• Journal of Plant Biotechnology
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• 제7권4호
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• pp.259-265
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• 2005

Indian citrus ringspot virus (ICRSV)-free plants of Kinnow mandarin (Citrus nobilis Lour x C. deliciosa Tenora) were raised from virus-infected plants using unfertilised ovules as explants. Plants were tested by indirect ELISA and RT-PCR before using their explant. An amplified product of 539 bp was obtained by RT- PCR in ICRSV infected plants. Unfertilized ovules were excised from unopened flower buds of plants tested postive for virus and were cultured on Murashige and Skoog's (MS) basal medium supplemented with various concentrations of kinetin (KN) or malt extract (ME). Maximum induction (31.94%) of embryogenic callus was observed on MS medium supplemented with KN ($9.29\;{\mu}M$). Transfer of embryogenic calli to similar media composition resulted in somatic embryogenesis in all cultures, with an average number of 60.36 globular, 17.39 heart and 7.71 cotyledonary-shaped somatic embryos per culture. All cotyledonary shaped embryos developed into complete plantlets within 60 days on transfer to similar medium. Embryogenic callus induction, somatic embryo formation, maturation, germination and plantlet formation were achieved on MS medium supplemented with KN ($9.29\;{\mu}M$) alone. The plantlets derived from somatic embryos were transferred to sterilized soil, sand and vermiculite (3:1:1) mixture. After acclimatization, the plantlets were transferred to screen house and were indexed for ICRSV employing indirect ELISA and RT-PCR and found free of virus. A distinct feature of this study is the induction of somatic embryogenesis from unfertilised ovules to produce virus-free plants.

• 대한약침학회지
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• 제17권2호
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• pp.73-79
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• 2014

Objectives: This study was performed to analyze a 13-week repeated dose toxicity test of Sweet Bee Venom (SBV) extracted from bee venom and administered in Sprague-Dawley (SD) rats. Methods: Male and female 5-week-old SD rats were treated once daily with SBV (high-dosage group: 0.28 mg/kg; medium-dosage group: 0.14 mg/kg; or low-dosage group: 0.07 mg/kg) for 13 weeks. Normal saline was administered to the control group in a similar manner (0.2 mL/kg). We conducted clinical observations, body weight measurements, ophthalmic examinations, urinalyses, hematology and biochemistry tests, and histological observations using hematoxylin and eosin (H&E) staining to identify any abnormalities caused by the SBV treatment. Results: During this study, no mortality was observed in any of the experimental groups. Hyperemia and a movement disorder were observed around the area of in all groups that received SBV treatment, with a higher occurrence in rats treated with a higher dosage. Male rats receiving in the high-dosage group showed a significant decrease in weight during the treatment period. Compared to the control group, no significant changes in the ophthalmic parameters, the urine analyses, the complete blood cell count (CBC), and the biochemistry in the groups treated with SBV. Compared to the control group, some changes in organ weights were observed in the medium-and the high-dosage groups, but the low-dosage group showed no significant changes. Histological examination of thigh muscle indicated cell infiltration, inflammation, degeneration, and necrosis of muscle fiber, as well as fibrosis, in both the medium- and the high-dosage groups. Fatty liver change was observed in the periportal area of rats receiving medium and high dosages of SBV. No other organ abnormalities were observed. Conclusion: Our findings suggest that the No Observed Adverse Effect Level (NOAEL) of SBV is approximately 0.07 mg/kg in male and female SD rats.

• 한국균학회지
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• 제13권4호
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• pp.235-241
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• 1985

본 연구는, catabolic repression시킨 효모세포를 완전배지와 최소배지에서 derepression시켜, 배양시기 및 인산 첨가농도(free, limited, sufficient)에 따른 5종의 다당류 합성변화를 조사하였다. 그리고 다당류 합성과 무기폴리인산 축적량 및 인지질 합성 사이의 상관지수를 구하여 합성시 관련되는 유의한 정도를 검정하였다. 그 결과, 최소배지에서 catabolic derepression시킨 효모세포가, 완전배지에서 derepression시킨 세포에 비하여, glycogen의 합성이 발리 그리고 많이 일어났고, acid soluble glycogen type이 주된 함량을 나타내었으며, alkali soluble glycogen은 당이 많이 소모된 24시간 배양 후에 소량 나타났다. 무기인산 첨가정도에 따라 total glycogen합성이 일정한 비율로 빨리 그리고 높게 일어났다. Glucan의 합성에는 ALPase 중 ALPase "C"가 관련할 것으로 추정되었다. Mannan은 ezponential phase초기와 정체기때, acid soluble 분획은 정체기때 최대함량을 나타내었다. Mannan 합성과 poly-P "C"축적량 사이의 상관지수는 0.866, mannan합성과 인지질 사이의 상관지수는 0.726으로 나타나 매우 유의하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권1호
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• pp.40-46
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• 2005

Clostridium bifermentans strain DPH-1 has already been found to dechlorinate perchloroethylene (PCE) to cis-dichloroethylene (cis-DCE) via trichloroethylene (TCE). In this study, our investigation on different culture conditions of this DPH-1 strain was extended to find a more efficient and cost effective growth medium composition for this DPH-1 strain in bioremediation practices. Temperature dependency of strain DPH-1 showed that the growth starting time and PCE degradation at $15^{\circ}C$ was very slow compared to that of $30^{\circ}C$, but complete PCE degradation occurred in both cases. For the proper utilization of strain DPH-1 in more cost effective bioremediation practices, a simpler composition of an effective media was studied. One component of the culture medium, yeast extract, had been substituted by molasses, which served as a good source of electron donor. The DPH-1 strain in the medium containing molasses, in the presence of $K_{2}HPO_4\;and\;KH_{2}PO_4$, showed identical bacterial multiplication (0.135 mg protein $mL^{-1}h^{-1}$) and PCE degradation rates ($0.38\;{\mu}M/h$) to those of the yeast extract containing medium.

• 중환자간호학회지
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• 제12권2호
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• pp.85-96
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• 2019

Purpose : The purpose of this study was to examine the mediating effect of perceptions regarding the importance of patient safety management in the relationship between incident reporting attitudes and patient safety care activities for nurses in small-and medium-sized general hospitals. The objective was to provide a basis for planning tailored training programs aimed at improving patient safety care activities. Methods : This study was conducted with 187 participants in small- and medium-sized general hospitals in K city in South Korea from March 15 to March 31, 2019. The data collected from participants were analyzed using descriptive statistics, a t-test, ANOVA, Pearson's correlation coefficients, and a multiple regression using IBM SPSS/WIN 21.0 software. Results : Patient safety care activities were found to be correlated with incident reporting attitudes (r=.27, p < .001) and perceptions of the importance of patient safety management (r=.59, p < .001). Further, perceptions of the importance of patient safety management had a complete mediating effect (${\beta}=.409$, p < .001) on the relationship between incident reporting attitudes and patient safety care activities. Conclusion : Based on the findings of this study, tailored training programs regarding patient safety care activities focused on boosting perceptions of the importance of patient safety management are highly recommended to improve nurses' patient safety care activities in small- and medium-sized general hospitals.

• 문화기술의 융합
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• 제9권2호
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• pp.251-259
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• 2023

본 연구는 성인대학생의 사회적지지, 대학교육서비스품질, 학습몰입의 관계에서 학습동기의 매개효과를 검증하여 성인대학생의 학습효과 향상과 학습촉진 강화를 위한 학습체계 구축에 기초자료를 제공하고자 하였다. 이를 위하여 대구광역시와 경상북도에 위치한 2~3년제와 4년제 대학교에 재학 중인 성인대학생 573명을 대상으로 설문조사를 실시하였다. 분석한 연구결과는 다음과 같다. 첫째, 사회적지지와 학습몰입의 관계에서 학습동기는 부분 매개효과가 있는 것으로 나타났다. 둘째, 대학교육서비스품질과 학습몰입의 관계에서 학습동기는 완전 매개효과가 있는 것으로 나타났다. 이상의 연구결과는 성인대학생의 학습몰입을 높이기 위해서는 다양한 지지체계 구축과 교육서비스 향상을 위한 지속적인 개발과 노력이 필요하다는 것을 시사한다.

• 벤처창업연구
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• 제15권5호
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• pp.65-76
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• 2020

본 연구는 여성의 셀프리더쉽이 창업이라는 새로운 환경에 적응하는 것이 중요하다는 점을 반영하여 여성의 셀프리더쉽이 자기효능감을 매개로 하여 창업의도에 미치는 영향을 분석하였다. 특히, 영향요인인 셀프리더쉽을 보다 구체적으로 파악하기 위하여 셀프리더쉽 전체와 더불어 하위요인인 행동지연 전략, 보상자연 전략 및 건설적 사고전략에 대해서도 검증하였다. 연구방법으로는 온라인 설문에 응답한 198명을 대상으로 Hayes(2018)가 제안한 PROCESS macro의 model 4번 모델을 이용하여 분석하였으며, 간접효과는 붓스트랩 샘플을 5,000개로 지정하여 분석하였다. 연구결과는 다음과 같다. 첫째, 자기효능감은 여성의 셀프리더쉽이 창업의도에 영향을 미치는 매개효과가 있는 것으로 나타났다. 이때 매개효과는 부분매개로 나타났다. 둘째, 자기효능감은 여성의 행동지향적 전략이 창업의도에 영향을 미치는 매개효과가 있는 것으로 나타났다. 이때 매개효과는 부분매개로 나타났다. 셋째, 자기효능감은 여성의 자연보상적 전략이 창업의도에 영향을 미치는 매개효과가 있는 것으로 나타났다. 이때 매개효과는 부분매개로 나타났다. 넷째, 자기효능감은 여성의 건설적 사고전략이 창업의도에 영향을 미치는 매개효과가 있는 것으로 나타났다. 이때 매개효과는 완전매개로 나타났다. 이는 여성의 셀프리더쉽이 높을수록 자기효능감과 창업의도가 높아질 수 있음을 알 수 있다. 이러한 연구 결과에 따라 여성 창업에 대한 발전적 제언을 하였다.

• 한국초지조사료학회지
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• 제32권3호
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• pp.229-236
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• 2012

본 시험은 2010년부터 2012년까지 3년동안 경북 성주 계명문화대학 실험목장에서 이탈리안 라이그라스 품종의 생육특성 및 건물수량을 평가하기 위하여 실시하였다. 시험설계는 조중생종은 품종을 처리로 12처리 3반복, 중만생품종은 품종을 처리로 10처리 3반복 난괴법으로 배치하였다. 1년차 시험은 2010년 10월 12일에 파종하여 조중생종은 2011년 4월 29일, 중만생종은 동년 5월 4일에 수확하였고, 2년차시험은 2011년 10월 3일에 파종하여 조중생품종은 2012년 5월 5일 중만생종은 동년 5월 12일에 수확하였다. 조중생 품종의 건물수량은 Yeonbong3, Seongnong, Winter Hawk, Pride 및 Kowinnearly 품종이 각각 9,850, 9,778, 9,486, 9,363 및 9,267 kg/ha 순위였으며 이들 품종 간에 수량 차이는 없었으나 다른 7품종 보다 높았다(p<0.05). 중만생 품종의 건물수량은 Tetragold, Hwasan 101, Jumbo, Sungrazer, Master, SelectIV 및 KB Royal이 각각 9,542, 9,492, 9,103, 8,981, 8,903, 8,870 및 8,681 kg/ha 순위였으며 이들 품종 간에 수량 차이는 없었으나, 다른 3품종 보다는 높았다(p<0.05). 내한성은 중만생 품종중에서 Hwasan 101가 다른 품종보다 높은 경향을 보였다. 본 시험의 결과에 의하면 조기수확 시 건물수량 측면에서 조중생 품종이 유리할 것으로 생각된다.

• Steel and Composite Structures
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• 제19권1호
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• pp.237-262
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• 2015

In this study, we try to compare different intensity measures for evaluating nonlinear response of bridge structure. This paper presents seismic analytic fragility of a three-span concrete girder highway bridge. A complete detail of bridge modeling parameters and also its verification has been presented. Fragility function considers the relationship of intensities of the ground motion and probability of exceeding certain state of damage. Incremental dynamic analysis (IDA) has been subjected to the bridge from medium to strong ground motions. A suite of 20 earthquake ground motions with different range of PGAs are used in nonlinear dynamic analysis of the bridge. Complete sensitive analyses have been done on the response of bridge and also efficiency and practically of them are studied to obtain a proficient intensity measure for these types of structure by considering its sensitivity to the period of the bridge. Three dimensional finite element (FE) model of the bridge is developed and analyzed. The numerical results show that the bridge response is very sensitive to the earthquake ground motions when PGA and Sa (Ti, 5%) are used as intensity measure (IM) and also indicated that the failure probability of the bridge system is dominated by the bridge piers.

• 약학회지
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• 제36권1호
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• pp.80-86
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• 1992

Streptozotocin (STZ) is a naturally occurring nitrosoamide used extensively to produce diabetes in experimental animals. Our previous study has demonstrated that i.v. administraton of streptozotocin induces significant red blood cell hemolmysis in rats. Since it has been reported that the highest concentration of STZ is found in the liver, the effect of STZ in freshly isolated rat hepatocytes has been investigated. STZ treatment (10 mM) did not cause significant loss of viability throughout 4 hour incubation, while high dose of STZ (300 mM) to hepatocytes resulted in complete cell death within 3 hours. Addition of 40 mM glucose to incubation medium did not potentiate STZ-induced hepatotoxicity, suggesting that STZ-induced hyperglycemia in vivo did not affect its hepatotoxicity. To investigate the mechanixm of the toxicity, intracellular total glutathione level was determined. Tratment with 10 mM STZ which was not toxic to hepatocytes led to complete depletion of intracellular glutathione level within 1 hour incubation. These results suggest that STZ-induced hepatotoxicity may be independent on the intracellular glutathione depletion.