• Title/Summary/Keyword: Capillary method

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Analysis of Branched PEG-Conjugated Interferon Alpha by Capillary Electrophoresis and MALDI- TOF Mass Spectrometry

  • Na, Dong-Hee;Park, Eun-Ji;Lee, Snag-Deuk;Jo, Young-Woo;Lee, Sung-Hee;Kim, Won-Bae;Lee, Kang-Choon
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.406.3-407
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    • 2002
  • Analysis of interferon alpha (IFN) modified with high molecular weight branched PEG was performed by capillary electrophoresis (CE) and MALDI-TOF mass spectrometry (MALDI-TOF MS). IFN was modified by the reaction of amine residues with an active ester of monomethoxy polyethylene glycol at various molar ratios. As a CE method. capilary electrophoresis sodium dodecyl sulfate nongel sieving (CE-SDS NGS) was performed using an uncoated capilary filled with a hydrophilic replaceable polymer network matrix. (omitted)

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Recent Advances in DNA Sequencing by End-labeled Free-Solution Electrophoresis (ELFSE)

  • Won, Jong-In
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.3
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    • pp.179-186
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    • 2006
  • End-Labeled Free-Solution Electrophoresis (ELFSE) is a new technique that is a promising bioconjugate method for DNA sequencing (or separation) and genotyping by both capillary and microfluidic device electrophoresis. Because ELFSE enables high-resolution electrophoretic separation in aqueous buffer alone (i.e., without a polymer matrix), it eliminates the need to load viscous polymer networks into electrophoresis microchannels. To achieve microchannel DNA separations with high performance, ELFSE requires monodisperse perturbing entities (i.e., drag-tags), which create a large amount of frictional drag when pulled behind DNA during free-solution electrophoresis, and which have other properties suitable for microchannel electrophoresis. In this article, the theoretical concepts of ELFSE and the required characteristics of the drag-tag molecules for the ultimate performance of ELFSE are reviewed. Additionally, the merits and limitations of current drag-tags are also discussed in the context of recent experimental data of ELFSE separation (or sequencing).

manufacturing micro CPL (Capillary Pumped Loop)by using LIGA process (LIGA process를 이용한 micro CPL(Capillary Pumped Loop)제작)

  • Cho, Jin-Woo;Jung, Suk-Won;Park, Joon-Shik;Park, Sun-Seob
    • Proceedings of the KIEE Conference
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    • 2001.07c
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    • pp.1881-1883
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    • 2001
  • We manufactured a micro CPL by LlGA process, a new conceptual ultra-fine and precise forming method, using X-ray lithography process. We fabricated a BN X-ray mask having properties of good X-ray transmittance and large mechanical strength. Micro CPL was manufactured by dividing into an upper plate and a low plate. Each of plates was bonded by Ag paste screen printing. The upper plate was fabricated on glass wafer to observe flow and phase transformation of cooling solution. The lower plate was manufactured by Cu electroplating for good heat transmission. Precision of inner Parts, micro pin and micro channel, of manufactured micro CPL is under ${\pm}2{\mu}m$.

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Sound Propagation through the Diesel Particulate Filter (DPF) (디젤 매연여과기 (DPF) 내에서의 음향전파)

  • Choi, Won-Yong;Ih, Jeong-Guan
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2005.11b
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    • pp.152-155
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    • 2005
  • Diesel particulate filter (DPF) is comprised of a number of capillary tubes enclosed by porous ceramic wails, shaped like a plugged duct. Hot gas flows through the DPF along with the exhaust noise from Diesel engine. Based on previous works on the sound propagation through DPF, in this study, losses at entrance, exit, and ceramic walls are considered and the gradients in temperature and flow velocity are considered. Transfer matrix at entrance, monolith, and exit parts are obtained by employing the segmental approach in analyzing the sound propagation through DPF. The predicted transmission loss agrees very well with the empirical one, which is measured by the improved method with correction terms.

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Determination of Arylmethyl Halides by Gas Chromatograph (Gas Chromatograph에 의한 Arylmethy Halides의 정량)

  • Oh, Dos Suk;Choi, Ho Chun
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.4 no.1
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    • pp.25-32
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    • 1994
  • Arylmethyl halides(benzyl chloride, BZYC; benzal chloride, BZYA; benzotrichloride, BZTC) and related compounds(benzyl alcohol, BZYA; benzoyl chloride, BZOC) were determined by GC using capillary column. Detailed results are as follows. 1. Stability In methanol benzotrichloride slowly transferred to benzoyl chloride by hydrolysis(ca. 7.5% for 5 days), but the others were stable. Therefore, benzotrichloride solution should be prepared just before analysis. 2. GC analysis Tenax-GC was used to absorbent and desorption solvents were $CCl_4$ and MeOH. Arylmethyl halides were analyzed within 7.5min without interference with related compounds. The calibration curve(ca. 15-80 ppm in soln), repeatability(n=10) and the desorption efficiency were good. Limit of detection by NIOSH method was about 0.003 ppm for arylmethyl halide, respectively. To analyze arylmethyl halides and related compounds in working places GC using capillary column is anticipated to be used effectively.

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A Study on the Characteristics of Stiction and Friction of Texture Surface (표면 구조 변화에 따른 응착과 마찰 특성에 관한 연구)

  • Yang, Ji-Cheol;Kim, Dae-Eun
    • Journal of the Korean Society for Precision Engineering
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    • v.19 no.7
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    • pp.51-58
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    • 2002
  • In this paper, the characteristics of silicon wafer surface which is textured by KOH anisotropic etching method and mechanical polishing are investigated from the viewpoint of stiction and friction. It was found that the characteristics of stiction and friction of each textured surface are dependent on the contact area characterized by surface parameters such as bearing length ratio and peak count. To find the mechanism of the variation of stiction and friction in textured surface, OTS SAM coated wafer was used. In this case, the variation of stiction and friction was diminished, Therefore, it is concluded that the reason of variation of stiction and friction on textured surface is mainly caused by capillary which in turn is affected by the surface topography

?Experimental Study on the Effects of Zanthoxyli Fructus and Fagarae mandshuricae Fructus (천초(川椒) 및 산초(山椒)의 효능(?能)에 관(關)한 실험적(實驗的) 연구(硏究))

  • O, Han-Gyun;Won, Jong-Hun;Jeong, Gyu-Man
    • The Journal of Pediatrics of Korean Medicine
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    • v.4 no.1
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    • pp.1-17
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    • 1990
  • ?In order to investigate effects of Zanthoxyli Fructus (Sample-A) and Fagarae mandshuricae Fructus (Sample-B), experimental study was performed through observation on isolated - ileum analgesic, hypothermal, anti-pyretic, anti-inflammatory, capillary permeability, blood flow rate, blood pressure and respiratory works. The results were as follows; 1. In spontaneous movement of mice isolated-ileum, both samples were noted to have strong inhibitory action after temporary contraction, and to show antagonism against acetylcholine chloride and barium chloride. 2. Both samples were showed to have antihistamin work on guinea pig isolated-ileum 3. In analgesic work by the acetic acid method, only Fagarae mandshuricae Fructus was proved to have significant analgesic effect in mice. 4. Both sample groups indicated anti-pyretic and anti-inflammatory effect. 5. Both samples were noted to have inhibitory effect against capillary permeability ?rise in mice. ?6. Both samples were noted to have dose-dependent falling effect on blood pressure in rabbits.

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The Analysis of VOCs by GC/MS with Whole Column Coldtrapping on a Fused Silica Capillary Column in Indoor Environment

  • Dai, Shugui;Zhang, Lin;Bai, Zhipeng
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.829-834
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    • 1995
  • Whole column coldtrapping(WCC) on a fused silica capillary (FSCC)combined with GC/MS analysis was evaluated for use in the investigation of volatile organic compounds(VOCs) in indoor air. Research had indicated that a temperature of $-80^{\circ}C$ is optimal for WCC. Samples were analyzed on a $50m{\times}0.2mm$ cross-linked 5% phenylmethylsilicone fused silica column. Liquid nitrogen was used as the coolant for the peak resolution significantly. The analysis can be performed quickly and conveniently. More than 112 of VOCs were determined in the samples from three typical indoor environment including: (1) a room which had just been decorated involving building materials and paints; (2)a kitchen used for Chinese cooking, and (3) a room had tobacco smoke. The method is could be readily applied to rapid sample screening for VOCs contamination surveys or initial investigations with its valid and simple sampling and analytical technique.

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Capillary Electrophoresis Detection of Hydrogen Peroxide by Using Titanium Ion and 4-(2-thiazolylazo)resorcinol

  • Vu Phuong, Dong;Yoo, Hoon
    • International Journal of Oral Biology
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    • v.42 no.4
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    • pp.197-201
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    • 2017
  • A novel method for the detection of hydrogen peroxide in aqueous solution was developed via reaction between $H_2O_2$, trivalent titanium ion ($Ti^{3+}$) and 4-(2-thiazolylazo) resorcinol (TAR), resulting in a ternary complex with a maximum UV absorbance at 530 nm. The CE detection of $H_2O_2$ was fast, sensitive and cost-effective without pretreatment procedures. $H_2O_2$ was detected within 15 min at 1 to $100{\mu}M$ range with the lowest detection limit at $1.0{\mu}M$. Under the optimized CE conditions, the concentration of $H_2O_2$ in coffee or tea extract was quantitatively determined. Our results show that CE detection of the ternary complex of $H_2O_2-Ti^{3+}$-TAR has potential applications for the detection of $H_2O_2$ in aqueous sources.

Theoretical Analysis of the Pressure Drop in Loop Heat Pipe by Sintered Porous Wick Structure (다공성소결윅구조에 따른 루프 히트파이프에서 압력손실의 이론적 분석)

  • Lee, K.W.;Lee, W.H.;Park, K.H.;Lee, K.J.;Chun, W.P.;Ihn, H.M.
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.1225-1230
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    • 2004
  • In this paper, the pressure drops were investigated according to the sintered porous wick structure in loop heat pipe(LHP) by theoretical analysis. LHP has the wick only in evaporator for the circulation of working fluid, so utilizes porous wick structure which pore diameter is very small for large capillary force. This paper investigates the effects of different parameters on the pressure drops of the LHP such as particle diameter of sintered porous wick, wick porosity, vapor line diameter, thickness of wick and heating capacity. Working fluid is water and the material of sintered porous wick is copper. According to the these different parameters, capillary pressure, pressure drop in wick were analized by theoretical design method of LHP.

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