salinity gradient in the investigated region. The plant species occurred in the experimental region were classified as 72 species of annual, 42 species of biennial, and 61 species of perennial according to life cycle. For knowing relationship between vegetation of saline region and saline tolerance of occurring species, we tested the saline susceptibility of plant species collected at the saline regions. Testing plants were cultured by nutrient solution containing 200 mM NaCl, the critical concentration of survival in glycophytes. The saline tolerance was graded by the growing capacity in the sand-culture system. The more saline-tolerant species screened by sand culture were Atriplex gmelini, Suaeda asparagoides, Aster tripolium, Suaeda maritima, Salicornia herbacea, and Suaeda japonica. The most saline tolerant family was Chenopodiaceae. Poaceae, Cyperaceae, and Brassicaceae showed relatively high tolerance to saline stress. In the course of growth under the high saline condition, the most noticeable change was the darkening of leaves by increasing of chlorophyll content. The chlorophyll contents were increased with saline stress in most species.

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A Study on the Sea-sickness Susceptibility of Seafarer at the Wheel House and Engine Room (조타실과 기관실 근무자의 뱃멀미 민감성에 대한 연구)

  • Kim, Deug-Bong;Kim, Bu-Gi;Rim, Geung-Su;Kim, Hong-Ryoel;Kim, Chang-Su
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.20 no.1
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    • pp.42-48
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    • 2014
  • Seasickness not only makes persons on board vessels to vomit but also causes vertigo, headache, sleepiness, fatigue, lethargy and other discomforts. This ailment leads to disturbance of biorhythm and decline of perception which would eventually cause reduction of situational awareness among ship's operators that leads to marine accident. This study is about the sensitivity of people onboard ships to seasickness and focused on deck or navigation officer cadets(apprentice officers) and engine officer cadets(apprentice engineers) who have no previous experiences on board. It is conducted by using motion sensor that can measure ship's X, Y, Z-axis motions and through the questionnaire survey, and evaluated each students' degree of seasickness symptoms. Through this study, in same circumstance, we have known that there are different degrees of motion sickness for wheel house worker and engine room worker, It also confirmed that seasickness have high relationship with degree of hull motion and also, with cycle of hull motion. In addition, we have confirmed that Z-axis hull movement has higher relationship with seasickness than X-axis and Y-axis hull movements. This study aims to initiate additional researches about X-axis and Y-axis of the ship's motion which it expects to greatly enhance safety of wheelhouse and engine room personnel, ship's livability and comfortable sailing.

Development of Herbicide(Paraquat) Tolerant Plant Through Tissue Culture- 1. Mechanism of Plant Tolerance to Paraquat (농약(제초제)(農藥(除草劑)) Paraquat에 대한 저항성(抵抗性) 식물체(植物體) 선발육성(選拔育成)- 제1보(第1報) Paraquat에 대한 식물(植物)의 내성기작(耐性機作))

  • Kim, K.U.;Kim, D.U.;Kwon, S.T.
    • Korean Journal of Weed Science
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    • v.6 no.2
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    • pp.191-200
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    • 1986
  • The study was conducted to screen paraquat-tolerant plant species among crops and weeds, using the response of plant like leaf disc discoloration, visual injury and dry weight in the presence of paraquat. Mechanism of paraquat-tolerance was investigated in strains of soybean through evaluating activities of superoxide dismutase and peroxidase and the multiplication of callus derived from soybean cotyledon. In crops, Kwanggyo has been selected as a paraquat-tolerant variety among soybean cultivars tested, and Hood as a susceptible one. In weeds, Polygonum aviculare, Chenopodium album and Pinellia ternata were evaluated as the paraquat resistant species, providing the possibility for the donor plant species for paraquat resistance. Activity of superoxide dismutase known to detoxify paraquat was markedly greater in Kwanggyo, a paraquat-tolerant cultivar than in Hood, a susceptible one. In addition, the similar response like superoxide dismutase was observed in peroxidese activity. The greater inhibition of callus multiplication was determined in Hood, a susceptible one than a tolerant one, Kwnggyo. Based on all the informations, it is strongly proposed that paraquat tolerance in soybean is due to destruction of $O_2^-$ by elevated concentration of superoxide dismutase in the tolerant cultivar.

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An Edible Alginate Microcapsulation of Entomopathogenic Nematode, Steinernema carpocapsae (알지닌캡슐을 이용한 곤충병원선충(Steinernema carpocapsae)의 섭식유도형 제제화 기술)

  • 김용균;이승화;유용만;한상찬
    • Korean journal of applied entomology
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    • v.42 no.2
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    • pp.145-152
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    • 2003
  • Field application of the entomopathogenic nematode, Steinernema carpncapsae, is limited by its susceptibility to UV irradiation and desiccation especially at leaf spray control. This study was conducted to develop the control technique using alginate biocapsulation of the nematodes against the beet armyworm, Spodoprera exigua and the tobacco cutworm, Sp. litura that are normally infesting hosts above ground level. The alginate capsules including infective juveniles gave significant feeding toxicities to the larvae of the two lepidopteran species. The lethality followed a typical sigmoid dose-mortality pattern with increase of the nematode densities embedded in the capsules. Moisture content in the capsule was critical to the survival of the infective juveniles. More than 80% nematodes could survive above 10% moisture content remained in the capsule. Remaining moisture content within the capsule was dependent on relative humidity, ambient temperature, and capsule size, but not on citric acid reaction time during capsule formation. More than 80% of infective juveniles in the alginate capsules could survive in distilled water at 15$^{\circ}C$ for 60 days. When these nematode capsules containing welsh onion extract as another phagostimulant were applied on the 3rd instar larvae of Sp. exigua infesting peanut plants, they resulted in about 90% control efficacy. These results indicate that the alginate capsulation can be used for leaf-spray agent of the entomopathogenic nematodes as well as for improved storage purpose.

Analysis of Characteristics of Landslide Susceptibility in Rugged Mountain Range in the Korean National Park (산악형 국립공원지역의 산사태 발생과 취약지역 특성 분석)

  • Lee, Sung-Jae;Lee, Eun-Jai;Ma, Ho-Seop
    • Journal of Korean Society of Forest Science
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    • v.108 no.4
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    • pp.552-561
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    • 2019
  • In korea, debris-flow disasters are induced by typhoon and localized torrential rainfall annually. These disasters are particularly severe in the Korean national park due to its geomorphological characteristics. This study was conducted to analyze the landslide characteristics and forest environmental factors of landslide areas located in rugged mountain range in the Korean national park (Mt. Seorak, Mt. Jiri, and Mt. Sobaek). Overall, landslides occurred at 474 sites. The average area of the landslide scar among these sites was 1,212 ㎡. The average landslide sediment was 1,389 ㎥, average landslide length was 75 m, and the average width was 12.9 m. The landslides frequently occurred in regions with igneous rock and coniferous forest. In addition, slope gradient degree (31°-40°), slope gradient direction (N), vertical slope (concave), cross slope (concave), altitude (401-800 m), position (middle), stream order (first order), forest type (mixed), parent rock (igneous), and soil depth (<46 cm). The relationship between landslide soil volume and environmental factors showed positive correlation. The variables of vertical slope (complex), altitude (<1,201 m), and soil depth (<46 cm) correlated significantly at 1 % level.

Quality Changes in Mushrooms (Agaricus bisporus) due to Their Packaging Materials during Their Storage (포장재에 따른 양송이버섯의 저장 중 품질변화)

  • Lee, Da-Uhm;Chang, Min-Sun;Cho, Sun-Duk;Jhune, Chang-Sung;Kim, Gun-Hee
    • Food Science and Preservation
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    • v.20 no.1
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    • pp.7-13
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    • 2013
  • Mushrooms have a shorter shelf-life than most vegetables because of their very high respiration rates, sensitivity to enzymatic browning and susceptibility to microbial spoilage. This study was conducted to investigate effects of various packaging materials and precooling on the quality of mushrooms (Agaricus bisporus). Mushrooms were precooled at $4^{\circ}C$ for three hours and packaged using the following packaging materials; 1) polyethylene (PE) film bags of 0.03 mm thickness, 2) polypropylene (PP) film bags of 0.03 mm thickness, and 3) polystyrene (PS) tray+polyvinyl chloride (PVC) wrapper. The physiological changes (weight loss, gas composition, color, firmness, and sensory evaluation) associated with postharvest deterioration were monitored for 17 days at $10^{\circ}C$. The results showed that the PP film bag maintained quality of mushrooms most effectively, especially PP film bags inhibited decreasing firmness. The samples also exhibited smaller decreases in weight loss rate (0.57%) and Hunter L value (84.44) than PS tray+PVC wrapper (7.73%, 82.19) and PE film bags (0.89%, 82.96). Sensory evaluation level in all samples remained relatively constant during the first 5 days of storage. However, PE film bags and PS tray+PVC wrapper showed lower score of flavor, texture and color than PP film bags after 8 days of storage. This study suggested that PP film bag packaging effectively extends shelf-life of mushrooms during storage.