• Title/Summary/Keyword: Coliform group

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Risk Assessment of Oyster Crassostrea gigas Processing Site For an HACCP System Model (HACCP 구축을 위한 굴(Crassostrea gigas) 가공공장의 위해 평가)

  • Kang, Kyung Tae;Kim, Min Joo;Park, Sun Young;Choi, Jong-Duck;Heu, Min Soo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.5
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    • pp.533-540
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    • 2016
  • This study assessed the risk of an oyster-shucking site to establish the hazard analysis critical control point (HACCP) system model by measuring viable cell counts, coliform group Staphylococcus aureus foreign material on oysters, oyster-producing equipment, and washing water. The viable cell count and coliform group levels of the harvested raw oysters were 4.00 log CFU/g and 1.1×102 MPN/100 g, while those of washed oysters were 2.99 log CFU/g and (3.2−4.6) × 10 MPN/100 g, respectively. After washing the oysters, no Escherichia coli or pathogenic bacteria (E. coli O157:H7, Listeria monocytogenes, S. aureus, Salmonella spp., Vibrio parahaemolyticus, and Clostridium perfringens) were detected. Regardless of the location of foreign matter, up to 100% more metallic and non-metallic foreign matter was detected at 1.5 mmΦ than at 3.5 mmΦ, using a metal detector with increased sensitivity. According to the results, the critical control points (CCP) are the washing and metal-detection processes. These results can be used as basic data to improve sanitation at oyster-shucking sites in factories with an HACCP system.

Effect of Physicochemical Properties of Cured Loin Ham by Inoculation of Lactobacillus plantarum(II) (Lactobacillus Plantarum이 염지 햄의 이화학적 특성에 미치는 영향(II))

  • Chung, Yung-Gun;Hyun, In-Hwan;Kim, Jong-Kyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.1
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    • pp.69-74
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    • 1986
  • In order to examine the effect of 'Lactobacillus plantarum' inoculation on the maturation of cured loin ham, bacteriological and biochemical changes in meat were investigated during curing periods. The results were summarized as follow; On the bacteriological changes of cured ham, during curing periods, the number of coliform group were decreased. while psychrotrophic and halo-tolerant bacteria were increased until the 4 days. In the brine solution after the 7days of the curing, the number of coliform group were decreased the 7 days, but psychrotophic and halo-tolerant bacteria were increased until the 7 days of curing. The pH value of the meat and curing solution were sharply decreased at the one day, since these were slightly increased from 4 days. The color development of cured meat was showed 84.05 % development of within the 7 days of curing. Glutamic acid contents among the 17 kinds of amino acid were the highest at the 7 and 10 days of curing. The 13 kinds of fatty acids detected from at the all sample and total contents of unsaturated fatty acid were slightly decreased during curing.

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A study on safety of functional healths foods (건강기능식품에 대한 안전성 조사연구)

  • Hwang, Won-Moo;Kim, Myeong-Hee;Yun, Ga-Ri;Lee, Gil-Bong;Go, Jone-Myeung;Kim, Yong-Hee
    • Korean Journal of Veterinary Service
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    • v.31 no.2
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    • pp.239-254
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    • 2008
  • This study was conducted in order to get basic data on standards and specifications of health and functional foods. A total of 101 kinds of functional healths foods were examined during the period of January to December 2005. Among them, 89 kinds were local products and 12 kinds were imported products. Test items were 6 kinds of heavy metals (lead, cadmium, mercury, copper, zinc and manganese), 5 bacteria (common bacteria, coliform group, E coli, Bacillus cereus and Clostridium perfringens), and 6 preservatives (sorbic acid, benzoic acid, DHA, ethyl paraben, propyl paraben and butyl paraben). As a result of heavy metals, cadmium, was detected from the glucosamine products by 1.52ppm in average, mercury from cereal products by 0.004ppm in average, and lead from chlorella foods by 3.48ppm in average. Bacteria were identified from 3 cereal products, and amount of common bacteria were about $4.8{\times}105cfu/g$ in average. E Coli and Coliform group were isolated from 2 products and 4 products, respectively. All of those products were flour meal products. Any of 6 kinds of preservatives was not detected from all the products. It is thought that these test results will be available as basic data for enactment of relevant laws and regulations for production and control of safer and more hygienic foods in the future because the standards of the harmful heavy metals are not complete or available yet according to the Food Sanitation Law and the Functional Healths Foods Law.

Sterilization of Garlic Powder by Irradiation (방사선(放射線)에 의한 마늘분말(粉末)의 살균(殺菌))

  • Kwon, Joong-Ho;Byun, Myung-Woo;Cho, Han-Ok
    • Korean Journal of Food Science and Technology
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    • v.16 no.2
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    • pp.139-142
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    • 1984
  • Effects of irradiation on the microbial growth and physicochemical properties of garlic powder were investigated during 3 months storage. Total bacteria and coliform group of garlic powder were $4.74{\times}10^{4}$ and $5.0{\times}10^{3}$ per g, respectively and irradiation of 5 kGy and 7 kGy could sterilize coliform group and total bacteria, respectively. $D_{10}$ value of total bacteria was 3.34 and no microorganisms were grown in 5 to f kGy irradiated groups after 3 months storage at $30{\pm}1^{\circ}C$. Moisture, sugars and pH of garlic powder were not remarkably changed during storage but pyruvic acid content was slightly decreased with storage period. Color difference of garlic powder after 3 months storage could not be distinguished by naked eye, but a slight change was recongized by the mechanical measurement.

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Seasonal Characteristics of Seawater Quality in the Suncheon Bay (순천만 수질환경의 계절적 특성에 관한 연구)

  • Jang, Sung-Guk;Cheong, Cheong-Jo
    • Journal of Wetlands Research
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    • v.12 no.2
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    • pp.47-57
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    • 2010
  • The purpose of this study is to investigate the seasonal variation of water quality parameters in Suncheon bay. We investigated at fifteen stations from October 2008to July 2009. picoplankton The salinity in the bay ranged from 8.8 to 31.9psu, DO ranged from 6.34 to 11.84 mg/L and the pH ranged from 7.80 to 8.34. The mean concentration of suspended solids ranged from 52.1 to 104.0 mg/L and COD concentration ranged from 2.27 to 5.33 mg/L. The total coliform group ranged from 6 to 37CFU. The concentrations of COD and total coliform group were relatively higher in the upper part of the Suncheon bay than lower one. The concentrations of nutrients such as nitrogen, phosphorus, silicate were also higher in the upper part of the bay, because the large amount of fresh water was inflowed into the bay from the Dong stream and Isa stream. Because the N/P ratio was ranged from 4.54 to 9.61 in this study, the limiting nutrients was nitrogen in the Suncheon bay comparing to the Redfield ratio.

Sterilization Effectiveness by Nominal and Absolute Filter in Pre-treatments of Honey (벌꿀의 전처리 공정 중 Nominal 및 Absolute Filter 적용을 통한 제균 효과에 관한 연구)

  • Kwon, Ki-Hyun;Cha, Hwan-Soo;Kim, Byeong-Sam;Sung, Jung-Min
    • Food Science and Preservation
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    • v.15 no.5
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    • pp.731-735
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    • 2008
  • The coliform group, Salmonella typhimurium and Staphylococcus were analyzed for eliminating of microorganism spore which could be embeded in honey and eradication of studied for heat resistance of thermoacidophilic bacteria was studied for marketing of honey after producing honey drink. The method for analyzing of heat resistance thermoacidophilic bacteria was membrane-seperated cell culture with $0.45\;{\mu}m$ micro-filter and vacuum aspirator. The results of bacteria, coliform group, Salmonella typhimurium and Staphylococcus was negative, but normal method such as sterilization with electrolyzed water, normal micro-filter, high-temperature heating and microwave did not have effect on heat resistance thermoacidophilic bacteria. Also, absolute type micro-filter of $0.45\;{\mu}m$ and $0.8\;{\mu}m$ microfilteration showed higher effect on heat resistance thermoacidophilic bacteria than micro-filter of normal type, showing negative results at all treatments.

Microbiological and Physicochemical Quality Characteristics of Low-salted Sauerkraut (fermented cabbage) (저염 Sauerkraut (fermented cabbage)의 미생물 및 이화학적 품질 특성)

  • Ji, Hye-In;Kim, Kyung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.37 no.1
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    • pp.61-72
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    • 2022
  • This study investigated the minimum salt concentration required for achieving the optimal quality characteristics of sauerkrauts made by adding 0.5, 1.0, 1.5, 2.0, and 2.5% (w/w) of sea salt to cabbage according to the fermentation period. For evaluating the quality characteristics, we measured the microorganisms (lactic acid bacteria, yeast, and coliform group), pH, total acidity, salinity, chromaticity, and hardness every 24 hours. The lactic acid bacteria were identified and analyzed, and acceptance test was carried out on the 4th day of fermentation. The results showed that the salinity of 0.5, 1.0, 1.5, and 2.0% sauerkrauts on the 4th day of fermentation was lower than the average salinity of Baechu-Kimchi. The 0.5, 1.0, 1.5, and 2.0% sauerkrauts had significantly higher lactic acid bacteria than the 2.5% sample, and the coliform group was not detected after the 5th day of fermentation. Among the microbes identified, Weissella cibaria JCM 12495 was found only in domestic sauerkraut, in addition to Lactococcus lactis NCDO 604, Leuconostoc citreum JCM 9698, and Lactobacillus sakei DSM 20017. The results of the acceptance test show that 1.0 and 1.5% sea salt sauerkraut had significantly higher overall acceptance compared to the other samples. In conclusion, sauerkraut with a salt concentration of 1.0 and 1.5% (w/w) had abundant lactic acid bacteria and excellent sensory properties, suggesting that the production of low-salted sauerkraut can be adopted to reduce consumer salt intake in the future.

Distribution of Microorganisms and Physico-Chemical Characteristics in the Chagwi-Do Coastal Waters, Jeju Island (제주 차귀도 연안해역 미생물 분포 및 이화학적 특성)

  • Moon Young-Gun;Kim Man-Chul;Lee Joon-Baek;Song Choon-Bok;Yeo In-Kyu;Kim Jong-Man;Park Guen-Tae;Son Hong-Joo;Choi Woo-Bong;Heo Moon-Soo
    • Journal of Environmental Science International
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    • v.15 no.3
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    • pp.271-278
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    • 2006
  • To investigate the variations of physico-chemical factors and microbial population, in ten stations at water region of coastal area of Chagwi-Do, Nutritive salts, water temperature, transparency, suspended solid, salinity, COD, DO, pH, heterotrophic bacteria, coliform group and Vibrio spp. were analysed three times in September, November in 2004 and February in 2005. Heterotrophic bacteria in surface water was $3.5X10^1{\sim}1.16X10^3cfu/ml,\;1.0X10^2{\sim}5.2X10^1cfu/ml\;2.0X10^1{\sim}7.6X10^1cfu/ml$ and bottom water counted $7.0X10^2{\sim}1.0X10^3cfu/ml,\;1.4X10^1{\sim}2.5X10^2cfu/ml\;2.0X10^2{\sim}4.2X10^1cfu/ml$ in September, November in 2004 and February in 2005, respectively. The cell number of total coliform bacteria in the surface water amounted to $0{\sim}4.3X10^2cfu/ml,\;0{\sim}6.0X10^1cfu/ml,\;0{\sim}1.0X10^1cfu/ml$ and bottom water amounted $0{\sim}2.2X10^2cfu/ml,\;0{\sim}5.4X0^2cfu/ml,\;0{\sim}2.0X10^1cfu/ml$ in September, November in 2004 and February in 2005, respectively. As for Vibrio spp., the cell number in the surface water was $1.0X10^1{\sim}2.5X10^2cfu/ml,\;1.0X10^1{\sim}2.0X10^1cfu/ml,\;0cfu/ml$ and bottom water counted $1.0X10^1{\sim}5.2X10^2cfu/ml,\;0cfu/ml,\;2.0X10^1cfu/ml$ in September, November in 2004 and February in 2005, respectively.

Effects of Dietary Probiotic Mixture on Caecal Microflora, Immune Response, Egg Quality and Production of Layerunder Heat Stress (혼합 생균제가 열 스트레스에 노출된 산란계의 생산성, 계란품질, 면역반응 및 맹장 미생물에 미치는 효과)

  • Song, Young-Han;Goh, Yong Gyun;Um, Kyung-Hwan;Park, Byung-Sung
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.3
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    • pp.787-796
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    • 2018
  • This study was conducted to investigate the effects of probiotic mixture on fecal ammonia, caecal microorganism, immune response, egg quality and production in layer under heat stress (HS).A total of four hundred 50 week olds Hy-Line Brown layers were randomly divided into four groups of 100 heads each: C (control, room temperature $25^{\circ}C$), HS (heat stress $33^{\circ}C$), PM (HS plus probiotic mixture 500, 750 mg/kg of diets). Egg production, egg quality, spleen weight, blood IgG and lymphocyte concentrations were increased in the PM group compared to the HS group, while mortality, the heterophil:lymphocyte (H:L) ratio, and corticosterone levelswere significantly decreased. Lactobacillus was increased in the PM group compared to the HS group, but E. coli, coliform bacteria and aerobic bacteria were significantly reduced. Fecal ammonia production was significantly increased in the HS group compared to the PM group. In conclusion, the results of this study that these mixed probiotics can reduce the heat damage of the summer laying hens and can be an effective nutritional strategy to reduce odor generation from feces, and to improve egg quality and laying production through immune response and caecal microbial balance.

Microbiological Study of the Extracorporeal Circulation (체외순환법에 대한 미생물학적 고찰)

  • 조대윤
    • Journal of Chest Surgery
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    • v.13 no.1
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    • pp.48-51
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    • 1980
  • Open heart surgery patients have additional risks arising from the special nature of the operative procedure. And postoperative infections in patients with extracorporeal circulation are associated with high incidence of serious sequelae. To investigate the incidence and organisms of contamination, and the relation between the duration of extracorporeal circulation and contamination, following study was done. Eighty-four of the open heart surgery patients were examined with cultures from the blood and priming solution before and after bypass. 1. Cultures before bypass were sterile, but 2 cases of cultures from the blood and priming solution after bypass were positive, and culture positive group was 5% of all patients. 2. The organisms were Gram-negative, aerobic coliform bacilli; 3. The culture positive group had significantly longer bypass time.

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