• Journal of Applied Biological Chemistry
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• 제66권
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• pp.424-435
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• 2023

본 연구 결과 DPPH 라디칼 소거 활성은 갈랑가 뿌리 70% 에탄올 추출물(AG.E) 100 ㎍/mL 농도에서 81.8%이었으며 ABTS⁺ 라디칼 소거 활성은 AG.E 50 ㎍/mL의 낮은 농도에서 L-Ascorbic acid (AA)와 유사한 99.8%로 확인되었다. 항노화 활성을 측정하기 위하여 collagenase와 elastase 저해 활성을 측정한 결과 둘 모두에서 AG.E는 50 ㎍/mL의 낮은 농도부터 epigallocatechin (EGCG)보다 더 높은 저해 효과를 나타내었다. 특히 500 ㎍/mL 농도에서 EGCG 대비 3배 이상의 저해 효과를 보였다. CCD-986sk 세포내에서 AG.E의 항노화 효과를 검증하기 위해 UVB로 자극한 다양한 실험에서도 우수한 항노화 효과가 얻어졌다. RT-PCR을 통한 유전자 발현 분석 실험에서 COL1A mRNA 발현량은 AG.E 20 ㎍/mL 저농도에서 무첨가 대비 2.90배 증가시키는 결과가 얻어져 항노화관련 우수한 기능성 소재로 개발 가능성이 확인되었다. 제형에 소재의 적용 시 생리활성의 경시적 보전성에 대한 기초 연구로서 AG.E 및 AA등을 안정한 W/O type emulsion에 첨가하여 25 ℃ 항온조에 보관하면서 1일차, 30일차, 60일차에 DPPH와 ABTS+ 라디칼소거 활성을 측정한 결과 모두 경시적으로 항산화 효과가 높은수준 유지됨을 확인하였다.

• 한국조리학회지
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• 제18권2호
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• pp.162-171
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• 2012

가공식품의 개발에 있어서 식품의 맛과 더불어 저장성, 열안정성 및 색도유지는 소비자의 기호도에 중요한 영향을 미친다. Protopectinase는 식물조직 중엽부의 성분인 불용성 proto-pectin을 분해하여 단세포화하는 효소이다. Protopectinase를 포도에 적용시켜 포도 고유의 세포속에 함유되어 있는 세포내 성분들을 파손없이 단세포로 유리하였다. pH, 총산 함량, 총 폴리페놀함량 및 항산화력은 기계 처리구보다 효소 처리구에서 보다 안정성을 나타내었으며 이는 단세포 처리에 의하여 이들 성분이 안정하게 유지됨을 알 수 있었다. Protopectinase로 처리된 포도 단세포화물을 $4^{\circ}C$에서 28일간 저장하며 색도를 관찰한 결과, 단세포 처리구에서는 기계 처리구보다 색도의 차이가 미비하였으며 기계 처리구에서는 단세포 처리구보다 색의 변화가 크게 나타났다. 또한 포도 단세포화물을 $80^{\circ}C$에서 30분간 열처리한 후 관찰한 결과, 기계 처리구의 경우가 단세포 처리구의 경우보다 품질의 변화를 크게 나타내어 이러한 결과는 효소적 단세포화물의 열 안정성을 의미한다. 따라서 Protopectinase를 이용한 포도의 단세포화는 과실음료제조 및 원료보존에 유용하게 응용가능하며, 나아가 포도 단세포화물의 폭넓은 식품 소재화 가능성과 고부가가치 기능성 식품제조에 이용 될 수 있음을 의미한다.

• 한국발생생물학회지:발생과생식
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• 제11권2호
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• pp.79-84
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• 2007

냉동보존이 진주조개(Pincata fucata martensii) 유생의 형태 및 구조에 미치는 영향을 알아보기 위하여 냉동전후 D형 및 각정기 유생을 광학 및 전자현미경으로 조사하였다. 동해방지제는 0.2 M sucrose를 첨가한 2.0 M $Me_2SO$를 사용하였다. 냉동후 유생은 일부 패각이 손상되긴 했지만 hinge와 prodissoconch가 뚜렷하게 나타났으며, 소포체, 지질 과립, 미토콘드리아, 핵 등을 포함한 세포내 소기관들이 고르게 분포되어 있었다. 또한, 섬모가 규칙적으로 배열되어 있었고, 섬모 아래 미토콘드리아와 지질과립이 위치해 있는 것이 관찰되었으나, 일부 해동된 유생에서 섬모의 불규칙적인 배열과 섬모환이 둥글게 뭉쳐져 있는 모습이 관찰되었다. 이러한 결과는 진주조개의 D형 유생과 각정기 유생이 냉동에 쉽게 영향을 받을 수 있다는 것을 보여준다. 따라서 냉동보존 시 세포의 손상을 감소시킬 수 있는 연구가 이루어져야 할 것으로 사료된다.

• 대한두경부종양학회지
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• 제12권1호
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• pp.26-31
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• 1996

Radiation therapy in T1 glottic cancer offers an excellent cure rate with preservation of voice. From 1983 to 1992 eighty nine patients with TNM staged T1N0M0 invasive squamous cell carcinoma of the glottis were treated at the Dept. of Radiation Oncology, Yonsei Cancer Center, Yonsei University. There were 84 men and 5 women with median age of 59 years. All patients were treated either with Co-60 teletherapy unit or 4MV linear accelerator with an median dose of 6400 cGy(6000-7000 cGy), 200 cGy per day, 5 days in a week. Fourteen local failures have been observed and the median time to local recurrence was 17 months. There were no nodal failure without local recurrence or distant metastases. The 5 year local control rate was 84.3%. The 5 year actuarial surivival rate and the 5 year disease free survival rate were 89.2%, 87.5%, respectively. The 5 year actuarial survival rate and the 5 year disease free survival rate of the nineteen patients with anterior commissure involvement were 77.8% and 74.5% which were lower than those of seventy patients without anterior commissure involvement(91.6%, 90.6%)(p < 0.05). Among the several influencing factors, anterior commissure involvement was the significant prognostic foctor. Final local control rate, taking into account the salvage surgery, was 89.9% at 5 years.

• Journal of Chest Surgery
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• 제32권11호
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• pp.978-983
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• 1999

Background: Complement activation with transpulmonary leukocyte sequestration is considered a main mediator leading to ischemia-reperfusion lung(I-R) injury. We studied the role of leukocytes in the formation of I-R injury in ovine cardiopulmonary bypass(CPB) model with a membrane oxygenator. Material and Method: Five sheep were used. CPB circuitry consisted of a roller pump(American Optical Corp., Greenwich, CT, USA) and a membrane oxygenator(UNIVOX-IC, Bentley, Baxter Health Corp, Irvine, CA, USA). The CPB time was fixed at 120 min. Ten minutes after the start of CPB, total CPB was established. Thereafter a total CPB of 100 min was performed, followed by another 10 min of partial CPB. The CPB was discontinued and the animals were fully recovered. For measuring left and right atrial leukocyte counts, blood samples were taken before thoracotomy, 5 min and 109 in after the start of CPB, and 30 min and 120 min after weaning. C3a was measured before thoracotomy, 109 min after the start of CPB, and 30 min and 120 min after weaning. Plasma malondialdehyde(MDA) was checked before thoracotomy, 109 min after the start of CPB, and 30 min after weaning. One to two grams of lung tissue were taken for water content measurement before thoracotomy, 109 min after the start of CPB, and 30 min after weaning. Lung biopsy specimens were examined by light and electron microscopy. Result: Of 5 animals, 4 survived the experimental procedures. Of these, 3 animals survived on a long-term basis. No significant differences in transpulmonary gradients of leukocyte were found and no significant complement activation was expressed by C3a levels. MDA level did not show significant changes related to lung reperfusion despite an increase after the start of CPB. On both light and electron microscopic examinations, mild to moderate acute lung change was observed. Interstitial edema, leakage of erythrocytes into the alveolar space and endothelial cell swelling were the main findings. Water content of the lung showed a slight increase after the start of CPB, but there was no statistical significance. Conclusion: These findings indicate that ischemia-repersusion lung injury may not be from complement activation-leukocyte sequestration but from another source of oxygen free radicals related to CPB.

• 유기물자원화
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• 제11권4호
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• pp.114-119
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• 2003

본 연구는 남은 음식물의 사료화를 목적으로 Lactobacillus acidophilus와 Saccharomyces cerevisiae를 이용하여 남은 음식물을 발효시켜 고품질의 생균사료를 생산하기 위하여 실시하였다. 액상으로 충분히 마쇄시킨 남은 음식물 시료에 공기주입량을 0v.v.m 0rpm, 0.25v.v.m 100rpm, 0.5v.v.m 200rpm과 1v.v.m 500rpm으로 다르게 조절하여 $30^{\circ}C$에서 발효시킨 결과 0.5v.v.m 200rpm의 조건에서 생균수가 $4.5{\times}10^9CFU/m{\ell}$로 가장 높은 것으로 나타났다. 또한 공기주입량을 조절함으로써 최종 발효물의 pH를 조절할 수 있었고 사료로서의 기호성에 알맞고 보존성도 좋은 4-5의 pH범위를 얻을 수 있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제8권4호
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• pp.173-179
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• 2004

Amyloid ${\beta}-peptide\;(A{\beta})$ contributes to the pathogenesis of Alzheimer's disease (AD), causing neuronal death through apoptosis. In this study, the neuroprotective role of BF-7, extracted form sericultural product, was examined against $A{\beta}-induced$ toxicity in cultured human neuronal cell SKN-SH. In order to know if the BF-7 has positive role on the cognition and memory in human, the mixture of BF-7, DHA and EPA (BDE) was examined using Rey Kim and K-WAIS test with 50 healthy high school student. We report here that BDE significantly attenuated $A{\beta}-induced$ apoptosis through the reduction of ROS accumulation, and diminished caspase-like protease activity. Moreover, the memory index and memory preservation, and attentative concentration of BDE treated group for 1 month were significantly improved, in contrast to the case of placebo control treated with DHA and EPA. This result represent that the BF-7 play significant positive role on learning memory. Taken together, our result suggested the natural product BF-7 is a good substance for the brain functionally and physiologically.

• 대한두경부종양학회지
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• 제18권1호
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• pp.46-49
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• 2002

Background: Free flaps have become a very important role in the ablative head and neck surgery with functional preservation of defect sites. The forearm free flap has many advantage of lack of bulk, ease of dissection, vascularity, and malleability. Patients and Methods: A review of 51 radial forearm free flaps performed between may 1990 and feburary 2001 at the Korea University was undertaken to determine outcome. Patient age ranged 27 to 72 years (mean 56). There were 44 men and 7 women. The most prevalent neoplasm was squamous cell carcinoma. Results: The tans verse cervical artery and the external jugular vein were the most frequent receipient sites for microvascular anastomosis. Total flap loss occurred in 2 cases (4%) and other complications were seen in 42%. Fifteen patients received preoperative irradiation and the complication was higher than non-irradiation patients, but statistically not significant. Conclusion: The radial forearm free flap offers a variety of reconstructive options for head and neck. Its low flap loss and complication rates offer the best choice for reconstruction of defect of head and neck malignancy.

• 한국임상수의학회지
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• 제14권1호
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• pp.56-64
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• 1997

These studies were performed to investigate the complications affecting the vision after extracapsular lens extraction(ECE), the effects of an I/A (irrigation & aspiration)device and a viscoelastic material used on the vision, the occurrence of complications and the effective corneal incision method to reduce the corneal opacity in dogs. ECE was performed bilaterally with 3 different methods using clinically normal twele mixed dogs; the method in which I/A device and viscoelastic material were not used, the method in which I/A device was used but viscoelastic material not, and the method in which I/A device and viscoelastic material were used. Postoperative complications were observed as followed; conjunctival injection, uveitis, corneal opacity due to endothelial cell loss, hyphemia, remnants of lens cortex, vitreous loss, synechia and capsular opacity. Preservation rate of vision was lower significantly in the cases showing signs of synechia, capsular opacity, or remnants of lens cortex than the cases not showing the above signs(p<0.01). There were significant reduction of the complications such as corneal opacity, clot in anterior chamber in the group using I/A device compared to the group in which I/A device was not used(p<0.01). Groups using I/A device showed slightly higher vision than the group not using I/A device (75%; 42%). There were no significant differences in the occurrence rate of complications and the preservative rate of vision between the groups with and without viscoelastic material. The present study indicated that the postoperative complications of posterior synechia, capsular opacity, uveitis and vistreous loss were important factors affecting the vision and that I/A device was applicable to extract the lens cortex and effective to elevate the success rate after ECE in dogs.

• The Korean Journal of Physiology and Pharmacology
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• 제1권4호
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• pp.345-353
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• 1997

The effects of crude saponin (SAP) and alkaloid (ALK) fractions of Panax ginseng C.A. Meyer on the detrimental effects of electroconvulsive shock (ECS) and scopolamine on passive avoidance response (PAR) were studied in male Sprague-Dawley rats, referring their effects on the neuronal injury and plasticity of hippocampus in response to electrolytic lesion of left entorhinal cortex (ECL). The detrimental ECS effect on PAR was attenuated by pre- and post-treatments with SAP and ALK, respectively, or by pretreatment with aminoguanidine (AG), an inhibitor of diamine oxidase and NO synthase. And the detrimental scopolamine effect on PAR was also inhibited by pre-treatment with ALK or AG, and by post- treatment with SAP or ALK, respectively. On the 7th day after ECL, the brain sections stained by cresyl violet and by acetylcholinesterase (AChE) histochemistry, respectively, showed the chromatolysis and numeral decrease of neurons and the reduction of AChE reactivity in the hippocampus CA1 area and to a lesser extent, in the dentate gyrus. The neuronal cell death of the CA1 area was significantly reduced by SAP, ALK, or AG, and the reduction of AChE reactivity was significantly attenuated by SAP or ALK and to a lesser extent by AG. These results suggests that the protective effect of ginseng SAP and ALK fractions on ECS- or scopolamine-induced impairment of PAR may be ascribed in part to preservation of hippocampal neurons, particularly cholinergic neurons.