• 한국어병학회지
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• 제26권3호
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• pp.193-206
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• 2013

피조개, Scapharca broughtonii는 돌조개 과에 속하는 이매패류로 한국과 일본에서 매우 중요한 수산식품이며, 주로 국내 남해안 지역에서 양식되고 있다. 그러나 피조개 생산량은 여름철 대량폐사로 인하여 지난 10년 동안 급격하게 감소하였다. 대량폐사로 인한 경제적인 손실을 해결하기 위하여 피조개의 생리에 영향을 미치는 다양한 환경 인자에 대한 연구가 집중적으로 수행되어 왔으나 미생물학적인 연구는 미진한 실정이다. 본 연구에서는 피조개의 주요 혈액 성분인 헤모글로빈이 미생물의 병원성인자에 의하여 피해를 받아 발생하는 용혈현상과 피조개의 폐사와의 연관성을 규명하기 위하여 피조개 양식장의 용혈활성 미생물을 중심으로 분석하였다. 여름철 고수온기인 6월부터 9월까지 강진만과 진해만 피조개 양식장의 해저퇴적물과 피조개 체내에서 분리한 약 4,200여점의 배양가능 용혈성 미생물의 다양성 및 지역 특이적인 미생물을 분석하였다. 6월에 25속 118종, 7월에 24 속 89종, 8월에 30속 109종, 그리고 39속 141종의 미생물을 분리하였으며, 16S rDNA 정보를 통하여 지역별 미생물 다양성을 분석한 결과 폐사빈발 지역에서만 특이적으로 존재하는 140종의 미생물을 분리하였다. 이러한 지역 특이적 미생물 균총 연구는 피조개 대량폐사 원인 규명의 기초자료로 활용될 수 있을 것으로 사료되며 나아가 다양한 패류의 폐사연구에도 기여할 것으로 기대된다.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제6권10호
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• pp.465-472
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• 2017

과거에는 전력데이터를 분석하는 기법으로 주로 기계학습의 지도학습 기법을 많이 활용하였고 데이터 마이닝 기법을 통한 패턴 검출을 주로 연구하였다. 그러나 전력데이터의 규모 커지고 실시간 데이터 공급이 가능해진 현재에는 과거의 데이터 분류 및 분석 기법을 통한 데이터 분석 연구는 한계가 존재한다. 이에 본 논문에서는 큰 규모의 전력데이터를 분석할 수 있는 클러스터링 아키텍처를 제안한다. 제안하는 클러스터링 프로세스는 비지도학습기법인 K-means 알고리즘의 문제점을 보완하고 전력데이터 수집과 분석까지의 모든 과정을 자동화할 수 있는 프로세스이다. 총 3 Level로 구분하여 Row Data Level, Clustering Level, User Interface Level로 구분하여 전력데이터를 분류 및 분석한다. 또한 클러스터링의 효율성 향상을 위하여 주성분분석 및 정규분포기반의 최적의 클러스터 수 K값 추출과 이상점으로 분류되는 데이터 감소를 위한 변형된 K-means 알고리즘을 제시한다.

• 한국염색가공학회지
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• 제31권3호
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• pp.195-213
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• 2019

The purpose of this study was to identify novel extracts of brawn algae with the high deodorant capacity and antioxidant activity. To achieves this, an composition, deodorant performance and free radical scavenging activity were analyzed in powder and water extracts of Ecklonia cava(PEC/EEC), Saccharina japonica(PSJ/ESJ), Eisenia bicyclis(PEB/EEB), Sargassum spp.(PSS/ESS) and Hizikia fusiformis(PHF/EHF) using several analytical methods. Major component of five powders was verified as PEC, phlorotannin; PSJ, alginic acid and glutamic acid; PEB, carotenoid, chlorophyll, phlorotannin, tocopherol and fucoxantin; PSS, ${\beta}$-carotene and polyphenol; PHF, tannin. The highest level of DPPH scavenging activity was detected in EEC($IC_{50}=23.018{\mu}g/ml$), followed by EEB, ESJ, EHF and ESS. The deodorizing performance to ammonia was maintained high level in PEC, PSJ, PEB and PSS, while PHF showed low level in the same condition. But, the deodorizing performance to acetic acid was slightly higher in PSJ and PEC than those of PEB, PSS and PHF. Based on the results of DPPH scavenging activity and deodorizing capacity, we selected fractions of S. japonica as leading extracts and further prepared the 70% acetone extract (AESJ) and 70% ethanol extract (EESJ) from PSJ. The contents of total flavonoid, total phenol and total condensed tannin was significantly increased with 112-209% in AESJ and EESJ compared to those of ESJ. Also, a similar enhancement was observed on the level of DPPH scavenging activity and deodorizing performance against ammonia/acetic acid in AESJ and EESJ. Therefore, these results suggest that AESJ and EESJ contained with high total phenol and total condensed tannin contents may exhibit high deodorant capacity and antioxidant activity as well as has the potential for use as a powerful biomaterial for antibacterial deodorant.

• 한국구조물진단유지관리공학회 논문집
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• 제25권6호
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• pp.19-26
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• 2021

교량 받침장치부는 상부구조의 수직하중을 지지하여 하부구조에 전달하고, 교량의 붕괴사고를 방지하는 역할을 한다. 하지만, 포항지진에 의하여 총 12개 도로교량의 받침장치 몰탈 파손, 받침장치 파손 및 쐐기 손상이 보고되었다. 본 연구에서는 지진시 교량 받침장치부의 구조 시스템 특성을 고려하여 교각의 코핑부와 무수축몰탈을 포함한 면진받침 장치 실험체의 전단 거동특성 및 손상모드를 평가하였다. 받침장치 쐐기에 대한 영향을 확인하기 위하여 쐐기 설치 유무를 변수로 설정하였으며, 압축-전단 실험을 실시하여 면진장치의 전단 거동특성과 손상모드를 확인하였다. 또한 유한요소해석을 통하여 받침장치의 거동특성 및 각 구성요소별 손상원인을 분석하였다. 실험결과, 쐐기의 충돌 및 손상 발생 이후 급격한 하중변화가 발생하였으며, 받침장치와 무수축몰탈 경계부를 따라 균열이 발생하였다. 쐐기 유무에 따른 쐐기, 앵커 소켓 및 무수축볼탈의 응력거동을 비교함으로써 지진시 교량받침장치부의 손상원인을 규명하였다.

• 헤리티지:역사와 과학
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• 제37권
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• pp.349-369
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• 2004

20세기에 들어와 컴퓨터의 발달로 문화재를 복원하는데 있어 다양한 자연과학적 방법이 시도되고 있다. 문화재에 적용되는 통계학을 비롯한 자연과학적인 방법을 통해 문화재의 재질에 대한 주성분 및 미량성분을 분석하여 산지추정을 할 수 있고, 더 나아가 고대의 교역 및 교류관계를 규명하고, 생산지와 소비지 사이의 정치 경제 문화관계 등 다양한 정보를 밝혀내고 있어 그 필요성이 더욱 요구되고 있다. 본 연구는 흡수율 및 광물결정분석, 편광현미경분석, 통계분석 등의 자연과학적인 방법을 이용하여 경기도 하남시 천왕사지 유적내 기와 가마로 추정되는 소성유구의 성격과 유구 및 주변 건물지에서 출토된 기와들에 대해 상호관계를 밝히고자 시도되었으며, 연구결과 다음과 같은 결론을 얻었다. 경질 기와 편들을 제외한 연질 기와 편들은 다른 기와에서 볼 수 있는 흡수율(10%이상)과 비슷한 수치를 보였으며, 흡수율이 낮은 일부시료에서는 Mullite, Tridymite, Cristobalite와 같은 고온 결정상 등이 존재하는 것으로 보아 다른 기와편에 비해 소성온도가 높았음을 알 수 있었다. 또한 미량성분을 통한 통계분석결과 소성유구와 천왕사지 내에서 출토된 기와는 한 군으로 분류된 것으로 보아, 밀접한 관련성을 가지고 있음을 알 수 있었고, 천왕사 축조시 원거리에서 기와를 공급받은 것이 아닌 사지 내에 가마를 설치한 후 공급하였음을 알 수 있었다.

• 환경분석과 독성보건
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• 제21권4호
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• pp.220-228
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• 2018

$PM_{2.5}$-bound mercury (PBM) was monitored at weekly intervals for three years (from 2014 to 2016) at an urban (Seoul) and rural site (Baengnyeong) in South Korea. The average PBM concentrations in $PM_{2.5}$ samples over the entire sampling period were $12{\pm}11pg/m^3$ and $36{\pm}34pg/m^3$ for Baengnyeong and Seoul, respectively. Seasonal differences were pronounced, with concentrations being highest in winter due to local meteorological conditions (high gas-particle coefficient due to low temperature and low mixing layer height in winter) as well as seasonal factors, such as coal combustion for heating purposes in China. In Baengnyeong, the significant positive correlation of PBM with $PM_{2.5}$, air pollutants, and heavy metals suggested that coal combustion in China might be the most important source of ambient mercury in Korea. In winter, no correlation of PBM with $PM_{2.5}$, air pollutants, and heavy metals was seen in Seoul. Furthermore, Seoul showed higher $PBM/PM_{2.5}$ and $Pb/PM_{2.5}$ ratios in winter due to the strong atmospheric oxidation-reduction reaction conditions as well as local and regional PBM sources. We conclude that immediate attention must be given to addressing PBM levels in Korea, including considering it as a key component of future air quality monitoring activities and mitigation measures.

• 대한한의학회지
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• 제43권2호
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• pp.1-7
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• 2022

Objectives: Bee venom (BV) is a widely used therapy in Traditional East Asian Medicine (TEAM). We previously reported that BV was clinically effective for treating Parkinson's disease, that phospholipase A₂ (PLA₂) was the main component of BV, and that it induced regulatory T cells (Tregs) by binding CD206 on dendritic cells (DCs). Therefore, we aimed to reconfirm our findings in human blood samples and investigate the relationship between CD206⁺ DCs and clinical syndrome differentiation in TEAM. Methods: We surveyed 100 subjects with questionnaires on cold-heat patternization and obtained their blood samples. The obtained human peripheral blood monocytes (hPBMCs) were washed with phosphate-buffered saline (PBS). After resuspension with ex vivo media, numbers of cells were counted. Tregs were counted after culturing the samples in a 37℃ CO₂ incubator for 72 h. Results: We divided the subjects into a relatively high CD206⁺ group or a relatively low CD206⁺ group. The heat factor scores of high CD206⁺ group were significantly higher than that of low CD206⁺ group (high vs low: 239.2 ± 54.1 vs 208.4 ± 55.1, p=0.023). After culturing with PLA₂, Tregs increased in the high CD206⁺ group but decreased in the low CD206⁺ group. Conclusion: In this study, we reconfirm that CD206⁺ DCs induced Treg differentiation by incubating human blood samples with PLA₂ and that they showed an association with syndrome differentiation, especially with heat patterns, in TEAM. A heat pattern in TEAM might be one indication for PLA₂ therapy because its score was elevated in the high CD206⁺ group.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.33-33
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• 2020

Watermelon (Citrullus lanatus) is a non-seasonal, economically important, cucurbit cultivated throughout the world with Asia as a continent contributing the most. As part of the effort in diversifying watermelon genetic resources in the already cultivated group, this study was devoted to providing baseline data on morphological quality traits and health-beneficial phytonutrients of watermelon germplasm collections, thereby promoting watermelon research and cultivation programs. To this end, we reported morphological traits, citrulline, and arginine levels of watermelon genetic resources obtained from the gene bank of Agrobiodiversity Center, Republic of Korea, and discussed the relationship between each other. Diverse characteristics were observed among many of the traits. But, most of the genetic resources (>90%) were either red or pink-fleshed. Korean origin fruits contained intermediate levels of soluble solid content (SSC) while The USA, Russian, Tajikistan, Turkmenistan, Taiwan, and Uruguay originated had generally the highest levels of soluble solids. The citrulline and arginine contents using HPLC method were ranged from 6.9 to 52.1 mg/g (average, 27.3 mg/g) and 1.8 to 21.3 mg/g (average, 9.8 mg/g), respectively. The citrulline content determined using Citrulline Assay Kit was ranged from 6.5 to 42.8 mg/g (average, 27.0 mg/g). Resources with high citrulline and arginine levels contained low SSC. Whereas, red- and pink-colored flesh samples had less citrulline compared to yellow and orange. In addition to the profiling of morphological characters and phytonutrients, molecular marker characterization and identification of sources of resistance to diseases and pests are recommended for a more complete diversity analysis of watermelon genetic resources.

• Animal Bioscience
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• 제37권7호
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• pp.1168-1176
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• 2024

Objective: As a charismatic species, cashmere goats have rich genetic resources. In the Inner Mongolia Autonomous Region, there are three cashmere goat varieties named and approved by the state. These goats are renowned for their high cashmere production and superior cashmere quality. Therefore, it is vitally important to protect their genetic resources as they will serve as breeding material for developing new varieties in the future. Methods: Three breeds including Inner Mongolia cashmere goats (IMCG), Hanshan White cashmere goats (HS), and Ujimqin white cashmere goats (WZMQ) were studied. IMCG were of three types: Aerbas (AEBS), Erlangshan (ELS), and Alashan (ALS). Nine DNA samples were collected for each population, and they were genomically re-sequenced to obtain high-depth data. The genetic diversity parameters of each population were estimated to determine selection intensity. Principal component analysis, phylogenetic tree construction and genetic differentiation parameter estimation were performed to determine genetic relationships among populations. Results: Samples from the 45 individuals from the five goat populations were sequenced, and 30,601,671 raw single nucleotide polymorphisms (SNPs) obtained. Then, variant calling was conducted using the reference genome, and 17,214,526 SNPs were retained after quality control. Individual sequencing depth of individuals ranged from 21.13× to 46.18×, with an average of 28.5×. In the AEBS, locus polymorphism (79.28) and expected heterozygosity (0.2554) proportions were the lowest, and the homologous consistency ratio (0.1021) and average inbreeding coefficient (0.1348) were the highest, indicating that this population had strong selection intensity. Conversely, ALS and WZMQ selection intensity was relatively low. Genetic distance between HS and the other four populations was relatively high, and genetic exchange existed among the other four populations. Conclusion: The Inner Mongolia cashmere goat (AEBS type) population has a relatively high selection intensity and a low genetic diversity. The IMCG (ALS type) and WZMQ populations had relatively low selection intensity and high genetic diversity. The genetic distance between HS and the other four populations was relatively high, with a moderate degree of differentiation. Overall, these genetic variations provide a solid foundation for resource identification of Inner Mongolia Autonomous Region cashmere goats in the future.

• 방사선산업학회지
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• 제17권4호
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• pp.345-357
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• 2023

Nuclear forensics has been understood as a mendatory component in the international society for nuclear material control and non-proliferation verification. Radiochronometry of nuclear activities for nuclear forensics are decay series characteristics of nuclear materials and the Bateman equation to estimate when nuclear materials were purified and produced. Radiochronometry values have uncertainty of measurement due to the uncertainty factors in the estimation process. These uncertainties should be calculated using appropriate evaluation methods that are representative of the accuracy and reliability. The IAEA, US, and EU have been researched on radiochronometry and uncertainty of measurement, although the uncertainty calculation method using the Bateman equation is limited by the underestimation of the decay constant and the impossibility of estimating the age of more than one generation, so it is necessary to conduct uncertainty calculation research using computer simulation such as Monte Carlo method. This highlights the need for research using computational simulations, such as the Monte Carlo method, to overcome these limitations. In this study, we have analyzed mathematical models and the LHS (Latin Hypercube Sampling) methods to enhance the reliability of radiochronometry which is to develop an uncertainty algorithm for nuclear material radiochronometry using Bateman Equation. We analyzed the LHS method, which can obtain effective statistical results with a small number of samples, and applied it to algorithms that are Monte Carlo methods for uncertainty calculation by computer simulation. This was implemented through the MATLAB computational software. The uncertainty calculation model using mathematical models demonstrated characteristics based on the relationship between sensitivity coefficients and radiative equilibrium. Computational simulation random sampling showed characteristics dependent on random sampling methods, sampling iteration counts, and the probability distribution of uncertainty factors. For validation, we compared models from various international organizations, mathematical models, and the Monte Carlo method. The developed algorithm was found to perform calculations at an equivalent level of accuracy compared to overseas institutions and mathematical model-based methods. To enhance usability, future research and comparisons·validations need to incorporate more complex decay chains and non-homogeneous conditions. The results of this study can serve as foundational technology in the nuclear forensics field, providing tools for the identification of signature nuclides and aiding in the research, development, comparison, and validation of related technologies.