• Title/Summary/Keyword: Chonju

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Isolation of an Autonomously Replicating DNA Sequence from Aspergillus nidulans

  • Jang, Seung-Hwan;Jahng, Kwang-Yeon
    • Journal of Microbiology
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    • v.37 no.2
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    • pp.51-58
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    • 1999
  • Using yeast, Saccharomyces cerevisiae, and the integrate vector system, we have isolated and characterized an autonomously replicating sequence (ARS) from Aspergillus nidulans. The DNA fragment, designated ANR1, is 5.0 kb in size and maintained free from the chromosome in S. cerevisiae. The YIplac211-ANR1 recombinant plasmid, which consists of sequences derived from the yeast integrative vector YIplac211 and 5.0 kb ANR1 fragment, showed a 104-fold enhancement in transformation efficiency over that found for YIplac211, and was easily recovered from the transformed yeast. Genetic analysis of transformants showed that YIplac21-ANR1 could be over 96% cured when cultured over 20 generations in complete medium and thus suggests that this sequence is mitotically unstable. In A. nidulans, recombinant plasmid PILJ16-4.5 which carries the 4.5 kb EcoRI fragment of ANR1 showed a 170-fold enhancement in transformation efficiency compared to that of the integrative vector PILJ16.

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Characteristics of Tobacco Mosaic Virus Isolated from Wasabi (Eutrema wasabi) in Korea

  • Kim, Hyung-Moo;Lee, Kui-Jae
    • The Plant Pathology Journal
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    • v.15 no.4
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    • pp.247-250
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    • 1999
  • Wasabies showing mosaic symptoms were collected and extracted for virus purification. Tobacco mosaic virus (TMV) was identified as causal agent by electron microscopy and nucleic acid and coat protein analyses. TMV strains were determined by enzyme-linked immunosorbent assay (ELISA). TMV was identified as W and C strain in wasabi. The results of host reaction indicated that this virus induced local lesions on Nicotiana tabacum cv. Bright Yellow and N. glutinosa, leaf spots on Chenopodium amaranticolor and mosaic symptoms on wasabi. Rot shape virus particles were observed and was about 300 nm in length. About 6.5 kb single RNA molecule was observed from extracted viral RNA sample and 26 KDa coat protein was detected in denatured acrylamide gel. Infection ratio of TMV was 8% for the first cultivation year, but was 22% for the second year when TMV-W antiserum was used. The results of this experiment showed that infection ratios of both TMV-W and TMV-C strains were higher compared to that of TMV-P strain.

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Primary Students' Conceptions on Atmospheric Pressure (초등학교 학생들의 기압에 대한 개념조사)

  • Chae, Dong-Hyun;Baik, Eun-Mi
    • Journal of The Korean Association For Science Education
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    • v.17 no.3
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    • pp.239-249
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    • 1997
  • Primary students' conceptions on the atmospheric pressure and its related concepts were investigated. Samples consisted of 41 5th grade and 43 6th grade primary school students. Two types of research methods were used: 1) the open-ended written questionnare;2) paper-pencil test. Naive theories on the atmospheric pressure and its related concepts were numerous. For example, 80% students thought that as we go higher into the atmosphere, the atmospheric pressure becomes greater. Also, 50% students thought that the hot air had greater the atmospheric pressure than the cold air. This study was also discussed the teaching strategies to overcome these naive theories on the atmospheric pressure and its related concepts.

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The Flora of Higher Fungi in Mt. Muhack Areas(III) (무학산(舞鶴山) 일대(一帶)의 고등균류(高等菌類)(III))

  • Park, Seong-Sik;Cho, Duck-Hyun;Lee, Ji-Yul
    • The Korean Journal of Mycology
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    • v.15 no.2
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    • pp.71-75
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    • 1987
  • More then 120 species of higher fungi were collected in Mt. Muhack areas during the period from May, 1985 to September, 1986. These higher fungi were identified. As a result of identification, Hygrocybe ovina(Fr.) Kuhn., lnocybe albodisca Peck., I. calospora Que'I., and I. kobayasii Hongo were new to be Korea.

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