• 한국가스학회지
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• 제24권4호
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• pp.56-61
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• 2020

수소의 액화에는 예냉 에너지, 상변화 에너지, 수소 변환열 제거 등 다량의 에너지가 요구되어진다. 본 논문의 목적은 예냉공정에 필요한 에너지로 LNG냉열로 액체질소를 제조하여 사용하는 LNG냉열 간접 이용 방식과, Cold box의 단열에 냉공기를 이용하는 새로운 에너지절약 공정을 제안하여 수소액화 수율을 향상시키고자 하였다. 분석 결과를 보면, LNG냉열 간접이용 방식은 에너지 절약과 함께 액체수소 플랜트의 안전성을 제공하는 장점을 갖는다. 새로운 Cold box 단열 방식은 외벽 철판 3mm/우레탄폼 20cm/공기 5cm/우레탄폼 20cm/설비의 구조일 때 현재 펄라이트 단열에 비교하여 열유입량이 약 35%~50%가 감소하게 된다. 또한 냉공기 보다 온도가 높은 설비는 냉각의 효과를 얻게 된다. 수소액화 플랜트의 공정에 본 결과를 적용한다면 액체 수율이 50% 내외로 크게 향상되는 효과를 제공하게 된다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2011년도 춘계학술대회 논문집
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• pp.1215-1216
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• 2011

• 한국수소및신에너지학회논문집
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• 제31권6호
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• pp.571-577
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• 2020

Thermal analysis was performed for a cold box of a hydrogen liquefaction pilot plant with 0.5 ton/day capacity. The pilot plant has adopted a hydrogen liquefaction process using two-stage helium Brayton cycle with precooling of liquid nitrogen. The cold box for hydrogen liquefaction has generally vacuum insulation but inevitable heat invasion by conduction and radiation exists. The heat loads were calculated for cold box internals according to multilayer insulation emissivity. Total heat load of 181.7 W is estimated for emissivity of 0.03 considered in field condition.

• 한국초전도ㆍ저온공학회논문지
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• 제24권4호
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• pp.65-70
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• 2022

A vacuum system is designed for thermal insulation of a 10 ton/day class air liquefaction cold box for liquid air energy storage. The vacuum system is composed of a turbomolecular pump, a backing pump and vacuum piping for the vacuum pumps. The turbomolecular pump is in combination with the backing pump for pumping capacity. The vacuum piping is designed with system installation conditions, such as distance from the cold box, connections to vacuum pumps and installation space. The capacity of the vacuum pump combination, namely pumping speed, is determined by analysis of the vacuum system, and pump-down time to 1×10^-5 mbar is estimated. Vacuum piping conductance, system pumping speed and outgassing rate are calculated for the pump-down time with the ultimate pumping speed range of the vacuum pump combination of 1400 - 2300 l/s. Although the pump-down time gets shorter by larger capacity vacuum pumps, it mainly depends on target vacuum degree and outgassing rate in the cold box. The pump-down time is estimated as 3 - 6 hours appropriate for cold box operation for the pumping speed range. Considering the outgassing rate has uncertainty, the vacuum pump combination with pumping speed of 1900 l/s is chosen for the vacuum system, which is middle value of the pumping speed range.

• 융복합기술연구소 논문집
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• 제2권1호
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• pp.1-6
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• 2012

The cold chain system in South-East Asia is requiring to maintain freshness of refrigerated or frozen food. In this study, Thermal storage system using Phase change material (PCM) was developed and evaluated its performance about temperature and cold keeping time. For various application of cold chain system, we developed portable cold box, cold roll container and freezing station. Keeping time on laboratory tests of portable cold box in case of refrigeration and freezing were 6 hours and 4 hours, respectively. Cold container was developed to 2.5 ton scale. Evaluation in Indonesia, it was showed to keep the setting temperature of $-10^{\circ}C$ over 40 hours at $30^{\circ}C$ of ambient air. Freezing station using PCM was kept over 24 hours under $-20^{\circ}C$.

• 미생물학회지
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• 제47권4호
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• pp.289-294
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• 2011

Bacillus subtilis에 존재하는 DEAD-box RNA helicase 유전자의 결손이 저온 충격에 민감성을 보이는지를 조사하였다. 저온 충격에 민감한지를 알아 보기 위하여 대수기에($O.D_{600}$=0.5-0.6) 있는 세포를 $15^{\circ}C$도 낮추어 저온충격을 가하여 생장하는 정도를 조사하였다. DEAD-box RNA helicase 유전자 ydbR, yfmL, yqfR, deaD의 결손 균주들이 저온충격을 가하였을 때 ydbR 결손 균주의 생장이 야생형 균주에 비해 5배 정도 현저히 감소하였으나, 다른 DEAD-box RNA helicase 유전자의 (yfmL, yqfR, deaD) 결손은 야생형 균주와 비슷한 생장을 보였다. 저온에서의 유전자 발현을 알아보기 위하여 Northern blot으로 mRNA 양을 알아본 결과 $37^{\circ}C$에 비해 $15^{\circ}C$에서 ydbR과 yqfR의 mRNA전사물 증가를 확인할 수 있었고, 반면에 yfmL과 deaD의 전사 증가는 관찰되지 않았다. $37^{\circ}C$에서 $15^{\circ}C$로 저온 충격을 가하면 ydbR mRNA 양의 뚜렷한 증가를 확인하였고, 전사 억제제인 rifampicin를 처리하여 ydbR mRNA의 양을 조사하였을 때 $15^{\circ}C$ 조건에서는 mRNA 양이 거의 유지하는 반면에 $37^{\circ}C$ 조건에서는 급격한 mRNA의 감소가 일어나 전사과정에서 유도되기 보다는 전사 후 전사물의 안정에 기인하는 것으로 보인다. 이와 관련하여 ydbR 유전자의 5' UTR (untranaslated region) 부근에서 csp (cold shock protein) 유전자에서 관찰되는 cold box element를 확인하였고, ydbR이 저온 충격 조건에서 발현되는 과정이 csp와 유사하게 전사물의 안정성에 기인함을 알 수 있었다.

• Molecules and Cells
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• 제39권3호
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• pp.250-257
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• 2016

Abiotic stresses such as drought and low temperature critically restrict plant growth, reproduction, and productivity. Higher plants have developed various defense strategies against these unfavorable conditions. CaPUB1 (Capsicum annuum Putative U-box protein 1) is a hot pepper U-box E3 Ub ligase. Transgenic Arabidopsis plants that constitutively expressed CaPUB1 exhibited drought-sensitive phenotypes, suggesting that it functions as a negative regulator of the drought stress response. In this study, CaPUB1 was over-expressed in rice (Oryza sativa L.), and the phenotypic properties of transgenic rice plants were examined in terms of their drought and cold stress tolerance. Ubi:CaPUB1 T3 transgenic rice plants displayed phenotypes hypersensitive to dehydration, suggesting that its role in the negative regulation of drought stress response is conserved in dicot Arabidopsis and monocot rice plants. In contrast, Ubi:CaPUB1 progeny exhibited phenotypes markedly tolerant to prolonged low temperature ($4^{\circ}C$) treatment, compared to those of wild-type plants, as determined by survival rates, electrolyte leakage, and total chlorophyll content. Cold stress-induced marker genes, including DREB1A, DREB1B, DREB1C, and Cytochrome P450, were more up-regulated by cold treatment in Ubi:CaPUB1 plants than in wild-type plants. These results suggest that CaPUB1 serves as both a negative regulator of the drought stress response and a positive regulator of the cold stress response in transgenic rice plants. This raises the possibility that CaPUB1 participates in the cross-talk between drought and low-temperature signaling pathways.

• 한국초전도저온공학회:학술대회논문집
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• 한국초전도저온공학회 2001년도 학술대회 논문집
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• pp.132-135
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• 2001

Cryogenic air separation unit(ASU) was developed about 100 year ago in Europe. However, because there is not any ability of process design or manufacturing of ASU in Korea, many ASUs come from advanced countries every year. The purpose of this study is the development of cryogenic air separation unit by our own ability, especially cold box for nitrogen production. On this study, we developed the computer program for physical properties of gases and process simulation. We also did process design and manufactured of cold box, including air separation column, liquid air heat exchanger and condenser. The result of cold box test was successful.

• 생물환경조절학회지
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• 제11권2호
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• pp.81-87
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• 2002

근권냉방 방식에 따른 냉방 효율과 토마토 생육 특성을 검토하고자 XL냉수순환, 라디에이타 냉풍, 패드냉풍 및 대조구를 처리한 결과는 다음과 같다. 근권부 냉방온도는 공급수온이 17.8$^{\circ}C$일 때 XL 냉수순환구는 18.$0^{\circ}C$ 라디에이타 냉풍구는 27.2$^{\circ}C$, 패드 냉풍구는 20.3$^{\circ}C$였다. 배지 깊이와 시간대별 평균온도는 XL냉수순환구 22.8~24.7$^{\circ}C$. 라디에이타 냉풍구 22.7~24.2$^{\circ}C$, 패드 냉풍구 20.5~23.2$^{\circ}C$, 대조구 25.9~29.6$^{\circ}C$를 나타내어 냉방처리구가 대조구에 비해 5~6$^{\circ}C$더 낮았다. 토마토의 생육에 있어 엽장. 엽폭은 대조구에 비해 컸으며, 생체중 및 건물중의 경우에서도 비슷한 경향이었다 근활력에 있어 대조구는 45.0ug/g에 비해 근권냉방 처리구는 58.5-62.8 ug/g으로 높았으나. 근권냉방 처리구간에는 큰 차이가 없었다.

• 한국초전도ㆍ저온공학회논문지
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• 제15권4호
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• pp.6-9
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• 2013

Korea Superconducting Tokamak Advanced Research (KSTAR) has a helium refrigeration system (HRS) with the cooling capacity of 9 kW at 4.5 K. Main cold components are composed of 300 tons of superconducting (SC) magnets, main cryostat thermal shields, and SC current feeder system. The HRS comprises six gas storage tanks, a liquid nitrogen tank, the room temperature compression sector, the cold box (C/B), the 1st stage helium distribution box (DB#1), the PLC base local control system interconnected to central control tower and so on. Between HRS and cold components, there's another distribution box (DB#2) nearby the KSTAR device. The entire KSTAR device was constructed in 2007 and has been operated since 2008. This paper will present the maintenance result of the KSTAR HRS during the campaign and discuss the operation record and maintenance history of the KSTAR HRS.