• BMB Reports
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• 제40권6호
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• pp.952-958
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• 2007

We isolated many genes induced from pepper cDNA microarray data following their infection with the soybean pustule pathogen Xanthomonas axonopodis pv. glycines 8ra. A full-length cDNA clone of the Capsicum annuum ankyrin-repeat domain $C_3H_1$ zinc finger protein (CaKR1) was identified in a chili pepper using the expressed sequence tag (EST) database. The deduced amino acid sequence of CaKR1 showed a significant sequence similarity (46%) to the ankyrin-repeat protein in very diverse family of proteins of Arabidopsis. The gene was induced in response to various biotic and abiotic stresses in the pepper leaves, as well as by an incompatible pathogen, such as salicylic acid (SA) and ethephon. CaKR1 expression was highest in the root and flower, and its expression was induced by treatment with agents such as NaCl and methyl viologen, as well as by cold stresses. These results showed that CaKR1 fusion with soluble, modified green fluorescent protein (smGFP) was localized to the cytosol in Arabidopsis protoplasts, suggesting that CaKR1 might be involved in responses to both biotic and abiotic stresses in pepper plants.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1227-1232
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• 2008

Carcass characteristics data of Hanwoo (N = 1,084) were collected from two stations of the National Livestock Institute of Animal Science (NIAS), Korea and records from thirteen individual cow-calf operators were analyzed to estimate variance and covariance components and the effect of different slaughter endpoints. Carcass traits analyzed were cold carcass weight (CWT, kg), REA (rib eye area, cm2), back fat thickness (mm) and marbling score (1-7). Four different models were examined. All models included sex and contemporary group as fixed effects and the animal's direct genetic potential and environment as random effects. The first model fitted a linear covariate of age at slaughter. The second model fitted both linear and quadratic covariates of age at slaughter. The third model fitted a linear covariate of body weight at slaughter. The fourth model fitted both linear covariates of age at slaughter and body weight at slaughter. Variance components were estimated using the REML procedure with Gibb's sampler. Heritability estimate of CWT was in the range of 0.08-0.11 depending on the model applied. Heritability estimates of BF, REA and MS were in the ranges of 0.23-0.28, 0.19-0.26, and 0.44-0.45, respectively. Genetic correlations between CWT and BF, between CWT and REA, and between CWT and MS were in the ranges of -0.33 - -0.14, 0.73-0.84, and -0.01- 0.11, respectively. Genetic correlations between REA and BF, between MS and BF and between REA and MS were in the ranges of -0.82 ~ -0.72, 0.04~0.28 and -0.08 ~ -0.02, respectively. Variance and covariance components estimated varied by model with different slaughter endpoints. Body weight endpoint was more effective for direct selection in favor of yield traits and body weight endpoints affected more of the correlated response to selection for the traits of yield and quality of edible portion of beef.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2007년도 International Meeting of the Microbiological Society of Korea
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• pp.83-85
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• 2007

Deep-sea bacteria are adapted to extreme environments, such as high pressures and cold temperatures. We have isolated many piezophiles which grow well even under high pressures from deep-sea sediment. Shewanella violacea DSS12 and Moritella japonica DSK1 have the ability to grow at up to 70 MPa, and those bacteria have unique mechanisms of gene expression in response to high pressure conditions. The combination of gene expression systems in piezophiles, like the high pressure-dependent promoters and GFP reporter gene, may reveal highly fluorescent cells when exposed to high hydrostatic pressure conditions. It is predicted that a novel bio-sensing system can be made to probe high pressure environments using living bacteria. First, gene transformation into our piezophiles, strains DSS12 and DSK1, were examined. Eschericha coli S17-1 was used for bacterial conjugation with those piezophiles. As a result, the broad host range vector, pKT231, and the shuttle vector, pTH10, were successfully introduced to DSS12 and DSK1, respectively. Next, The pressure regulated promoters from DSS12 and DSK1 were cloned into proper vectors and combined with GFP as a reporter gene downstream of each promoter. The transformants of DSK1 and DSS12 with the recombinant pTH10 and pKT231 plasmid, which has cadA and glnA promoters (each of them is a pressure regulated promoter from DSK1 and DSS12, respectively) and GFP, were grown under high pressure and gene expression of GFP promoted by 50 MPa pressure was confirmed. This is a critical point to create a pressure-sensing bacteria, as the "High Pressure Glow Cells", which will indicate the level of environmental pressure using fluorescence of GFP as a reporter gene.

• Journal of Biosystems Engineering
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• 제19권4호
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• pp.358-368
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• 1994

Major factors in reducing the stacking strength of corrugated fiberboard boxes in cold storage or transport conditions are high relative humidity, causing elevated moisture absorption by the boxes. The bottom boxes in a stack will deform to the critical deflection causing agricultural products damage there, and eventually additional deflection will cause box collapse and finally toppling of the stack. The study was conducted to determine the water absorption characteristics and the compressive strength of the corrugated fiberboard boxes being widely used in packaging agricultural products in Korea. The sample boxes for the study were selected from the regular slotted containers (RSC) types, and one was the box used in apple packaging (Box A), another one was the box used in pear packaging (Box B). The corrugated shipping containers were made from a large portion of recycled fibers in Korea, and comparing with Box B, Box A was fabricated from fiberboard which contained more percentage of old corrugated containers (OCC) imported from foreign countries than domestic waste paper. The results obtained from the study were summarized as follows ; 1. Equilibrium moisture content (EMC) of the sample boxes was established after about 20 hours, and the EMC by absorption was lower than that by desorption. The EMC increased with the increasing of relative humidity and with the decreasing of temperature, and the rate of increasing was much higher above the relative humidity of 50%. 2. The maximum compressive strength of Box A was about 100 kgf greater than that of Box B on the same enviromental conditions. The strength of the sample boxes decreased rapidly with the increasing of relative humidity. The effect of relative humidity on the strength was a little higher than that of temperature. 3. As the applied load was progressively increased and a level was reached, the vertical side panels ($L{\times}D$) deflected laterally inwards or outwards. The panels deflected laterally inwards at higher relative humidity. 4. The maximum compressive deflection ratio and the critical deflection ratio of the sample boxes were increased linearly with the increasing of relative hunidity, but trends for its ratios showed inconsistant response to temperature.

• 동의생리병리학회지
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• 제25권4호
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• pp.663-668
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• 2011

Galgeun-tang (GGT) has been a great source for treating cold diseases in the folk medicine recipe. Carbon tetrachloride ($CCl_4$) is one type of hepatotoxin that can eventually cause liver injury. During the experiment, we first studied the protective effects of GGT against $CC_4$-induced hepatotoxicity. GGT was pretreated for 3 h, and 1% $CCl_4$ was added to mouse primary liver cells. After 4 h, ROS generation and expression of antioxidant enzymes (catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)) were analyzed by FACS and real time PCR. Also, the activities of ALT and LDH were measured using cultured medium. The hepatic levels of TNF-alpha and iNOS, which are related to inflammation and stress response gene, HSP72 and HO-1 were analyzed by PCR or real time PCR. Liver tissues were analyzed by HE stain. From the observation, we discovered that GGT treatment protects $CCl_4$-induced hepatotoxicity, and that GGT pretreatment decreases ROS generation, TNF-alpha and iNOS expression. However, gene expression of CAT, SOD, GPx, HSP72 and HO-1 were increased by GGT. These results lead to the conclusion that GGT has protective effects against $CCl_4$-induced hepatotoxicity.

• 자원환경지질
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• 제36권6호
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• pp.545-556
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• 2003

영산강 하구 퇴적물에서 최종빙기 및 현세에 해당하는 길이 18.9m의 시추 퇴적물(심도 20.5∼l.6m)을 획득하였으며, 퇴적물의 조직과 유기질 미화석 분석을 통하여 고환경의 변화를 인지하였다. AMS$^{14}$C 연대측정과 한반도에서의 화분분대 대비에 의하여 퇴적물 연대를 결정하였다. 본 시추 퇴적물에서는 크게 3가지 고환경대를 구분할 수 있었다. (1)최종빙기 퇴적물:주로 하천 퇴적층이며 고토양화 되어 있다. 퇴적 작용과 토양화 작용을 수차례에 걸쳐 받은 것으로 보이며, 고토양의 양상으로 보아 한랭$.$습윤 환경에서 토양화 작용이 진행된 것으로 추측할 수 있다. (2) 현세 초기∼중기 퇴적물:해수면 상승으로 인하여 해수의 영향을 받게 되었으며, 해양성 미화석을 다량 포함하는 점토로 구성된다. (3) 현세 후기 퇴적물: 보다 한랭한 기후를 나타낸다. 이러한 양상은 지역적, 전지구적 환경변화 양상과 일치하는 결과를 보인다.

• 한국항공우주학회지
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• 제32권7호
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• pp.126-132
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• 2004

위성 추진제 탱크의 열제어는 써모스탯에 의해 작동되는 히터를 이용해 수행된다. 적절한 위치에 써모스탯을 부착하는 것이 관건이지만 구형 탱크에서는 부착위치를 수치적으로 정확하게 부여하는 것이 불가능하다. 실제로는 도면에 대략적으로 위치를 제시한 후 작업자의 경험과 판단에 의존하여 써모스탯을 부착하는 것이 현실이다. 그러므로 써모스탯의 부착위치에 대한 민감도 해석을 수행함으로써 그에 따른 탱크의 열적 거동 및 열제어에 미치는 영향을 정량적으로 파악하였다. 실제 탱크 모듈 조립시 써모스탯의 부착위치에 대한 오차가 존재하더라도 설계 도면에 명시된 값보다 크게 벗어나지 않는 한 탱크의 열제어 성능은 충분히 보장되리라 판단된다.

• 원예과학기술지
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• 제32권5호
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• pp.684-693
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• 2014

저온, 건조, 염과 같은 비생물적 스트레스는 식물의 생리적 형태적 변화와 수확량 감소를 초래한다. 이러한 이유로 식물체는 불리한 환경을 극복하기 위해 다양한 대사과정에 관련된 유전자들간의 복잡한 상호 관계를 조절함으로써 저항성을 획득한다. 본 연구는 배추에서 염 스트레스에 반응하는 유전자를 다각적으로 분석하기 위해 상호발현 네트워크를 구축하였다. 네트워크를 구축하기 위하여 배추를 염스트레스 조건 하에서 시간 경과에 따라 KBGP-24K 마이크로어레이 분석을 실시한 [BrEMD (Brassica rapa EST and Microarray Database)] 실험 결과를 수집하여 분석하였다. 구축된 네트워크 모델은 1,853개 node, 5,740개 edge, 및 142개 connected component(상관계수 > 0.85)로 구성되었다. 구축된 네트워크 분석 결과, ROS 신호 전달을 통한 N$Na^+$ 수송활성화와 proline 축적이 배추의 염 저항성 획득과 밀접한 연관이 있는 것으로 판단하였다.

• Child Health Nursing Research
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• 제15권3호
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• pp.271-281
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• 2009

Purpose: This study was conducted to develop an instrument to measure coping in emergency situations. Methods: The process included construction of a conceptual framework, generation 60 primary items, verification of construct validity and extraction of final items. The.60 preliminary items were reviewed for content validity by seven experts and were tested to evaluate inter-item correlation coefficient by three groups of elementary school students. From November 1 to December 10, 2008, data were collected from 920 elementary school students according to residential characters (major city, small town, rural area). Item analysis, factor analysis with rotation Varimax and Cronbach's alpha were used to analyze the data. Results: 1) There were 45 items in the final instrument categorized into 8 factors. 2) The factors were labeled as "Call for help and check" (8 items), "Care for common symptoms" (6 items), "Appropriate response for the situation" (5 items), "Evacuation" (6 items), "Care for tissue damage" (8 items), "Care for injury by heat, cold or foreign body" (6 items), "Offer help" (4 items) and "Rescue & escape" (2 items). 3) Cumulative percent of variance was 53.10% and eigen values ranged from 1.04 to 13.38. 4) Cronbach's alpha for the total was .943 and ranged from .527 to .869. Conclusion: Validity and reliability of the scale are confirmed in this study showing its utility for measuring coping in emergency situations with elementary school students. Utilization of the scale will also contribute to designing appropriate coping education programs for elementary school students.

• 한국자원식물학회지
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• 제27권4호
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• pp.392-399
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• 2014

본 연구에서는 식물의 노쇠와 관련된 SENESCENCE 1 (SEN1) 유전자를 현사시나무에서 분리하고, 여러 가지 조건에서 발현 특성을 분석하였다. 현사시나무의 SEN1 유전자(PagSEN1)는 243개의 아미노산으로 이루어져 있고, 한 개의 rhodanese domain을 가지고 있다. Southern blot 분석 결과 PagSEN1 유전자는 현사시나무에서 2 copy 정도가 존재하는 것으로 나타났다. 조직 특이적 발현양상을 분석한 결과 PagSEN1 유전자는 성숙잎에서 가장 높게 발현하고, 뿌리에서 가장 낮게 발현하는 것으로 나타났다. 또한 mannitol과 염 스트레스 처리에 의해 300배 이상 발현이 증가한 반면에 저온 스트레스에는 반응하지 않았다. 식물 호르몬 처리에서는 ABA와 JA 처리 10시간 후에 발현이 3.5배와 2.4배 이상 증가하는 것으로 나타났다. 따라서 PagSEN1 유전자는 건조와 염 스트레스에 반응하며, 식물의 자연적 노쇠과정 뿐 아니라 스트레스와 같은 환경변화에 의해 유발되는 노쇠과정에도 관여하는 것으로 판단된다.