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Isolation and Characterization of a Nitric Oxide-induced Gene in Sweetpotato

고구마에서 질소 유도성 유전자의 분리 및 특성분석

  • Lee, Il Hwan (Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Shim, Donghwan (Department of Forest Bio-resources, National Institute of Forest Science) ;
  • Lee, Kang Lok (Department of Biology Education, IALS, Gyeongsang National University) ;
  • Nam, Ki Jung (Department of Biology Education, IALS, Gyeongsang National University) ;
  • Lee, Shin-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology) ;
  • Kim, Yun-Hee (Department of Biology Education, IALS, Gyeongsang National University)
  • 이일환 (국립산림과학원 산림생명자원연구부) ;
  • 심동환 (국립산림과학원 산림생명자원연구부) ;
  • 이강록 (국립경상대학교 생물교육과(농업생명과학연구원)) ;
  • 남기정 (국립경상대학교 생물교육과(농업생명과학연구원)) ;
  • 이신우 (국립경남과학기술대학교 생명과학대학 농학.한약자원학부) ;
  • 김윤희 (국립경상대학교 생물교육과(농업생명과학연구원))
  • Received : 2019.03.11
  • Accepted : 2019.04.22
  • Published : 2019.06.30

Abstract

A new nitric oxide-induced (NOI) gene was isolated by screening ESTs from a cDNA library of dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas). The 720 bp cDNA fragment, IbNOI, was sequenced, from which a 77 amino acid residue protein was deduced. A search of the protein BLAST database identified significant similarity to other plant NOI protein sequences. Quantitative RT-PCR analysis revealed diverse expression patterns of IbNOI in various tissues of the intact sweetpotato plant, and in leaves exposed to different stresses. The IbNOI gene was highly expressed in storage roots and suspension-cultured cells. In leaf tissues, IbNOI showed strong expression during sodium nitroprusside (SNP)-induced NO accumulation and chemical stress treatments. Expression of IbNOI was also induced under various abiotic stress conditions, such as dehydration, salt, and bacterial pathogen infection. These results suggest that IbNOI is involved in plant responses to diverse abiotic stresses and pathogen infection through a NO-related pathway.

본 연구에서는 산화질소 유도성(nitric oxide-induced, NOI) 유전자를 건조 처리된 고구마 실뿌리의 EST 라이브러리에서 분리하였다. 분리된 IbNOI 유전자의 cDNA는 712 bp의 길이이며, 77개의 아미노산으로 구성되어 있었다. Blast 데이터베이스를 분석한 결과, 식물에서 보고된 NOI 단백질에 속하는 것으로 확인되었다. RT-PCR과 realtime-PCR을 통해 IbNOI 유전자의 발현수준을 고구마 식물체의 조직별로 조사한 결과, 저장뿌리와 현탁배양세포에서 높은 발현 수준을 보였다. 잎에서 산화질소를 유도하는 SNP와 화합물 스트레스들을 처리시, IbNOI의 발현이 증가함을 확인할 수 있었다. 또한 고염, 건조와 같은 비생물학적 스트레스 및 병원균 감염에 의해서도 IbNOI의 발현이 유도됨을 확인할 수 있었다. 본 연구의 결과들을 통해 IbNOI 유전자는 다양한 비생물학적 스트레스 및 병원균 감염 동안 산화질소와 연관된 조절기작을 통해 식물의 방어기능에 관여할 것으로 생각되는 바이다.

Keywords

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Fig. 1. Sequence analysis of the IbNOI full-length cDNA.

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Fig. 2. Expression patterns of IbNOI in various intact tissues of sweetpotato.

SMGHBM_2019_v29n6_631_f0003.png 이미지

Fig. 3. Expression patterns of IbNOI under SNP and chemical stress conditions.

SMGHBM_2019_v29n6_631_f0004.png 이미지

Fig. 4. Expression patterns of IbNOI under different abiotic stress and pathogen infection conditions.

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