• Journal of Forest and Environmental Science
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• 제36권4호
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• pp.290-297
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• 2020

This study analyzed and compared the damage rate, natural parasitic rate, and the morphological characteristics of Thecodiplosis japonensis larvae, which inhabit forest areas as control areas to large urban areas in 2018 and 2019. This research was conducted to provide basic data for the management of Thecodiplosis japonensis, which harm pine needles, and the results were as follows. First, the gall formation rate of Thecodiplosis japonensis collected from urban areas was upper-crown 35.59% and 34.25%, mid-crown 25.57% and 27.95%, and lower-crown 25.34% and 26.61%; the gall formation rate of Thecodiplosis japonensis was in the order of upper-crown>mid-crown>lower-crown in 2018 and 2019, respectively. In the control areas, the gall formation rates of Thecodiplosis japonensis in mountain villages in 2018 and 2019 were upper-crown 17.72% and 21.78%, mid-crown 13.85% and 16.97%, and lower-crown 15.12% and 15.79%; thus, in the order of upper-crown>lower-crown>mid-crown. The number of larvae in the galls of needles damaged by Thecodiplosis japonensis was as follows: the average number of larvae in the pine trees of urban areas was 9 and 8 in the upper-crown, 7 and 8 in the mid-crown, and 6 and 7 in the lower-crown respectively. This shows that the number of larvae was fewer in the lower-crown than the upper-crown, and that the number of larvae was higher in 2018 than in 2019. For natural parasitic rate of Thecodiplosis japonensis, the gall formation rate and natural parasitic rate of Thecodiplosis japonensis were surveyed; the natural parasitic rate was 12.5% and 11.8% in urban areas while the rate was 21.7% and 20.9% in mountain villages in respectively in 2018 and 2019.

• Parasites, Hosts and Diseases
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• 제58권1호
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• pp.15-25
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• 2020

The third stage larvae (L3) of Anisakis typica were detected in 2 species of threadfin bream, Nemipterus hexodon and N. japonicus, from the Gulf of Thailand, and were morphologically and molecularly characterized. Total 100 threadfin breams, 50 Nemipterus hexodon and 50 N. japonicus, were examined with naked eyes after the opening of abdominal cavity with scissors. Almost all infected larvae remained alive and active even the fish were transported for 1-2 days. Anisakid larvae were exclusively distributed in the body cavity and rarely in the liver. The prevalence of A. typica L3 were 68.0% and 60.0% in N. hexodon and N. japonicus and their infection intensities were 3.5 and 4.2 per fish infected each. Morphological and morphometric analysis were performed by viewing specimens under both a light microscope and a scanning electron microscope. Interestingly, the protruded mucron of Anisakis typica under SEM showed a distinct cylindrical shape that differed from the cone shape of A. simplex. The protruded mucron could be used to identify A. typica L3 larvae in the future. A comparison of the ITS1-5.8S-ITS2 rDNA nucleotide sequences of these species revealed high blast scores with A. typica. Conclusively, it was confirmed that A. typica L3 are prevalent in threadfin breams from the Gulf of Thailand, and their morphological and molecular characters are something different from those of other anisakid larvae, including A. simplex and A. pegreffii.

• Ocean and Polar Research
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• 제32권3호
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• pp.247-254
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• 2010

Single cell PCR analysis and light and scanning electron microscopic techniques were utilized to identify free living bivalve larvae in the coastal waters of Tae-an, on the west coast of Korea. Through DNA sequencing, venerid clam larvae were isolated and identified as Ruditapes philippinarum (99% similarity) and Meretrix lusoria (99%). Under microscopic observation, the D-veliger stage of R. philippinarum exhibited symmetrical shoulder angles and an elliptical ventral form. In contrast, M. lusoria displayed asymmetrical shoulder angles and a round ventral form in the umbonal stage. Size of the R. philippinarum larvae was $156{\pm}22{\mu}m$ in length, $126{\pm}12{\mu}m$ in height, $92{\pm}14{\mu}m$ in width with a length: height ratio of 1.23. Meretrix lusoria was $202{\pm}44{\mu}m$ in length, $161{\pm}35{\mu}m$ in height, $96{\pm}38{\mu}m$ in width with a length: height ratio of 1.25. Experimental results indicate that morphological and molecular characteristics provide evidence for the larval identification of these two venerid clam larvae species in nature.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.369-377
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• 2013

The fertilized eggs of E. septemfasciatus are spherical and transparent with buoyancy at 790 to $890{\mu}m$ (average $821.8{\pm}2.0{\mu}m$) in diameter with 170 to $230{\mu}m$ oil globules (average $192.9{\pm}0.93{\mu}m$). Hatching began approximately 46 and 35 hours after fertilization at $22.0^{\circ}C$ and $25.0^{\circ}C$ water temperature, respectively. The average total length of newly hatched larvae was $1.75{\pm}0.03mm$. Most of the yolk and oil globules were absorbed within 3 to 4 days after hatching. The larvae reached 2.48 to 2.72 mm in total length, and their mouths and anuses opened at 3 to 4 days after hatching. In this time, the mouth diameters of the larvae were 0.209 to 0.238 mm. The larvae reached 3.24 to 4.15 mm in total length at 11 to 17 days after hatching, and began to metamorphose at the time the second dorsal and pelvic spines appeared and elongated. The abdominal cavity was densely lined with melanophores. The larvae reached 5.12 mm in total length at 24 days after hatching.

• 환경생물
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• 제32권1호
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• pp.68-74
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• 2014

본 연구는 서식지 특성에 따른 양서류 유생의 몸상태 변화를 알아보기 위하여 2012년 3월부터 4월까지 한국산 도롱뇽 유생을 대상으로 진행되었다. 우리는 물이 마를 위험이 없는 서식지와 물이 마를 위험이 있는 서식지로 나누어, 유생의 성장과 외부요인에 대한 민감도에 대하여 알아 보았다. 부화 10일 후, 각 유생의 머리에서 눈이 위치한 부분의 넓이(HWE)와 머리에서 제일 넓은 곳의 길이(LHW)와 코끝부터 항문까지의 길이(SVL)를 측정하였으며, 폴리페니즘을 알아 보기 위하여 HWE/LHW의 비율을 사용하여 분석하였다. 물이 마를 위험이 없는 서식지의 유생은 물이 마를 위험이 있는 서식지의 유생보다 큰 SVL를 가지고 태어났으며, 이후의 성장률도 더 빨랐다. 또한 같은 포식자에게 노출되었을 때, 물이 마를 위험이 없는 서식지 유생은 물이 마를 위험이 있는 서식지 유생보다 HWE/LHW가 더 작게 나타났다. 따라서 물이 마를 위험이 있는 서식지 유생은 물이 마를 위험이 없는 서식지 유생보다 외부요인에 대하여 더 민감하게 반응하는 것을 알 수 있었다.

• 한국응용곤충학회지
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• 제44권2호
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• pp.139-144
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• 2005

우리나라에서 약용으로 가장 많이 활용되고 있는 흰점박이꽃무지의 형태와 생육특성에 관하여 조사하였다. 성충은 야외에서 7월 상순 하순부터 출현하였으며 출현빈도가 가장 높은 시기는 8월 상순이었다. 또한 소수의 개체는 성충으로 월동하였다. 유충은 부엽토속에서 서식하며 11월까지는 모두 3령이 되어 월동하였다. 실내에서 암컷 1마리당 평균 152개를 산란하였고, $25^{\circ}C$와 $30^{\circ}C$에서 사육한 결과 알, 유충, 번데기 기간은 모두 높은 온도에서 발육기간이 짧았다. 성충수명은 암컷이 수컷보다 길었고, 집단사육 시보다는 개별사육 시 길었고, 높은 온도보다는 낮은 온도에서 길었다.

• 한국응용곤충학회지
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• 제44권3호
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• pp.207-212
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• 2005

우리나라에서 서식하는 풍뎅이류 중에서 가장 큰 장수풍뎅이의 형태와 생육특성에 관하여 조사하였다. 성충은 야외에서 6월 하순부터 8월 하순까지 출현하며, 출현빈도가 가장 높은 시기는 7월 중순이고, 1년에 1회 발생하였다. 유충은 부엽토속에서 서식하며, 10월 하순까지는 모두 3령이 되어 월동하였다. 실내에서 암컷 1마리당 평균 24개를 산란하였고, $25^{\circ}C$와 $30^{\circ}C$에서 사육한 결과 알, 유충, 번데기 기간은 모두 높은 온도에서 짧았다. 성충의 수명은 암컷이, 집단사육 시 보다는 개별사육시에, 생육온도는 낮은 온도에서 더 길었다.

• 한국수산과학회지
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• 제49권4호
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• pp.486-492
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• 2016

This study investigates the relationship between environmental factors, such as semilunar rhythm and atmospheric pressure, and the release of Marphysa sanguinea larvae. During artificial seed production in a temperature-regulated culture system, there was an increase in the number of released larvae at tide times between 3-4 and 9-11. Numbers of larvae released were significantly correlated with tidal rhythms. Atmospheric pressure also appeared to influence larval release, with increased numbers released when a period of high atmospheric pressure followed a period of low pressure.

• 한국축산식품학회지
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• 제43권6호
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• pp.1031-1043
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• 2023

The purpose of this study was to investigate the functional properties of salt-soluble proteins obtained from Protaetia brevitarsis (PB) and Tenebrio molitor (TM) larvae, the interaction between these proteins and pork myofibrillar protein (MP) in a gel system. The gel properties of salt-soluble protein extracts showed that the PB had a higher viscosity than the TM protein. However, the TM protein had higher gel strength compared with the PB protein. The gelation characteristics of the pork MP gel systems added with lyophilized insect salt-soluble protein powder showed to decrease slightly viscosity compared with MP alone. Adding the TM or PB protein powder did not affect the pork MP's hydrophobicity and sulfhydryl group levels. Furthermore, the protein bands of the MP did not change with the type or amount of insect salt-soluble protein. The cooking yields of the pork MP gels containing PB or TM protein powder were higher than those without insect protein. Regardless of the type of insect salt-soluble protein added, the pork MP's gel strength decreased. Furthermore, as the level of insect powder increased, the surface protein structure became rough and porous. The results demonstrated that proteins extracted from PB and TM larvae interfered with the gelation of pork MP in a gel system.

• Parasites, Hosts and Diseases
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• 제48권2호
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• pp.139-143
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• 2010

Reptiles were known to serve as paratenic hosts for Centrorhynchus (Acanthocephala: Centrorhynchidae) in Korea, but the infection course in experimental animals was not elucidated yet. In this study, the tiger keelback snakes (Rhabdophis tigrinus) were collected and digested with artificial pepsin solution, and the larvae of Centrorhynchus were recovered from them. Then, the collected larvae were orally infected to rats for developmental observations. In rats, all the larvae were observed outside the intestine on day 3 post-infection (PI), including the mesentery and abdominal muscles. As for the development in rats, the ovary of Centrorhynchus sp. was observed at day 15 PI, and the cement glands were 3 in number. Based on the morphological characteristics, including the arrangement of proboscis hooks, these larvae proved to be a species of Centrorhynchus, and more studies were needed for species identification.