• 인간식물환경학회지
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• 제24권1호
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• pp.53-62
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• 2021

Background and objective: Dermatitis is a chronic disease accompanied by such symptoms as itching and dry skin. The environment and diet can aggravate dermatitis, so attention to skin care is essential. Colocasia esculenta is used in various manners and for different purposes, including with regard to inflammation, aging, and the digestive system. The anti-inflammatory effect of Colocasia esculenta water extract was confirmed using RAW 264.7 macrophages with regard to male ICR mice. Methods: In the case of the ICR mice, 5% 12-O-Tetradecanoylphorbol-13-acetate (TPA) was used to cause inflammation for 7 days, and 100 μL of Colocasia esculenta water extract and panthenol were administered orally for 10 days. In addition, RT-PCR, NO, ELISA was conducted. Results: As a result of reverse transcription polymerase chain reaction (RT-PCR) using RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS), it was found that Colocasia esculenta water extract reduced the expression of inflammatory cytokines. As a result of hematoxylin and eosin (H&E) staining using mouse ear tissue, Colocasia esculenta water extract reduced ear thickness and showed an effect of suppressing ear edema. In addition, compared to the TPA-treated group, the Colocasia esculenta extract-treated group had reduced nitric oxide (NO) production by 18.23 μM and IL-13 production decreased by 136.55 pg/ml. Conclusion: Colocasia esculenta water extract has been shown to be effective in lowering inflammatory cytokine production. These results suggest that Colocasia esculenta water extracts can be used as natural products to treat dermatitis.

• Plant Resources
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• 제2권2호
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• pp.65-68
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• 1999

This study was conducted to obtain the basic breeding information of Colocasia esculenta Schott. Effect of supplemental plant growth regulators and genotype difference were investigated on dormant bud tissue for proliferation. The plant regeneration ratio, plant height and root length were the best upon mixed treatment of 0.8mg/L IAA and 2.0mg/L zeatin. Both leaf weight and root weight were heavy upon culture in a dark condition. The leaf and root weights were heaviest in 6Pie sucrose concentrations. In several collected area the heaviest one was Binnangxin and then in the order of Suwon, Wanju and Puan. Genotype differences of tuber diameter and tuber weight were found in Suwon. Tuber weight was found in the order of Suwon (862mg) >Wanju(723mg) >Puan(649mg) >Binnangxin (424mg).

• Bulletin of the Korean Chemical Society
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• 제31권7호
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• pp.2051-2053
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• 2010

• The Plant Pathology Journal
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• 제20권2호
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• pp.135-141
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• 2004

A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.

• 한국자원식물학회지
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• 제15권3호
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• pp.293-297
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• 2002

약용과 식용으로 이용이 가능한 구황작물인 토란을 활성탄(Activate Charcoal)을 시용하여 재배할 경우 생산성을 높일 수 있을 것으로 기대되어 본시험을 수행한 결과는 다음과 같다. 활성탄시용에 따른 토란의 생장은 활성탄처리가 무처리에 비하여 엽장이 크고, 엽병속수가 많아 생육이 양호하였으며, 특히 활성탄 10%∼20％가 첨가되었을 때 효과가 인정되었다. 또한 지하부의 생장에서도 활성탄10%∼20%처리가 주당 괴경수가 많고 괴경중이 무거워 양호한 경향이었다. 한편 괴경(槐莖)의 수량과 부산물인 엽병(葉柄)의 수량은 활성탄 10%∼20%처리가 생육이 양호하여 증수되었다. 이상과 같은 결과로 보아 토란을 재배할 경우 활성탄의 효과가 인정되어 앞으로 작물의 종류에 따른 농도별 시험이 계속적으로 이루어 져야 할 것으로 사료된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2001년도 추계정기 학술발표회 초록집
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• pp.64-64
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• 2001

• 한국토양비료학회지
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• 제49권4호
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• pp.388-392
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• 2016

The objectives of fertilizer recommendation are to prevent the application of excessive fertilization and to produce target yields. Also, optimal fertilization is important because crop quality can be influenced by fertilization. In this study, yields and fertilizer use efficiency of Taro (Colocasia esculenta Schott) were evaluated in different level of NPK fertilization. N, P and K fertilizer application rates were 5 levels (0, 50, 100, 150, 200%) by practical fertilization ($N-P_2O_5-K_2O=180-100-150kg\;ha^{-1}$), respectively. In the N treatment, the yields of Taro tuber were about $33Mg\;ha^{-1}$ from 90 to $360kg\;ha^{-1}$ N fertilization. However, the ratio of tuber to total biomass decreased with increasing N fertilization rate. In the P and K treatments, yields of Taro tuber were the highest at $150kg\;ha^{-1}$ fertilization. Fertilizer use efficiency was decreased by increase of N and K fertilization. Crude protein of Taro tuber was the highest at practical fertilization. Sucrose content of tuber was influenced by phosphate application.

• 환경생물
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• 제29권2호
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• pp.92-97
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• 2011

수경재배와 토양재배를 통해 키운 토란이 생리 형태학적으로 어떤 차이가 나는지 조사하였다. Hoa.+IAA를 처리한 수경재배는 질석, Hoa., 토양, 물에서 키운 토란보다 약 9%, 32%, 38% 및 60% 생장이 각각 촉진되었다. 물에서 재배한 토란은 5~6주 생장한 후의 $F_v/F_m$값이 0.54와 0.55를 나타내어 $F_v/F_m$값이 0.8 이상을 보인 Hoa.+IAA, 질석과 토양에 비해 약 32.5% 낮았다. Hoa.+IAA 처리구에서는 가장 낮은 확산저항이 나타났으며 5주와 6주 때는 물로 수경재배한 것에 비해 45~35% 낮았다. 이는 기공 열림이 물로 재배한 것에 비해 45~35% 크게 열렸다고 추측할 수 있다. 질석과 토양에서 자란 토란도 물로 재배한 것에 비해 39~31%와 27~35% 확산저항이 낮았다. 따라서 생장률의 크기, 광합성 활성 및 기공열림은 Hoa.+IAA, 질석, Hoagland 용액, 토양과 물 순으로 낮게 나타났다. IAA를 Hoagland 용액에 넣어 수경재배한 실험 결과에서 생장률과 형광 활성도가 가장 높았으며 확산저항 또한 가장 낮게 나타났다. 토양재배에서 점토와 상토를 포함하는 흙은 질석에서 키운 토란에 비해 뿌리 발달이 저조하였다. 화분 속의 흙은 중력에 의해 시간이 지날수록 단단해졌으나 질석은 흙과의 마찰이 비교적 적기 때문에 수염뿌리와 실뿌리가 잘 생장하였다. 화분에서 키운 토란은 종아와 뿌리가 같이 발달하였으나 종아는 하나만 관찰되었다. 반면에, 야외에서 키운 토란은 30~40개의 종아가 뚜렷하게 발달하여 작물로서의 가치가 충분하였으나 수경재배한 토란은 종아가 분해되어 사라지고 수염뿌리가 발달하였다.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.191-198
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• 2008

Germplasm characterization and evolutionary process in viable populations are important links between the conservation and utilization of plant genetic resources. Here, an investigation is made, based on molecular and biochemical techniques for assessing and exploiting the genetic variability in germplasm characterization of taro, which would be useful in plant breeding and ex situ conservation of taro plant genetic resources. Geographical differentiation and phylogenetic relationships of Indian taro, Colocasia esculenta (L.) Schott, were analyzed by random amplified polymorphic DNA (RAPD) and isozyme of seven enzyme systems with specific reference to the Muktakeshi accession, which has been to be proved resistant to taro leaf blight caused by P. colocasiae. The significant differentiations in Indian taro cultivars were clearly demonstrated by RAPD and isozyme analysis. RAPD markers showed higher values for genetic differentiation among taro cultivars and lower coefficient of variation than those obtained from isozymes. Genetic differentiation was evident in the taro accessions collected from different regions of India. It appears that when taro cultivation was introduced to a new area, only a small fraction of genetic variability in heterogeneous taro populations was transferred, possibly causing random differentiation among locally adapted taro populations. The selected primers will be useful for future genetic analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers identified in the DNA fingerprinting study will be useful for screening a segregating population, which is being generated in our laboratory aimed at developing a taro genetic linkage map.

• 한국식품과학회지
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• 제52권6호
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• pp.595-603
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• 2020

토란에 존재하는 점질다당의 새로운 이용방안을 모색하기 위하여 토란으로부터 다당을 분리하여 항보체 활성을 평가하고 구조 분석을 행하였다. 토란으로부터 분리한 조다당 CE를 이온교환수지와 Sephadex G-100 column를 이용하여 정제하였고, 그 중 수율과 활성이 양호한 CE-4a를 최종 획분으로 선정하였다. 초기 면역반응에 중요한 역할을 하는 보체계에 대한 토란 다당의 활성화 여부를 측정한 결과 양성대조군인 PSK에 준하는 강력한 항보체 활성을 보였고, 시료의 농도차이를 두어 실험한 결과 농도 의존적임을 알 수 있었다. CE-4a는 분자량 약 182.4 kDa의 다당체로 구성당 조성을 확인한 결과 Man, Gal 및 GalA를 높은 비율로 함유하고 있었다. 본 당쇄의 결합양식을 규명하기 위하여 methlyation analysis를 행한 결과 CE-4a는 terminal Galp. 3-linked-Galp, 4-linked Manp, 2,4,6-linked Manp를 포함한 총 10종의 결합으로 구성되어 있었다. 또한 CE-4a의 전체구조를 추정하기 위하여 endo-α-(1→4)-polygalacturonase, exo-α-galactosidase 및 endo-β-(1→4)-mannanase를 이용한 연속 가수분해 처리 및 해석을 행하였다. 결과를 종합하면, 토란 유래 다당 CE-4a는 (1→4)-mannan 주쇄로 존재하며 주쇄인 mannose의 C(O)6 위치에서 한가닥 또는 C(O)2, C(O)6 위치에서 동시에 두가닥의 측쇄가 연결되어 존재하고, 측쇄는 주로 galacto oligo당이 측쇄로 분지된 특징이 있음을 확인할 수 있었다.