Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Variation of Homoharringtonine Contents from Eight Natural Populations of Korean Native Plumyew Trees(Cephalotaxus koreana)

한국산(韓國産) 개비자나무(Cephalotaxus koreana) 8개 천연집단(天然集團)의 Homoharringtonine 함량변이(含量變異)

  • Jung, Myung-Suk (Department of Forest Science, Seoul National University) ;
  • Hyun, Jung-Oh (Department of Forest Science, Seoul National University) ;
  • Lee, Wi-Young (Korea Forest Research Institute) ;
  • Lee, Ho-Jae (Department of Forest Science, Seoul National University) ;
  • Kosal, Sal (Department of Forest Science, Seoul National University)
  • Received : 2005.06.22
  • Accepted : 2005.10.10
  • Published : 2005.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Homoharringtonine isolated from the plumyew tree (Cephalotaxus koreana) is currently considered as one of the most promising chemotherapeutic agents for anti-cancer. Variation of homoharringonine contents from eight natural populations of Korean native plumyew trees was determined by HPLC (high performance liquid chromatography), and compared with the variation among populations, the variation of the different parts(needle, root, stem and seed), and the variation according to their ages and growth features. Homoharringonine contents among populations were significantly different with Mt. South Dukyu ($1,048{\mu}g/g$) having the highest and Mt. Obong having the lowest. The analyses of plant pans showed that the homoharringtonine contents were highest in plant needles ($874{\mu}g/g$), and followed by roots, stems and seeds. Homoharringtonine contents of the plumyew trees decreased about 30% in increment with their ages and growth.

개비자나무로부터 추출한 HHT(homoharringonine)는 현재 가능성 있는 암화학요법제로 주목받고 있다. 국내 개비자나무 8개 천연집단의 HHT 함량 변이는 HPLC(high performance liquid chromatography)를 이용하여 조사하였고, 집단간 변이와 개체내의 부위별(잎, 줄기, 뿌리 및 종자) 변이 및 수령과 생장특성에 따른 함량 변이를 비교 분석하였다. 개비자나무의 집단간 HHT 함량 변이는 집단간에 통계적으로 유의한 차이가 인정되었으며, 거창의 남덕 유산 집단이 $1,048{\mu}g/g$으로 가장 높았고 완도의 오봉산 집단이 가장 낮았다. 개체내 부위별 함량은 잎에서 $874{\mu}g/g$으로 가장 높았으며, 다음으로 뿌리, 줄기, 종자 순으로 나타났다. 개비자나무의 HHT 함량은 수령이 증가하고 생장이 왕성해 질수록 대략 30% 정도 감소하는 것으로 나타났다.

Keywords

References

  1. 김상익. 최형균. 송재영. 김진현. 이현수. 홍승서. 2000. 한국산 개비자나무 (Cephalotaxus koreana)의 알칼로이드 함량분석 : 지역 및 계절에 따른 변화, 한국생물공학회지 15: 434-437
  2. 박호일. 이연. 이현채. 윤차원. 이계숙. 권기락. 이인. 신동수. 주우홍. 1996 한국산 개비자(Cephalotaxus koreana)에서의 Harringtonine과 Homoharringtonine의 확인 및 함량 분석. 한국생물공학회지 11: 689-695
  3. 이진우. 박종희. 김혜경. 이정규. 2004. 세잎돌쩌기의 알칼로이드 성분과 함량의 계절적 변화. 한국생약회지 35: 128-133
  4. 이창복. 1980. 한국수목도감. 향문사. 서울. pp. 58
  5. Berenbaum, M.R., A.R. Zangerl and J.K. Nitao. 1986. Constraints on chemical coevolution: wild parsnips and the parsnip webworm. Evolution 40: 1215-]228 https://doi.org/10.2307/2408949
  6. Dudt, J.F. and D.J. Shure. 1994. The influence of light and nutrients on foliar phenolics and insect hervivory. Ecology 75: 86-98 https://doi.org/10.2307/1939385
  7. Fu, L., N. Li and R.M. Robert. 1999. Pinaceae. In Wu Zheng-yi and Peter H. Raven (eds.). Flora of China, 4. Beijing: Science Press; St. Louis: Missouri Botanical Garden, http://www.botanik.uni-bonn.de/conifers/refs/fu99.htm (2004. 8. 10)
  8. Huang, M. T. 1976. Drug-mediated increase of tumor immunogenicity in vivo for a new approach to experimental cancer immunotherapy. Molecular Pharmacology 11: 511-519
  9. Jingyi, H., A.P. Cheung, E. Wang, E. Struble, K. Fang, N. Nguyen and P. Liu, 2000. Stability-indicating LC assay and impurity identification in homoharringtonine samples. Journal of Pharmaceutical Biomedical Analysis 22: 541-554 https://doi.org/10.1016/S0731-7085(99)00314-3
  10. Miah, M.A., J.T. Hudlicky and J.W. Reed. 1998. Cephalotaxus alkaloids, In Alkaloids. Academic Press. New York. 51: 199-265
  11. Ohnamam, T. and J.F. Holland. 1985. Homoharringtonine as a new antileukemic agent. Jounal of Clinical Oncology 3: 604-606
  12. Powell, R.G., D. Weisleder and C.R. Smith. 1972. Antitumor alkaloids from Cephalotaxus harringtonia: structure and activity. Journal of Pharmaceutical Sciences 61: 1227-1230 https://doi.org/10.1002/jps.2600610812
  13. Phillips, R. and G.G. Henshaw. 1977. The regulation of synthesis of phenolics in stationary phase cell cultures of Acer pseudoplatanus. Journal of Experimental Botany 28: 785-794 https://doi.org/10.1093/jxb/28.4.785
  14. Rao, K.V., R.S. Bhakuni, J. Juchum and R.M. Davies. 1996. Taxanes from the bark of Taxus brevifolia. 1995. Phytochemistry 41: 863-866 https://doi.org/10.1016/0031-9422(95)00672-9
  15. van Gelderen, D.M. 1986. Conifers. Helm, London. pp. 107
  16. Visani, G., D. Russo, E. Ottaviani, P. Tosi, D. Damiani, A. Michelutti, S. Manfroi, M. Baccarani and S. Tura. 1997. Effects of homoharringtonine alone and in combination with alpha interferon and cytosine arabinoside on 'in vitro' growth and induction of apoptosis in chronic myeloid leukemia and normal hematopoietic progenitors. Leukemia 11: 624-628 https://doi.org/10.1038/sj.leu.2400608
  17. Walsh, V. and G. Jordan. 1999. Cancer chemotherapy, biodiversity, public and private property: the case of the anti-cancer drug Taxol. Social Science and Medicine 49: 1215-1225 https://doi.org/10.1016/S0277-9536(99)00161-6
  18. Wang, D.Z., G.E. Ma and R.S. Xu. 1992. Studies on the alkaloids of cephalotaxus. IX. semi-synthesis of cephalo-taxine esters and their anti-leukemic activity. Yaoxue Xuebao 27: 178-184
  19. Wickremesinhe, E.R.M. and R.N. Arteca. 1996. HPLC separation of cephalotaxine, harringtonine and homoharringtonine from callus and root cultures of Cephalotaxus harringtonia. Journal of Liquid Chromatography and Related Technologies 19: 889-897 https://doi.org/10.1080/10826079608001919
  20. Zhou, D.C., R. Zittoun and J.P. Marie. 1995. Homoharringtonine: an effective new natural product in cancer chemotherapy. Bull Cancer 82: 987-995