• Title/Summary/Keyword: Cell retention

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Preparation of Cationic Chitosan and Its Application as a Multifunctional Chemical Additive

  • Zhong, Liu;Sang, Yi-Zhou
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.06b
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    • pp.401-411
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    • 2006
  • The cationic chitosan is prepared in this paper. This kind of cationic chitosan is a good retention aid for bleached hardwood pulp, the filler retention increased by 33.0% when the cationic chitosan (DS=1.27) dosage was 0.05%. Because many of the materials used in papermaking process are excellent microbiological nutrients, these nutrients will result in the growth of bacteria; uncontrolled growth of bacteria and fungi in the papermaking process adversely affects machine runnability. According to the standard methods of microbe growth inhibition test, cell counting was conducted after proper cultivated time. This paper explored the factors that affecting the cationic chitosan's antibacterial effect.

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Production of Bacteriorhodopsin by Halobacterium halobium in the Internal Membrane Bioreactor (내부 필터 발효기에서 Halobacterium halobium의 배양에 의한 박테리오로돕신의 생산)

  • 엄영순;박준택;홍순호;이상엽;장호남
    • KSBB Journal
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    • v.13 no.3
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    • pp.268-271
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    • 1998
  • Bacteriorhodopsin in the purple membrane (PM) of halobacteria has recently been attracting much attention to be used as a component of molecular electron device and optical computers. In order to increase the productivity of bacteriorhodopsin in high cell density cultures of Halobacterium halobium R1, an internal membrane cell-retention bioreactor system was employed. As a result, the production of cell mass at OD660 of 12 and of bacteriorhodopsin at 125-130 mg/L were obtained using the internal membrane bioreactor system at a dilution rate of 0.066 hr-1. The productivity achieved by the internal membrane system (0.7 mg/L$.$hr) was 3.5-fold higher than that obtained by the corresponding batch cultivations (0.2 mg/L$.$hr).

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Characteristics of Phenol Degradation in Wastewater Treatment using Packed bed reactor (충진층 반응기를 이용한 폐수처리에서 페놀의 분해 특성)

  • 염승호;최석순
    • Journal of environmental and Sanitary engineering
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    • v.11 no.3
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    • pp.13-19
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    • 1996
  • Packed bed reactor containing immobilized microorganisms which degraded phenol without growth was used to remove phenol from the synthetic wastewater. The effects of temperature, retention time(reactor volume/flow rate) and phenol concentration on the removal efficiency of phenol were investigated. The effect of temperature in the range of 20-30$\circ $C was negligible while retention time and phenol concentration influenced the removal of phenol significantly. When retention time was in the range of 1-1.5 hour, the removal efficiency of phenol was affected not by phenol concentration but by retention time itself while it was influenced by phenol concentration above 1.5 hour of retention time. The beads after 720 hours operation were swelled by 40 % in diameter which could be prevented by crosslinking with glutaraldehyde at the expense of cell activity.

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Enhancement of Data Retention Time in DRAM through Optimization of Sidewall Oxidation Precleaning (측면산화 프리크리닝의 최소화를 통한 DRAM의 데이터 유지시간 개선)

  • Chai, Yong-Yoong;Yoon, Kwang-Yeol
    • The Journal of the Korea institute of electronic communication sciences
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    • v.7 no.4
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    • pp.833-837
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    • 2012
  • This paper proposes a DRAM data retention time enhancement method that minimizes silicon loss and undercut at STI sidewall by reducing the SC1 (Standard Cleaning) time. SC1 time optimization debilitates the parasitic electric field in STI's top corner, which reduces an inverse narrow width effect to result in reduction of channel doping density without increasing the subthreshold leakage of cell Tr. Moreover, it minimizes the electric field in depletion area from cell junction to P-well, increasing yield or data retention time.

Optimal Volume Estimation for Non-point Source Control Retention Considering Spatio-Temporal Variation of Land Surface (지표면의 시공간적 변화를 고려한 비점오염원 저감 저류지 최적용량산정)

  • Choi, Daegyu;Kim, Jin Kwan;Lee, Jae Kwan;Kim, Sangdan
    • Journal of Korean Society on Water Environment
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    • v.27 no.1
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    • pp.9-18
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    • 2011
  • In this study the optimal volume for non-point source control retention is estimated considering spatio-temporal variation of land surface. The 3-parameter mixed exponential probability density function is used to represent the statistical properties of rainfall events, and NRCS-CN method is applied as rainfall-runoff transformation. The catchment drainage area is divided into individual $30m{\times}30m$ cells, and runoff curve number is estimated at each cell. Using the derived probability density function theory, the stormwater probability density function at each cell is derived from the rainfall probability density function and NRCS-CN rainfall-runoff transformation. Considering the antecedent soil moisture condition at each cell and the spatial variation of CN value at the whole catchment drainage area, the ensemble stormwater capture curve is established to estimate the optimal volume for an non-point source control retention. The comparison between spatio-temporally varied land surface and constant land surface is presented as a case study for a urban drainage area.

Effect of Charge Carrier Lipid on Skin Penetration, Retention, and Hair Growth of Topically Applied Finasteride-Containing Liposomes

  • Lee, Sang-Im;Nagayya-Sriraman, Santhosh-Kumar;Shanmugam, Srinivasan;Baskaran, Rengarajan;Yong, Chul-Soon;Yoon, Sang-Kwon;Choi, Han-Gon;Yoo, Bong-Kyu
    • Biomolecules & Therapeutics
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    • v.19 no.2
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    • pp.231-236
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    • 2011
  • The aim of this study was to investigate the effect of charge carrier lipid on the skin penetration, retention, and hair growth of topically applied finasteride-containing liposomes. Finasteride-containing liposomes were prepared by traditional thin film hydration method using Phospholipon$^{(R)}$ 85 G and cholesterol with or without charge carrier lipid (1,2 dimyristoyl-sn-glycero-3-phosphate or 1,2-dioleoyl-trimethylammonium-propane for anionic and cationic charge, respectively). Freshly prepared finasteride-containing liposome suspension was applied on the hairless mouse skin, and skin penetration and retention were measured using Keshary-Chien diffusion cell. Non-liposomal formulation (ethanol 10% solution containing 0.5 mg/ml of FNS) was also used as a control. The amount of finasteride in the diffusion cell and mouse skin was measured by HPLC. The hair growth was evaluated using depilated male C57BL/6N mice. Mean particle size of all finasteride-containing liposomes was less than a micron, and polydispersity index revealed size homogeneity. Skin penetration and retention studies showed that significantly less amount of finasteride was penetrated when applied as anionic liposome while more amount of the drug was retained. Specifically, in liposome prepared with 10% anionic charge carrier lipid, penetration was 12.99 ${\mu}g/cm^2$ while retention was 79.23 ${\mu}g/cm^2$ after 24 h of application. In hair growth study, finasteride-containing anionic liposomes showed moderate efficacy, but the efficacy was not found when applied as cationic liposomes. In conclusion, topical application of finasteride using anionic liposome formulation appears to be useful option for the treatment of androgenetic alopecia to avoid systemic side effects of the drug.

A HISTOLOGIC STUDY OF PULPAL REACTION AFTER EXPERIMENTAL TOOTH MOVEMENT IN RATS (백서구치의 실험적 치아이동후 치수조직 변화에 관한 조직학적 연구)

  • Jin, Keun Ho;Hong, Sung Joon
    • The korean journal of orthodontics
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    • v.21 no.3
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    • pp.635-656
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    • 1991
  • It was the aim of this investigation to evaluate some histologic aspect of rat pulp tissue after it had been compromised by an experimental orthodontic force. Experimental animals of thirty five Spraque-Dawley rats were employed. The first upper molars had been successively mesial moved (initial load 100 gr.) with a closed coil spring during 21 days. The experimental periods were set on immediate, 1 day, 1 week, 2 weeks, 3 weeks, 4 weeks following retention time. On each experimental period, the rats were killed and prepared for the light microscopy. After prepared with H/E stain and Gomori's one-step trichrome stain, the specimens were analyzed with evaluation criteria which were adopted in this study. The result may be summarized as follows; 1. The main pulp changes due to experimental orthodontic force included vacuolization of odontoblastic layer, circulation disturbance, root resorption, reduced pulp collagenous fiber density and mean cell count of pulp fibroblast in the immediate group. 2. The pulp tissue changes were revealed reversible because the relieved pulp tissues from experimental orthodontic force were recovered rapidly in each evaluation criteria during retention periods. 3. Compared with normal control group, pulp collagenous fiber density were decreased in immediated group (p < 0.01), but increased in each retention groups. These seem to suggest that the pulp tissues were aged after experimental orthodontic force conditions. 4. Compared with normal control group, mean cell counts of pulp fibroblasts were decreased in immediate group (p < 0.05), but increased continuous in each retention groups. These seem to indicate that the pulp tissues were highly regenerative after experimental orthodontic force conditions. 5. Compared with normal control group, root resorptions occurred in all immediate specimens (p < 0.01) and they were healed in each retention periods, but often observed in 4 weeks retention group. These seem to indicate that root resorptions were recovered slowly after experimental orthodontic force conditions.

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Effect of Temperature on the Deterioration of Graphite-Based Negative Electrodes during the Prolonged Cycling of Li-ion Batteries

  • Yang, Jin Hyeok;Hwang, Seong Ju;Chun, Seung Kyu;Kim, Ki Jae
    • Journal of Electrochemical Science and Technology
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    • v.13 no.2
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    • pp.208-212
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    • 2022
  • In this paper, we report the effects of temperature on the deterioration of graphite-based negative electrodes during the longterm cycling of lithium-ion batteries (LIBs). After cycling 75 Ah pouch-type LIB full cells at temperatures of 45℃ (45-Cell) and 25℃ (25-Cell) until their end of life, we expected to observe changes in the negative electrode according to the temperature. The thickness of the negative electrode of the cell was greater after cycling; that of the electrode of 45-Cell (144 ㎛) was greater than that of the electrode of 25-Cell (109 ㎛). Cross-sectional scanning electron microscopy analysis confirmed that by-products caused this increase in the thickness of the negative electrode. The by-products that formed on the surface of the negative electrode during cycling increased the surface resistance and decreased the electrical conductivity. Voltage profiles showed that the negative electrode of 25-Cell exhibited an 84.7% retention of the initial capacity, whereas that of 45-Cell showed only a 70.3% retention. The results of this study are expected to be relevant to future analyses of the deterioration characteristics of the negative electrode and battery deterioration mechanisms, and are also expected to provide basic data for advanced battery design.

Performance of Microbial Fuel Cell Integrated with Anaerobic Membrane Filter for Continuous Sewage Treatment with Stable Effluent Quality (안정적 유출수질의 연속 하수처리를 위한 혐기성 멤브레인 필터와 통합된 미생물연료전지의 성능 평가)

  • Lee, Yunhee;Oa, Seong-Wook
    • Journal of Korean Society on Water Environment
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    • v.29 no.6
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    • pp.808-812
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    • 2013
  • A new type of microbial fuel cell (MFC) with anaerobic membrane filter was designed to produce bioelectricity and to treat domestic sewage at relatively high organic loading rate (OLR) of $6.25kgCOD/m^3/day$ and short hydraulic retention time (HRT) of 1.9 h. A following aeration system was applied to ensure effluent water quality in continuous operation. Glucose was supplemented to increase the influent concentration of domestic sewage. Influent substrate of 95% was removed via the MFC and following aeration system and the corresponding maximum power density was $25.6mW/m^3$. External resistor of $200{\Omega}$ and air-cathode system contributed better MFC performance comparing to $2000{\Omega}$ and dissolved oxygen as a catholyte.

The Effect of high Carbohydrate and Cellulose Diets on the Growth of Albino Rate (High Carbohydrate 와 Cellulose Diet가 흰쥐의 체내대사에 미치는 영향 ( I ))

  • Yu, Choon-Hie;Kim, Sook-He
    • Journal of Nutrition and Health
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    • v.9 no.2
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    • pp.24-39
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    • 1976
  • The present study was undertaken to investigate the metabolic problems of high carbohydrate and cellulose diets of Korean. Forty males and same number of females of Albino rats, aged $45{\pm}5$days were divided into 95% high carbohydrate (H. CHO)group, 83.8% medium carbohydrate (M. CHO)group, 50% low carbohydrate (L. CHO) group and standard (Stand). group containing 72.2% sugar. Each group was divided into two again-1.55% cellulose group and non-cellulose group, 10 rats each of eight groups in both sexes. Cellulose was added to each of non-cellulose diets in the forms of spinach powder and rice bran. After 14 weeks the rats were sacrificed for chemical analysis and the results were elucidated as follows. (1) H. CHO+Cell. group showed the lowest value in body weight gained and shrinkage of almost all organs, in contrast with this group the L.CHO group showed higher body weight gained than Stand. group. M. CHO+Cell, group showed much the same body weight gained curve as Stand. group. (2) It was observed that cellulose group showed lower F.E.R and P.E.R value than non-cellulose group comparatively. (3) Total nitrogen retention and retention rate were decreaced in H. CHP groups compared with M. CHO or L. CHO groups. (4) The amount of feces was increased due to addition of cellulose to experimental diets and in accordance with the increasing total fecal excretion of nitrogen and glucose was also increased, especially noticeable in fecal glucose excretion. (5) It was noteworthy that serum cholesterol level was decreased due to addition of cellulose in H. CHO group and L. CHO group. (6) M. CHO+Cell. group was designed to reflect the average survey data of Korean diets and there was no significant differences on body weight gained, F.E.R, P.E.R, total nitrogen retention and hematology between M. CHO+Cell. and Stand. group. Total glucose excretion was increased due to dietary cellulose in M. CHO+Cell. group, but it seemed to be no metabolic problems in this group.

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