Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
권호 표지
DOI QR코드

DOI QR Code

First Report of Red Thread of Kentucky Bluegrass (Poa pratensis L.) Caused by Laetisaria fuciformis on Golf Course in Korea

국내 골프코스의 켄터키블루그래스에 Laetisaria fuciformis에 의한 붉은뿔마름병(Red Thread) 발생보고

  • Received : 2018.08.14
  • Accepted : 2018.09.11
  • Published : 2018.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

In the middle of May 2018, typical red thread disease symptoms were observed on Kentucky bluegrass (Poa pratensis L.) on a golf course, which locates at Yangsan, Gyeongnam province in Korea. Irregular-shaped patched symptoms were observed in fairway of golf course. The foliar symptom was dried out and faded to straw color and tip of the grass leaves were tangled like thread. Early morning, infected and tangled leaves were covered with the pinkish gelatinous antler-like structure (sclerotinia) as a typical red thread disease symptom. Causal fungal pathogens were isolated from the symptom in Kentucky bluegrass. The fungal culture characteristic on potato dextrose agar color of colony was pale pink and conjugated hyphae, sclerotium of irregular shape was pale pink and 3~5mm diameter in size. The pathogen was identified as Laetisaria fuciformis based on morphological and culture characteristics as well as molecular characteristics. Pathogenicity test was verified on the Kentucky bluegrass by Koch's postulates. This is the first report of red thread disease occurrence in Kentucky bluegrass by L. fuciformis in Korea.

2017년 4월 중순 경남 양산에 소재한 골프장에서 Laetisariafuciformis에 의한 redthread 병징이 켄터키블루그래스에 발생하였다. 골프코스의 페어웨이에 불규칙한 모양의 병반 형태로 발생하였다. 감염된 잎은 마름증상을 보이면서 점차 볏짚색으로 변했으며, 잎의 끝부분은 실모양으로 헝클어져있었다. 이른 아침에는 분홍빛을 띤 젤라틴 형태로된 사슴뿔 모양의 균핵을 형성하였다. 배양적 특징으로 균총은 옅은 분홍색이었으며 균사다발이 형성되었고 3-5mm 크기의 연한 분홍색 균핵을 형성하였다. 병원균은 형태, 배양적특징 및 분자생물학적 특징을 토대로 L. fuciformis로 동정되었다. 코흐의 원칙에 따라 병원성 검정을 실시하여 켄터키블루그래스에 병원성이 있음을 확인하였다. 이는 한국에서 L. fuciformis에 의해 켄터키블루그래스에 발생하는 redthread에 대한 최초의 보고이다.

Keywords

References

  1. Graham, J., Marshall, B. and Squire, G. 2003. Genetic differentiation over a spatial environmental gradient in wild Rubus idaeus populations. New Phytol. 157:667-675. https://doi.org/10.1046/j.1469-8137.2003.00693.x
  2. Johnson, B. J. and Bowyer, T. H. 1982. Management of herbicide and fertility levels on weeds and Kentucky bluegrass. turf. Agron. Journal. 74:845-850.
  3. Kaplan, J.D. and Jackson, N. 1983. Red thread and pink patch disease of turf grasses. Plant Dis. 67:159-162. https://doi.org/10.1094/PD-67-159
  4. Kumar, S., Tamura, K. and Tamura, K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7):1870-1874. https://doi.org/10.1093/molbev/msw054
  5. Lee, S.K. 2012. Irrigation frequency for Kentucky Bluegrass (Poa pratensis) growth. Asian J. Turfgrass Sci. 26(2):123-128.
  6. McAlpine, D. 1906. A new hymenomycete - the so called Isaria fuciformis. Annal. Mycol. 4:541-551.
  7. Meyer, W.A. and Funk, C.R. 1989. Progress and benefts to humanity from breeding cool-season grasses for turf. pp. 31-48. In: Sleeper, D.A., Asay, K.H. and Pedersen, J.F. (Eds.) Contributions from breeding forage and turf grasses. Crop Science Society of America Special Publication, Madison, WI, USA.
  8. Porceddu, A., Albertini, E., Barcaccia, G., Falistocco, E. and Falcinelli, M. 2002. Linkage mapping in apomictic and sexual Kentucky bluegrass (Poa pratensis L.) genotypes using a two way pseudo- testcross strategy based on AFLP and SAMPL markers. Theor. Appl. Genet. 104:273-280. https://doi.org/10.1007/s001220100659
  9. Saitou, N. and Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-25.
  10. Smiley, R.W., Dernoeden, P.H. and Clarke, B.B. 2005. Compendium of turfgrass diseases, 3rd ed. APS press, MN, USA.
  11. Stalpers, J.A. and Loerakker, W.M. 1982. Laetisaria and Limonomyces species (Corticiaceae) causing pink diseases in turf grasses. Can. J. Bot. 60(5):529-537. https://doi.org/10.1139/b82-071
  12. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599. https://doi.org/10.1093/molbev/msm092
  13. Wakefeld, E.M. 1917. Notes on British thelephoraceae. Trans. Br. Mycol. Soc. 5:474-481.
  14. Weir, B.S., Johnston, P.R. and Damm, U. 2012. Colletotrichum gloeosporioides species complex. Stud. Mycol. 73:115-180. https://doi.org/10.3114/sim0011
  15. White, T.J., Bruns, T.D., Lee, S.B. and Taylor, J.W. 1990. Amplifcation and direct sequencing of fungal ribosomal RNA genes for phylogenetics pp. 315-322. In: Innis, M.A., Gelfand,D.H. and Sninsky, J.J, (Eds.). PCR protocols: a guide to methods and applications. N.Y: Academic Press, NY, USA.