This study is conducted to analyze the difference among college students' department satisfaction, career maturity, and career stress, and to examine the influence of department satisfaction and career maturity on career stress. A survey was done among 824 college students using the scales on department satisfaction, career maturity, and career stress and the following results were found. First of all, department satisfaction, career maturity, and career stress had statistically significant differences based on students year, age, academic records, entrance motive, peer relation, and health condition. Secondly, the subfactors of department satisfaction, namely, course satisfaction, relationship satisfaction, general satisfaction, and recognition satisfaction, showed statistically significant differences according to age, gender, academic records, entrance motive, peer relation, and health condition. Thirdly, the subfactors of career maturity, namely, determinacy, participation, independence, tendency, and compromise, had statistically significant differences according to year and age. Lastly, career maturity and department satisfaction considerably influenced career stress: when department satisfaction and career maturity were lower, career stress was higher. These results indicate that department satisfaction and career maturity correlate with career stress. Therefore, in order to decrease career stress, college students who maintain a low level of department satisfaction and career maturity should receive active guidance and education.