• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1091-1101
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• 2009

In an attempt to understand the biochemical mechanism for the synthesis of the anabolic steroid, 19-nortestosterone, produced by prepubertal boar testes and its physiological role, normalized complementary DNA (cDNA) from boar testes was generated. DNA sequencing of 2,016 randomly selected clones yielded 794,116 base pairs of high quality nucleotide sequence. Computer-assisted assembly of the nucleotide sequence of each clone resulted in 423 contigs and 403 singletons including several genes for steroidogenic enzymes and molecules related to steroid metabolism. Analysis of gene expression pattern by use of the presently-fabricated cDNA microarray identified a number of genes that were differentially expressed during the postnatal development period in boar testes. Two genes of unknown function were identified to be highly expressed in the testis of 2-weeks-old neonatal boar. In addition, the sequencing of open reading frames of these genes revealed their homology with human alpha hemoglobin and Homo sapiens hypothetical LOC643669, transcript variant 1. Moreover, the transcripts of these genes were also detected in porcine muscle and adipocytes, in addition to Leydig cells of pigs.

• Fisheries and Aquatic Sciences
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• 제19권8호
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• pp.33.1-33.7
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• 2016

Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5'-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of $0.07{\pm}0.01^{\circ}C$ at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

• 한국사회복지학
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• 제65권1호
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• pp.201-226
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• 2013

외환위기 이후 청년실업의 증가가 사회적 문제로 부각되었으나, 청년층의 노동시장 경험에 관한 연구는 제한적으로 이루어져 왔다. 본 연구에서는 노동경력을 단순한 일자리 이동과 달리 노동지위의 연속적 배열과 순서적이고 위계적인 변화과정으로 개념화하였으며, 배열분석을 활용하여 외환위기 전후 청년층의 노동경력을 노동지위의 다양한 측면에서 비교하였다. 분석 결과, 외환위기 이후 첫 일자리로의 이행기간이 장기화되고 고용형태와 사업장 규모 면에서 첫 일자리의 질적 저하가 발생하였음이 확인되었다. 또한 외환위기 이후 청년 코호트는 미취업형과 실업형, 비대기업형, 비정규직형 및 이동형 특성을 갖는 경력유형에 속할 상대적 위험률이 높았다. 이러한 결과는 전반적으로 외환위기 이후 청년층의 고용불안정성이 더 커졌으며, 내부노동시장형에 비해 외부노동시장형 경력유형이 상대적으로 증가한 것을 의미한다.

• 대한의용생체공학회:의공학회지
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• 제42권3호
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• pp.125-142
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• 2021

The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2010년도 추계학술대회
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• pp.211-213
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• 2010

본 논문에서는 직교 주파수 다중화 (OFDM) 신호의 새로운 peak to average power ratio (PAPR) 감쇄 기법을 제안한다. 제안 기법은 시간영역에서 OFDM 심볼의 동상 (in-phase) 성분 및 직교 (quadrature) 성분을 회전시키고 재조합한다. 제안 기법은 기존의 partial transmit sequence (PTS) 기법과 비교하여 계산량을 현저히 줄일 수 있는 장점이 있었음에도 모의 실험을 통해 얻어진 PAPR의 complementary cumulative distribution function (CCDF)는 PTS와 비교할 때 거의 같은 성능을 보였다. 더욱이 제안된 기법은 additive white Gaussian (AWGN) 채널 및 다중 경로 페이딩 채널 (multi-path fading channel)에서 일반적인 OFDM 신호를 전송할 때와 거의 같은 BER (bit error rate) 성능을 보였다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2004년도 ICCAS
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• pp.358-362
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• 2004

Verification and testing are complementary techniques that are used to increase the level of confidence in the correct functioning of communication systems as prescribed by their specifications. This paper presents an experience of model checking for a formal railway signaling protocol specified in LTS (Labeled Transition System). This formal approach checks deadlock, livelock and reachability for the state and action to verify whether properties expressed in modal logic are true on specifications. We also propose a formal method for semi-automated test case generation for a railway signaling protocol described in I/O FSM (Input/Output Finite State Machine). This enables the generation of more complete and consistent test sequence for conformance testing. The above functions are implemented by C++ language and included within RSPVTE (Railway Signaling Protocol Verification and Testing Environment).

• BMB Reports
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• 제37권1호
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• pp.28-34
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• 2004

The discovery of biochemical and cellular functions of unannotated gene products begins with a database search of proteins with structure/sequence homologues based on known genes. Very recently, a number of frontier groups in structural biology proposed a new paradigm to predict biological functions of an unknown protein on the basis of its three-dimensional structure on a genomic scale. Structural proteomics (genomics), a research area for structure-based functional discovery, aims to complete the protein-folding universe of all gene products in a cell. It would lead us to a complete understanding of a living organism from protein structure. Two major complementary experimental techniques, X-ray crystallography and NMR spectroscopy, combined with recently developed high throughput methods have played a central role in structural proteomics research; however, an integration of these methodologies together with comparative modeling and electron microscopy would speed up the goal for completing a full dictionary of protein folding space in the near future.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.2004-2007
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• 2006

An efficient method for the in vitro reassembly of homologous DNA sequences is presented. The proposed method involves obtaining single strands of homologous genes and hybridizing them to obtain partially hybridized heteroduplex DNA; cleaving the single-stranded regions of the heteroduplex DNA using S1 nuclease to generate double-strand DNA fragments; denaturing the double-strand DNA fragments to generate single-strand DNA fragments; conducting a series of polymerase chain reactions (PCR) using the single-strand DNA fragments as internal primers and a mixture of homologous DNA as templates to obtain elongated reassembled DNA; and finally, amplifying the reassembled DNA by a PCR using terminal primers. As a result, DNA reassembly could be achieved between homologous genes with a sequence similarity as low as 78%.

• 약학회지
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• 제32권4호
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• pp.213-221
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• 1988

Inducible MLS resistance gene of Bacillus licheniformis specified by erm K was subcloned in Bacillus subtilis and the DNA sequence corresponding to its control region was determined. The determinant erm K was in Pvu II=Hind III fragment, which was 1.3 kb. The leader region is capable of forming a complex series of inverted complementary repeat sequences (ICRS) centering on at least six axes of symmetry, some of them mutually exclusive, in a way that resulted ultimately in post-transcriptional unmasking of the ribosome loading site for methylase synthesis.

• 한국식물병리학회지
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• 제12권2호
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• pp.182-186
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• 1996

국내에서 재배되고 있는 고추(Capsicum annuum L.)로부터 분리된 TMV pepper 계통을 density gradient centrifugation을 이용하여 순화하였다. 이로부터 바이러스의 total RNA를 분리하였고 RT-PCR에 의하여 TMV pepper 계통의 외피단백질 cDNA를 합성, 증폭하였으며 이를 pBluescript II SK- 벡터에 재조합하였다. 본 실험에서 바이러스 외피단백질과 3` non-coding region을 포함하는 재조합 클론 p1561과 p1562로부터 염기서열을 분석하였고 그 결과로 477 염기의 외피단백질 유전자를 포함하는 691 염기가 합성되었음을 확인하였으며 이것과 TMV common 계통으로부터 합성된 외피단백질 cDNA와의 최대 유사도는 69%였다. 또한 유추된 아미노산 서열에서 이들 두 계통간의 최대 유사도는 81%였다.