Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Identification of Ruditapes philippinarum and Meretrix lusoria Larvae Using Single Cell PCR Analysis and Microscopic Observation

Single Cell PCR과 현미경을 통한 바지락 및 백합 유생의 동정

  • Jung, Seung-Won (South Sea Environment Research Department, KORDI) ;
  • Kim, Chang-Soo (Korea Institute of Coastal Ecology, Inc.) ;
  • Yoo, Jae-Won (Korea Institute of Coastal Ecology, Inc.) ;
  • Kim, Young-Ok (South Sea Environment Research Department, KORDI) ;
  • Lee, Jin-Hwan (Department of Green Life Science College of Natural Sciences Sangmyung University) ;
  • Hong, Jae-Sang (Department of Oceanography, College of Natural Sciences Inha University)
  • 정승원 (한국해양연구원 남해특성연구부) ;
  • 김창수 (한국연안환경생태연구소) ;
  • 유재원 (한국연안환경생태연구소) ;
  • 김영옥 (한국해양연구원 남해특성연구부) ;
  • 이진환 (상명대학교 자연과학대학 그린생명과학과) ;
  • 홍재상 (인하대학교 자연과학대학 해양과학과)
  • Received : 2010.05.07
  • Accepted : 2010.08.28
  • Published : 2010.09.30
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Abstract

Single cell PCR analysis and light and scanning electron microscopic techniques were utilized to identify free living bivalve larvae in the coastal waters of Tae-an, on the west coast of Korea. Through DNA sequencing, venerid clam larvae were isolated and identified as Ruditapes philippinarum (99% similarity) and Meretrix lusoria (99%). Under microscopic observation, the D-veliger stage of R. philippinarum exhibited symmetrical shoulder angles and an elliptical ventral form. In contrast, M. lusoria displayed asymmetrical shoulder angles and a round ventral form in the umbonal stage. Size of the R. philippinarum larvae was $156{\pm}22{\mu}m$ in length, $126{\pm}12{\mu}m$ in height, $92{\pm}14{\mu}m$ in width with a length: height ratio of 1.23. Meretrix lusoria was $202{\pm}44{\mu}m$ in length, $161{\pm}35{\mu}m$ in height, $96{\pm}38{\mu}m$ in width with a length: height ratio of 1.25. Experimental results indicate that morphological and molecular characteristics provide evidence for the larval identification of these two venerid clam larvae species in nature.

Keywords

References

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