• Bulletin of the Korean Chemical Society
• /
• v.32 no.5
• /
• pp.1569-1574
• /
• 2011

The presence of replication-competent adenovirus (RCA) subpopulations in adenoviral vector products raises a variety of safety issues for development of therapies based on gene therapy. To analyze the differing effects of adenoviral vector and RCA in vivo, we examined alterations in amino acids (AAs) using rat plasma following injection of ${\beta}$-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA. Plasma AAs were examined by gas chromatography-mass spectrometry. A total of 16 AAs were positively measured. In the rAdLacZ group compared to the control group, the level of aspartic acid was significantly increased (Student's t-test), while the level of glutamic acid was significantly reduced. Additionally, in the RCA group compared to the control group, the level of four AAs, valine, leucine, and isoleucine as branched-chain amino acids, and proline were significantly increased, whereas the levels of three AAs, glycine, threonine, and glutamic acid were significantly reduced. Altered plasma free AA metabolic patterns in rAdLacZ and RCA groups, compared with the control group, may explain the disturbance of AA metabolism related to viral infection.

• Korean Journal of Animal Reproduction
• /
• v.19 no.1
• /
• pp.35-41
• /
• 1995

In this study, we have tested whether the retrovirus vector system is applicable in transgenic cattle production. To overcome low infectivity of currently available retrovirus vector system we have directly microinjected retrovirus-producing cells into the perivitelline space of the day 1.5 embryos. The virus-producing cell line was designed to release replication-defective retrovirus encapsidated with Gibbon ape leukemia virus (GaLV) envelope protein. E. coli LacZ gene was used as a marker gene to facilitate evaluation of the transgene expression and X-gal staining at morula or blastocyst stage resulted in expression of E. coli LacZ gene The results in these experiments were summarized as follows : 1. The lowest concentration of polybrene necessary for efficient virus infection was Sf' g/ml. 2. Development rate from day 1.5 embryos microinjected with virus-producing cells to the morulae /blastocysts was 29%. 3. 21% of the morulae /blastocysts were LacZ+. 4. There was no evidence that the retrovirus-producing cells used in this study produced replication-competent retrovirus.

• Microbiology and Biotechnology Letters
• /
• v.18 no.5
• /
• pp.454-459
• /
• 1990

For the genetic multipurpose of antagonistic abilities of Pseudomom etutzeri YPL-1 aganist Fusarium solani causing root rot of many important crops by genetic engineering, optimal conditions for transformation of P-stutzeri YPL-1 by pKT230 were investigated. Maxium frequency of the transformation was achieved when cells were harvested at early exponential growth phase. The highest transformation efficiency was obtained when the competent cells were exposed to chilled transformation buffer containing 20 mM RbCI, 100 mM $CaCl_2$ and added l${\mu}g$/ml of plasmid DNA. The pH optimum for transformation was 6.5. When the bacterial cells that were incubated during 60 minutes for the competence were brought in contact with plasmid DNA, the transformations were obtained in the best frequency. It was formed that transformation frequency was 2 ~$6 \times 10^{-6}$ under the optimal conditions.

• The Journal of Korean Academic Society of Nursing Education
• /
• v.20 no.3
• /
• pp.424-434
• /
• 2014

Purpose: The aim of this study was to propose a teaching model to improve the cultural competence care of undergraduate Korean nursing students. Methods: A literature review was performed from 2000 through 2014 using internet to identify studies that presented nursing curricula and teaching-learning strategies in Korea. We analyzed retrieved Korean seven articles using Campinha-Bacote's ASKED model in combination with reflective experiences of authors observing classrooms in the U.S. Results: Six syllabuses presented information about cultural diversity nursing concepts in Korea. A traditional lecture focused on presenting contents about cultural knowledge by an instructor in the classroom was identified as a commonly used teaching strategy. We found that cultural competency related to cultural desire, awareness, and encounter was lacking. Thus, we developed teaching model based on the Korean nursing education contexts and combined with Campinha-Bacote model to improve cultural competency in nursing. Conclusions: The proposed teaching model shows promise as a strategy for improving the knowledge, attitudes, and skills related to cultural competent for undergraduate nursing students in Korea. However, evidences that this model improves nursing students' clinical practices reflecting cultural competent care are still lacking. Future research focusing on determining teaching methods and contents in clinical practices will be most effective.

• The Journal of the Korea Contents Association
• /
• v.7 no.9
• /
• pp.139-147
• /
• 2007

The police is huge career civil service organization. In 1999, police organization employed 7,801 persons as a new hiring. The police had revised employment system several times for competent human resources. But, it is true that much improvements need yet for person's employment which equip eligibility. Specially, discussions need for many new hirings by duty police system abrogation. Career civil service system is impossible of dismissal after permanent appointment. It is true that competitive rate of police officer examination much rose. But it's not important about organization development in addition this unconnects with competent resources. Must change police officer employment system for organization's future development. This study proposed policy about improvement way of examination for service step, field training officer and appointment.

• The Journal of the Korea Contents Association
• /
• v.17 no.8
• /
• pp.27-38
• /
• 2017

While the practice of investigative journalism among public service broadcasters has declined in the 2010s, Newstapa, a nonprofit online news organization, emerged as a trustworthy provider of investigative reportage. This study explores the reasons why the realistic conditions for investigative journalism -- such as competent journalists, adequate support systems, and productive cultures -- were first realized and later changed among public broadcasters and in Newstapa. Governmental pressure and the public broadcasting managements' anti-journalism policy had a chilling effect on the production staff of public broadcasters. Their enthusiasm was affected and led to a decline in the production culture of discussion and teamwork. The staff's strong will to practice journalism and the persistence of a production culture that respected autonomy and guaranteed independence enabled Newstapa to become an influential investigative journalism institution in a relatively short period of time. This proves that meaningful investigative journalism is possible when the combined elements of competent and determined journalism practitioners, as well as a system supportive of extensive news gathering and independence of production, are in place. An investigative program is the outcome of ideal broadcasters' journalistic conditions and a vibrant and thriving production culture.

• Korean Security Journal
• /
• no.62
• /
• pp.277-293
• /
• 2020

The purpose of this study is to select the talented and competent personal caregiver personnel through a professional and rational inspection system, and to provide safer personal care services to the public so that the people who are eligible for service can lead a safe life. The purpose of this study is to cultivate qualified and competent personal guardian personnel and provide professional and stable services to the people so that the people who can use the service can lead a safer life. In order to grow into a system that meets the objectives of the personal guardian qualification screening system, it must be revised and supplemented from various evaluation methods. The improvement measures related to the practical test of the system for qualifying personal guardians are as follows. First, the fitness evaluation items should be added. Second, it is necessary to reestablish the test technology. (Remove technology that causes the second threat) (Adds skills that can be used as civilian status) Third, the fallout and application action must be added. Fourth, evaluation in various starting positions is necessary. Fifth, evaluation should be made by diversifying the distance and direction with the opponent.

• Korean Journal of Social Welfare
• /
• v.61 no.4
• /
• pp.85-108
• /
• 2009

The quickening pace of international migration in the last twenty years brought fundamental demographic and social changes to the Korean society. Increasing number of marriage-based immigrant families imposes new challenges on social work community; however, the social work community has not been equipped with necessary practice models, sets of skills and professionals with cultural competency. This study aims to explore an integrative framework of social work practice with this client group. Research foci include the profile of marriage-based immigrant families in Korea, available social services at the time, challenges and issues they face, the compatibility of the empowerment based social work practice, and micro/mezzo/macro level concerns that arise for the effective service provision. Challenges among marriage-based immigrant families are converged into personal/familial, community related, and larger social-cultural issues. Empowerment is a multi-dimensional social process to help people gain control over their lives; a process that fosters power in people, for use in their lives, their communities and in their society. Empowerment based social work practice can be the most competent framework for working with multi-cultural families considering their multi-faceted acculturation issues while they navigate thru Korean society. Issues of educating and training of culturally competent social workers are discussed.

• Journal of the Korea Convergence Society
• /
• v.8 no.11
• /
• pp.257-262
• /
• 2017

The success or failure of the Korean game industry is a key issue for the creation of competent and talented human resources for the production of competent contents. The purpose of this study is to elucidate how to characterize and educate human - friendly game graphic designers with global competitiveness. The success of multi-disciplinary education will be maximized when cooperation with the responsible professors, the active will of the professor, the educational vision of the school, and real-time and active administration are supported In this study, based on the analysis of demand and supply of the human resources in the game industry, we found out that the game industry requires specialized manpower of technology-friendly game graphic designer who has the creativity, practical. application ability, and the international competency. In addition, we suggested ways of linking the curriculum with the local industries and related organizations such that they could be well educated as experts in related fields.

• Journal of Korean Academy of Nursing
• /
• v.24 no.3
• /
• pp.499-510
• /
• 1994

The purpose of this study was to Identify the self-actualization level of Junior College Nursing Students and to analyze the variables that may be related to self-actualization. A convenient sample of 346 students were selected from three colleges located in Seoul and Kyungki-Province. The data were collected using a direct survey method from September17to October 2, 1992. The instrument used for this study was the self-actualization test modified by Kim & Lee(1983). The collected data were analyzed using percentages, means, 1-test, X$^2$-test, ANOVA and Pearson’s correlation coefficient, with the SPSSPC$^{+}$ Package. The results of this study are as follows : 1) The self-actualization level of subjects was moderate. The subjects showed higher scores in the areas of Self actualizing Values and Nature of Man than the standardized normal groups. The subjects showed lower scores in the areas of Inner Directed Existentiality, Self acceptance and Capacity for Intimate Contact. 2) The subjects attending day junior colleges showed higher scores in the areas of Time competent, Existentiality and Self Acceptance than those attending college in the evening. The subjects attending evening junior colleges showed higher scores in the areas of Spontaneity than those attending college by day. 3) The junior students showed higher scores in the areas of Feel-ing Reactivity than freshman. The students with no religion showed higher scores in the areas of existentiality than those who had a religion. The group who had the experience of failure on the college entrance examination showed lower scores in the areas of Nature of Man than those without this experience. Those who chose their major voluntary showed scores higher in the areas of I nor Directed, Self Actualizing Values, Spontaneity and Self regard than the unsatisfied group. The group satisfied with college life showed higher scores in the areas of Time Competent, Inner Directed, Self Actualizing Values and Self Regard than the unsatisfied group. In conclusion self actualization level depends on motivation of choice, satisfaction with nursing as a major and satisfaction with college life. Therefore, and effective guidance program is required to improve motivation and satisfaction with nursing as a major and with college life.