• 한국응용곤충학회지
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• 제50권3호
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• pp.215-220
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• 2011

가을재배 배추에서 담배거세미나방 접종시기와 접종밀도에 따라 배추 수량에 미치는 영향을 조사하여 수량감소율에 따른 요방제 수준을 설정하였다. 포장조건에서 담배거세미나방 접종밀도가 다르게 접종하고 3주 동안 배추의 피해엽율을 조사하여 접종밀도와 수량감소율과의 관계식을 산출한 결과, 정식초기(정식 5일 후)는 Y=-21.85X+1300 ($R^2=0.997$)였고, 정식중기(정식 20일 후)는 Y=-12.1X+1382 ($R^2=0.998$)였다. 담배거세미나방 유충밀도와 수량과의 관계식으로 배추 수량 5%를 경감시키는 담배거세미나방 밀도는 정식초기는 2.9마리/100주, 정식중기는 5.6마리/100주 였다.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.773-777
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• 2008

Phosphate solubilizing bacteria (PSB) were isolated from the rhizosphere of Chinese cabbage and screened on the basis of their solubilization of inorganic tricalcium phosphate in liquid cultures. Ten strains that had higher solubilization potential were selected, and they also produced indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and siderophores. The strains were identified to be members of Pseudomonas, by 16S rDNA sequence analysis. Seed bacterization with PSB strains increased the root elongation and biomass of Chinese cabbage in seedling culture, although they had no effect on phosphorus uptake of plants. The plant growth promotion by PSB in this study could be due to the production of phytohormones or mechanisms other than phosphate solubilization, since they had no effect on P nutrition.

• 한국식물병리학회지
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• 제10권3호
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• pp.163-168
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• 1994

Erwinia rhapontici causes soft-rot disease in a number of plants such as rhubarb, onion, hyacinth and garlic. Pectate lyase (Pel) depolymerizes pectin and other polygalacturonates, which is though to play a role in bacterial invasion of plants. Pel activity was not detected in E. rhapontici cultured in a minimal salts medium containing glycerol, polygalacturonate, or citrus pectin as a carbon source. However, when sterilized potato tuber and Chinese cabbage slices were added to minimal salts polygalacturonate (0.5%) medium, E. rhapontici produced pectate lyase enzyme. Also Pel activity was consistently detected from macerated potato tubers, Chinese cabbage leaves, lettuce leaves and celery petioles tissue. Pel in the extract of macerated Chinese cabbage caused by E. rhapontici strain 1, resulted in electrolyte loss, tissue maceration and cell death of potato tuber tissue. These results indicate that E. rhapontici produces pectate lyase only in the presence of non-diffusible plant components, and that this enzyme probably contributes to its pathogenicity.

• 한국환경농학회지
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• 제13권2호
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• pp.209-215
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• 1994

Absorption, translocation, and metabolism of the herbicide alachlor in soybean, Chinese cabbage, and barnyard grass seedlings were examined and compared with each other using [phenyl-U-$^{14}C$] alachlor in search of a primary factor contributing to the selectivity of alachlor. When root of each seedling was immersed into the solution containing [$^{14}C$]alachlor, the amount of absorbed radioactivity/mg dry matter of seedling which was suggested to be correlated with the susceptibility of plants to alachlor decreased in the order of soybean ${\gg}$ Chinese cabbage ${\geq}$ barnyard grass and the rate of translocation to shoot was Chinese cabbage ${\geq}$ barnyard grass ${\gg}$ soybean. These orders did not consistently explain the selective phytotoxicity of alachlor. Analyses of extracts by reverse phase chromatography showed that alachlor was detoxified by conjugation with glutathione in all three plants and the rate of glutathione conjugation of soybean, the resistant species to alachlor, was the greatest, while that of barnyard grass, the susceptible, was the lowest among three plants. This result explained well the selective phytotoxicity of alachlor. Both absorption and translocation contribute undoubtedly to the selectivity by influencing the active internal concentration of alachlor. However, neither of them appeared to be a primary factor. It was concluded that the most important primary factor was the rate of glutathione conjugation, which detoxifies alachlor and plays an important role in selectivity.

• Biomolecules & Therapeutics
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• 제9권3호
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• pp.183-187
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• 2001

Nitric oxide (NO) produced in large amounts by the inducible nitric oxide synthase (iNOS) is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of iNOS, thus, may be useful candidate for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of Chinese medicinal plants and screened their inhibitory activity against NO production in lipopolysaccharide (LPS)-activated macrophages. Among the 80 kinds of extracts of herbal drugs, 15 extracts showed potent inhibitory activity of NO production above 80% at the concentration o$50\mu\textrm{g}/ml$. These potent extracts showed dose dependent inhibition of NO production of LPS-activated macrophages at the concentration of 50, 30,$10\mu\textrm{g}/ml$. Especially, Rhus chinensis, Senecio scandens and Wikstroemia indica showed most potent inhibition above 50% at the concentration of $10\mu\textrm{g}/ml$. These plants are promising candidates for the study of the activity-guided purification of active compounds and would be useful for the treatment of inflammatory diseases and endotoxemia accompanying the overproduction of NO.

• The Plant Pathology Journal
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• 제29권1호
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• pp.105-109
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• 2013

Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high throughput method to detect plants infected by economically important viruses. To enhance the detection sensitivity of Raman measurement, surface enhanced Raman scattering (SERS) was employed. Spectra of extracts from healthy and Turnip yellow mosaic virus (TYMV) infected Chinese cabbage leaves were collected by mixing with gold (Au) nanoparticles. Our result showed that TYMV infected plants could be discriminated from non-infected healthy plants, suggesting the current method described here would be an alternative potential tool to screen virus-infection of plants in fields although it needs more studies to generalize the technique.

• The Plant Pathology Journal
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• 제31권4호
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• pp.363-370
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• 2015

Using the Chinese cabbage (Brassica campestris) cultivar 'Chun-goang' as a host and turnip mosaic virus (TuMV) as a pathogen, we studied the effects of ambient temperature ($13^{\circ}C$, $18^{\circ}C$, $23^{\circ}C$, $28^{\circ}C$ and $33^{\circ}C$) on disease intensity and the speed of systemic infection. The optimal temperature for symptom expression of TuMV was $18-28^{\circ}C$. However, symptoms of viral infection were initiated at $23-28^{\circ}C$ and 6 days post infection (dpi). Plants maintained at $33^{\circ}C$ were systemically infected as early as 6 dpi and remained symptomless until 12 or 22 dpi, depending on growth stage at the time of inoculation. It took 45 days for infection of plants grown at $13^{\circ}C$. Quantitative realtime polymerase chain reaction (q-PCR) results showed that the accumulation of virus coat protein was greater in plants grown at $23-28^{\circ}C$. The speed of systemic infection increased linearly with rising ambient temperature, up to $23^{\circ}C$. The zero-infection temperature was $10.1^{\circ}C$. To study the effects of abruptly elevated temperatures on systemic infection, plants inoculated with TuMV were maintained at $10^{\circ}C$ for 20 d; transferred to a growth chamber at temperatures of $13^{\circ}C$, $18^{\circ}C$, $23^{\circ}C$, $28^{\circ}C$, or $33^{\circ}C$ for 1, 2, or 3 d; and then moved back to $10^{\circ}C$. The numbers of plants infected increased as duration of exposure to higher temperatures and dpi increased.

• Journal of Plant Biotechnology
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• 제3권1호
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• pp.33-38
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• 2001

Protoplasts isolated from inbred lines of Brassica oleracea L. var capitata (cabbage) and Brassica campestris L. ssp. pekinensis (Chinese cabbage) were fused by PEG-mediated method, and somatic hybrid cells were differentiated into plants. for the identification of somatic hybrid plants, ploidy level, plant morphology, and cytological analysis were performed. All of the regenerated plants derived from fused protoplasts were shown to be 2X-4X, or higher ploidy level, presumably due to somatic hybridization or chromosome doubling. The morphology of leaves, petioles, and flowers showed an intermediate phenotype between Chinese cabbage and cabbage. Chromosome numbers in these somatic hybrids ranged mostly from 33 to 38. According to Genomic in situ hybridization (GISH) pattern, signals from both fusion parents of B.campestris or B.oleracea were detected in different colors when chromosomes of putative somatic hybrids were observed.

• Plant Resources
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• 제4권2호
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• pp.85-91
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• 2001

Many plant species are used in China in tradtional medicine for the prevention and treatment of cancer. This paper presents some of these species with defals on other pharmacologic evaluation and its tradional Chinese meditional uses. The known bioaktivities and some chemical constituents of each of the species given. Information on bioactivities of each species resulting from tests on experimental animals are given. Many of the known chemical constituents of each species are given. Various species of plants elective for various types. In this meeting I will present on some antitumor of the plants which are as follows ; (1) Akebia trifoliata(Thunb.)Koidz. ((2) Panax notoginseng (Burk.) F, H, Chen ., (3)Ziziphus jujuba Mill., (4)coriotus versicolor(Fr.) Quel., (5) Trich osanthes kirilowii Maxim., (6) Ficus carica Linnaeus., (7) Acanthopanax senticosus(Rupr. et Maxim.)Harms.,(5) Hibiscus mutabilis Linnaeus.,(9) Arctium lappa Linnaeus., (11) Agrimonia pilose Ledebour and (12) Hedyotis diffusa Willd.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 The 7th International Symposium
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• pp.52-63
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• 2000

Many plant species are used in China in traditional medicine for the prevention and treatment of cancer. This paper presents some of these species with details on other pharmacologic evaluation and its traditional Chinese meditional uses. The known bioaktivities and some chemical constituents of each of the species given. Information on bioactivities of each species resulting from tests on experimental animals are given. Many of the known chemical constituents of each species are given. Various species of plants elective for various types. In this meeting I will present on some antitumor of the plants which are as follows; (1) Akebia trifoliata(Thunb.)Koidz. ((2) Panax notoginseng(Burk.) F, H, Chen., (3) Ziziphus jujuba Mill., (4) coriolus versicolor(Fr.)Que l., (5) Trichosanthes kirilowii Maxim., (6) Ficus carica Linnaeus., (7) Acanthopanax senticosus(Rupr.et Maxim.)Harms., (8) Hibiscus mutabilis Linnaeus., (9) Arctium lappa Linnaeus., (11) Agrimonia pilosa Ledebour and (12) Hedyotis diffusa Willd.