• Title/Summary/Keyword: Complete medium

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Optimum Seeding Rate in Different to Soil Salinity for Broadcasting on the Rice Flooded Paddy Surface at South-western Reclaimed Saline Land of Korea (서남부 간척지에서 벼 담수표면산파재배시 토양 염농도별 적정 파종량)

  • Back, Nam-Hyun;Choi, Weon-Young;Ko, Jong-Cheol;Park, Hong-Kyu;Nam, Jeong-Kweon;Park, Kwang-Geun;Kim, Sang-Su;Kim, Bo-Kyeong;Kim, Choung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.47-51
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    • 2006
  • This study was conducted to establish the optimum seeding rate in different soil salinity level for yield stability of broadcasting on flooded paddy surface to the reclaimed saline land of south-western part at Gyehwado substation of the Honam Agricultural Research institute in $2003{\sim}2004$. Soeganbyeo was tested in the Munpo series (fine sand loam) the results obtained is as follows: As seeding rate was higher, the number of seeding stand was increased and the number of seeding stands in the low salinity field is sharply increased than those of the medium salinity field. The length of culm in medium salinity field tends to be shorter than that of the low salinity field and as seeding rate was increased, the lodging is severe. The milled rice yield was increased as up to 9 kg/10a in low and medium salinity soil. Complete rice was no significantly increased over 5 kg/10a seeding rate in low salinity field and over 7 kg/10a seeding rate in medium salinity field. Considering the yield of milled and complete rice, seeding stand and lodging, The proper seeding rate is $5{\sim}7kg/10a$ in low salinity and $7{\sim}9kg/10a$ in medium salinity for broadcasting on flooded paddy surface at the reclaimed saline land of southwestern part.

Optimization for Mycelial Growth and Inhibitory Effect on Nitric Oxide Production of Cordyceps nutans Pat. (노린재동충하초의 배양 최적화 및 NO 생성 저해 효과)

  • Lee, Ki-Man;Lee, Geum-Seon;Nam, Sung-Hee;Lim, Sung-Cil;Kang, Tae-Jin
    • Korean journal of applied entomology
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    • v.50 no.4
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    • pp.307-314
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    • 2011
  • Cordyceps (vegetable wasp and plant worm), an entomopathogenic fungi, has been used as a herbal medicine in Asian countries since ancient times. Cordyceps nutans is common but there is little research on this species. This study investigated the optimal culture conditions of C. nutans and the inhibitory effect on nitric oxide (NO) production in RAW 264.7 cell treated culture broth. The optimal conditions for the mycelial growth were $25^{\circ}C$ and pH 7.0-8.0. Mycelial growth was highest on mushroom complete medium (MCM), V8 juice agar (V8A), and yeast malt dextrose (YMD) medium. Mycelial growth on mushroom minimal medium (MMM) did not occur, so nutrient source was essential. Dextrose and sucrose as carbon sources, and ammonium citrate as a nitrogen source were satisfactory for mycelial growth. Cytotoxicity of C. nutans culture broth was not found in RAW 264.7 cells. C. nutans culture broth suppressed NO production of lipopolysaccharide (LPS)-stimulated RAW 264.7 cell in a dose-dependent manner. Thus, our results provided the optimal conditions for cultivation of C. nutans and showed that C. nutans may have excellent physiological activities.

Growth and Differentation of Rat Mammary Epithelial Cells Cultured in Serum-free Medium

  • Kim, Dong-Yeum;Jhun, Byung-Hak;Lee, Kyung-Hee;Hong, Seung-Chul;Clifton, Kelly-H.;Kim, Nam-Deuk
    • Archives of Pharmacal Research
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    • v.20 no.4
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    • pp.297-305
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    • 1997
  • A new serum-free defined medium was developed that supports the growth of normal rat mammary epithelial cells. Mammary organoids from the glands of female F344 rats were cultured in a serum-free medium. Monolayer culture colonies developed within a week and remained viable for months in culture. Upon subculture of one-week-old primary colonies, almost the same morphology of colonies was developed. The scrape loading/dye transfer technique showed that most of colonies that developed in a serum-free medium containing EGF, human transferrin, insulin, and hydrocortisone (basal serum-free medium, BSFM) failed to show cell-cell communication. However, colonies cultured in BSFM supplemented with prolactin, $E_2$, and progesterone (complete hormone serum-free medium, CHSFM) showed cell-cell communication at 14 days of primary culture or of subculture. By flow cytometry with FITCPNA and PE-anti-Thy-1.1 monoclonal antibody, we distinguished four RMEC subpopulations in cultures in both media: Thy-1.1+ cells, PNA+ cells, cells negative to both reagents and cells positive to both reagents. It is likely that combined prolactin, cortisol, and insulin in CHSFM stimulate terminal differentiation of clonogenic cells.

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Design and Evaluation of a Contention-Based High Throughput MAC with Delay Guarantee for Infrastructured IEEE 802.11WLANs

  • Kuo, Yaw-Wen;Tsai, Tung-Lin
    • Journal of Communications and Networks
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    • v.15 no.6
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    • pp.606-613
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    • 2013
  • This paper proposes a complete solution of a contention-based medium access control in wireless local networks to provide station level quality of service guarantees in both downstream and upstream directions. The solution, based on the mature distributed coordination function protocol, includes a new fixed contention window backoff scheme, a tuning procedure to derive the optimal parameters, a super mode to mitigate the downstream bottleneck at the access point, and a simple admission control algorithm. The proposed system guarantees that the probability of the delay bound violation is below a predefined threshold. In addition, high channel utilization can be achieved at the same time. The numerical results show that the system has advantages over the traditional binary exponential backoff scheme, including efficiency and easy configuration.

A study on thermo-elastic interactions in 2D porous media with-without energy dissipation

  • Alzahrani, Faris;Abbas, Ibrahim A.
    • Steel and Composite Structures
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    • v.38 no.5
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    • pp.523-531
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    • 2021
  • The generalized thermoelastic analysis problem of a two-dimension porous medium with and without energy dissipation are obtained in the context of Green-Naghdi's (GNIII) model. The exact solutions are presented to obtain the studying fields due to the pulse heat flux that decay exponentially in the surface of porous media. By using Laplace and Fourier transform with the eigenvalues scheme, the physical quantities are analytically presented. The surface is shocked by thermal (pulse heat flux problems) and applying the traction free on its outer surfaces (mechanical boundary) through transport (diffusion) process of temperature to observe the analytical complete expression of the main physical fields. The change in volume fraction field, the variations of the displacement components, temperature and the components of stress are graphically presented. Suitable discussion and conclusions are presented.

Influence of impulsive line source and non-homogeneity on the propagation of SH-wave in an isotropic medium

  • Kakar, Rajneesh
    • Interaction and multiscale mechanics
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    • v.6 no.3
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    • pp.287-300
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    • 2013
  • In this paper, the effect of impulsive line on the propagation of shear waves in non-homogeneous elastic layer is investigated. The rigidity and density in the intermediate layer is assumed to vary quadratic as functions of depth. The dispersion equation is obtained by using the Fourier transform and Green's function technique. The study ends with the mathematical calculations for transmitted wave in the layer. These equations are in complete agreement with the classical results when the non-homogeneity parameters are neglected. Various curves are plotted to show the effects of non-homogeneities on shear waves in the intermediate layer.

Protease released during germination of dictyostelium discoideum spores (Dictyostelium discoideum의 포자가 발아중에 유출하는 protease에 관하여)

  • 윤경하;윤철호
    • Korean Journal of Microbiology
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    • v.27 no.1
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    • pp.43-47
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    • 1989
  • Characteristics and roles of protease released during the germination of Dictyostelium discoideum spores were investigated. When geat activated, the spores germinated, progressively releasing the protease into the extracellular medium. The protease activity exhibited high at pH 2.5. When cyclogeximide was added to culture, complete germination (emergence) and protease release were stopped. Addition of purified nonspecific protease to culture speeded up germination. These results suggest that excreted protease may play a role in removal of the spore wall.

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Surfactant Effect on the Hydrophobic Interaction between Rhodamine 6G and Sodium Tetraphenylborate

  • Oh, Sae-Yung;Lee, Beom-Gyu;Kim, Kang-Jin
    • Bulletin of the Korean Chemical Society
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    • v.9 no.5
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    • pp.308-311
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    • 1988
  • The hydrophobic interaction occurring between rhodamine 6G and tetraphenylborate was investigated spectroscopically by varying the medium with the addition of surfactants or ethanol. The ion aggregates formed between the two ions were destroyed by the additives. The dye existed as monomeric species in the presence of a cationic surfactant whereas it was incorporated with anionic and nonionic surfactants. For the complete dissociation more than the critical micelle concentration (cmc) was required with a nonionic surfactant while less than cmc was necessary with the others.

Enhanced apoptosis of IFN-$\gamma$ treated macrophage in a depleted nutritional state

  • Cho, Seong-Jun;Rhe, Dong-Kwon;Pyo, Suhk-Neung
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.138.2-139
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    • 2003
  • Apoptosis has been implicated as an important mediator in immunosuppression observed in a depleted nutritional state. The recent report has indicated that IFN-$\gamma$ treated bone marrow macrophages were protected from apoptosis induced by several stimuli in complete medium condition. However, our previous study demonstrated that IFN-$\gamma$ treated peritoneal macrophages were enhanced the apoposis in a depleted nutritional state. Therefor, we investigated the apoptotic regulatory mechanism of IFN-$\gamma$ in malnutrition-induced macrophage. (omitted)

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Anti-/pro-apoptotic regulatory potentials of LPS/IFN-${\gamma}$ in the mulnutrition induced macrophage

  • Cho, Seong-Jun;Rhee, Dong-Kwon;Pyo, Suhk-Neung
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.310.2-310.2
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    • 2002
  • Macrophage activated by LPS/IFN-${\gamma}$ playa important role in imflammation. innate immunity and tumor immunity. The recent report has indicated that LPS treated bone marrow macrophages were induced apoptosis. but IFN-${\gamma}$ protects from apoptosis induced by several stimuli in complete medium condition (Jordi et al.. Immunity. Vo1.11. 103-113. 1999). (omitted)

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