The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Effects of Temperature on Systemic Infection and Symptom Expression of Turnip mosaic virus in Chinese cabbage (Brassica campestris)

  • Chung, Bong Nam (National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Kyung San (National Institute of Horticultural & Herbal Science, RDA) ;
  • Ahn, Jeong Joon (National Institute of Horticultural & Herbal Science, RDA) ;
  • Joa, Jae Ho (National Institute of Horticultural & Herbal Science, RDA) ;
  • Do, Ki Seck (National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Kyo-Sun (National Institute of Horticultural & Herbal Science, RDA)
  • Received : 2015.06.09
  • Accepted : 2015.07.29
  • Published : 2015.12.01
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Abstract

Using the Chinese cabbage (Brassica campestris) cultivar 'Chun-goang' as a host and turnip mosaic virus (TuMV) as a pathogen, we studied the effects of ambient temperature ($13^{\circ}C$, $18^{\circ}C$, $23^{\circ}C$, $28^{\circ}C$ and $33^{\circ}C$) on disease intensity and the speed of systemic infection. The optimal temperature for symptom expression of TuMV was $18-28^{\circ}C$. However, symptoms of viral infection were initiated at $23-28^{\circ}C$ and 6 days post infection (dpi). Plants maintained at $33^{\circ}C$ were systemically infected as early as 6 dpi and remained symptomless until 12 or 22 dpi, depending on growth stage at the time of inoculation. It took 45 days for infection of plants grown at $13^{\circ}C$. Quantitative realtime polymerase chain reaction (q-PCR) results showed that the accumulation of virus coat protein was greater in plants grown at $23-28^{\circ}C$. The speed of systemic infection increased linearly with rising ambient temperature, up to $23^{\circ}C$. The zero-infection temperature was $10.1^{\circ}C$. To study the effects of abruptly elevated temperatures on systemic infection, plants inoculated with TuMV were maintained at $10^{\circ}C$ for 20 d; transferred to a growth chamber at temperatures of $13^{\circ}C$, $18^{\circ}C$, $23^{\circ}C$, $28^{\circ}C$, or $33^{\circ}C$ for 1, 2, or 3 d; and then moved back to $10^{\circ}C$. The numbers of plants infected increased as duration of exposure to higher temperatures and dpi increased.

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