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Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng

  • Sun, Hang;Liu, Fangbing;Sun, Liwei;Liu, Jianzeng;Wang, Manying;Chen, Xuenan;Xu, Xiaohao;Ma, Rui;Feng, Kai;Jiang, Rui
    • Journal of Ginseng Research
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    • v.40 no.2
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    • pp.113-120
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    • 2016
  • Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

The Influence of the Marketing Success of the Musical in the Chinese Musical Industry (뮤지컬 <디에>의 마케팅 성공이 중국 뮤지컬 발전에 끼친 영향)

  • Huang, Biao;Han, Kyung-Hoon
    • The Journal of the Korea Contents Association
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    • v.21 no.1
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    • pp.383-393
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    • 2021
  • This paper analyzed the marketing success factors of the Chinese musical , which entered the world musical market for the first time in the history of Chinese musicals, and investigated the positive effects of the success of on the development of Chinese musicals. First, five important elements of musical marketing were determined as the analysis criteria and through this, we were able to derive that the marketing promotion was achieved by establishing a systematic strategy in the techniques of 'positioning', 'ticket promotion', 'PR strategy', 'producer', 'OSMU' as the success factors of the musical . The case of in Chinese musicals can be regarded as the first successful case by professional marketing. Due to the results of these achievements, the Chinese government and local musical support systems were established, and in recent years, Chinese musicals have been qualitative. It has been confirmed that Chinese musicals are growing rapidly in recent years in terms of quality and industry. It is expected that more academic studies related to Chinese musicals will be conducted through this study, and it is the purpose of this study to be used as a material that can contribute to the creation of Chinese musical with evenly high workability and productability in the future.

Types and Structure of Chinese Companies Outward Foreign Direct Investment (중국기업의 해외직접투자 유형과 구조)

  • Heur, Heung-Ho
    • The Journal of the Korea Contents Association
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    • v.22 no.2
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    • pp.514-528
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    • 2022
  • This study attempted to understand the characteristics of foreign direct investment by Chinese companies from the perspective of Dunning's OLI paradigm. This development of OFDI by Chinese companies was influenced by the Chinese government's policy, internal factors of the Chinese economy, and the economic and institutional environment of the investment target country. The characteristic of Chinese companies' OFDI is that investment in developed countries is gradually increasing amid regional concentration in Asia. And the proportion of tertiary industries is high, In the meantime, the structure of the secondary industry is changing. In addition, Chinese companies are gradually expanding and showing characteristics by considering the economic and political factors of the investment target country in the selection of overseas investment areas, and then selecting areas with little cultural difference from China's system. This characteristic of OFDI by Chinese companies is basically evaluated to be in line with the OLI paradigm of Dunning. However, the difference is that Chinese companies' OFDI not only advances in overseas investment using the strengths of companies, but also advances in investment to compensate for their shortcomings.

Revitalization of Chinese Tourism in Baotu Spring Park of Jinan through Service Design Thinking

  • Feng, SiSi;Yi, Jae Sun;Lee, Ji-Hyun
    • International Journal of Contents
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    • v.15 no.4
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    • pp.50-58
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    • 2019
  • Baotu Spring Park, a must-visit place in Jinan, China, has been rated as the country's highest-level scenic spot by the Chinese government. However, social media that highly reflects public opinions tends to express a lower evaluation of Baotu Spring Park. Thus, this paper employs service design thinking methods to identify the problems of Baotu Spring Park and provide solutions from the perspective of visitors. Based on desk research, tourist behavior tracking, contextual interviews, and a customer journey map, it was found that the service gap of Baotu Spring Park lacks a unified and effective instruction system. Also, the activities, parking, and high-quality tourist souvenirs are deficient, and there is no online information delivery platform. Finally, five suggestions are made,-respectively:,- redesign the sign system, enhance the participation of tourists, redesign the tourist souvenirs and display space, offer parking areas and shuttle buses, as well as set up a dedicated application.

Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng

  • Wei, Guangfei;Yang, Feng;Wei, Fugang;Zhang, Lianjuan;Gao, Ying;Qian, Jun;Chen, Zhongjian;Jia, Zhengwei;Wang, Yong;Su, He;Dong, Linlin;Xu, Jiang;Chen, Shilin
    • Journal of Ginseng Research
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    • v.44 no.6
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    • pp.757-769
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    • 2020
  • Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants. Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles. Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P < 0.05) in the root tissues of P. quinquefolius and P. notoginseng, respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents of Rg1, Re, Rc, Rb2, and Rd in P. quinquefolius. UGT255 was markedly related to the content of R1; CYP74, CYP89, CYP100, CYP103, CYP109, and UGT190 were markedly correlated with the Rd content in P. notoginseng.

Research on the Design of Chinese Online Games in the Application of Cultural Content (문화 콘텐츠를 응용한 중국 온라인(MMORPG)게임 디자인 연구 -돈황벽화를 중심으로-)

The Long Term Effect of Buchu (Chinese chives) Diet on ROS Formation in the Liver and Skin Tissue of ICR mice (장기간의 부추식이가 ICR 마우스의 간과 피부조직의 활성산소종 생성에 미치는 영향)

  • 문갑순;이민자
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.3
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    • pp.444-449
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    • 2003
  • To investigate the long term effect of buchu (Chinese chives) diet on ROS formation in the liver and stin tissue of ICR mice, one of control, 2% or 5% buchu-added diet was fed to ICR mice for 12 months. Superoxide anion (O2ㆍ), hydrogen peroxide($H_2O$$_2$) and hydroxyl radical (ㆍOH) contents were measured in cytosol, microsome, mitochondria of liver and skin of mice, respectively. Behu diet showed a significant decrease of superoxide anion, hydrogen peroxide and hydroxyl radical contents in liver and skin tissues compared to control diet, and this effect is especially higer at 5% than at 2% buchu diet level. ICR mice showed an age-dependent increase in ROS contents, while the group fed buchu diet decreased its ROS contents significantly and ROS contents of liver appeared to be 2 fold higher than skin. The results of the present study suggest that antioxidative components and sulfur-compounds in buchu diet appear to be responsible for the inhibition of ROS formation in ICR mice.

The comparative study of Chinese Design Higher Education before and after the Reform and Opening-up

  • Jiang, Zheng-Qing;Lee, Dong-Hun
    • International Journal of Contents
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    • v.6 no.4
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    • pp.43-48
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    • 2010
  • Because of the social and political reasons, Chinese design higher education has come a long and difficult road. Before and after the Reform and Opening-up, Chinese design higher education system has gone through two different models: "Arts and Crafts" and "Arts & Design". By comparing external environment and Education Concept of two models, this paper points out that the correct direction of design higher education reform is to understand and learn the advantages of two education systems.

Analysis of Sino-American Culture in Disney Animation Mulan

  • ZHEN, ZHAO
    • International Journal of Contents
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    • v.17 no.1
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    • pp.11-17
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    • 2021
  • This article takes Disney animation as the research object to analyze the Chinese and American culture in the animation. The first part introduces the background of the animation. The theme of the animation comes from the long-standing narrative folk song Mulan Ci during the Southern and Northern Dynasties of China, and it introduces the parallel montage of animation narrative. The second part narrates the Chinese cultural elements in Mulan, and expounds on Disney's use and blending of Chinese cultural elements from three aspects of ink painting effect, national costumes and Confucianism. From the perspective of Western culture, the third part analyzes how to integrate Western thought and characteristics, and contrast with Chinese culture in Mulan from three aspects: Mushu, character and hierarchy.

Effect of Young Chinese's Consuming of Korean Cultural Wave Contents on Their Anti-Korean Sentiment: Focusing the Second-order Mediators of Both Liking for Korean Wave and Positive Perceptions of Koreans (한류콘텐츠 이용정도가 중국인의 혐한정서에 미치는 영향 : 한류호감 한국인에 대한 긍정적 인식의 이차매개효과검증을 중심으로)

  • Lee, Hee-Jin
    • The Journal of the Korea Contents Association
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    • v.17 no.10
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    • pp.394-405
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    • 2017
  • The predominant concern of the study consist of: (1) the effect of young Chinese's consuming of Korean cultural wave contents including Korean TV drama, film, and K-pop on their anti-Korean sentiment; (2) the second-order mediating effects of both liking for Korean wave and positive perceptions of Koreans. The research is based on a survey conducted with 695 chinese people between high-teen and 20's, located in eleven cities of seven provinces. The notable findings are as follow: First, the higher consuming of Korean cultural wave contents, the higher anti-Korean sentiment. Second, young Chinese, consuming more Korean cultural contents, show more liking for Korean wave. The more favor of Korean cultural contents, the more positive perceptions of Koreans. The more liking for Koreans wave contests and the more positive perception of Koreans, the less anti-Korean sentiment. Therefore, it is proved that liking for Korean cultural wave contents and perceiving Koreans with a positive view are important mediator to reduce the anti-Korean sentiment. Some practical implications are discussed based on this study's findings above.