• Title/Summary/Keyword: Cadmium resistance

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Characteristics of Cadmium-Resistant Bacillus subtilis DT134 (Bacillus subtilis DT134의 카드뮴 저항성)

  • 윤경표
    • KSBB Journal
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    • v.13 no.4
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    • pp.383-390
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    • 1998
  • Bacillus subtilis DT134 was resistant to 50-fold higher concentration of cadmium ions (Cd2+) than cadmium-sensitive B. subtilis BD224 in Luria Broth (LB) medium. Minimal inhibition concentration test in LB agar plates also showed similar results. The elevated cadmium resistance of B. subtilis DT134 strongly suggested a possible existence of cadmium resistance gene in it. Southern blot with Staphylococcus aureus cadA gene fragment (757 bp NlaIV-XmnI cadA DNA fragment) as probe was carried out to test the existence and similarity of the gene. In high stringency condition, there was no detectable signal, but in low stringency, a strong signal specific to the cadA probe could be detected. These results strongly suggested that there was some similarity between total DNA of B. subtilis DT134 and S. aureus pl258 in terms of cadmium resistance gene and the resistance mechanism might be an efflux mechanism. The subsequent efflux experiment showed that the cadmium resistance mechanism of B. subtilis DT134 was also due to the efflux of cadmium.

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Plasmid-Determined Cadmium Resistance in Cocobacilli Strain B-17 Isolated from Soil. (토양에서 분리된 Cocobacilli B-17균의 Plasmid가 결정하는 Cadmium내성)

  • 방병호
    • The Korean Journal of Food And Nutrition
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    • v.1 no.2
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    • pp.64-67
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    • 1988
  • Cadmium resistant cocobacillus B-17 from soil was tolerated up to 1600ug/ml of cadmium at agar plate and the strain B-17 was able to grow at 600ug/ml of cadmium at liquid medium after the lag phase being prolonged with lengthening culture time. Optimal pH of the strain was shown at pH7.0. The elimination frequency of cadmium resistance by 10ug/ml of acriflavin was 28%, and by 20ug/ml of ethidium bromide was 47%, respectively.

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Adaptive Response in CHO Cells by Bleomycin, Mitomycin C and Cadmium (Bleomycin, Mitomycin C 및 Cadmium에 의한 CHO 세포의 적응반응)

  • 김양지;한정호;정해원
    • Journal of Environmental Health Sciences
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    • v.18 no.2
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    • pp.117-124
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    • 1992
  • Pretreatment with low concentration of Bleomycin and Cadmium rendered Chinese Hamster Ovary Cells more resistant to the induction of chromosome aberration by subsequent high concentration of same agent, however Mitomycin C did not function in that way. The cells pre-exposed to low dose of Cadmitim did not show cross-resistance to challenge dose of Mitomycin C for the induction of chromosome aberration, but cells pre-exposed to Bleomycin showed cross resistance. And the cells pre-exposed to low dose of Mitomycin C showed cross resistance to challenge of Bleomycin, but Cadmium did not.

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Isolation and Characterization of Pseudomonas sp. KM10, a Cadmium- and Mercury-resistant, and Phenol-degrading Bacterium

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.388-398
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    • 1998
  • A bacterium which is resistant to both mercury and cadmium, and also capable of utilizing phenol as a carbon and energy source, was isolated from the Kumho River sediments near Kangchang Bridge, Taegu, Korea. The isolate was labeled Pseudomonas sp. KM10 and characterized. The bacteria grew in 4 mM $CdCl_2$and in $70{\mu}M$ $HgCl_2$. The bacteria efficiently removed over 90% of 1 g/l phenol within 30 h. In the presence of 1.250 g/l phenol, the growth of the microorganism was slightly retarded and the microorganism could not tolerate 1.5 g/l phenol. Curing of plasmid from the bacteria was carried out to generate a plasmidless strain. Subsequent experiments localized the genes for phenol degradation in plasmid and the genes for mercury resistance and cadmium resistance on the chromosome. Dot hybridization and Southern hybridization under low stringent conditions were performed to identify the DNA homology. These results showed significant homologies between the some sequence of the chromosome of Pseudomonas sp. KM10 and merR of Shigella flexneri R 100, and between the some sequence of the chromosome of Pseudomonas sp. KM10 and cadA of Staphylococcus aureus pI258. The mechanism of cadmium resistance was efflux, similar to that of S. aureus pI258 cadA, and the mechanism of mercury resistance was volatilization, similar to that of S. flexneri R100 mer.

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Design remaining capacity calculation system of a nickel-cadmium battery by using fuzzy logic (퍼지로직을 이용한 니켈-카드뮴 축전지의 잔존용량 산출 알고리즘 제안)

  • Jang, Woong-Sung;Jeon, Sun-Yong
    • Proceedings of the KIEE Conference
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    • 2004.11c
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    • pp.355-357
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    • 2004
  • In this paper, to calculate accurate remaining volume, it presents how to figure out nickel-cadmium battery algorithm. A nickel-cadmium battery has widely been used in industrial field and to military. Recent high demands on the battery caused 'How to calculate accurate remaining volume is very important task to be solved. In this paper, it says it is useful using the terminal voltage change of the resistance that can be connected with the battery and the differentiation of the terminal voltage to calculate remaining volume of nickel-cadmium battery. And these can be used for volume inference data so that it is fuzzy based system which can be helpful to inference the remaining volume by the resistance of terminal voltage change. Because of electrochemical complexity, the volume calculating system is inferencing undirectly by experimentally built DB where as current the existing volume models are suffering to be adapted.

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Characterization of Azomonas agilis PY101, a Cadmium-Resistant Strain Isolated from Anyang Stream

  • You, Kyung-Man;Lee, Ji-Hyun;Kim, Jeong-Kook;Hah, Nam-Ju;Lee, Yung-Nok;Park, Yong-Keun
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.289-293
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    • 1996
  • A cadimium-resistant strain isolated from Anyang stream, Azomonas agilis PY101 exhibited strong resistance to 1000 ppm of cadmium ion $(Cd^{2+})$. A agilis PY101 also exhibited resistance to various antibiotics, such as amoxicillin, amplicillin, bacitracin, cefazolin, erythromycin, penicillin, tetracycline, and vancomycin. In the presence of $Cd^{2+}$, the growth of A. agilis PY101 started after an extended lag phase and produced a green-fluorescent pigment induced by cadmium. The dramatic decrease (approximately 400ppm) of concentration of $cd^{2+}$ in the culture medium during the growth phase of A. agilis PY101 was confirmed by the inductively coupled plasma-atomic emission spectrophotometer. Transmission electron microscopic analysis revealed that A. agilis PY 101 actively accumulated $Cd^{2+}$ in the cytoplasm.

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The Cadmium Biosorption Mechanism in Gram Negative Bacteria, Serratia marcescens (Gram 음성 세균인 Serratia marcescens에 의한 카드뮴 흡착 기작)

  • 이호용;민봉희;최영길
    • The Korean Journal of Ecology
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    • v.22 no.1
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    • pp.39-43
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    • 1999
  • Serratia marcescens, an enterobacterium of gram-negative bacteria, is characterized by resistance of the admium. Cadmium sensitive PM strain did not grow in the medium at cadmium concentration of 50 ppm. PA strain was induced to accommodate to cadmium by cultivating the mother strain (PC strain) in the medium with 50 ppm cadmium. As compared with PC and PM strains, PA strain revealed the excellent growth in cadmium media and accumulated four to five times higher cadmium concentration in cell than other strains. PA strain accumulated 23% of cadmium in cells when cultured in medium treated with 100 ppm cadmium and this cadmium was more accumulated in cytosol fractions than membrane fractions. Analysis by TEM indicated that cadmium was concentrated as a form of granule in cytosol. In protein patterns of cell after the treatment of cadmium, two inducible proteins (28 KDa and 64 KDa) and one reducible protein (45 KDa) were detected by SDS-PAGE. By Atomic Absorption Spectrophotometer, the amounts of cadmium attached to inducible proteins of 28 KDa and 64 KDa were 318.28 ㎍ and 325.37 ㎍ per gram of protein, respectively. It is assumed that these inducible proteins play an important role in the mechanism of cadmium accumulation in cells. A plasmid of 23Kbp was found in S. marcescens. The ability of resistance to cadmium in plasmid was confirmed by curing experiments.

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A Study on the Fabrication of Uranium-Cadmium Alloy and its Distillation Behavior (우라늄-카드뮴 합금의 제조 및 증류거동에 대한 연구)

  • Kim, Ji-Yong;Ahn, Do-Hee;Kim, Kwang-Rag;Paek, Seung-Woo;Kim, Si-Hyung
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.8 no.4
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    • pp.261-267
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    • 2010
  • The pyrometallurgical nuclear fuel recycle process, called pyroprocessing, has been known as a promising nuclear fuel recycling technology. Pyroprocessing technology is crucial to advanced nuclear systems due to increased nuclear proliferation resistance and economic efficiency. The basic concept of pyroprocessing is group actinide recovery, which enhances the nuclear proliferation resistance significantly. One of the key steps in pyroprocessing is "electrowinning" which recovers group actinides with lanthanide from the spent nuclear fuels. In this study, a vertical cadmium distiller was manufactured. The evaporation rate of pure cadmium in vertical cadmium distiller varied from 12.3 to $40.8g/cm^2/h$ within a temperature range of 773 923 K and pressure below 0.01 torr. Uranium - cadmium alloy was fabricated by electrolysis using liquid cadmium cathode in a high purity argon atmosphere glove box. The distillation behavior of pure cadmium and cadmium in uranium - cadmium alloy was investigated. The distillation behavior of cadmium from this study could be used to develop an actinide recovery process from a liquid cadmium cathode in a cadmium distiller.

Selection of Cadmium Resistant Cell Line from Calli of Nicotiana tabacum cv. BY4 (담배(Nicotiana tabacum L. cv. BY4)캘러스로부터 카드뮴 저항성 세포주의 선발)

  • 오승철;소웅영;조덕이;양덕춘
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.6
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    • pp.361-367
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    • 1997
  • This study was carried out to select cadmium resistant cell lines from leaf-derived calli of diploid and haploid of Nicotiana tabacum cv. BY4, for understanding adaptation mechanism of plants in cadmium contaminated environment. suspended cell clumps were plated onto selection medium containing 0 to 2,000 $\mu$M cadmium. Cadmium resistant colonies were formed on the selection medium after 3 or 4 weeks of culture. The minimum inhibition concentration (MIC) of cadmium on colony formation were 300 $\mu$M in diploid and 200 $\mu$M in haploid plants, respectively. In order to test the resistance to cadmium, selected cell line on MIC were transferred to medium containing high concentration of cadmium. The selected cell lines, especially haploid cell line, were resistant an the high concentration of cadmium. And dry weight, ash weight, and cadmium contents of cell were increased. These results indicated that the selected cell lines showed higher resistance of cadmium than control cells, and haploid plant is more resistant than diploid plant on medium with cadmium.

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Isolation of Cadmium Ion-resistant Bacteria and Resitance to Various Heavy Metals (카드봄 내성균의 분리와 각종 중금속에 대한 저항성의 연구)

  • Yeeh, Yeehn;Lee, Jong-Kun
    • Korean Journal of Microbiology
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    • v.17 no.2
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    • pp.49-57
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    • 1979
  • Cadimium ion-resistant microorganism was isolated from the sludge of wastewater. The physiological, morphological and other cultural data showed that this strain belonged to Citrobacter freudii. A clearcut distinction of growth among nutrient broth, typtic soy broth and synthetic medium was demonstrated. The resistant cells showed only slight mutagenic action. During the growth of bacterial population in resting state, the organisms reduced the initial level of resistance to cadmium ions when they were not kept in contact with cadmium ions in bacteral multiplication. And cadmium ion-resistant and cadmium ion-sensitive strain were found to show equal, lower or higher sensitivity to other heave metals.

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