• KSBB Journal
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• v.15 no.5
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• pp.434-437
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• 2000

The contents and composition of alkaloids homoharringtonine, harringtonine, and cephalotaxine were determined in Korean native plumyew tree (Cephalotaxus koreana) collected from 17 different sites in Korea. The alkaloid contents of C. koreana varied with locations and plant populations from 11.8 mg/g to 195.2 mg/g of dry weight while C. koreana in southern area had higher alkaloid contents. The alkaloid compositions in C. koreana were highest with homoharringtonine (40%) and followed harringtonine (32%) and cephalotaxine (28%). Total alkaloids and homoharringtonine contents in needles were higher than those of stem by 2 fold. Alkaloid contents of C. koreana were the highest in winter, and the lowest in summer. Comparison of alkaloid contents and composition with other species of Cephalotaxus revealed that C. koreana was different to C. harringtonia, and contents of homoharringtonine were higher than other species. C. koreana could be potential source for homoharringtonine and related alkaloids.

• Natural Product Sciences
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• v.6 no.1
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• pp.1-4
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• 2000

Four biflavonoids were isolated from the leaves of Cephalotaxus koreana. Based on spectroscopic data, the compounds were identified as amentoflavone-7',7",4',4"'-tetramethyl ether, sciadopitysin, ginkgetin and bilobetin, respectively.

• Korean Journal of Plant Resources
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• v.13 no.2
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• pp.89-94
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• 2000

Cephalotaxus koreana Nakai is endemic species of Korea. Since old days, the fruits have been used as a parasiticide and leaves have been used for a pierced wound by insects. The essential oil components from its aerial parts and stem bark by steam distillation were investigated. Several compounds were characterized by GC-Mass spectra. It was found that the leaves, stembark and flowers contain the compounds of essential oils, 1-octene-3-ol, hexadecanoic acid; $\alpha$-pinene, $\Delta^3$-carene mainly, linalylacetate, $\beta$-cubebene, 3,4-octadine-7-methyl , ferruginol(stem bark) and $\alpha$-pinene mainly, $\beta$-pinene , cyclopropane-1,1-dimethyl-2-(3-methyl-1,3-butadienyl), etc. This Cephalotaxus spp. contains the first components of ferruginol(M.W.286.03, $C_{20}H_{30}O)$ which belong to diterpene.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.495-496
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• 2010

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.566-569
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• 2005

A novel purification method was developed for producing homoharringtonine from Cephalotaxus koreana, to guarantee high purity and yield. Our simple, efficient procedure for isolating and purifying homoharringtonine from C. koreanabiomass consisted of solvent extraction, synthetic adsorbent treatment, low-pressure chromatography, followed by high performance liquid chromatography (HPLC). The use of active clay treatment and silica gel low-pressure chromatography in the pre-purification process allowed for the rapid, efficient separation of homoharringtonine from interfering compounds and dramatically increased the yield and purity of crude homoharringtonine for high-performance liquid chromatography (HPLC) purification steps compared with alternative processes. Homoharringtonine could be obtained simply with high yield and purity from biomass using this purification method, while minimizing solvent use and the scale and complexity of HPLC operations for homoharringtonine purification. Purified homoharringtonine was identified and characterized.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.521-524
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• 2003

An effective purification method was developed for producing Homoharringtonine (HHT), to guarantee high purity and yield from Cephalotaxus koreana. This process was a simple and efficient procedure, for the isolation and purification of HHT form the biomass of Cephalotaxus koreana, consisting of extract, adsorbent treatment, precipitation and followed by a chromatography. The extraction, adsorbent treatment and precipitation in pre-purification process allows for rapid and efficient separation of HHT from many compound and dramatically increases the yield and purity of crude HHT for HPLC purification steps compared to alternative processes. This purification processes serves to minimize solvent usage, size, and complexity of the operations for HHT purification.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.351-354
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• 2005

A new isolation and purification method was developed aiming at increasing yield and purity for homoharringtonine. This method was a simple andefficient procedures, for the isolation and purification of homoharringtonine from Cephalotaxus koreana, consisting of solvent extraction, adsorbent treatment, low-pressure chromatography, and high performance liquid chromatography (HPLC). The crude homoharringtonine was efficiently pre-purified adequately to perform HPLC through a combination withadsorbent treatment and low-pressure chromatogaphy. The homoharringtonine can be simply obtained with high purity and yield from crude homoharringtonine by HPLC. Purified homoharringtonine was characterized.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.200.1-200.1
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• 2003

Cephalotaxus koreana Nakai is an endemic species in Korea. The EtOH extract of leaf and branch from the plant showed potent antitumor activity in Teruhiro's method. The tumor volume inhibition ratio value is 25.2% with 20mg/kg in the BDF1 mouse injected LLC cell. We isolated one flavone, sciadopitysin (1), two flavone O-glycosides, quercetin 3-O-${\beta}$-D-glucuronide 6"-ethyl ester (2), apigenin 7-neohesperidoside (3) in comparison with literatures data. Compounds 1-3 showed stronger antitumor activity than Taxol used as positive control. The inhibition ratio values of compounds 1-3 is 34.9, 31.6, 34.0%, respectively, and Taxol is 27.0 % compared with control group.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.491-495
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• 2009

This study was conducted to investigate the propagation characteristics of Cephalotaxus koreana by cutting, whose plant has been known to new anticancer treatments. The treatments of plant growth regulators, cutting media temperature and growing media were investigated to know the rooting ability of cuttings. For rooting formation affected by plant growth regulators and its concentration, The application of IBA 100 mg/L was most effective in all of the characteristics including rooting rate of 88.5%. The optimum media temperature by cutting was $24^{\circ}C$ (treatment IBA 100 mg/L, rooting rate 84.6%), predicting that greenwood cutting from June to July is most effective. VPPL (vermiculite+peatmoss+perlite 1:1:1(v/v/v)) showed the highest value in all of the characteristics including rooting rate of 68.5% for the effect of growing media on rooting formation.

• Journal of Korean Society of Forest Science
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• v.94 no.6
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• pp.397-401
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• 2005

Homoharringtonine isolated from the plumyew tree (Cephalotaxus koreana) is currently considered as one of the most promising chemotherapeutic agents for anti-cancer. Variation of homoharringonine contents from eight natural populations of Korean native plumyew trees was determined by HPLC (high performance liquid chromatography), and compared with the variation among populations, the variation of the different parts(needle, root, stem and seed), and the variation according to their ages and growth features. Homoharringonine contents among populations were significantly different with Mt. South Dukyu ($1,048{\mu}g/g$) having the highest and Mt. Obong having the lowest. The analyses of plant pans showed that the homoharringtonine contents were highest in plant needles ($874{\mu}g/g$), and followed by roots, stems and seeds. Homoharringtonine contents of the plumyew trees decreased about 30% in increment with their ages and growth.