• 제목/요약/키워드: Chromosome Number

검색결과 512건 처리시간 0.027초

Development of tetraploid rye (Secale cereale L.) cultivar in Korea

  • Ku, Ja Hwan;Han, Ouk Kyu;Ahn, Jong Woong;Kweon, Soon Jong
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.75-75
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    • 2017
  • Rye is cultivated on about 30,000ha domestically for forage and green-manure production in Korea. 'Daegokgreen' has been developed as a good quality rye cultivar for forage and green-manure, which was doubled-chromosome by colchicine treatment of diploid rye cultivar 'Gogu' in 2010. By the colchicine treatment with 0.05% for 12 hours at $2^{nd}$ leaf stage of 'Gogu', 31 tetraploid plants were obtained and they produced 2,470 seeds with 135 spikes. There was 4.4 in the number of spikes per plant, 18.3 in grain number per spike, and 37.6 g in the1,000-grain weight. The heading date of 'Daegokgreen' was April 11, which was two days later on average compared with 'Gogu'. The biomass (fresh weight) of 'Daegokgreen' was 3,701kg, which was similar to 'Gogu'. The average crude protein content of 'Daegokgreen' was 8.9%, which was 1.0% point higher than 'Gogu'. 'Daegokgreen' was found to be strong resistant to winter-kill and can be adapted to before maize cultivation.

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치료제 DehydroevodiamineㆍHCl(DHED)의 변이원성 연구 (Study on Mutagenicity of DehydroevodiamineㆍHCl(DHED))

  • 성이숙;정성윤;정주연;채규영;진미령;최봉웅;장병모;김대경
    • 약학회지
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    • 제46권3호
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    • pp.208-212
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    • 2002
  • Dehydroevodiamine HCl (DHED), which is a component separated from Evodia rutaecarpa Bentham, has novel anticholinesterase and antiamnesic activities in the scopolamine-induced amnesia model. Several studies suggest that DHED might be an effective drug for the Alzheimer's disease and the vascular type of dementia. In order to evaluate the mutagenic potential of DHED, Salmonella typhimurium reversion assay, chromosomal aberration test on Chinese hamster lung cells, in vivo micronucleus assay using mouse bone marrow cells, and comet assay were performed. DHED did not increase the number of revertant in the reverse mutation test using Salmonella typhimurium TA1535, TA1537, TA98, TA100. DHED HCl, at concentration of 5 and 10 $\mu\textrm{g}$/mι, increased the number of chromosome aberrated Chinese hamster lung cells with 5 and 10%, respectively. In mouse micronucleus test, no significant increase in the occurrence of micronucleated polychromatic erythrocyte was observed in ICR mice orally administered with DHED. DHED was tested for ability to induce genotoxic effect in L5178Y cells (mouse lymphoma cells) using the single cell gel electrophoresis assay (comet assay). In comet assay, tail moment did not increase in L5178Y cells treated with 10, 100, 300 $\mu$M DHED.

Localization of 5S and 25S rRNA Genes on Somatic and Meiotic Chromosomes in Capsicum Species of Chili Pepper

  • Kwon, Jin-Kyung;Kim, Byung-Dong
    • Molecules and Cells
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    • 제27권2호
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    • pp.205-209
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    • 2009
  • The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

Frontotemporal Lobar Degeneration(FTLD)의 임상적, 병리적 특징과 타우 단백질의 분자 유전학 (Clinical and Pathological Characteristics of Frontotemporal Lobar Degeneration(FTLD) and Molecular Genetics of Tau Protein)

  • 우성일
    • 생물정신의학
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    • 제10권2호
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    • pp.97-106
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    • 2003
  • Criticisms about amyloid cascade hypothesis of Alzheimer's disease(AD) are based on the findings, first, that the degree of dementia does not correlate with the number of plaques, and second, that the neurofibrillary tangle formation seems to predate plaque formation. In addition, neurofibrillary tangle counts correlate well with the degree of cognitive impairment. These findings suggest the independent importance of tau abnormality in AD research which is involved in the neurofibrillary tangle formation. Recently, tau pathology without amyloid deposits and mutations in tau protein gene were reported to be the major pathogenic mechanism in Pick's disease, progressive supranuclear palsy, corticobasal degeneration and FTDP-17(frontotemporal dementia and parkinsonism linked with chromosome 17). These data suggest that understanding the causes and consequences of tau dysfunction might give new clinical and therapeutic solutions to many known tauopathies.

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Production of Triploid Somatic Hybrids Between Mandarin and Grapefruit through Electrofusion

  • Kunitake, Hisato
    • Journal of Plant Biotechnology
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    • 제2권2호
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    • pp.109-113
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    • 2000
  • Somatic hybrids were produced by electrofusion between embryogenic callus protoplasts of 'Syougun' mandarin and leaf protoplasts of grapefruit. Hybridity of the two plants was confirmed by leaf morphological characteristics and random amplified polymorphic DNA (RAPD) analysis. The cpDNA analysis using PCR-RFLP could not distinguish those of both parents. These plants showed normal growth and had chromosome number of 27. These unexpected triploid somatic hybrids might be derived from fused cells between diaploid protoplast of embryogenic calli and diploid protoplast of leaf, because polysomaty, a mixture of haploid cells and diploid cells was observed in the lactose medium-pretreated embryogenic calli of 'Syougun' by flow cytomehy analysis.

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유전알고리즘에 기반한 Job Shop 일정계획 기법 (A Genetic Algorithm-based Scheduling Method for Job Shop Scheduling Problem)

  • 박병주;최형림;김현수
    • 경영과학
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    • 제20권1호
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    • pp.51-64
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    • 2003
  • The JSSP (Job Shop Scheduling Problem) Is one of the most general and difficult of all traditional scheduling problems. The goal of this research is to develop an efficient scheduling method based on genetic algorithm to address JSSP. we design scheduling method based on SGA (Single Genetic Algorithm) and PGA (Parallel Genetic Algorithm). In the scheduling method, the representation, which encodes the job number, is made to be always feasible, initial population is generated through integrating representation and G&T algorithm, the new genetic operators and selection method are designed to better transmit the temporal relationships in the chromosome, and island model PGA are proposed. The scheduling method based on genetic algorithm are tested on five standard benchmark JSSPs. The results were compared with other proposed approaches. Compared to traditional genetic algorithm, the proposed approach yields significant improvement at a solution. The superior results indicate the successful Incorporation of generating method of initial population into the genetic operators.

Challenges for QTL Analysis in Crops

  • Long, Yan;Zhang, Chunyu;Meng, Jinling
    • Journal of Crop Science and Biotechnology
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    • 제11권1호
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    • pp.7-12
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    • 2008
  • Quantitative trait loci, a genetic concept for explaining the inheritance of non-Mendelian traits in 1940s, have been realized as particular fragments of chromosome even unique genes in most crops in 21st century. However, only very a small portion of QTL has been screened out by geneticists comparing to a great number of genes underneath the quantitative traits. These identified QTL even have been seldom used into breeding program because crop breeders may not find the QTL in their breeding populations in their field station. Several key points will be proposed to meet the challenges of QTL analysis today: a fine mapping population and the related reference genetic map, QTL evaluation in multiple environments, recognizing real QTL with small genetic effect, map integration.

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배수분조작을 위한 세포유전학적 연구 (Cytogenetic Studies of Polyploidy Manipulation)

  • 손진기
    • 한국가축번식학회지
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    • 제13권1호
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    • pp.49-55
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    • 1989
  • The Results of colchicine and pretreatment time have shown that the cells of Coho Salmon(Oncorhynchus Kisutch) were effectively injected 6 hours prior to the harvest at a final concentration of 10$\mu\textrm{g}$/g body weight. The cell-density from 8,000 cells/㎣ (=8${\times}$106 ml) was found as ideal. The best effect of hypotonic solution in proportion to the cell is 20 : 1 and 50 minutes. The model number of diploid chromosome in this species was 2n=60. The karyotype analysis proved that the diploid complement consisted of 19 pairs of metacentric-, 4 pairs of submetacentric- and 7 paris of acrocentric-chromosomes. The diagrammatic representation proved that the diploid complement consisted of 7 pairs of metacentric-, 2 pairs of submetacentric- and 1 pair of acrocentric-chromosomes.

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디지털 유전자를 사용하는 추상 이미지의 분류 (Classification of Abstract Images using Digital Chromosome)

  • 서동수;이혜리
    • 한국콘텐츠학회:학술대회논문집
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    • 한국콘텐츠학회 2009년도 춘계 종합학술대회 논문집
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    • pp.870-874
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    • 2009
  • 추상 이미지를 자동 생성하는 기법으로 유전자 알고리즘을 이용하는 방법은 다양한 형태의 이미지를 자동 생성할 수 있는 기법으로 유용하게 쓰일 수 있다. 그러나 다수의 자동 생성된 이미지를 보관하고 검색하는 과정에서 적절한 분류 방법이 제공되지 않아 관리의 어려움이 존재한다. 본 연구에서는 아핀 추상 이미지를 효과적으로 분류하기 위한 체계로 형태요소, 감성요소, 색채요소를 이용한 분류 방법을 제안하고 이를 검색할 수 있는 데이터베이스를 설계한다.

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연초포장에서 발생하는 복숭아혹진딧물의 핵형과 살충제 저항성 (Karyotype and Insecticide Resistance of the Green Peach Aphids (Myzus persicae Sulzer) collected from Tobacco fields)

  • 채순용;김영호;김상석;장영덕
    • 한국연초학회지
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    • 제19권2호
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    • pp.107-116
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    • 1997
  • Karyotype of apterous green peach aphid (Myzus persicae SuIBer) populations occurring in tobacco fields and their resistance to insecticides were investigated. All of the 16 clones collected from tobacco fields had same chromosome number of 2n=12. Among them, 5 clones had the normal karyotype, regardless of color morph, but chromosomal translnations between #1 and #3 autosomal chromosomes were noted in the other 11 clones. All of the aphid clones were highly resistant tolambda cyhalothrin, a pyrethroid pesticide, having over 20 times higher concentrations of LD90 than recommended concentration (10ppm) for the aphid. However, their resistance to acephate, demeton 5-methyl and pirimicarh varied depending on the clones, among which green-colored clones were generally more resistant to the pesticides than red-colored ones. No association was found between the insecticide resistance and the karyotype of the aphid in this experiment.

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