• Journal of the Korea Society of Computer and Information
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• v.29 no.10
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• pp.137-148
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• 2024

The European honeybee (Apis mellifera) is an important pollinator threatened by colony collapse disorder (CCD), primarily due to infestation by the Varroa mite (Varroa destructor). Traditional detection methods are invasive and time-consuming, often causing additional stress to colonies. We propose a two-stage framework using the You Only Look Once version 8 (YOLOv8) model for non-destructive and rapid detection of Varroa mite infestation. The framework uses comb light images from inside the hives. In the first stage, a YOLOv8-n model detects bees and extracts individual bee images. In the second stage, a YOLOv8-cls model classifies the infestation status of each bee. Our object detection model achieved a mAP@0.5 of 0.701, and the classification model achieved an average accuracy of 91%. These results demonstrate the effectiveness of the framework as a non-destructive method for Varroa mite detection. Based on this research, we expect to provide beekeepers with an efficient tool for early detection and management of Varroa mite infestations, potentially reducing the incidence of CCD and supporting the sustainability of apiculture.

• Mass Spectrometry Letters
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• v.8 no.3
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• pp.65-68
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• 2017

Thiamethoxam is one of the main suspect in honeybee colony collapse disorder (CCD). Due to this reason, thiamethoxam including imidacloprid and clothianidin has been banned for two years in some Europe countries. The CCD phenomenon has also been reported in Korea. Regarding this issue and needs, a new project has started to develop the method for the quatitation of thiamethoxam using isotope dilution mass spectrometry (IDMS). In the process of optimization for the IDMS method with thiamethoxam and $thiamethoxam-d_3$, we observed that the fragment peaks did not correspond to the fragmentation pathway as published elsewhere. Here, we proposed a candidate fragmentation pathway. To validate the proposed fragmentation pathway, another isotope analogue, $thiamethoxam-d_4$, was introduced and the MS/MS spectra of both isotope analogues were compared. In addition, the MS/MS/MS spectra of thiamethoxam were inspected for more evidence of the candidate pathway. Those spectra indicated that the proposed fragmentation pathway could be used to assign the fragment peaks of thiamethoxam.

• Journal of Apiculture
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• v.34 no.1
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• pp.47-55
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• 2019

Honey bees are a vital part of the food chain as the most important pollinators for a broad palette of crops and wild plants. The climate change and colony collapse disorder (CCD) phenomenon make it challenging to develop ICT solutions to predict changes in beehive and alert about potential threats. In this paper, we report the test results of the bee-counting system which stands out against the previous analogues due to its comprehensive components including an improved dual infrared sensor to detect honey bees entering and leaving the hive, environmental sensors that measure ambient and interior, a wireless network with the bluetooth low energy (BLE) to transmit the sensing data in real time to the gateway, and a cloud which accumulate and analyze data. To assess the system accuracy, 3 persons manually counted the outgoing and incoming honey bees using the video record of 360-minute length. The difference between automatic and manual measurements for outgoing and incoming scores were 3.98% and 4.43% respectively. These differences are relatively lower than previous analogues, which inspires a vision that the tested system is a good candidate to use in precise apicultural industry, scientific research and education.