• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.4
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• pp.265-270
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• 2003

This study was conducted to determine the influence of seeding date on growth, characteristic yield and chemical composition of Italian millet (Setaria italica) from April 20 to May 30, 2003(April 20, May 1, May 20 and May 30) in Jeju. A local variety was seeded on days to heading were lessened as seeding date was delayed. The plant height was longest when seeded on 1 May(131cm) than at the other seeding dates. Stem diameter, number of leaves, leaf length and leaf width had the similar trend with plant height. SPDA reading values were greater at earlier seedings. Fresh forage yield was greatest (43.28MT/ha) at 1 May seeding and decreased gradually before or after this seeding date. Dry matter yield, crude protein yield and total digestible nutrient (TDN) yield had nearly the same tendency with fresh forage yield. Crude protein, crude fat, NFE and TDN contents were greatest at 30 May seeding and then decreased earlier seedings. Crude ash and crude fiber content tended to decreased as seeding was delayed. Based on the these findings, optimum seeding date for forage production of Italian millet seems to be early May in Jeju island.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.2
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• pp.89-97
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• 1983

This experiment was carried out in order to study the effect of seasonal changes and location differences on the productivity of the native reed (Phragmites Communis Trinius). The samples of reed were taken at about 30-days interval from May to October, 1982, on the open field of the reclaimed land, the river bank and the tideland in Chonnam area, and measured the yield, the feed compositions and in vitro dry-matter digestibility(IVD). The results are summarized as follow: 1. Plant height was rapidly heightened in May and June, and the number of leaves rapidly increased from May to August. However, dry matter yield increased until September and gradually decreased thereafter. 2. In general, the content of crude protein and the in vitro dry matter digestibility decreased with ageing, and the content of fiber increased. 3. Considerable difference by location in the chemical composition including $SiO_2$, Ca and P was observed. 4. There was a significant negative correlation (p<0.05) between the content of crude protein and that of crude fiber. There was a significant positive correlation(p<0.05) between the in vitro dry-matter digestibility(IVD) by two-stage method and the content of crude protein, and also was a significant negative correlation(p<0.05) between the content of fiber and that of crude protein.

• Current Research on Agriculture and Life Sciences
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• v.14
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• pp.85-100
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• 1996

The Studies were conducted to provide the state of mulberry cultivation and it's development in Banguet province (high land) of Philippines. Philippines initiated the sericulture industry with the technical asistance of Japan in 1974 and established mulberry field and sericultural facilities with technical asistance of Korea in 1990 and 1995. The required average of 100 to 150mm per month is not available during the dry season from December to March. Therefore mulching with grass which is available abundantly in the Philippines should be established to conserve moisture, to control soil erosion, surface ran-off and also to increase the humus content in the soil. In chemical properties of mulberry field, the pH value of soil is 4.7, organic matter 1.6%, and available phosphorus 6ppm. Therefore, all fields should do liming and be applied compost. To improve leaf yield for mulberry planted under partial shade area of pine trees, more pruning of pine tree should be done for good sunshining of mulberry, more liming and compost should be applied to improve acidic soil. To control the leaf roller, DDVP and KAFIL are able to be used. When spraying insecticides to control mulberry insect pests, care should be taken to consider the residual effects of chemicals on the leaf. Leaf should be fed to silkworms only after the leaves are free of any residual effects.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.414-423
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• 2016

Growers in plug seedling production think that root media in which rockwool is a component has given rise to several environmental problems. Therefore, the demand for new materials as a substitute for rockwool has been increased. This study examined the possibility of cultivation of tomato plug seedlings using a newly developed growing medium with phenolic foam. Plug seeds of tomato cultivar 'Madison' were sown in four pellet-type growing media: Grodan rockwool (GRW), UR rockwool (URW), phenolic foam LC (LC) or phenolic foam LC-lite (LC-lite). Then, the seedlings were transplanted to the four cube-type growing media 19 days after sowing. Seeds were germinated in a growth chamber ($25{\pm}2^{\circ}C$, 80% relative humidity, and dark) for 4 days and then the seedlings were grown with a nutrient solution supplied by an overhead irrigation system in a greenhouse. Plant height, number of leaves, leaf area, and fresh or dry weight of tomato seedlings were the greatest for the seedlings transplanted to URW cube media after being grown on LC-lite pellets. Root grade was the greatest for the seedlings transplanted on LC or LC-lite cube media after being grown on LC pellets. Chemical properties of all media tested for tomato growth were maintained within a stable range, while physical properties of URW showed high values in container capacity, air space, and total porosity. These results demonstrated that the phenolic foam media were effective for seedling growth and can substitute for rockwool as a root medium.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1137-1143
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• 2011

Optimum soil management of chestnut orchards is important to ensure high quality and yields of chestnut. This study was conducted to evaluate soil and green leaf characteristics by establishment periods of chestnut (Castanea crenata Sieb. et. Zucc.) orchards in Sancheong-gun, Gyeongsangnam-do. Total 30 plots with the criteria of similar cultivation practices were chosen and classified into two establishment periods by over 20-year-old orchards (17 plots) and below 19-year-old orchards (13 plots). Soil bulk density was significantly higher (p<0.05) in over 20-year-old ($1.16g\;cm^{-3}$) than in below 19-year-old ($1.03g\;cm^{-3}$) plots. Soils in over 20-year-old plots were severely acidified with pH 4.56 compared to pH 4.73 in below 19-year-old plots. However, soil organic matter, total nitrogen, available phosphorus, and potassium were not significantly different (p>0.05) between both establishment periods. Leaf area, leaf mass, and nutrient (N, P, K, Ca, Mg) concentration of green leaves were also not significantly different (p>0.05) between both establishment periods. The results indicate that soil bulk density in chestnut orchards is dependent on the establishment periods, while soil chemical property and leaf nutrient concentration may be little influenced by the establishment periods.

• Journal of Life Science
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• v.29 no.6
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• pp.631-636
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• 2019

A new nitric oxide-induced (NOI) gene was isolated by screening ESTs from a cDNA library of dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). The 720 bp cDNA fragment, IbNOI, was sequenced, from which a 77 amino acid residue protein was deduced. A search of the protein BLAST database identified significant similarity to other plant NOI protein sequences. Quantitative RT-PCR analysis revealed diverse expression patterns of IbNOI in various tissues of the intact sweetpotato plant, and in leaves exposed to different stresses. The IbNOI gene was highly expressed in storage roots and suspension-cultured cells. In leaf tissues, IbNOI showed strong expression during sodium nitroprusside (SNP)-induced NO accumulation and chemical stress treatments. Expression of IbNOI was also induced under various abiotic stress conditions, such as dehydration, salt, and bacterial pathogen infection. These results suggest that IbNOI is involved in plant responses to diverse abiotic stresses and pathogen infection through a NO-related pathway.

• Journal of Applied Biological Chemistry
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• v.61 no.4
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• pp.397-403
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• 2018

The leaves of Spiraea prunifolia were extracted with 80% aqueous MeOH and the concentrates were partitioned into EtOAc, n-BuOH, and $H_2O$ fractions. The repeated $SiO_2$ or ODS column, and medium pressure liquid chromatographies for the n-BuOH fraction led to isolation of two phenolic glucosides. The chemical structures of these compounds were determined as isosalicin (1) and crenatin (2) based on spectroscopic analyses including Nuclear magnetic resonance and MS. Extracts were analyzed using UPLC-MS/MS providing a short analysis time within 5 min using MRM technique. The concentration of crenatin was higher as 9.53 mg/g and isosalicin was lower as 0.65 mg/g. Neuroprotective effects of these compounds against hydrogen peroxide ($H_2O_2$)-induced neurotoxicity were evaluated. The results showed that exposure to $H_2O_2$ induced morphological changes, cell death and neurotoxicity in SK-N-MC cells. However, pretreatment with crenatin resulted in inhibition of morphological change, reduction of loss of cell viability and attenuation of neuronal damage. These results suggested that neuroprotective effect of crenatin isolated from S. prunifolia can be a good candidate for the development of health beneficial foods which can ameliorate the degenerative neuronal disease caused by oxidative stress.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.45-45
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• 2019

This experiment was carried out using artificial clay and LED in the plastic film house (irradiation time: 08:00~18:00/day). Seedlings (n = 63 per $3.3m^2$) of ginseng was planted on May 17, 2018. LED was combined with red and blue light in a 3:1 ratio and irradiated with different light intensity. The average air temperature from April to September was $12.3^{\circ}C$ $-26.0^{\circ}C$ and it was the the highest at $26.0^{\circ}C$ in August. The test area where fluorescent lamp was irradiated tended to be somewhat higher than the LED irradiation area. The chemical properties of the test soil are as follows. pH levels was 5.3~5.5, EC levels 0.45~0.52 dS/m and OM levels 33~37%. The total nitrogen content was 0.35~0.47% and the available $P_2O_5$ contents was 13.7~16.0 mg/kg, which was lower than the suitable level of 70~200 mg/kg. Exchangeable cations K and Mg contents were within acceptable ranges, but the Ca contents was $28{\sim}38cmol^+/kg$ levels higher than the permissible level ($2{\sim}6cmol^+/kg$). Germination of ginseng leaves took 8~9 days and the overall germination rate was 70~75%. The photometric characteristics of LED light intensity are as follows. The greater the light intensity, the higher the PAR (Photosynthetic Action Radiation) value, illuminance and solar irradiation. Photosynthetic rate was also increased with higher light intensity was investigated at $1.7{\sim}3.2{\mu}mol\;CO_2/m^2/s$. Leaf temperature ($23.7{\sim}24.8^{\circ}C$) by light intensity was the same trend. The growth of aerial parts (plant height etc.) were generally excellent when irradiated with 3 times the light intensity, the growth of the ginseng aerial parts were excellent as follows. The plant height was 42.6 cm, stem length was 25.2 cm, leaf length was 9.6 cm and stem diameter was 5.0 mm. The growth of underground part (root length etc.) was the same, and the root length was 24.4 cm, the tap root length was 6.0 cm, diameter of taproot was 18.2 mm and the fresh root weight was 17.2 g. There were no disease incidence such as Alternaria blight, Gray mold and Anthracnose. Disease of Damping off occurred 2.2~3.6% and incidence ratio of rusty root ginseng was 14.6~20.7%. Leaf discoloration rate was 13.7~48.9% and increased with increasing light intensity. Ginsenoside content of ginseng by light intensity is under analysis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.46-46
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• 2019

The experiments were performed in the Jinan (elevation: 300 meters above sea level), Jeollabuk-do. Seedlings (n = 63 per $3.3m^2$) of ginseng cultivar (Cheonpung, Yeonpung) were planted on April 10, 2015. Shading material of plastic film house was blue-white film. Before the Planting seedling, silicate (3 kg/10 a) or chitosan (40 kg/10 a) was fertilized and foliar sprayed on the leaves 1000 times dilution solution once a month from May to September every year. The growth results of 5-year old ginseng surveyed in 2018 are as follows. The average air temperature in the plastic film house was the highest at $26.6^{\circ}C$ and $26.5^{\circ}C$ in July and August, respectively, and the highest temperature was $40.5^{\circ}C$ in July. The maximum daily temperature of $35^{\circ}C$ or more was 30 days, with the average soil temperature being $24.9^{\circ}C$ in August. The chemical properties of the test soil are as follows. pH was 6.4~6.9 level and EC was 0.35~0.46 dS/m. The organic matter content was 33.5~41.4 g/kg, and available-P content was 251.9~306.8 mg/kg. Exchangeable cations contents, such as K, Ca and Mg were all the appropriate ranges. The soil microbial density surveyed by the dilution plate method was 10~50 times higher than that of control (Non-treatment) and actinomycete density was 3~6 times higher. Pathogens of the genus Fusarium by Metagenome analysis decreased 91.3% and 68.2% respectively in the foliar sprayed of chitosan and soluble-silicate. The light intensity (PAR) in the blue-white film plastic film house gradually increased until July and then decereased, with the average of light intensity in March-October was $120.3umol/m^2/s$. The growth of aerial parts such as plant height and stem length was better than non-sprayed group in silicate or chitosan treatments and Yeonpung cultivar was superior to the Cheonpung cultivar. The SPAD value was higher in Yeonpung cultivar foliar sprayed with soluble-silicate. The growth of underground parts such as root length and taproot length were better in chitosan and soluble-silicate treatment than control, especially in Yeonpung cultivar foliar sprayed with chitosan was good in taproot length and taproot diameter, and fresh weight of root was 60.1 g. Ginsenoside contents were 24.9 mg/g and 22.4 mg/g, in the Cheonpung cultivar foliar sprayed with soluble-silicate or chitosan respectively, 28% and 15% higher than control (19.5 mg/g). The incidence of disease by Alteraria panax and Botrytis cinerea was 3~9% and 4~9%, respectively. High temperature damage rate was 3~5%.

• Korean Journal of Environmental Agriculture
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• v.39 no.4
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• pp.375-383
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• 2020

BACKGROUND: Bisphenol A (BPA) is a chemical widely used in polycarbonate plastics, epoxy resins. BPA is an endocrine disruptor. Residue of BPA in agricultural environments is a major concern. The objective of this study was to understand the characteristics of the uptake and distribution of BPA and its metabolites introduced into the agricultural environment to crops, and to use it as basic data for further research on reduction of BPA in agricultural products. METHODS AND RESULTS: This study established the analysis method of BPA and its metabolites in soil and crops, and estimated the intake of BPA and its metabolites from lettuce (Lactuca sativa) grown in sandy loam and loam soil, which are representative soils in Korea. The two major metabolites of BPA were 4-hydroxyacetophenone (4-HAP) and 4-hydroxybenzoic acid (4-HBA). BPA, 4-HAP and 4-HBA have been analyzed by using liquid chromatography tandem mass spectrometry (LC-MS/MS). These substances were detected in sandy loam and loam soil, indicating that certain portions of BPA were converted to 4-HAP and 4-HBA in the soil; however, it was observed that only 4-HBA migrated to lettuce through the roots into crops. CONCLUSION: The uptake residues showed the BPA and 4-HAP were not detected in lettuces grown on sandy loam (SL) and loam (L) soil treatments that were applied with of 10 ng/g, 50 ng/kg and 500 ng/g of BPA. However, the 4-HBA was detected at the level of 7 ng/g and 11 ng/g in the lettuce grown in sandy loam and loam soil that were treated with the 500 ng/g of BPA, respectively, while the 8 ng/g of 4-HBA was measured in the lettuce cultivated in the loam that was treated with 100 ng/g of BPA. This result presents that the BPA persisting in the soil of the pot was absorbed through the lettuce roots and then distributed in the lettuce leaves at the converted form of 4-HBA, what is the oxidative metabolite of BPA.