• Journal of Korean Society of Forest Science
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• v.89 no.3
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• pp.389-396
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• 2000

To determine whether the Tricholoma matsutake (pine mushroom, Songyi) is symbiotic or parasitic to Pinus densiflora, structural change in their natural ectomycorrhizas were examined. The mycorrhizal samples were collected at three progressional points in the natural hypogeous colony(shiro) : colony front edge, near the fruiting point and 20cm back. The fine roots in the colonies were typical ectomycorrhizas with fungal mantle and Hartig net. However, the T. matsutake mycorrhizas had unique characteristics compared to other types of ectomycorrhizas. That is, spatially the fungal mantle and Hartig net of the T. matsutake mycorrhizas continued to develop along the growing tip, while temporally those structures declined to shrink changing to black brown in the older part of the roots behind the actively growing tip portion. However, there was no mark that the fungal hyphae penetrated into either the cortical cells, endodermal cell layers or stele. The apical tips of the blackened roots remained alive to form new mycorrhizas with other fungi later. Therefore, we conclude that the mycorrhiza of T. matsutake+P. densiflora is rather a dynamic symbiosis that changes its position spatiotemporally as the root grows than either a simple parasitism or symbiosis.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.12
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• pp.4773-4780
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• 2014

Background: To investigate the effects of small interference RNA (siRNA) targeting BCR/ABL mRNA on proliferation and apoptosis in the K562 human chronic myeloid leukemia (CML) cell line and to provide a theoretical rationale and experimental evidence for its potential clinical application for anti-CML treatment. Materials and Methods: The gene sequence for BCR/ABL mRNA was found from the GeneBank. The target gene site on the BCR/ABL mRNA were selected according to Max-Planck-Institute (MPI) and rational siRNA design rules, the secondary structure of the candidate targeted mRNA was predicted, the relevant thermodynamic parameters were analyzed, and the targeted gene sequences were compared with BLAST to eliminate any sequences with significant homology. Inhibition of proliferation was evaluated by MTT assay and colony-formation inhibiting test. Apoptosis was determined by flow cytometry (FCM) and the morphology of apoptotic cells was identified by Giemsa-Wright staining. Western blotting was used to analyze the expression of BCR/ABL fusion protein in K562 cells after siRNA treatment. Results: The mRNA local secondary structure calculated by RNA structure software, and the optimal design of specific siRNA were contributed by bioinformatics rules. Five sequences of BCR/ABL siRNAs were designed and synthesized in vitro. Three sequences, siRNA1384, siRNA1276 and siRNA1786, which showed the most effective inhibition of K562 cell growth, were identified among the five candidate siRNAs, with a cell proliferative inhibitory rate nearly 50% after exposure to 12.5nmol/L~50nmol/L siRNA1384 for 24,48 and 72 hours. The 50% inhibitory concentrations ($IC_{50}$) of siRNA1384, siRNA1276 and siRNA1786 for 24hours were 46.6 nmol/L, 59.3 nmol/L and 62.6 nmol/L, respectively, and 65.668 nmol/L, 76.6 nmol/L, 74.4 nmol/L for 72 hours. The colony-formation inhibiting test also indicated that, compared with control, cell growth of siRNA treated group was inhibited. FCM results showed that the rate of cell apoptosis increased 24 hours after transfecting siRNA. The results of annexinV/PI staining indicated that the rate of apoptosis imcreased (1.53%, 15.3%, 64.5%, 57.5% and 21.5%) following treamtne with siRNAs (siRNA34, siRNA372, siRNA1384, siRNA1276 and siRNA1786). Morphological analysis showed td typical morphologic changes of apoptosis such as shrunken, fragmentation nucleus as well as "apoptotic bodies" after K562 cell exposure to siRNA. Western blot analysis showed that BCR/ABL protein was reduced sharply after a single dose of 50nmol/L siRNA transfection. Conclusions: Proliferation of K562 cells was remarkbly inhibited by siRNAs (siRNA1384, siRNA1276 and siRNA1786) in a concentration-dependent manner in vitro, with effective induction of apoptosis at a concentration of 50 nmol/L. One anti-leukemia mechanism in K562 cells appeared that BCR/ABL targeted protein was highly down-regulated. The siRNAs (siRNA1384, siRNA1276 and siRNA1786) may prove valuable in the treatment of CML.

• Journal of the Korean association of regional geographers
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• v.6 no.3
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• pp.1-36
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• 2000

Periodic markets of the later Choson dynasty had undergone fundamental changes during the late Chosun dynasty and Japanese colony period. This paper aims to analyse the spatial distributions and changes of the periodic markets in the middle stream of Han-River in this era in the use of the survey of documentary records and fieldwork. Before the early 1910s, long distance transportation was made by riverboats, short distance transportation was done by porters and pack animals. Because goods such as rice, soybean and salt were very heavy and needed long distance transport, they were mainly transported by riverboats. Accordingly, riverports on the shore of river played important roles in exporting and importing goods as nodes of long distance transportation. The opening of railroad Seoul-Busan, the construction of new roads(Sinjakno) of 1910s and the use of oxcarts produced striking changes in the spatial distribution and hierarchy structure of periodic markets. These changes also had influence on the outflow and inflow of goods in the middle stream of Han-River. In the parr of outflow of goods, it seems that the line linking Icheon city-Yongin city-Anseong city played a role in making the boundaries of the marketing areas between goods trams ported by the rail road Seoul-Busan and goods done by Han River river boats. Anseong, Osan and Suwon periodic markets located around railroad stations occupied the higher positions than those of other regions in the hierarchial structure of periodic markets. Their marketing areas could nearly overwhelm those of riverports located in the middle stream region of Han River and extend to the middle stream region of Han River as a result of decrease of transport cost through using of oxcarts and railroads. the opening of railroad Suwon-Yeoju(Suryoson) and railroad Cheonan-Janghawon(Cheonjangson) brought about changes of the structure of long distance trade again. In a part of outflow of goods, it seems that as a result of the new opening of their railroads periodic markets around railroads seized a large portion of the marketing area of the southern part of Yoju and Ichon area and therefore made extreme change in rivershipping of Han River. In the inflow part of goods, goods transported by riverboats from the downstream of Han River before the opening of railroad were imported directly from railroad stations. Accordingly, rivershipping and riverports declined. And because goods were imported by way of great periodic markets and supplied to small periodic markets and consumers before the opening of railroad, but supplied from railroad stations to small periodic markets and consumers after the opening of railroad. The volume of turnover of such great periodic markets as Anseong, Osan and Suwon periodic markets therefore declined. On the other hand, because Yangpyong area had not been yet included within the sphere of influence of railroad until 1930s, it heavily depended on rivershipping of Han River as before. But the opening of railroad Seoul-Wonju(jungangson) brought about decline of rivershipping in Yangpyong and Wonju area.

• Korean Journal of Environment and Ecology
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• v.32 no.1
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• pp.105-117
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• 2018

This study conducted health assessment and multivariate vegetation analysis using the riparian vegetation index in 30 sites of the Geumgang mainstream and Mihocheon to obtain practical data on the river management of the Geumgang. The result showed that the number of plant communities was 54. The flora was 75 families, 185 genera, 243 species, 2 subspecies, 21 varieties, 2 varieties, and 268 taxa. The riparian vegetation index was 38.3 (3.3; G-D1 ~ 66.7; G-U2, G-U4, and G-M3), and the health of the rivers in this area was evaluated as normal (grade C). The health of rivers was the highest in the upper stream of Geumgang mainstream and lowest in the downstream of Geumgang mainstream. The relationship between riparian vegetation index and chlorophyll-a content was low. The riparian vegetation was divided into five groups of Digitaria ciliaris colony group, Salix gracilistyla colony group, Erigeron annuus colony group, the group dominated by Humulus japonicus, Salix koreensis, Miscanthus sacchariflorus, and Phragmites japonica colonies, and the group dominated by Conyza canadensis and Echinochloa crusgalli var. echinata colonies. They had the similar health conditions. The CCA analysis showed that the environmental factors affecting the distribution of vegetation were physical factors such as vegetation area, artificial structure area, waterway area, branch width, channel width, and bank height and the biological factors such as the number of species. As such, it is necessary to maintain the health condition through continuous monitoring where the health condition is high and to apply active measures such as ecological restoration where the health condition is low.

• 보존과학연구
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• s.22
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• pp.41-52
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• 2001

The wooden building is mainly damaged by the termite which have an effect of the structure by making emptied inner part of wood. One class, Japanese termite, inhabits throughout Korea and is often detected. So the deterioration by termite attacking the wooden building needs to be controlled. Termites are over 2,800 species in the world, usually they inhabit in the tropical or subtropical region and don’t over winter in dormant condition. So their activity and distribution are chiefly restricted by temperature and humidity. The termite inhabiting in Korea is Reticulitermes speratus kyushuensis Morimoto, which known to have an optimal temperature range at $12~30^{\circ}C$ and minimum temperature at $6^{\circ}C$ for activation. These temperatures correspond to the mean temperature($5.6^{\circ}C$~$25.8^{\circ}C$) from March to November and the activity time of termitein Seoul. In addition, as a result of environmental pollution by industrial development, the climate of Korea is getting warm. The increase of mean temperature in Korea has been $1.1^{\circ}C$ for the past seventy five years, so it is expected that the damage of wooden building by termite will increase gradually. Therefore, in order to protect wooden building from damages by termite, it needs not only development of new pest control methods, but also studies on the control of environmental factors having an effect on the activity and growth of termite. For the conservation of the large cultural properties such as the wooden building in the open air, it would be effective to use the methods of fumigation, insecticidal and antiseptic chemical treatment of wood materials, soil termiticideinjection treatment, and termite colony elimination system.

• Journal of the Korean Society of Food Culture
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• v.26 no.6
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• pp.539-553
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• 2011

This study was intended to examine the continuance and transformation of food culture during the enlightenment and Japanese ruling era by analyzing the novel of "Toji". In the novel "Toji", the chaotic political and economic situation is reflected, along with the peoples' hard lives in the latter era of the Choson Dynasty. After the full-fledged invasion of China by the Japanese, the shift to a wartime posture was accompanied by an increased need for food. This led to a rationing and delivery system for rice in the late 1930s. While it was hard for people to obtain even brewer's grains and bean-curd dregs, food distribution officers were well off. Another distinctive feature of the food culture during the enlightenment and Japanese ruling era was that foreign food and recipes were introduced naturally to Korea through the influx of various foreigners. The industry of Choson was held by Japanese monopolistic capital, as a result, Choson had equal to the role as a spending site and was only gradually left destitute. In the Japanese ruling era, there were new type of business including such as patisserie of the types of civilization in the town, and those tempted Korean people. However, the Japanese and pro-Japan collaborators dominated commercial business. Being urbanization through the modernization, it was became patronized fast food in the populous downtown, and the change of industry structure and life style greatly influenced into our food culture. Acceptance the convenient Japanese style fast food such as Udong, pickled radish made was actively accepted with a longing for the advancement civilization. After the enlightenment, many Japanese exchange students went to Tokyo to get advanced civilization and provided urban mood according to their consumption of bread, coffee, Western food, which were considered a part of the elite culture.

• Mycobiology
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• v.46 no.2
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• pp.92-100
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• 2018

The filamentous Ascomycota Colletotrichum gloeosporioides sensu lato is a fungus that has been reported worldwide as a causal agent of anthracnose disease in avocado and other crops. In Mexico, this species affects fruits from an early stage of development in the orchard until the post-harvest stage. Although fungicides are continuously applied to control Colletotrichum species, pericarp cankers and soft rot mesocarp in fruits are still frequently observed. Considering the lack of a precise description of the causative agent, the aim of the current study was to determine the pathogens involved in this symptomatology. Twenty-four isolates were consistently obtained from the pericarp of avocado fruits cv. "Hass" collected in the central avocado-producing area of Mexico. Morphological features such as colony growth, conidia size, and mycelial appressorium were assessed. Bayesian multilocus phylogenetic analyses were performed using amplified sequences of the internal transcribed spacer region of the nuclear ribosomal DNA; actin, chitin synthase, glyceraldehyde-3-phosphate dehydrogenase partial genes; and APn2-Mat1-2 intergenic spacer and mating type Mat1-2 partial gene from the nine selected isolates. In addition, fruits were inoculated with a conidial suspension and reproducible symptoms confirmed the presence of Colletotrichum fructicola in this area. This pathogenic species can now be added to those previously reported in the country, such as C. acutatum, C. boninense, C. godetiae, C. gloeosporioides, and C. karstii. Disease management programs to reduce the incidence of anthracnose should include C. fructicola to determine its response to fungicides that are routinely applied, considering that the appearance of new species is affecting the commercial quality of the fruits and shifting the original population structure.

• International Journal of Industrial Entomology and Biomaterials
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• v.31 no.1
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• pp.14-19
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• 2015

In a previous report, we identified several candidate antimicrobial peptides through de novo RNA sequencing of the centipede Scolopendra subspinipes mutilans. Here, we identify and characterize one of these peptides, Scolopendrasin I. We identified the centipede antimicrobial peptide Cecropin from the centipede transcriptome using an SVM algorithm, and subsequently analyzed the amino acid sequence for predicted secondary structure using a GOR algorithm. We identified an alpha helical region of Cecropin and named it Scolopendrasin I. We then assessed antimicrobial and hemolytic activity of Scolopendrasin I. Scolopendrasin I showed antimicrobial activity against various microbes, including antibiotic-resistant Gram-negative bacteria, in a radial diffusion assay. Scolopendrasin I had potent antibacterial activity against acne-associated microbes in a colony count assay and showed no hemolytic activity in a hemolysis assay. In addition, we confirmed that Scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, two components of bacterial cell membranes. In conclusion, the results presented here provide evidence that this is an efficient strategy for antimicrobial peptide candidate identification and that Scolopendrasin I has potential for successful antibiotic development.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.269-274
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• 2018

In the middle of May 2018, typical red thread disease symptoms were observed on Kentucky bluegrass (Poa pratensis L.) on a golf course, which locates at Yangsan, Gyeongnam province in Korea. Irregular-shaped patched symptoms were observed in fairway of golf course. The foliar symptom was dried out and faded to straw color and tip of the grass leaves were tangled like thread. Early morning, infected and tangled leaves were covered with the pinkish gelatinous antler-like structure (sclerotinia) as a typical red thread disease symptom. Causal fungal pathogens were isolated from the symptom in Kentucky bluegrass. The fungal culture characteristic on potato dextrose agar color of colony was pale pink and conjugated hyphae, sclerotium of irregular shape was pale pink and 3~5mm diameter in size. The pathogen was identified as Laetisaria fuciformis based on morphological and culture characteristics as well as molecular characteristics. Pathogenicity test was verified on the Kentucky bluegrass by Koch's postulates. This is the first report of red thread disease occurrence in Kentucky bluegrass by L. fuciformis in Korea.

• Journal of the Korea Fashion and Costume Design Association
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• v.6 no.3
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• pp.153-167
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• 2004

Korea had suffered the colony of Japanese imperialism and the Korean War from the invasion of the external circumstances. Such special situation made new influences on the clothing habits. The types of Korean women clothing could escape from the traditional style that had been maintained for thousands of years. The foreign clothes for Korean women had undergone various changes from the Liberation in 1945. The jackets that had been introduced to Korea at the Enlightenment period had become popular outfits for men. The jackets made big influences on the changes such as the social advances for women from the times background of the Liberation. The jackets possess very important meaning for the changes in the diffusion of feminism and fashion. Therefore, this research tries to investigate the forms, silhouettes, changes and structures of the jackets such as the locations of the collar, the sleeve, and the waste line. The process of the changes in the style of women jackets is also analyzed from the silhouette and the structure method. The development and changes will be understood of Korean women's jackets. The Korean women's jackets should also be examined further with the history of foreign clothing from now on. The fashion to be introduced from important society cultural phenomena and the Western Europe has been reflected into the changes in the style of Korean female jackets. Also, dramatic changes in the style of clothing indicate that the society had experienced a great deal of changes. It is verified that foreign clothes have been interacted with the stream of times worldwide. After the Liberation, the female jackets become a uniform style which just followed the Western European style. However, a season trend has been announced from the SFAA since 1990. Since then trendy jackets have been detailed more with this chance.