• Journal of Korean Neurosurgical Society
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• v.42 no.6
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• pp.470-474
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• 2007

Objective : The brain is dependent on glucose as an energy source. Intricate homeostatic mechanisms have been implicated in maintaining the blood glucose concentration in the brain. The aim of this study is to find the way to identify the metabolic proteins regulating the glucose in rat hypothalamus. Methods : In this study, we analysed the secretome from rat hypothalamus in vivo. We introduced 500 nM of insulin into the rat hypothalamus. The chromatographic patterns of the secretome were identified, after which Mass Spectrometry-Mass Spectrometry (MS-MS) analysis was performed. Results : In Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, 60 proteins were identified in the secretome. Among them, 8 novel proteins were unveiled and were associated with the energy metabolism of insulin signaling in mitochondria of rat hypothalamic neuron. Nineteen other proteins have unknown functions. These ligands were confirmed to be secreting from the rat hypothalmus on insulin signaling by western blotting. Conclusion : The hypothalamus is the master endocrine gland responsible for the regulation of various physiological and metabolic processes. Proteomics using LC-MS analysis offer a efficient means for generating a comprehensive analysis of hypothalamic protein expression by insulin signaling.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.402.3-403
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• 2002

Cilostazol. a quinolinone derivative that inhibits phosphodiesterase. is used for the treatment of intermittent claudication resulting from peripheral arterial disease. In order to perform pharmacological and pharmacokinetic studies of cilostazol, specific. sensitive and reproducible analysis methods are demanded. Therefore. in the present study. an analytical method of cilostazol in human plasma was developed using semi-microbore HPLC equipped with automated column switching system. (omitted)

• Archives of Pharmacal Research
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• v.14 no.2
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• pp.99-102
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• 1991

A hight performance liquid chromatographic methods was developed for the seperation and determination of seven alkaloids in "sanjoin" (the seeds of Zizyphus jujuba Rhamnaceae), a plant with potent sedative activity. A reverse phase system of Lichrosorb RP-Select B column and 0.05 M potassium phosphate buffer (pH = 3.5)-acetonitrile with gradient elution was employed. Two known alkaloids, juzirine and lysicamine, were newly isolated fom "sanjoin"."sanjoin".oin&".ot;.

• Journal of Aquaculture
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• v.9 no.4
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• pp.453-459
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• 1996

Cellulase was purified from marine rotifer, Brachionus plicatilis, to homogeneity by using chromatographic methods. Purified enzyme is an endo-${\beta}$-1,4 glucanase and shows a strong hydrolytic activity against carboxymethyl (CM) -cellulose. The physicochemical parameters of enzyme activity were determined. The molecular weight of the purified protein was approximately 62 kDa as determined by SDS-polyacrylamide gel electrophoresis. The enzymatic capability to digest cellulose of Chlorella cell wall was compared with that of other well known cellulases from Thermomonospora fusca. Experiments involving Chlorella digestion indicated that CM-cellulase from marine rotifer, Brachionus plicatilis, could digest Chlorella very efficiently while cellulase purified from Thermomonospora fusca did not. From the result here, we propose that the cellulolytic system from marine rotifer is responsible for the hydrolysis of cellulosic wall of Chlorella, probing that rotifer digests Chlorella as a major live food.

• Korean Journal of Pharmacognosy
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• v.37 no.3
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• pp.125-129
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• 2006

The chromatographic separation of n-BuOH extract of the aerial parts of Prunus padus (Rosaceae) led to the isolation of two cerebrosides, and six phenolic compounds. Their structure were identified to be pinelloside (1), soyacebroside I (2), $quercetin-3-O-{\beta}-D-galactopyranoside$ (3), nudiposide (4), (+)-isolarisiresinol $9'-O-{\beta}-D-xylopyroanoside$ (5), khaephuoside A (6) and icariside F2(7) by physicochemical and spectroscopic methods. The compounds $1,5{\sim}7$ are first isolated from the genus Prunus.

• Bulletin of the Korean Chemical Society
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• v.35 no.12
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• pp.3465-3474
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• 2014

Porous silica particles are the most prevailing raw material for stationary phases of liquid chromatography. During a long period of time, various methodologies for production of porous silica particles have been proposed, such as crashing and sieving of xerogel, traditional dry or wet process preparation of conventional spherical particles, preparation of hierarchical mesoporous particles by template-mediated pore formation, repeated formation of a thin layer of porous silica upon nonporous silica core (core-shell particles), and formation of specific silica monolith followed by grinding and calcination. Recent developments and applications of useful porous silica particles will be covered in this review. Discussion on sub-$3{\mu}m$ silica particles including nonporous silica particles, carbon or metal oxide clad silica particles, and molecularly imprinted silica particles, will also be included. Next, the individual preparation methods and their feasibilities will be collectively and critically compared and evaluated, being followed by conclusive remarks and future perspectives.

• Archives of Pharmacal Research
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• v.29 no.3
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• pp.203-208
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• 2006

The chromatographic separation of the MeOH extract of the roots of Ainsliaea acerifolia (Compositae) led to the isolation of six known terpenes and two known lignans. Their structures were identified by spectroscopic methods as mokko lactone (1), betulonic acid (2), betulinic acid (3), zaluzanin C (4), $1{\beta}-hydroperoxygermacra-4(15)$, 5, 10(14)-triene (5), pluviatilol (6), (+)-syringaresinol (7), and glucozaluzanin C (8). Compounds $1{\sim}4$ and 8 showed non-specific significant cytotoxicity against five human tumor cell lines with $ED_{50}$ values ranging from $0.36{\sim}5.54{\mu}g/mL$.

• YAKHAK HOEJI
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• v.37 no.3
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• pp.286-289
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• 1993

UV and high performance liquid chromatographic methods for the quantitative analysis of methyl 5-hydroxy-dinaphtho [1,2-2',3'] furan-7,12-dione-6-carboxylate(MHDDC) in urine and blood were developed. The correlation coefficients of the calibration curves of MHDDC in chloroform, methanol and dioxane solution were 0.999, 0.997 and 0.998, respectively. MHDDC was resolved within 15 min and had a detection limit of 2-5ng at S/N=3 by using a reversed-phase column with two solvents (MeOH, HAc).

• Natural Product Sciences
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• v.19 no.3
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• pp.215-220
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• 2013

Three C-glucosyl flavones (1 - 3), one xanthone (4), and four flavanonols (5 - 8) were isolated by various chromatographic methods from the leaves and stems of Desmodium caudatum (Thunb.) DC. Chemical structures of these compounds were elucidated by 1D and 2D NMR and mass spectroscopy. The compounds were identified as swertisin (1), spinosin (2), 7-methyl-apigenin-6-C-${\beta}$-glucopyranosyl-2"-O-${\beta}$-D-xylopyranoside (3), 1,3,5,6-tetrahydroxyxanthone (4), yokovanol (5), aromadendrin (6), 2'-hydroxy yokovanol (7), and 2'-hydroxy neophellamuretin (8). Compounds 2 - 4 were first isolated from D. caudatum, as well as the spectroscopic data for compound 3.

• Journal of Food Hygiene and Safety
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• v.11 no.2
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• pp.149-157
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• 1996

A procedure for the determination of Aflatoxins in food and grains which utilizes reversed phased liquid chromatographic (LC) analysis with postcolumn derivatization by an electrochemical cell and determination with a fluorescence detector has been evaluated. The LC mobile phase was water-acetonitrile-methanol (6+2+2) with 1mM KBr and 1 mM HNO3 which gave baseline separation for the four Aflatoxins (AfB1, AfB2, AfG1, AfG2). The electrochemical cell set at 7V, generated bromine and derivatized aflatoxins B1 and G1, The derivatives were detected by the fluorescence detector. The aflatoxins in naturally contaminated corn samples were isolated by three different cleanup procedures: the AOAC method I column(CB method), a rapid filtrate column (Romer's column), and an immunoaffinity column. The final extract were quantitated with fluordensitometric TLC and the LC postcolumn derivatization techniques. The results were quite similar, however the LC technique showed less interferences and could be automated. Samples of corn, raw peanuts, peanut butter and dried dates were also analyzed successfully with this procedure.