• Food Science and Preservation
• /
• v.15 no.1
• /
• pp.118-124
• /
• 2008

The chemical compositions and antioxidant activities of four different colored rice cultivars (white-colored rice: WCR, brown-colored rice: BCR, brown-colored waxy rice: BCWR, black-colored rice: BKCR) were investigated to evaluate the quality characteristics of the new brown rice cultivars. There was no significant differences in chemical compositions amongst the four rice cultivars, although WCR had higher amount of carbohydrate than did the other rice cultivars. 'The major fatty acids in the four rice cultivars were linoleic, oleic and palmitic acids, which accounted for about 96% of total fatty acids. Of note, higher amounts of saturated fatty acids (24.8%) and lower amount of unsaturated fatty acids (75.2%) were observed in BKCR than in the other rice cultivars. Two brown-colored rice cultivars, BCR and BCWR, possessed higher amounts of linoleic acid (40.8% and 42.1%, respectively) than did the other cultivars. The highest level of ${\alpha}$-tocopherol was found in WCR (51.3mg%), followed by BKCR (38.6mg%), BCWR (37.2 mg%), and BCR (34.5mg%). Free amino acid analysis showed that aspartic and glutamic acids were major amino acids of all cultivars, whereas phenylalanine, lysine, and histidine were minor. Particularly, relatively higher contents of aspartic and glutamic acids were found in BKCR, while relatively higher levels of alanine and ${\gamma}$-aminobutyric acid (GABA) were observed in BCR and BCWR. Also, BKCR had the highest phenolic content and antioxidant activity of any cultivar, followed by the two brown-colored rice cultivars and WCR. These results suggested that the new brown rice cultivars may possess quality characteristics intermediate between those of WCR and BCR.

• Korean Journal of Plant Resources
• /
• v.18 no.1
• /
• pp.85-116
• /
• 2005

Gyeryongsan(845m) is located at $36^{\circ}\;18'\;02'\;-36^{\circ}\;23'\;38'$ in latitude, at $127^{\circ}\;11'\;60'-127^{\circ}\;17'\;86'$ in longitude and on Nonsan City and Gongju City in Chungnam Province. Gyeryongsan shows the typical vegetation patterns including the middle area of temperate region represented by the secondary forests of Quercus mongotica and Q. serrata. And we tried to discuss on the distribution and availability of vascular plants including economical plants. The results of plant collection and their investigation from April in 1998 to October in 2002 are as follows : The vascular plants consist of total 684 taxa; 3 hybrids, 12 forms, 84 varieties, 1 subspecies, 584 species, 357 genera, 100 families, 34 orders, 4 classes, 3 subphyla. In this area useful resources plants were 277 taxa$(40.5\%)$ forage source, 261 taxa$(38.2\%)$ edible source, 204 taxa$(29.8\%)$ medicinal source, 80 taxa$(11.7\%)$ ornamental source, 20 taxa$(2.9\%)$ timber source, 3 taxa$(0.4\%)$ industrial raw material source respectively. Also, the Korean endemic plants are 29 taxa($4.2\%$ among total 684 taxa); 5 varieties, 24 species, 27 genera, 20 families. And rare and endangered plants are 2 taxa; Paeonia obovate, Gastrodia elata. Also, the naturalized plants are 31 taxa($4.5\%$ among total 684 taxa and $13.7\%$ among the total naturalized plants in Korea).

• Korean Journal of Food Science and Technology
• /
• v.11 no.4
• /
• pp.242-248
• /
• 1979

To detect proper lytic assay conditions of the crude zumolyase from Arthrobacter luteus, effets of the various factors involved in the lytic system of Sacchromyces sake cultured with shaking in the malt extracts medium were investigated. The results are summarized as follows : 1. The susceptibilities of viable cells of S. sake from logarithmic growth phase to the lytic enzmye were much greater than those of the cells in lag and stationary phases. The cells cultured for 18 hr were the most susceptible to the enzyme. 2. Lytic activity of the enzyme toward the viable cells of S. sake was very low. It was, however, enhanced 4 folds of more by the pretreatment of the cells with 0.05 M sodium sulfite. 3. Lytic activity of the enzyme toward commercial baker's yeast cells was negligible, and the effect of sodium sulfite on the lysis of the cells also was nothing but a little. 4. The lyophilized cells of the baker's yeast showed more susceptibility to the lytic enzyme than viable cells of the yeast. No definite effect of sodium sulfite on the lysis of the lyophilized cells, however, was observed either baker's yeast of S. sake. 5. It appeared that the relationship between the reaction rate and the enzyme concentration on the lysis of the yeast cell walls followed enzyme kinetic theory, but one between the reaction rate and concentration of the yeast cells as substrates showed different pattern from that in enzyme kinetic theory. 6. After the preparation of crude zymolyase was kept at $7^[\circ}C$ for 10 days in the 0.05 M phosphate buffer, pH 7.5, the remainning lytic activity was about 80 %.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.21 no.3
• /
• pp.115-122
• /
• 2001

The purpose of this study was to suggest the possibility of utilizing the wildflower pasture as the livestock herbage sources as well as promoting the public interests. Not only dry matter (DM) yield and soil properties of pastures were observed, but also chemical composition of herbages was analyzed. The experimental design includes four treatments: Conventional pasture(COP, forage 6 species), Bottomgrass pasture(BOP, turf grass 6 species), Native wildflower pasture(NWP, turf grass 6 species + native wildflower 11 species) and Introduced wildflower pasture(IWP, turf grass 6 species + introduced wildflower 9 species). The field trials were carried out on the experimental pasture plots at Chungnam National University throughout from 1997 to 2000. The results obtained are as follows : 1. As wildflower pasture was composed of turf grasses and wildflowers, the yearly mean of DM yield in the wildflower pastures (NWP 6,688kg/ha and IWP 7,240kg/ha) was lower than that of COP(8,592kg/ha) or BOP(7,264kg/ha)(p<0.05). This result indicated that the forage productivity of wildflower pasture for livestock is low. 2. The nutritive quality of herbages from wildflower pasture tended to be slightly low compared to that of COP and BOP. On the other hand, compared with IWP, the content of CP, NDF and lignin of herbages from NWP were lower than those from IWP, while IVDMD was higher(p<0.05). 3. The pH and the content of exchangeable Ca in NWP and IWP soils were tended to be low compared to those of COP in changes of soil properties after 3-years experimental trials, while the contents of organic matter, nitrogen, available phosphate, Mg and K were slightly improved. In conclusion, even though DM yield seem to be lower in wildflower pasture than those of COP, and the nutritive quality of herbages from wildflower pasture tended to be slightly low compared to that of COP and BOP. but, possibility of utilizing herbages from wildflower pasture for livestock was to some extent expected. In addition. compared to COP, wildflower pastures improved the property of soil. Thus, although wildflower pasture was not enough for livestock as a herbage, wildflower pasture has enough possibility for promoting the public interest.

• Journal of Life Science
• /
• v.16 no.4
• /
• pp.571-578
• /
• 2006

HtrA2/Omi, a mitochondrial trypsin-like serine protease, is pivotal in regulating apoptotic cell death. Several lines of recent evidence suggest that HtrA2 is associated with the pathogenesis of neurodegenerative disorders; however, the physiological function of HtrA2 still remains elusive. For studying physiological function of HtrA2 in depth, it is necessary to develop a suitable expression system in the model animal. We therefore utilized the zebrafish as a model animal to establish expression of human HtrA2 (hHtrA2) in vivo. For expression of mature HtrA2 as GFP fusion in zebrafish embryos, the HtrA2 (WT) or (S306A) cDNAs with the C-terminal GFP tag were inserted into the pCS2+ plasmid. Expression patterns of HtrA2 in HEK293 cells were first monitored by immunofluorescence staining and immunoblot assays, showing approximately 64 kDa of the HtrA2-GFP fusion proteins. Subsequently, the hHtrA2 plasmid DNA or in vitro transcribed mRNA was microinjected into zebrafish embryos. The expression patterns of HtrA2 in Zebrafish embryos were monitored by GFP fluorescence in 24 hours-post-fertilization (hpf). Although expression patterns of HtrA2-GFP in developing embryos were different between the injected DNA and mRNA, both nucleic acids revealed good expression levels to further study the physiological role of HtrA2 in vivo. This study provides a suitable condition for expressing hHtrA2 in the zebrafish embryos as well as a method for generating useful system to investigate physiological properties of the specific human genes.

• Journal of Preventive Medicine and Public Health
• /
• v.9 no.1
• /
• pp.55-64
• /
• 1976

This paper presents the results of a survey for the causes of sensori-neural hearing impairment in Korea, The subjects were 1,676 children of total 2,928 enrolled in 16 Deaf Schools; two schools in each area of Seoul, Busan, Kyoungbook, Kyoungnam, Kyounggi and Chunbug, and each one in Chungnam, Chungbug, Chunnam and Jaeju. The data were collected by questionaire with 28 items distributed to their parents. The filling in the check lists were performed by their class teachers, interviewer, for 18 months from September, 1975 to february, 1976. The questionable or missed problems were reaffirmed. The results obtained were as follows. Most of the reasons, 78.5% were acquired characters that could be developed during pregnant period, the time of delivery and the time of after birth. The pure hereditary reasons except the cases complexed with one or two were only 11.3%. Those who could not be defined with any reasons were 10.2%. Among the acquired causes, 5.8% of total subjects were developed for pregnancy: 3.3%, during delivery; and 69.7%, after birth. In the pregnant period, the drug intoxications were 2.4% of total subjects, several diseases such as influenja, bleeding, surgical operation, venereal diseases and rubella etc. were about one percent, and the accompanied with some symptoms of pregnancy intoxication and traumatic events were 2.4%, During time, the cases with delayed rhythmical pain were 16 persons, the immaturities were 11, the asphyxial cases were nine, the errors of forceps delivery were seven, the cases of low body weight inspite of full term were four, the cases with cesarian section were three, the head injuries were two, and the accompanied with three kinds of above reasons were three. During after birth, the cases with acute communicable diseases were 35.4% of total subjects, the fever unknown origin were 16.1%, the chronic otitis media were 3.7%, the meningitis were 3.5%, the gastric and nutritional diseases were 3.5%, the drug intoxications were 4.8%, the blood diseases were 0.3% and the other causes were 2.2%. Here by acute communicable diseases, some importants were measle, 10.1% of total subjects; meningitis, 7.3%; convulsion with some reasons, 4.9%; poliomyelitis. 3.2%; encephalitis, 2.4%; and mumps, rubella, pertusis, scarlet fever, and small pox were somewhat played a role in. Among 59 cases with train diseases, 53 were concussion by the accidents, such as traffic and falling or sliping down etc., the cerebral paralysis and hydrocephalus were two, respectively. And the blood diseases were severe newjaundice in all five cases. If we were summarized with the above mentioned, most of the hearing impairments were introduced by the combined reasons with familial or hereditary factors and the acquired, than by a simple disease. Among the congenital or hereditary hearing impairments classified to now a day, we suppose that the many cases with the acquired causes during pregnancy, delivery and after birth were complexed. Subsequently, the maternal and child health should be more and more developed in our country, also.

• Korean Journal of Microbiology
• /
• v.45 no.4
• /
• pp.332-338
• /
• 2009

A xylan-decomposing bacterium, HY-20, was isolated from the gut of a honeybee, Apis mellifera, and identified as Bacillus sp. The extracellular GH11 xylanase (XylP) gene (687-bp) of strain HY-20 encoded a protein of 228 amino acids with a deduced molecular mass of 25,522 Da and a calculated pI of 9.33. The primary structure of XylP was 97% identical to that of B. pumilus xylanase (GenBank accession no.: AY526092) that has not been characterized yet. The recombinant His-tagged enzyme (rXylP) overexpressed in Escherichia coli BL21 harboring pET-28a(+)/xylP was purified to electrophoretic homogeneity by cation exchange and gel permeation chromatographies. The purified enzyme exhibited the highest catalytic activity toward birchwood xylan at pH 6.5 and $50^{\circ}C$ and retained approximately 50% of its original activity when pre-incubated at $55^{\circ}C$ for 15 min. The recombinant enzyme was completely inactivated by $Hg^{2+}$ (1 mM) and N-bromosuccinimide (5 mM), while its activity was slightly stimulated by approximately 10% in the presence of $Mn^{2+}$ (1 mM), $Fe^{2+}$ (1 mM), and sodium azide (5 mM). rXylP was able to efficiently degrade various polymeric xylose-based substrates but PNP-sugar derivatives and glucose-based polymers were not susceptible to the enzyme.

• KSBB Journal
• /
• v.21 no.3
• /
• pp.204-211
• /
• 2006

For the production of the recombinant human interferon-gamma(rhIFN-${\gamma}$) in Escherichia coli, human glucagon and ferritin heavy chain were used as fusion partners. Even though rhIFN-${\gamma}$ is expressed as an inclusion body form in E. coli because of strong hydrophobicity of itself, over 50% of fused rhIFN-${\gamma}$ was expressed as soluble form in E. coli $Origami^{TM}$(DE3) harboring pT7FH(HE)-IFN-${\gamma}$ which encodes ferritin heavy chain-fused rhIFN-${\gamma}$. In the case of using glucagon-ferritin heavy chain hybrid mutant as a fusion partner, 6X His-tag was additionally introduced to N-terminus of GFHM(HE)-IFN-${\gamma}$ for enhancing purification yields of rhIFN-${\gamma}$. Fusion protein HGFHM(HE)-IFN-${\gamma}$ with two 6X His-tag was more effectively bound to Ni-NTA agarose bead than GFHM(HE)-IFN-${\gamma}$ with a 6X His-tag. rhIFN-${\gamma}$ was completely purified from enterokinase-treated HGFHM(HE)-IFN-${\gamma}$ by Ni-NTA affinity column. For high-level production of rhIFN-${\gamma}$, glucose was used as the sole carbon source with simple exponential feeding rate($2.4{\sim}7.2g/h$) in fed-batch process. The effective lactose concentration for the expression of the rhIFN-${\gamma}$ was $10{\sim}20mM$. Under the fed-batch culture conditions, rhIFN-${\gamma}$ production yield reached 11 g DCW/L for 6 hours after lactose induction.

• Journal of Korean Society of Forest Science
• /
• v.99 no.4
• /
• pp.611-617
• /
• 2010

The current studies aim is to analyze the production cost of walnut tree cultivation and its object was targeted at walnut tree cultivating household region 163. The analysis is as follows. Our domestic walnut tree cultivating households averagely have cultivated about 0.7ha, and planting number per ha was averagely 204, and it showed that compared to the standard planting number (100), the plantation was done close planted. The most cultivar cultivated according to regions were Chungbuk region: sangchon 65.7%, Chungnam region: kwangduk 68.6%, Jeonbuk region: sangchon 98.0%, Gyeongbuk region: daeboo 61.2%. The production cost for cultivating walnuts can be classified into the followings; management cost(4436 thousand won/ha) such as manufacturing cost(292 thousand won/ha), intermediate material cost(3682 thousand won/ha), rent(103 thousand won/ha), employment cost(653 thousand won/ha) etc, and self-serviced expenses such as self-laboring cost(5,834 thousand won/ha), land security cost(490 thousand won/ha), fixed capital cost(834 thousand won/ha), circulating capital cost(234 thousand won/ha) etc. 11,820 thousand won were invested for the production cost of walnut and it made 11,586 thousand won/ha(rate of investment 72.3%) profit, and the net income was 4,196 thousand won/ha(net income rate 26.2%), showing high amount of income. The manufactured walnuts were marketed in Nong-hyup 39.8%, wholesalers 20.8%, dealers 19.8% and recently, as the amount of goods marketed directly to consumers themselves have increased, the income has reached up to 18.9%. At the basis of making most of idle soil, walnut tree's cultivated regions are fairly small, and due to the characteristics of sideline management, it has its limits in searching for production policy locally and promotion strategy of industries. Therefore, if the basic database can be established, subjected only to full-time cultivating households, then not only would the differences between the imported walnuts be reinforced, it would also be possible to transfer into the new and improved distribution system. Furthermore, through establishment of the database, it can be anticipated that it would contribute greatly in the increase of the household income.

• The Journal of Engineering Geology
• /
• v.1 no.1
• /
• pp.54-67
• /
• 1991

In geological media with anisotropic properties, the strength anisotropy in intact rock is the most important issue in engineering aspects. Point-ioad(P/L) strength test designed to estimate the uniaxial compressive strength(${\sigma}_c$) can be used to evaluate the anisotropic strength characteristics of rocks. The relationship between ${\sigma}_c$ and P/L strength indices(I$_s$), obtained from the banded gneisses distributed in the Cheongyang area, varies depend mainly on the dip angle($\beta$) of foliation. The axial P/L strength indicies(I$_{sa}$) decreases with the increment of $\beta$, whereas diametral P/L strength indices(I$_{sa}$) increase with it. However, the ${\sigma}_c$ decreases with the increments of $\beta$ below about 40$^{\circ}$, but it increases with the increments of $\beta$ over about 40$^{\circ}$ in general. The correlation between ${\sigma}_c$ and I$_s$ suggests that ${\sigma}_c$ is related to the Isa withing low angle($\beta$<40$^{\circ}$) and the I$_{sd}$ within high angle ($\beta$>40$^{\circ}$), respectively. The ratios of I$_s$ to ${\sigma}_c$ are obtained as 11 to 14 in the the gneisses in the study area. The ratio of 24, which is generally adopted value, cannot always be truthworth to the gneisses in the study area. The ratio for the dykes, however, show a good correlation as 21 to 24.5 and, the value of 24 can be used for a homogeneous and isotropic materials such as dykes.