• 생약학회지
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• 제52권2호
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• pp.69-76
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• 2021

Cannabis sativa is an important industrial plant utilized to produce fiber, oil, and medicinal ingredients. A chemotype of cannabis is divided into "Drug type" with predominance of tetrahydrocannabinolic acid (THCA) and "Fiber type" with cannabidiolic acid (CBDA). To develop molecular markers for the discrimination of these two types, nucleotide sequences of THCA synthase and CBDA synthase as well as their pseudogenes were retrieved from the recently published cannabis genome in chromosome scale. Gene-specific SNPs were discovered by multiple alignment of these sequences, and 2 dominant marker sets from each gene were designed for selective amplification. Our markers successfully identified "Drug type" and "Fiber type" cannabis plants as well as forensic samples including processed materials. Our molecular markers will provide a fast and efficient system for molecular-based identification of the cannabis plant.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.277-277
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• 2022

Lignin modification has been a breeding target for the improvements of forage digestibility and fermentation in whole crop silage(WCS) rice. In rice, gold hull and internode 2 (gh2) was identified as a lignin-deficient mutant. gh2 exhibits a reddish-brown pigmentation in the hull and the internode is located on the short arm of chromosome 2 and codes for cinnamyl-alcohol dehydrogenase (CAD). To develop WCS rice variety improved digestibility and fermentation, we measured acid detergent fiber (ADF), lignin and total digestible nutrient (TDN) calculated from ADF (TDN=88.9-(0.79% × ADF) and performed marker-assisted selection using CAD(Os2g0187800) gene first intron region specific marker with 55 Jungmo1038/J.collection lines. Those lines had lignin content range from 0.82 to 6.61%, ADF from 15.8 to 45.8%, TDN from 52.7 to 78.8 compared to 'Jungmo1038'(1.53,20.7,72.6), 'J.collection'(0.98,12.8,78.8%) and gh2 were introgressed into 44 lines. Considering on these genotype and low-lignin phenotype, we finally selected 2 elite lines(Suweon668, Suweon669). Suweon668 and Suweon669 line are high biomass-low lignin lines that the ADF content is relatively low, even though the dry matter weight is high. Also they have lodging and shattering resistance and glabrous leaf and hull important to improve cattle palatability. Our results will provide that rice can be improved for forage digestibility and fermentation with low lignin concentration.

• Journal of Animal Science and Technology
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• 제53권1호
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• pp.1-6
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• 2011

연구는 유전체분석에 대해 모의실험한 연구로써 Reference Population (RP)이 구성되었을 때, 표현형 자료가 없고 유전체자료만 있는 Juven 1 또는 Juven 2 세대에 대해 유전평가의 정확도에 대해 알아보고자 연구를 실시하였다. 모의실험의 가정으로 염색체는 1개이며 염색체길이는 100cM로 가정하였다. 초기의 유효집단의 수는 100두의 다형성이 없는 초기집단에서 유전자 효과가 없는 표지인자(Marker)를 0.1cM 및 0.5cM 간격으로 균등하게 단일 염기 돌연변이에 의한 다형성을 발생시켰고 유전자 효과가 있는 QTL 좌위는 Marker와 동수의 비율로 임의위치를 지정하여 돌연변이에 의한 변이성을 생성하였으며 이때 유전자 효과는 Gamma 분포함수(scale=1.66, shape=0.4)에서 생성하였다. 배우자(gamete) 형성과정에서 Haldane의 가정하에 유전자 재조합을 생성하였으며 돌연변이 발생율은 Marker 및 QTL 좌위에서 $2.5{\times}10^{-3}$ 및 $2.5{\times}10^{-5}$의 확률로 발생시켜 1000세대까지 세대번식을 유지하였다. 이 후 1001세대부터 1004세대까지 세대당 2000두의 자손을 생성하였으며 이 때 유전력을 0.1 및 0.5의 가정하에 1001~1002 세대에서 표현형 자료를 생성하였고, 1003~1004세대는 오직 유전체자료만 생성하였다. Bayesian 방법을 이용하여 개체별 육종가를 추정하였으며 표지인자간 거리(0.1cM, 0.5cM), 유전력(0.1, 0.5) 및 반형매 집단크기(20두, 4두)에 따라 참육종가와 추정 육종가간의 상관으로 표현되는 육종가 정확도에 대해 비교한 결과 1003세 대에서 표지인자간 거리가 0.1cM 및 0.5cM일 때 육종가의 정확도는 각각 0.87, 0.81였고, 유전력이 0.1 및 0.5 일 때 각각 0.87, 0.94로 추정되었으며, 반형매 집단의 크기가 20두 일 때 0.87, 4두 일 때 0.84로 추정되었다. 위의 결과로 미루어 보아 다량의 SNP 표지정보 및 반형매 집단의 크기가 클수록 즉, 혈연계수가 높은 집단일 때 육종가의 정확도는 높게 나타났다. 유전체선발의 활용시 비교적 높은 정확도로써 조기선발이 가능하며 이로 인한 세대간격을 단축시킬 수 있어 개량의 효율을 높일 수 있을 것으로 사료된다. 반면에 유전체선발은 분석비용이 비싸며, 지속적인 유전체 선발시 특정유전자 선호로 인한 유전적 부동(Genetic Drift) 현상이 발생될 수 있기 때문에 지속적인 SNP 발굴에 대한 노력이 필요한(Meuwissen 2003) 단점이 있으나 한우 또는 젖소와 같은 대가축과 같이 세대간격이 긴 가축에서 유전체선발 할 경우 조기선발로 인한 세대간격 단축과 유전평가의 높은 정확도(0.8이상)로 인해 개량의 효율을 극대화 할 수 있을 것으로 사료된다.

• 생명과학회지
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• 제25권8호
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• pp.925-931
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• 2015

미립 품질 향상을 위하여 미립 형태를 결정하는 특성을 위한 분자육종기술을 확립하기 위하여 미립과 관련된 양적형질 유전자좌를 탐색하고, 이들 환경요인과 상호작용 효과를 분석한 결과는 다음과 같다. 인디카 품종인 ‘청청’과 자포니카형인 ‘낙동’이 교배된 조합 F₁의 약배양에 의해 양성된 120 계통(DH 집단)과 217개의 DNA 마커를 이용하여 전체 길이가 2,067cM이고, 마커간 평균거리가 9.5cM인 유전자 지도를 작성하였다. 미립형태 관련 유전자좌 분석에서 미립의 외형인 길이, 폭, 두께, 장폭비, 천립중과 관련하여 14개의 QTL이 탐색되었다. 현미의 미립길이 관련 3개의 QTL (qGL2, qGL5, qGL7), 미립 폭 관련 3개의 QTL (qGW2-1, qGW2-2, qGW2-3), 미립 두께 관련 1개의 QTL (qGT2), 장폭비 관련 6개의 QTL (qLWR2-1, qLWR2-2, qLWR2-3, qLWR2-4, qLWR7, qLWR12) 및 천립중 관련 1개의 QTL (qTGW8)이 선발되었다. 미립 장폭비 관련 4개의 QTL은 미립길이와 미립두께에서 동일한 염색체 상에서 확인되었다. 본 연구에서 구명된 QTL 마커들은 쌀 품종개량을 위하여 이용될 수 있을 것이라 판단된다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.529.2-529
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• 1986

pSp-Si (1.6kbp) was originally found in pediococcus halophilus to be a cryptic multicopy-plasmid. Hoping that the plasmid can also replicate in Bacillus subtilis, protoplast transformation of strain 207-25 (recE) was performed using pSP-Sl onto which was added the marker of tmrB8 (on 4.9 kbp EcoRI fragment ) or tmrB+ (on 0.9 kbp xbaI fragment) gene. Though the tmrB8 gene can expres tunicamycin-resistance at the single copy state, and the tmrB+ gene exerts the resistance only at the multicopy state, we could not confirm the replication of pSP-Sl (tmrB8) or pSP-Sl(tmrB+) in B. subtilis. During the experiment, however, we unexpectedly found that the circularized 0.9 kbp xgaI fragment (tmrB+) itself, which had no replication origin, could transform strain 207-25 to tunicamycin-resistant by protoplast transformation. Southern hybridization analyses with tmrB+ and other probes revealed the integration of the fragment at a single copy state into a position other than the homologous tmrB gene. This recE independent integration of another tmrB+ gene into the chromosome may contribute to the tunicamycinresistance in the transformants.

• 생명과학회지
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• 제15권1호
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• pp.45-48
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• 2005

서울대공원에서 사육중인 Miniature horse 10두의 혈통정립을 목적으로 PCR에 의한 성별 판정 및 16개 microsatellite marker를 사용하여 친자감정을 실시하였다. 성별 판정에서 430 bp의 SRY band가 관찰된 7두는 숫말로 판정되었고, 친자감정에서는 멘델의 유전양식에 부합된 3두가 친자관계가 확인되었다. 향후 Miniature horse의 혈통보존 및 관리에 유용한 자료가 될 것으로 사료된다.

• Interdisciplinary Bio Central
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• 제2권4호
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• pp.13.1-13.8
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• 2010

The National BioResource Project of Japan is a governmental project to promote domestic/international research activities using biological resources. The project has 27 biological resources including three cereal resources. The core center and sub-center which historically collected the cereal resources were selected for each cereal program. These resources are categorized into several different types in the project; germplasm, genetic stocks, genome resources and database information. Contents of rice resources are wild species, local varieties in East and Southwest Asia & wild relatives, MNU-induced chemical mutant lines, marker tester lines, chromosome substitution lines and other experimental lines. Contents of wheat resources are wild strains, cultivated strains, experimental lines, rye wild and cultivated strains; EST clones and full-length cDNA clones. Contents of barley resources are cultivar and experimental lines, core collection, EST/cDNA clones, BAC clones, their filters and superpool DNA. Each resource is accessible from the online database to see the contents and information about the resources. Links to the genome information and genomic tools are also important function of each database. The major contents and some examples are presented here.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10585-10589
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• 2015

Breast cancer susceptibility gene 1 (BRCA1), mapped on chromosome 17q21, is implicated in the mechanisms of cellular DNA repair. Inactivation of this gene is involved in the development of many human cancers, including breast cancer. This study aimed to investigate the prognostic value of BRCA1 promoter hypermethylation and expression in breast cancer cases. Sixty-one breast cancers were examined for BRCA1 hypermethylation by methylation-specific polymerase chain reaction (PCR), and 45 paired normal breast tissues were analyzed for altered BRCA1 mRNA levels by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Aberrant methylation status in BRCA1 was detected in 15 of 61 cases (24.6%), while reduced expression was found in 7 of 45 (15.6%). BRCA1 hypermethylation was statistically associated with tumor grade III (p=0.04), a high frequency of stage IIB (p=0.02), and triple-negative phenotype (OR= 3.64, 95%CI =1.1-12.3, p=0.03). Our findings indicated that BRCA1 promoter hypermethylation is a useful prognostic marker for breast cancer.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.169-174
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• 2012

Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.

• Molecules and Cells
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• 제26권3호
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• pp.319-322
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• 2008

Little is known about the chromosomal variability and polymorphism existing in mitotic chromosomes of Citrus, mainly due to lack of reliable chromosomal markers and small chromosome size. To test the hypothesis of chromosomal polymorphism and provide the foundation of the genome organization in the Citrus cultivars, we have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this study will be of utmost importance in cytological systematics and karyotyping of the Citrus species.