• Title/Summary/Keyword: Community Identification

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Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome

  • Tan, Hao;Mooij, Marlies J.;Barret, Matthieu;Hegarty, Pardraig M.;Harrington, Catriona;Dobson, Alan D.W.;O'Gara, Fergal
    • Journal of Microbiology and Biotechnology
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    • v.24 no.1
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    • pp.113-118
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    • 2014
  • Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF.

OAPR-HOML'1: Optimal automated program repair approach based on hybrid improved grasshopper optimization and opposition learning based artificial neural network

  • MAMATHA, T.;RAMA SUBBA REDDY, B.;BINDU, C SHOBA
    • International Journal of Computer Science & Network Security
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    • v.22 no.4
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    • pp.261-273
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    • 2022
  • Over the last decade, the scientific community has been actively developing technologies for automated software bug fixes called Automated Program Repair (APR). Several APR techniques have recently been proposed to effectively address multiple classroom programming errors. However, little attention has been paid to the advances in effective APR techniques for software bugs that are widely occurring during the software life cycle maintenance phase. To further enhance the concept of software testing and debugging, we recommend an optimized automated software repair approach based on hybrid technology (OAPR-HOML'1). The first contribution of the proposed OAPR-HOML'1 technique is to introduce an improved grasshopper optimization (IGO) algorithm for fault location identification in the given test projects. Then, we illustrate an opposition learning based artificial neural network (OL-ANN) technique to select AST node-level transformation schemas to create the sketches which provide automated program repair for those faulty projects. Finally, the OAPR-HOML'1 is evaluated using Defects4J benchmark and the performance is compared with the modern technologies number of bugs fixed, accuracy, precession, recall and F-measure.

Current methodologies in construction of plant-pollinator network with emphasize on the application of DNA metabarcoding approach

  • Namin, Saeed Mohamadzade;Son, Minwoong;Jung, Chuleui
    • Journal of Ecology and Environment
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    • v.46 no.2
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    • pp.126-135
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    • 2022
  • Background: Pollinators are important ecological elements due to their role in the maintenance of ecosystem health, wild plant reproduction, crop production and food security. The pollinator-plant interaction supports the preservation of plant and animal populations and it also improves the yield in pollination dependent crops. Having knowledge about the plant-pollinator interaction is necessary for development of pesticide risk assessment of pollinators and conservation of endangering species. Results: Traditional methods to discover the relatedness of insects and plants are based on tracing the visiting pollinators by field observations as well as palynology. These methods are time-consuming and needs expert taxonomists to identify different groups of pollinators such as insects or identify flowering plants through palynology. With pace of technology, using molecular methods become popular in identification and classification of organisms. DNA metabarcoding, which is the combination of DNA barcoding and high throughput sequencing, can be applied as an alternative method in identification of mixed origin environmental samples such as pollen loads attached to the body of insects and has been used in DNA-based discovery of plant-pollinator relationship. Conclusions: DNA metabarcoding is practical for plant-pollinator studies, however, lack of reference sequence in online databases, taxonomic resolution, universality of primers are the most crucial limitations. Using multiple molecular markers is preferable due to the limitations of developed universal primers, which improves taxa richness and taxonomic resolution of the studied community.

Characterization of Algal Community of Yongdam Reservoir and Identification of Ecological Factors Inducing the Changes in Community Composition (용담호 조류군집의 시공간적 분포와 조류발생 요인분석)

  • Kim, Hyun-su;Jeong, Il-hwan
    • Journal of Soil and Groundwater Environment
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    • v.20 no.7
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    • pp.121-134
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    • 2015
  • Spatial and temporal changes in algal population in Yongdam reservoir and ecological factors that induced the changes in the size and composition of algal population were investigated by monthly sampling at ten locations in the reservoir. Nutritional state of the reservoir was identified to be phosphorus-limited with nitrogen to phosphorus (N : P) ratio much greater than 17 in most samples. Algal population was dominated by three taxonomic groups, diatoms, chlorophytes and cyanobacteria. Although explosive algal growth was not observed in the summer, algal population showed transition with time of the dominant algal type from diatoms in the winter to cyanobacteria in the summer. Chlorophyta was not the dominant group in the reservoir although they maintained relatively stable number of cells in the reservoir and showed increase in population from March to May. The application of statistical methods revealed that the factors inducing changes in cell number of each group were water temperature for diatoms and cyanobacteria and phosphorus concentration for chlorophyte. Fluctuation of cyanobacterial population was mainly observed near the inlet of tributaries while diatoms showed higher variation inside the reservoir.

Survey of Alcoholic and Non-alcoholic Beverage Preference in College Students of the Chonnam Area (일부 전남지역 대학생들의 알코올 음료 섭취 실태와 음료의 기호도 조사)

  • 정복미;오은실;최성미;차연수
    • Korean Journal of Community Nutrition
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    • v.6 no.3
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    • pp.290-296
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    • 2001
  • This study investigated the intake of alcoholic and non-alcoholic beverages in college students. Five hundred and eighty seven students age 19-30 (432 male and 155 female) responded to the beverage consumption survey. Of the students 19.9% were freshman, 42.2% sophomore, 23% junior, and 15% seniors. Results are summarized as follows : 1) Beer and soju were the most commonly consumed alcoholic beverages by the college students. The amount of beverage normally consumed was 3 cans of beer or 1 bottle of soju. 2) There was no age related change in amount of alcoholic beverage consumed, but preference for liquor rather than beer increased with age. 3) Foods most commonly consumed prior to drinking were cooked rice and milk. 4) Following the drinking of alcoholic beverages the most commonly consumed food or beverage was cold water for both males and females. The next most commonly foods were cooked rice, instant noodles, and cola for males ; and cooked rice, milk, and fruit for females. 5) Cola and pear juice were the preferred non-alcoholic beverages for college students. Also popular among students were date juice for males and orange juice for females. Milk and non-cola carbonated beverages were not commonly consumed. This study provides information for the identification of possible alcoholic beverage related public health risks among college students.

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Opportunities and Challenges in Nutrigenomics and Health Promotion

  • Milner John A.
    • Proceedings of the Korean Society of Food Science and Nutrition Conference
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    • 2004.11a
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    • pp.17-23
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    • 2004
  • Not all individuals respond identically, or at times in the same direction, to dietary interventions. These inconsistencies likely arise because of diet and genomic interactions (nutrigenomics effects). A host of factors may influence the response to bioactive food components including specific polymorphisms (nutrigenetic effect), DNA methylation patterns and other epigenomic factors (nutritional epigenomic effects), capacity to induce anuo. suppress specific mRNA expression and patterns (nutritional transcriptomics), the occurrence and activity of proteins (proteomic effects), and/or the dose and temporal changes in cellular small molecular weight compounds will not only provide clues about specificity in response to food components, but assist in the identification of surrogate tissues and biomarkers that can predict a response. While this 'discovery' phase is critical for defining mechanisms and targets, and thus those who will benefit most from intervention, its true usefulness depends on moving this understanding into 'development' (interventions for better prevention, detection, diagnosis, and treatment) and a 'delivery' phase where information is provided to those most in need. It is incumbent on those involved with food and nutrition to embrace the 'omics' that relate to nutrition when considering not only the nutritional value of foods and their food components, but also when addressing acceptability and safety. The future of 'Nutrigenomics and Health Promotion' depends on the ability of the scientific community to identity appropriate biomarkers and susceptibility variants, effective communications about the merits of such undertakings with the health care community and with consumers, and doing all of this within a responsible bioethical framework.

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Review on the Trends of Social Sciences Research in the International Agricultural Research Institute - Focused on the International! Rice Research Institute (IRRI) - (국제농업연구기관의 사회과학연구 동향 고찰 -국제미작연구소를 중심으로-)

  • 윤순덕
    • The Korean Journal of Community Living Science
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    • v.15 no.3
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    • pp.45-56
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    • 2004
  • The purpose of this paper is to review research evolution and achievements, major activities, research manpower, and difficulties for social scientists, using IRRI's internal web site and Social Sciences Division's research literatures. The major findings are as follows: 1) Social research started with the establishment of Agricultural Economics Division (AED) in 1966, and it was developed into the Social Sciences Division (SSD) in 1990; 2) Their research has been geared towards developing technological and policy interventions that improve food security and raise the well-being of rice farmers through sustainable increase in the productivity of a rice-based production system; 3) Major activities of social sciences research for about four decades can be classified into the identification of technology needs, technology impact assessment, policy analysis, research prioritization, and capacity building for social science research in NARS (National Agricultural Research Systems). They have become increasingly important in alleviating the poverty of the rice growing and consuming population; 4) Social researchers at IRRI include economists, anthropologists, gender specialists, geographic information specialists, and rural sociologists; 5) Finally, this paper discusses the difficulties faced by social researchers.

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Numerical Analysis of Bacterial Community in Cheonsu bay (천수만 해역 세균 군집의 수리학적 분석)

  • 정현미;김명운;이건형;김상종
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.265-271
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    • 1989
  • Bacteria isolated from Cheonsu Bay at 4 seasons were analyzed by numerical taxonomic method. Results of 48 morphological, physiological and biochemical tests showed different adaptability of bacteria to temperature in consequence with sampling season and isolated bacteria were able to survive at various environmental conditions, Identification results revealed that Enterobacteriaceae, Aeromonas, Pseudomonas and Vibrio were dominant genera in geterotrophic bacterial community. For each season, Aeromonas was most dominant in spring and autumn, Pseudomonas and Enterobacteriaceae in summer and winter, respectively. Cluster ananlysis was performed and all vacteria were clustered into 29 phenetic groups. Seasonal characteristics were distict in each group. Different physiological characteristics and species compositions for each season contribute to the stability and diversity of environmental ecosystem.

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Analyzing Creativity of Early Childhood Preservice Teacher based on Gender Roles Identity (예비유아교사의 성역할 정체감에 따른 창의성의 차이)

  • Youn, Jeong-Jin;Seo, Hyun-Ah
    • The Korean Journal of Community Living Science
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    • v.21 no.2
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    • pp.191-200
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    • 2010
  • The purpose of this study was to research the differences between gender roles and creativity. This study was done based on 178 pre-service teachers who were from the Department of Early Childhood Education in Universities around the Busan area. The researchers have collected statistical data by questioning pre-service teachers about creative thinking tests, creative personality tests, and gender role identification awareness tests. The data was interpreted by the Paerson's Simple Product-moment Correlation Coefficient method, the one-way ANOVA method, and the $Sch\acute{e}ffe$ Post-hoc comparison method. According to this study, the group perceived of high androgyny type group showed the highest level in important factors of creative thinking, such as fluency, elaborateness, ness, and openness. This result meant that the more a pre-service teacher was aware of the identity of gender roles, the more she or he thought creatively. Additionally, the acceptance of authority, an element of the creative personality factor, showed the highest level in a high feminity type group. On the other hand, self confidence, inquisitiveness, and disciplined imagination showed the highest level in a group which perceived the identity of androgyny type roles.

Participation in Decision-making and Expertise of Staff Nurses (일부종합병원 일반간호사의 의사결정 참여와 전문성)

  • Cho, Mee-Kyung;Jeong, Hyun-Sook
    • Research in Community and Public Health Nursing
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    • v.10 no.2
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    • pp.537-548
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    • 1999
  • The purpose of this study was to analyze the relationship of the participation in decisionmaking and expertise of staff nurses. The population for this study was the registered nurses(N=342) working in Chungnam and Chungbuk. The data were collected from April 26 to May 26, 1999. The survey instrument was Participation in Decision Activities Quesionnaire and Expertise scale developed by Anthony(1995), and Job Expertise scale of Van de Ven and Ferry. The results were as follows: 1) There was a significant difference for identification and design, and selection among the process of participation in decision making. 2) There was a significant difference between the participation in caregiving decisions and condition of work decisions. 3) (1) There was a significant difference among caregiving decisions between the expertise indicators and the variables such as education level. and the experience to told who is an expert. (2) There was a significant difference among condition of work decisions between the expertise indicators and the variables such as career, the spent keeping current per week, and self-rating of expertise.

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