• 환경생물
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• 제20권3호
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• pp.237-244
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• 2002

항진균 활성 관련 단백질을 탐색하기 위해 미역류로부터 식물병원성 곰팡이의 생장을 저해하는 SAR01 균주를 분리하였고, FAME (fatty acid methyl ester) 분석 결과, Streptomyces sp.로 동정되었다. 방사선 조사$(^{60}Co)$를 실시한 결과, Botrytis cinerea를 포함한 5종의 식물병원성 곰팡이에 대한 항진균 활성을 소실한 SAR535 균주 외 6종의 돌연변이 균주가 유도되었다. SAR01 야생형 균주와 SAR535 돌연변이 균주의 세포내 단백질의 이차원 전기영동 분석결과, 6종의 단백질이 야생형 균주인 SAR01 균주의 세포내에만 존재하였다. 이들 6종의 단백질 중, 5종은 heat shock protein 70 (HSP70), Fe-containing superoxide dismutase II (Fe - SODII), ribosome recycling factor (RRF), 10 kD chaperonin (GroES) 및 inorganic pyrophosphatase (PPAse)와 각각 75%, 93%, 100%, 96% 및 83%의 유사성을 보였다. 이들 6종의 단백질들은 Streptomyces sp. SAR01 균주의 항진균 활성과 밀접한 관계가 있을 것으로 사료된다.

• 미생물학회지
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• 제39권3호
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• pp.141-147
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• 2003

여러 항진균 활성 관련 유전자들의 발현 수준을 동시에 연구하기 위하여 DNA microarray를 이용하여 유전자들의 발현 패턴을 비교 분석하였다. 본 연구에서는 항진균활성을 가지는Bacillus lentimorbus WJ5의 genomic DNA를 무작위 하게 제한효소로 절단하여 2,000개의 DNA단편을 microarray하였으며, 감마선($^{60}Co$)조사로 유도된 7종의 항진균 활성 결핍 돌연변이체와 발현양상을 정량적으로 비교하였다. Gene Cluster (Michael Risen, Stanford Uniy.)를 이용한 DNA microarray의 분석 결과, 총 408개의 DNA 단편이 발현되는 것을 확인할 수 있었으며, 이들 중 20개의 DNA단편이 항진균 활성 결핍 돌연변이체에서 발현이 억제되는 것으로 나타났다. 특히,pbuX (xanthine permease, K222), ywbA (phosphotransferase system enzyme II, K393), ptsG (PTS glucose specific enzyme II ABC component, K877), yufO (ABC transporter(ATP-binding protein), K1301), 그리고 ftsY (signal recognition particle (docking protein), K868)는 모든 돌연변이체에서 동시에 발현되는 down-regulation된 유전자들로서 물질 이동과 관련된 것으로 보고되어 있으며, 항진균 활성 관련 신호 및 물질의 이동에 관여할 것으로 사료되어진다.

• 대한수의학회지
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• 제44권1호
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• pp.41-47
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• 2004

The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of $^{60}Co$ ${\gamma}$-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p<0.01) and TL (p<0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs. Mouse PBLs were more sensitive than human PBLs which were in turn more sensitive than rat PBLs when the treated dosages were 1 and 2 Gy. However, human PBLs were more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs when the irradiation dosages were 4 and 8 Gy. Data from all three species could be fitted to a linear-quadratic model. These results indicated that there may be inherent differences in the radio-sensitivity among PBLs of mammalian species.

• Nuclear Engineering and Technology
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• 제56권9호
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• pp.3925-3932
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• 2024

During outages at nuclear power plants, much more care for radiation workers against internal exposure should be ensured given that more hot particles exist relative to the amount during normal operation. If fuel-type hot particles (FTHP) are inhaled, they can cause more severe health risks compared to activation-type hot particles (ATHP), which contain ⁶⁰Co, due to the alpha-emitting nuclides within FTHPs. The activities of difficult-to-measure nuclides within FTHPs inhaled by workers are inferred by the age-dating technique using a¹⁴¹Ce/¹⁴⁴Ce ratio as measured by whole-body counters. However, this method may be limited to outages that last for only a few months due to the short half-life (32.5 days) of ¹⁴¹Ce. We studied the feasibility of utilizing ²⁴¹Am, a nuclide with a long half-life of 432.6 years, as an alternative to ¹⁴¹Ce. Additionally, we improved the performance of a stand-type whole-body counter for low-energy gamma spectroscopy to meet the criterion (RMSE ≤0.25) specified in ANSI/HPS N13.30-2011 by employing an artificial neural network (ANN). This study can contribute to more rapid and accurate internal dose assessments for workers who have inhaled FTHPs during long-term outages at nuclear power plants.

• Journal of Ginseng Research
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• 제17권1호
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• pp.29-34
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• 1993

This study was undertaken to investigate the effect of red ginseng extract (5.5 mg/mouse ip) on the activities of superoxide dismutase (SOD), peroxidase and catalase in the liver of the gamma ray irradiated male mice. The experimental groups consisted of control, red ginseng extract injection group, irradiation (8 $Gy^{60}$Co) group and red ginseng extract injection after irradiation group. in red ginseng extract injection group, SOD, peroxidase and catalase activities were similar to that in the control group. In irradiation group SOD, peroxidase and catalase activities increased progressively until the 2nd day after the treatment and then decreased thereafter, whereas red ginseng extract injection after irradiation group recovered more rapidly than irradiation group. The above results suggested that red ginseng extract injection after irradiation group have the recovery effects on the activities of SOD, peroxidase and catalase after radiation injury in the liver of male mice.

• Journal of Dairy Science and Biotechnology
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• 제28권2호
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• pp.13-18
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• 2010

방사선 조사된 완제품 조제분유를 포함한 시료 4종의 ESR 스펙트럼 특성을 측정하였다. 조제분유와 전지분유의 비조사 시료는 g=2.0050의 아주 약한 singlet 형태의 스펙트럼을 나타내었다. 조사 시료에서 전지분유는 g=2.0050의 singlet 형태의 스펙트럼, 조제분유는 전지분유와 같은 중심 피크 옆에 g=2.0006의 작은 피크를 가진 비대칭 형태의 스펙트럼을 나타내었고, 두 시료 모두 비조사 시료와 조사 시료의 신호 세기 차이가 명확하여 조사유무 판별이 가능하였다. 포도당과 유당의 비조사 시료는 아무런 시그널을 나타내지 않았으며, 조사 시료는 이미 알려져 있는 결정형 당 유래의 다형 피크를 가진 스펙트럼을 나타내었다. 선량이 증가함에 따라 신호 세기도 증가하였고, 회귀방정식의 상관계수는 0.95 이상의 높은 값을 나타내었다. 감마선과 전자선 조사에 의한 ESR 스팩트럼 간의 유의성 있는 차이는 없는 것으로 확인되었다. 결론적으로 본 실험을 통하여 ESR 분석은 방사선 조사된 조제분유 및 원료의 판별에 유용한 방법으로 적용될 수 있는 것으로 판단되었으며, 온도, 습도 및 전처리 조건 등의 요인에 의한 변화 가능성과 식품 생산 및 장기유통 기간 등을 고려할 때 이에 대한 지속적인 연구와 자료 축적이 요구된다고 할 것이다.

• Nuclear Engineering and Technology
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• 제55권11호
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• pp.4213-4227
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• 2023

Computer Automated Radioactive Particle Tracking (CARPT) technique has been successfully utilized to measure the velocity profiles and mixing parameters in different multiphase flow systems where a single radioactive tracer is used to track the tagged phase. However, many industrial processes use a wide range of particles with different physical properties where solid particles could vary in size, shape and density. For application in such systems, the capability of current single tracer CARPT can be advanced to track more than one particle simultaneously. Tracking multiple particles will thus enable to track the motion of particles of different size shape and density, determine segregation of particles and probing particle interactions. In this work, a newly developed Multiple Radioactive Particle Tracking technique (M-RPT) used to track two different radioactive tracers is demonstrated. The M-RPT electronics was developed that can differentiate between gamma counts obtained from the different radioactive tracers on the basis of their gamma energy peak. The M-RPT technique was validated by tracking two stationary and moving particles (Sc-46 and Co-60) simultaneously. Finally, M-RPT was successfully implemented to track two phases, solid and liquid, simultaneously in three phase slurry bubble column reactors.

• 전기의세계
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• 제26권2호
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• pp.89-94
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• 1977

In order to investigate the properties of radiation resistance together with dielectric, and mechanical relaxation behaviors of polyvinylcholoride exposed to several different doses under the .gamma.-ray of Co$^{60}$ source, several observations were carried out on the exposed specimens propared by mixing dibutyl-tin-dilaulate and dibutyl-tin-dimaleate as stabilizer with or without adding dioctyl-phthalate as plasticizer. Conclusions obtained from the study are as follows: The origin of the absorption band at 1,540-1,640$cm^{-1}$ / on I.R. spectrum seems to be RCOO- ion originated from ionization of the stabilizer, and this peak can be useful as a measure of radiation resistance on polyvinylchloride. Addition of increasing plasticizer to polyvinylchloride exhibits increasing radiation resistance and the reason for this result may be attributed to aromatic resonance absorption of radiation energy by diotylphthalate. On dose dependent dielectric characteristics, nonplastized specimen shows a peak at about 10 Mrad and that this peak disappears on the plastification of specimens. Those phenomena may be explainable in considering the statistical distribution of scissored chain molecular segments as well as the plastification process of plasticizer to polyvinylchloride chain molecules.

• 전기학회논문지
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• 제66권3호
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• pp.540-544
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• 2017

In this study, we analyzed the effects of TID(Total Ionizing Dese) and TREE(Transient Radiation Effects on Electronics) on nMOSFET and pMOSFET fabricated by 0.18um CMOS process. The size of nMOSFET and pMOSFET is 100um/1um(W/L). The TID test was conducted up to 1 Mrad(Si) with a gamma-ray(Co-60). During the TID test, the nMOSFET generated leakage current proportional to the applied dose, but that of the pMOSFET was remained in a steady state. The TREE test was conducted at TEST LINAC in Pohang Accelerator Laboratory with a maximum dose-rate of $3.16{\times}10^8rad(si)/s$. In that test nMOESFET generated a large amount of photocurrent at a maximum of $3.16{\times}10^8rad(si)/s$. Whereas, pMOSFETs showed high TREE immunity with a little amount of photocurrent at the same dose rate. Based on the results of this experiment, we will progress the research of the radiation hardening for CMOS unit devices.

• 한국자원식물학회지
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• 제25권6호
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• pp.745-752
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• 2012

Compared to wide ranges of genetic variation of natural populations, very limited Miscanthus cultivar has been released. This study was the first report on the development of Miscanthus cultivar by means of radiation breeding. Seeds of M. sinensis were initially exposed to gamma rays of 250 Gy for 24 h, generated from a $^{60}Co$ gamma-irradiator. The irradiated seeds were sown and then the highly tiller-producing mutants were selected for this study. Biomass-related parameters including tiller number, plant height, stem diameter, and leaf number were measured. Ploidy level and internal transcribed spacer (ITS) were investigated to characterize the mutants compared to wild type (WT) Miscanthus. Plant height and tiller number were negatively related, where multi-tillering mutants were relatively short after 4 month growth. However stem diameter and leaf number were greater in mutants. All the materials used in this study were diploid, implying that the mutants with greater tiller numbers and stem diameter were not likely related to polyploidization. Based on the sequence of ITS regions, the mutants demonstrated base changes from the gamma irradiation where G+C content (%) was decreased in the ITS1, but increased in ITS2 when compared to WT sequence. ITS2 region was more variable than in ITS1 in the mutants, which collectively allows identification of the mutants from WT. Those mutants having enhanced tillers and allelic variations might be used as breeding materials for enhanced biomass-producing Miscanthus cultivars.