• 한국식물병리학회지
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• 제10권2호
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• pp.105-111
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• 1994

Pathogenic fungal species associated with apple anthracnose were studied with diseased fruits collected over two major apple growing areas from 1986 to 1988. Colletotrichum acutatum Simmonds, c. gloeosporioides(Penz.) Sacc. and Glomerella cingulata(Stonem.) Spauld. & Schrenk were identified based on their mycological characteristics. C. acutatum could be easily differentiated from C. gloeosporioides by producing their fusiform conidia and developing apricot∼salmon colony colors, non-forming an ascigerous stages. Growth rate of C. acutatum were slow in plate culture. The three species of anthracnose fungi tested caused apple rot. Symptoms produced by C. gloeosporioides were larger than those of the others. C. gloeosporioides and G. cingulata produced rot lesions on apples that appeared became brown and later black, but C. acutatum produced rot lesions appeared yellowish brown and later reddish brown. G. cingulata has the major species responsible for apple anthracnose, epidemics in Korea.

• The Plant Pathology Journal
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• 제29권3호
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• pp.317-322
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• 2013

For the past two decades, the causal agent of anthracnose occurring on strawberry in Korea was considered Colletotrichum gloeosporioides. However, the recent molecular analysis has shown that the genus Colletotrichum has undergone many taxonomic changes with introduction of several new species. As a result, it revealed that C. gloeosporioides indeed consisted of more than 20 distinct species. Therefore, the Korean pathogen isolated from strawberry should be reclassified. The shape and size of the conidia of the pathogen were not distinctly different from those of C. gloeosporioides and C. fructicola, but it differed in shape of the appressoria. A combined sequence analysis of partial actin, glyceraldehydes-3-phosphate dehydrogenase genes, and the internal transcribed spacer regions showed that the strawberry isolates formed a monophyletic group with authentic strains of C. fructicola. On the basis of these results, the anthracnose fungi of the domestic strawberry in Korea were identified as C. fructicola and distinguished from C. gloeosporioides.

• Mycobiology
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• 제30권1호
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• pp.57-59
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• 2002

Anthracnose symptoms severely occurred up to 100% on leaves of May lily grown in four locations in Korea during a disease survey in 2001. The symptoms appeared as circular to irregular spots with brown to dark brown discoloration on leaves of the plant, and severely infected leaves blighted. A total of 35 isolates of Colletotrichum sp. was obtained from the spotted lesions and identified as Colletotrichum liliacearum based on the morphological and cultural characteristics. Leaf spots similar to the original anthracnose symptoms were induced on the host leaves by artificial inoculation with the isolates of the fungus. This is the first report that C. liliacearum causes anthracnose of May lily.

• 한국식물병리학회지
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• 제12권4호
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• pp.455-458
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• 1996

난 탄저병균의 배양적 특성과 탄저병의 발생 특성을 조사하였다. 자연 발병한 양란과 호접란의 잎에는 초기에 암갈색 또는 검은색의 둥글고 작은 반점이 형성되어 점차 진전되면서 검게타고 병이 진전됨에 따라 잎전체가 고사하고 심할 경우 줄기와 식물체 전체가 고사하였다. 탄저병에 감염된 양란과 호접란의 병환부로부터 분리된 Colletotrichum gloeosporioides는 상처접종에 의해서만 각각 양란과 호접란 병원성을 나타내었고, 덴파레 및 춘란에 대해서도 모두 병원성을 나타내었다. 양란은 상위엽일수록 탄저병균에 대해 감수성을 나타내었으며, 호접란이 탄저병균에 대해 감수성을 나타낸 반면에 춘란이 가장 저항성을 나타내었으며 양란과 덴파레는 중도 반응을 보였다. 탄저병균의 균사생장과 분생포자형성 적온은 각각 $25^{\circ}C$였으며, 베노밀 수화제와 비타놀 수화제에 대하여 감수성을 나타내었다.

• 농업생명과학연구
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• 제50권5호
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• pp.251-255
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• 2016

In year of 2013, anthracnose disease symptoms were observed on stem of stonecrop in a plant resource field, Southern Forest Resource Research Center, Jinju, South Korea. The disease symptoms were appeared 2-5 cm smudged irregular black spot color on the plant stems. Suspected pathogen was isolated from the lesion with surface sterilization method and identified it as Colletotrichum gloeosporioides by morphological characteristics and phylogenetic analyses. Artificially inoculated the isolated pathogen led to the similar disease symptom as originally observed in the field. The fungal pathogen was re-isolated from the inoculated lesion and it exhibited the same characteristics compared to the original isolate. This is the first report of anthracnose of stonecrop grown in open fields, caused by Colletotrichum gloeosporioides in Korea.

• 한국균학회지
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• 제51권4호
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• pp.335-347
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• 2023

Sordariomycetes is the second largest class of Ascomycota, distributed throughout various habitat including terrestrial and aquatic environments and also existing as endophytes. We isolated endophytic fungal strains in Korea, identifying them based on their morphological characteristics and molecular analyses, using eight specific DNA regions for accurate genus identification. We identified five previously unrecorded endophytic fungal species in Korea: Chaetomium subaffine, Colletotrichum jiangxiense, Colletotrichum sydowii, Diaporthe vacuae and Neurospora tetraspora. In this study, we describe the morphological characteristics and present our phylogenetic analyses of these five fungal species.

• Mycobiology
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• 제46권2호
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• pp.92-100
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• 2018

The filamentous Ascomycota Colletotrichum gloeosporioides sensu lato is a fungus that has been reported worldwide as a causal agent of anthracnose disease in avocado and other crops. In Mexico, this species affects fruits from an early stage of development in the orchard until the post-harvest stage. Although fungicides are continuously applied to control Colletotrichum species, pericarp cankers and soft rot mesocarp in fruits are still frequently observed. Considering the lack of a precise description of the causative agent, the aim of the current study was to determine the pathogens involved in this symptomatology. Twenty-four isolates were consistently obtained from the pericarp of avocado fruits cv. "Hass" collected in the central avocado-producing area of Mexico. Morphological features such as colony growth, conidia size, and mycelial appressorium were assessed. Bayesian multilocus phylogenetic analyses were performed using amplified sequences of the internal transcribed spacer region of the nuclear ribosomal DNA; actin, chitin synthase, glyceraldehyde-3-phosphate dehydrogenase partial genes; and APn2-Mat1-2 intergenic spacer and mating type Mat1-2 partial gene from the nine selected isolates. In addition, fruits were inoculated with a conidial suspension and reproducible symptoms confirmed the presence of Colletotrichum fructicola in this area. This pathogenic species can now be added to those previously reported in the country, such as C. acutatum, C. boninense, C. godetiae, C. gloeosporioides, and C. karstii. Disease management programs to reduce the incidence of anthracnose should include C. fructicola to determine its response to fungicides that are routinely applied, considering that the appearance of new species is affecting the commercial quality of the fruits and shifting the original population structure.

• The Plant Pathology Journal
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• 제24권3호
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• pp.269-278
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• 2008

Ripe rot was frequently observed on fruits, leaves and stems of grape growing in eight locations in Korea from 2004 to 2006. All 30 isolates of Colletotrichum sp. were obtained from lesions of the ripe rot on grape plants. Out of the isolates, 19 isolates were identified as Colletotrichum acutatum and the others as Colletotrichum gloeosporioides based on morphological and cultural characteristics. Inter and intra specific variations of the Colletotrichum spp. isolates were investigated using RAPD and sequences of rDNA ITS and $\beta$-tubulin-2. Isolates of C. acutatum and C. gloeosporioides were distinctly differentiated by molecular analyses. Phylogenetic trees of ITS and$\beta$-tubulin-2 showed that Korean isolates of C. acutatum were clustered into groups A2 and A3 among the eight global groups. A2 included non-chromogenic isolates and A3 chromogenic ones. Both C. acutatum and C. gloeosporioides isolates were tested for pathogenicity to grape leaves. All isolates tested induced lesions on the leaves of grape by artificial inoculation. There was no difference in pathogenicity between C. acutatum and C. gloeosporioides isolates. This is the first report that C. acutatum except C. gloeosporioides causes grape ripe rot in Korea.

• 식물병연구
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• 제13권2호
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• pp.119-121
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• 2007

2004년부터 2005년까지 2년간 경남농업기술원 약초재배 포장에 재배중인 배암차즈기에서 이상증상이 발생하였다. 병징은 잎, 잎자루, 줄기 등 식물체 전부위에서 발생하였다. 식물체가 서서히 시들기 시작하면서 수침상으로 물러지고 썩으면서 갈색 또는 암갈색으로 변색되어 결국 말라 죽는다. 균총의 색깔은 감자한천배지에서 회색이며 배지표면에 분홍색의 분생포자퇴를 형성하였다. 분생포자의 모양은 단세포, 방추형이며, 크기는 $8{\sim}17{\times}3{\sim}4\;{\mu}m$이었다. 부착기는 연한 암갈색이고 곤봉상이며 크기는 $8{\sim}11{\times}4{\sim}6\;{\mu}m$이었다. 균사생육 적온은 $25^{\circ}C$였다. 이상의 결과로 보아, 이 병원균을 Colletotrichum acutatum J. H. Simmonds에 의한 배암차즈기 탄저병으로 명명할 것을 제안한다.

• 식물병연구
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• 제14권1호
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• pp.61-63
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• 2008

2007년 경상남도농업기술원 과수 포장에 재배중인 블루베리 과실에서 이상증상이 발생하였다. 병징은 성숙한 과실이 수침상으로 물러지고 부패되어지면서 낙과되어거나 미이라 상태로 남아 있으며 과실표면에 분홍색 포자퇴를 많이 형성하였다. 균총은 감자한천배지에서 회색이며 배지표면에 분홍색의 포자퇴를 많이 형성하였다. 분생포자의 모양은 단세포, 무색, 끝이 약간 무딘 원통형이며 크기는 $10{\sim}20{\times}3{\sim}5\;{\mu}m$이었다. 부착기는 갈색이고 곤봉형, 계란형이며 크기는 $6{\sim}18{\times}4{\sim}12\;{\mu}m$이었다. 균사생육 적온은 $25{\sim}30^{\circ}C$였다. 분리된 병원균으로 병원성을 검정한 결과, 자연 감염된 병징과 동일하였다. 이상의 결과를 가지고 본 병해를 Colletotrichum gloeosporioides에 의한 블루베리 탄저병으로 명명할 것을 제안한다.