• 한국자기공명학회논문지
• /
• 제9권1호
• /
• pp.29-37
• /
• 2005

Intra-cellular drug uptake in Xenopus laevis oocyte has been elucidated using localized MR spectroscopy (MRS) and PFG NMR techniques at a 600 $MH_z$(Bruker, 14.1 T) NMR spectrometer. The localized MRS has been done with a homemade probe, and shows the intra-cellular uptake of nicotinamide. The self-diffusion of the molecule in Xenopus oocyte was obtained by PFG NMR technique. The measured data are well fitted with a linear combination of two exponential functions, which shows that there are two types of drug molecules, intra-and extra-cellular molecules. Diffusion coefficients of intra- and extra-cellular drug molecules are 3.7 $\times$ $10^{-11}$ $\m^{2}/s$and 6.4 $\times$ $10^{-10}$ $\m^{2}/s$, respectively. In the weighting factors there is shown that about 5% of drug molecule is inside the cells. These techniques can be used for drug screening in molecule-, cell-, and tissue-based preclinical test.

• Bulletin of the Korean Chemical Society
• /
• 제32권8호
• /
• pp.2571-2576
• /
• 2011

Surface film formation on $Li(Ni_{1/3}Co_{1/3}Mn_{1/3})O_2$ cathodes upon oxidation of electrolyte during electrochemical cycling was investigated. Information on the important factors for film formation on the cathode can facilitate the design of additives that improve the properties of the cathode. Pyrazole is added to the electrolyte because it is readily oxidized to form a surface film on the cathode. The results of differential scanning calorimetry and Fourier transform infrared spectroscopy (FTIR) showed that the active material played a dominant role in the interfacial film formation with the electrolyte. Carbon black played a negligible role in the surface film formation.

• Korean Chemical Engineering Research
• /
• 제53권1호
• /
• pp.6-10
• /
• 2015

효소 전극 anode와 PEMFC용 전극 cathode를 이용하여 효소연료전지를 구동하였다. 효소 anode는 그래파이트 분말과 효소로서 글루코스 산화제, 전자매개체로서 페로센을 혼합해 압축해서 만들고 Nafion 이오노머로 코팅하였다. anode 제조조건을 변화시키며 OCV를 측정해 효소 anode 제조 최적조건을 찾았다. 효소 anode 압축 시 최적 압력은 9.0 MPa였다. 효소 anode에서 그래파이트가 60%일 때 최고의 OCV를 나타냈다. anode 기질 용액의 최적 글루코스 농도는 1.7 mol/l이었으며, anode의 효소 활성은 7일 동안 안정적으로 유지되었다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVII)
• /
• pp.511-516
• /
• 2005

Sorbitan methacrylate was synthesized from sorbitan dehydrated from D-sorbitol using an immobilized lipase. To optimize the enzymatic synthesis of sorbitan methacrylate, response surface methodology was applied to determine the effects of five-level-four-factors and their reciprocal interactions on sorbitan methacrylate biosynthesis. A total of 30 individual experiments were performed, which were designed to study reaction temperature, reaction time, enzyme amount and substrate molar ratio. A statistical model predicted that the highest conversion yield of sorbitan methacrylate was 100%, at the following optimized reaction conditions: a reaction temperature of 43.06 $^{\circ}C$, a reaction time of 164.25 mins., an enzyme amount of 7.47%, and a substrate molar ratio of 3.98:1. Using these optimal factor values under experimental conditions in four independent replicates, the average conversion yield reached 98.7%${\pm}$1.2% and was well within the value predicted by the model.

• 한국환경과학회지
• /
• 제25권3호
• /
• pp.417-424
• /
• 2016

The objective of this study was to evaluate the microwave drying characteristics of mixtures of chemical wastewater sludge (70~90%) and anthracite coal (10~30%) with respect to physical and economic factors such as mass, volume reduction, moisture content, drying rate and heating value when the wastes were dried at different weight mixing ratio and for different microwave irradiation time. The drying process were carried out in a microwave oven, the combined drying process with a 2,450 MHz frequency and 1 kW of power. Maximum dry rates per unit area on the microwave drying of mixtures with chemical wastewater sludge and anthracite coal were $35.5kg\;H_2O/m^2{\cdot}hr$ for Cs90-Ac10; $40.1kg\;H_2O/m^2{\cdot}hr$ for Cs80-Ac20 and $35.0kg\;H_2O/m^2{\cdot}hr$ for Cs70-Ac30. The result clearly indicated that moisture can be effectively and inexpensively removed from the wastes through use of the microwave drying process.

• The Plant Pathology Journal
• /
• 제24권3호
• /
• pp.245-268
• /
• 2008

The outcome of plant-pathogen interactions is influenced significantly by endogenous small molecules that coordinate plant defence responses. There is currently tremendous scientific and commercial interest in identifying chemicals whose exogenous application activates plant defences and affords protection from pathogen infection. In this review, we provide a survey of compounds known to induce disease resistance in plants, with particular emphasis on how each compound was originally identified, its putative or demonstrated mechanism of defence induction, and the known biological target(s) of each chemical. Larger polymeric structures and peptides/proteins are also discussed in this context. The quest for novel defence-inducing molecules would be aided by the capability for high-throughput analysis of candidate compounds, and we describe some issues associated with the development of these types of screens. Subsequent characterization of hits can be a formidable challenge, especially in terms of identifying chemical targets in plant cells. A variety of powerful molecular tools are available for this characterization, not only to provide insight into methods of plant defence activation, but also to probe fundamental biological processes. Furthermore, these investigations can reveal molecules with significant commercial potential as crop protectants, although a number of factors must be considered for this potential to be realized. By highlighting recent progress in the application of chemical biology techniques for the modulation of plant-pathogen interactions, we provide some perspective on the exciting opportunities for future progress in this field of research.

• 한국해양학회지
• /
• 제6권2호
• /
• pp.63-77
• /
• 1971

An year-long survey of chemical water quality for Lake Eui-am in Kang-won Province, Korea, was conducted from June 1970 to May 1971 to study the water quality and seasonal variations of productivities in relation to selected physical and chemical environmental factors. A monthly series of water samples were taken at the deepest basin of 18m depth of the lake. Water quality parameters determined were water temperature, Secchi disc reading(transparency), pH, O$\_$2/, CO$\_$2/, alkalinity, acidity, Cl, hardness, Ca, Mg, total residue, total ignitious residue, COD, BOD$\_$5/, nutrients, total-Fe, soluble Fe, Mn and Cu. On the whole, the results indicate that the chemical water quality of Lake Eui-am is high, and vary with season. The lake water is characterized that higher levels of dissolved oxygen(8.6 ml/L in mean of whole water) or percent saturation of dissolved oxygen(114%), and of nitrate nitrogen (523 $\mu\textrm{g}$/L). On the other hand, CO$\_$2/(9.6ppm), chlorides(3.5ppm), Ca(7.7ppm) Mg(2.2ppm), hardness(28.5ppm), silica(2.4ppm) and BOD$\_$5/(1.08ppm) are quite low. In terms of nutrient levels, the lake water exhibit slight signs of eutrophication. The high values for nitrate nitrogen, soluble iron and Cu of the lake water suggest that there are some imputs such as domestic and industrial discharges to the lake.

• 한국해양공학회지
• /
• 제24권2호
• /
• pp.41-46
• /
• 2010

This paper deals with the problem of developing a new decision procedure for the freeboard of a coastal chemical tanker going on the coast. The decision procedure is mainly constructed with the algorithm of estimating statistically the time period that deck wetness will last on the deck of the ship. Deck wetness is one of the most important safety factors for sailing of a coaster. It generally means the situation in which the amplitude of the relative motion between the deck and the surface of the wave exceeds the freeboard. Therefore, in this paper, we proposed that the time during which the amplitude remains above the level of the freeboard should be appraised on the basis of statistical theory. A series of numerical calculations were executed for four different coastal chemical tankers (199G/T Type II, III & 499G/T Type II, III). It was demonstrated that the present decision procedure of freeboard is practical for planning the type of coaster sailing in the sea.

• 폴리머
• /
• 제25권1호
• /
• pp.41-48
• /
• 2001

폴리카프로락톤디올(PCD) 및 폴리에틸렌글리콜(PEG), 디이소시아네이트 및 1,2,6-헥산트리올을 용액중합 방법으로 반응시켜 폴리우레탄(PU) 마이크로겔을 합성하였다. PCD/PEG의 몰비가 다른 마이크로겔의 임계 겔화농도의 성질과 생성에 영향을 주는 중요한 인자임을 알았다. 디이소시아네이트, PCD/PEG의 몰비 및 PEG의 분자량을 달리하여 제조한 PU 마이크로겔의 물리적 및 열적 성질을 실험하였다. PU 마이크로겔은 300nm 이하의 다분산성 구형의 작은 입자로 분포되어 있으며, 저점도 특성을 나타내었다.

• 자원환경지질
• /
• 제37권2호
• /
• pp.173-184
• /
• 2004

인도네시아 반둥 남부 수방산지역에 대하여 정밀토양지화학탐사를 실시하였으며, 정밀지표지질조사도 병행하였다. 정밀토양지화학탐사에서 채취한 토양시료의 화학분석 값은 Ag:11ppm(평균함량 : 1.91ppm), Au:548ppb(평균함량 : 42.39 ppb), Cu:89ppm(평균함량 : 51.40 ppm), Pb : 190ppm(평균함량 : 23.69 ppm), Zn: 157ppm(평균함량 ：61.78ppm)이다. 또한 정밀토양지화학탐사를 실시하여 이들에 대한 화학분석치 결과와 통계처리를 한 결과 등 모든 데이터를 종합 분석한 결과, 연구지역 중동부지역에서 다원소 지화학이상대가 확인되었다.